Antitumor Activity and Reductive Stress by Platinum(II) N-Heterocyclic Carbenes based on Guanosine**

Funding Information: We thank Fernanda Murtinheira for the support on the autophagy measurements. This work was supported by FCT – Fundação para a Ciência e a Tecnologia, I.P., through MOSTMICRO‐ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). M.I.P.S.L. was supported by fellowships PD/BD/135483/2018 and COVID/BD/152502/2022 from FCT. A.P. acknowledges the contract CEECINST/00102/2018. The NMR spectra were acquired at CERMAX–ITQB, supported by Infrastructure Project No. 022161 (co‐financed by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC). C.S.B. Gomes acknowledges the XTAL – Macromolecular Crystallography group for granting access to the X‐ray diffractometer. X‐ray infrastructure financed by FCT‐MCTES through project RECI/BBBBEP/0124/2012. FH was supported by Centre grants from FCT to the BioISI Research Unit (Refs. UIDB/04046/2020 and UIDP/04046/2020) and the Microscopy facility at FCUL (as a node of the Portuguese Platform of BioImaging, reference PPBI‐POCI‐01‐0145‐FEDER‐022122), and by individual grants through FCT (Ref. PTDC/FIS‐MAC/2741/2021) and the ARSACS Foundation (Canada). Maria Turos‐Cabal was supported by fellowship from the Spanish Association Against Cancer (AECC, SV‐19‐AECC‐FPI) and the Consejería de Economía y Empleo del Principado de Asturias (FICYT, Severo‐Ochoa BP20‐073). Publisher Copyright: © 2023 Wiley-VCH GmbH.Platinum(II) complexes bearing N-heterocyclic carbenes based guanosine and caffeine have been synthesized by unassisted C−H oxidative addition, leading to the corresponding trans-hydride complexes. Platinum guanosine derivatives bearing triflate as counterion or bromide instead of hydride as co-ligand were also synthesized to facilitate correlation between structure and activity. The hydride compounds show high antiproliferative activity against all cell lines (TC-71, MV-4-11, U-937 and A-172). Methyl Guanosine complex 3, bearing a hydride ligand, is up to 30 times more active than compound 4, with a bromide in the same position. Changing the counterion has no significant effect in antiproliferative activity. Increasing bulkiness at N7, with an isopropyl group (compound 6), allows to maintain the antiproliferative activity while decreasing toxicity for non-cancer cells. Compound 6 leads to an increase in endoplasmic reticulum and autophagy markers on TC71 and MV-4-11 cancer cells, induces reductive stress and increases glutathione levels in cancer cells but not in non-cancer cell line HEK-293.preprintinpres

Effect of Poultry Manure-Derived Compost on the Growth of eucalypts spp. Hybrid Clones

Interspecific hybrids of E. grandis × E. camaldulensis were generated to widen the plantation area. The aim of this study was to assess root capability and development for six different clones of eucalyptus grown in substrates made with three different composts derived from poultry manure. A factorial design was used to assess the effect of different composts on six growth variables. The analysis detected a greater effect from the genotype than the substrate. E. grandis × E. camaldulensis hybrid vegetative propagation was successful in alternative substrates formulated from composted poultry manure. GC8 was the genotype that showed the greatest differences for four the different variables among the substrates, being both the most sensitive and the one with the highest values for all parameters measured. The hybrids' vegetative propagation was determined in alternative substrates formulated from poultry manure compost. The physicochemical characteristics of substrates composed of pine bark and sawdust provided adequate conditions for the growth of eucalyptus. GC8 was the genotype most sensitive to the use of different substrates, showing significant differences in the ratio of roots/callus, radicular dry weight, and cutting dry weight. These clones might be a good option for evaluating compost-based substrates for forestry applications

Pseudogap Formation in the Symmetric Anderson Lattice Model

We present self-consistent calculations for the self-energy and magnetic susceptibility of the 2D and 3D symmetric Anderson lattice Hamiltonian, in the fluctuation exchange approximation. At high temperatures, strong f-electron scattering leads to broad quasiparticle spectral functions, a reduced quasiparticle band gap, and a metallic density of states. As the temperature is lowered, the spectral functions narrow and a pseudogap forms at the characteristic temperature $T_x$ at which the width of the quasiparticle spectral function at the gap edge is comparable to the renormalized activation energy. For $T << T_x$, the pseudogap is approximately equal to the hybridization gap in the bare band structure. The opening of the pseudogap is clearly apparent in both the spin susceptibility and the compressibility.Comment: RevTeX - 14 pages and 7 figures (available on request), NRL-JA-6690-94-002

