Fluorimetric methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, β-hydroxybutyrate, glycerol) using a COBAS FARA centrifugal analyser

Intermediate products of the metabolism of glucose, fat and amino-acid are important in the evaluation of such metabolic disorders as diabetes mellitus, liver disease and metabolic acidosis. In the present study, methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, β-hydroxybutyrate and glycerol) have been adapted to a fast centrifugal analyzer: the COBAS FARA. Correlation coeffcients rangedfrom 0.90 to 0.99, compared to established manual spectrophotometric methods. Within-run coeffcients of variation (CVs) ranged between 2.9 and 8.8% at low levels, between 1.5 and 5.7% at medium levels and between 1.2 and 5.6% at high levels. Between-run CVs were between 4.0 and 15.0% at low levels, between 1.7 and 7.0% at medium levels and between 1.3 and 2.7% at high levels. These fluorimetric assays for the determination of intermediate metabolites on COBAS FARA (Roche) have a good sensitivity and precision, are less costly than manual methods and can be used on a routine basis

Collective Dipole Bremsstrahlung in Fusion Reactions

We estimate the dipole radiation emitted in fusion processes. We show that a classical bremsstrahlung approach can account for both the preequilibrium and the thermal photon emission. We give an absolute evaluation of the pre-equilibrium component due to the charge asymmetry in the entrance channel and we study the energy and mass dependence in order to optimize the observation. This dynamical dipole radiation could be a relevant cooling mechanism in the fusion path. We stress the interest in experiments with the new available radioactive beams.Comment: 4 pages (LATEX), 4 Postscript figures, minor text modification

Prompt dipole radiation in fusion reactions

The prompt gamma ray emission was investigated in the 16A MeV energy region by means of the 36,40Ar+96,92Zr fusion reactions leading to a compound nucleus in the vicinity of 132Ce. We show that the prompt radiation, which appears to be still effective at such a high beam energy, has an angular distribution pattern consistent with a dipole oscillation along the symmetry axis of the dinuclear system. The data are compared with calculations based on a collective bremsstrahlung analysis of the reaction dynamics

Probing the 6He halo structure with elastic and inelastic proton scattering

Proton elastic scattering and inelastic scattering to the first excited state of 6He have been measured over a wide angular range using a 40.9A MeV 6He beam. The data have been analyzed with a fully microscopic model of proton-nucleus scattering using 6He wave functions generated from large space shell model calculations. The inelastic scattering data show a remarkable sensitivity to the halo structure of 6He.Comment: 9 pages, 3 figures. RevTeX. Replaced figure 3 with updated figur

Transfer vs. Breakup in the interaction of the 7Be Radioactive Ion Beam with a 58Ni target at Coulomb barrier energies

We measured for the first time 7Be elastically scattered nuclei as well as 3,4He reaction products from a 58Ni target at 22.3 MeV beam energy. The data were analyzed within the optical model formalism to extract the total reaction cross section. Extensive kinematical, Distorted Wave Born Approximation (DWBA)and Continuum Discretized Coupled Channel (CDCC) calculations were performed to investigate the 3,4He originating mechanisms and the interplay between different reaction channels

Does the breakup process affect the reaction dynamics for the systems 17O, 17F + 58Ni at Coulomb barrier energies?

The scattering processes of two mirror projectiles, the well bound 17 O (Sn = 4.143 MeV) and the loosely bound radioactive 17 F (Sp = 0.600 MeV), on the proton closed shell target 58 Ni were measured at several energies around the Coulomb barrier. The experimental data were analyzed within the framework of the optical model to extract the reaction cross section and to investigate the role played by direct reaction channels at near-barrier energies. The comparison shows a similar behaviour for the two A = 17 projectiles despite their very different binding energies and suggests a rather small effect of the 17 F breakup channel on the reaction dynamics

Myocardial insulin resistance associated with chronic hypertriglyceridemia and increased FFA levels in Type 2 diabetic patients

We evaluated the influence of chronic hypertriglyceridemia and endothelial dysfunction on myocardial glucose uptake (MGU) in Type 2 diabetic patients without coronary heart disease. Patients were divided into two groups according to fasting triglyceride (TG) levels: 5.4 \ub1 1.1 and 1.5 \ub1 0.3 mmol/l for high- and normal-TG groups, respectively. Five subjects were assigned to the high-TG group and 11 to the normal-TG group. Age, gender, body mass index, systolic and diastolic blood pressure, glucose, insulin, HbA1c, cholesterol, and HDL cholesterol were similar in the two groups, whereas free fatty acid (FFA) levels were higher in the high-TG group basally and at the end of the clamp. Furthermore, five healthy subjects were subjected to the same protocol and used as the control group. MGU was assessed by using 18F-labeled 2-fluoro-2-deoxy-D-glucose under hyperglycemic- hyperinsulinemic conditions. Basal endothelin-1 and nitric oxide levels were significantly higher in the high-TG group than in the normal-TG and control groups, and cGMP and maximal postischemic vasodilation were significantly decreased in the high-TG group compared with the normal-TG and control groups. However, significant alterations were found in the same parameters in the normal-TG group compared with the control group. By the end of the hyperglycemic clamp, in the high-TG group, MGU was 3c40 and 65% of that in the normal-TG and control groups. MGU negatively correlated with TG, FFA, and endothelin-1, whereas a positive correlation was found with cGMP and maximal postischemic vasodilation. In conclusion, increased TG and FFA levels are risks, in addition to Type 2 diabetes mellitus, for myocardial insulin resistance, endothelial dysfunction, and alteration of nitric oxide/cGMP levels

Fluorimetric methods for the measurement of intermediate metabolites II-hydroxybutyrate, glycerol)using a COBAS FARA centrifugal analyser

Within-run coeffcients of variation (CVs) ranged between 2"9 and 8"80//o at low levels, between 1"5 and 5"70//0 at medium levels and between 1"2 and 5"6o//0 at high levels. Between-run CVs were between 4"0 and 15"0% at low levels, between 1"7 and 7"0% at medium levels and between 1"3 and 2"70//0 at high levels. Thesejquorimetric assays for the determination of intermediate metabolites on COBAS FARA (Roche) have a good sensitivity andprecision, are less costly than manual methods and can be used on a routine basis