Primordial black holes in braneworld cosmologies: Accretion after formation

We recently studied the formation and evaporation of primordial black holes in a simple braneworld cosmology, namely Randall-Sundrum Type II. Here we study the effect of accretion from the cosmological background onto the black holes after formation. While it is generally believed that in the standard cosmology such accretion is of negligible importance, we find that during the high-energy regime of braneworld cosmology accretion can be the dominant effect and lead to a mass increase of potentially orders of magnitude. However, unfortunately the growth is exponentially sensitive to the accretion efficiency, which cannot be determined accurately. Since accretion becomes unimportant once the high-energy regime is over, it does not affect any constraints expressed at the time of black hole evaporation, but it can change the interpretation of those constraints in terms of early Universe formation rates.Comment: 6 pages RevTeX4 file. Extension to discussion of thermal balance and grey-body factor

Magnetic flux jumps in textured Bi2Sr2CaCu2O(8+d)

Magnetic flux jumps in textured Bi2Sr2CaCu2O(8+d) have been studied by means of magnetization measurements in the temperature range between 1.95 K and Tc, in an external magnetic field up to 9 T. Flux jumps were found in the temperature range 1.95 K - 6 K, with the external magnetic field parallel to the c axis of the investigated sample. The effect of sample history on magnetic flux jumping was studied and it was found to be well accounted for by the available theoretical models. The magnetic field sweep rate strongly influences the flux jumping and this effect was interpreted in terms of the influence of both flux creep and the thermal environment of the sample. Strong flux creep was found in the temperature and magnetic field range where flux jumps occur suggesting a relationship between the two. The heat exchange conditions between the sample and the experimental environment also influence the flux jumping behavior. Both these effects stabilize the sample against flux instabilities, and this stabilizing effect increases with decreasing magnetic field sweep rate. Demagnetizing effects are also shown to have a significant influence on flux jumping.Comment: 10 pages, 6 figures, RevTeX4, submitted to Phys. Rev.

Lemaitre-Tolman-Bondi model and accelerating expansion

I discuss the spherically symmetric but inhomogeneous Lemaitre-Tolman- Bondi (LTB) metric, which provides an exact toy model for an inhomogeneous universe. Since we observe light rays from the past light cone, not the expansion of the universe, spatial variation in matter density and Hubble rate can have the same effect on redshift as acceleration in a perfectly homogeneous universe. As a consequence, a simple spatial variation in the Hubble rate can account for the distant supernova data in a dust universe without any dark energy. I also review various attempts towards a semirealistic description of the universe based on the LTB model.Comment: Invited Review for a special Gen. Rel. Grav. issue on Dark Energy. 17 pages, 3 figure

Green function techniques in the treatment of quantum transport at the molecular scale

The theoretical investigation of charge (and spin) transport at nanometer length scales requires the use of advanced and powerful techniques able to deal with the dynamical properties of the relevant physical systems, to explicitly include out-of-equilibrium situations typical for electrical/heat transport as well as to take into account interaction effects in a systematic way. Equilibrium Green function techniques and their extension to non-equilibrium situations via the Keldysh formalism build one of the pillars of current state-of-the-art approaches to quantum transport which have been implemented in both model Hamiltonian formulations and first-principle methodologies. We offer a tutorial overview of the applications of Green functions to deal with some fundamental aspects of charge transport at the nanoscale, mainly focusing on applications to model Hamiltonian formulations.Comment: Tutorial review, LaTeX, 129 pages, 41 figures, 300 references, submitted to Springer series "Lecture Notes in Physics

Azimuthal anisotropy and correlations in p+p, d+Au and Au+Au collisions at 200 GeV

We present the first measurement of directed flow ($v_1$) at RHIC. $v_1$ is found to be consistent with zero at pseudorapidities $\eta$ from -1.2 to 1.2, then rises to the level of a couple of percent over the range $2.4 < |\eta| < 4$. The latter observation is similar to data from NA49 if the SPS rapidities are shifted by the difference in beam rapidity between RHIC and SPS. Back-to-back jets emitted out-of-plane are found to be suppressed more if compared to those emitted in-plane, which is consistent with {\it jet quenching}. Using the scalar product method, we systematically compared azimuthal correlations from p+p, d+Au and Au+Au collisions. Flow and non-flow from these three different collision systems are discussed.Comment: Quark Matter 2004 proceeding, 4 pages, 3 figure

Azimuthal anisotropy: the higher harmonics

We report the first observations of the fourth harmonic (v_4) in the azimuthal distribution of particles at RHIC. The measurement was done taking advantage of the large elliptic flow generated at RHIC. The integrated v_4 is about a factor of 10 smaller than v_2. For the sixth (v_6) and eighth (v_8) harmonics upper limits on the magnitudes are reported.Comment: 4 pages, 6 figures, contribution to the Quark Matter 2004 proceeding

Partonic flow and ϕ\phi-meson production in Au+Au collisions at sNN\sqrt{s_{NN}} = 200 GeV

We present first measurements of the $\phi$-meson elliptic flow ($v_{2}(p_{T})$) and high statistics $p_{T}$ distributions for different centralities from $\sqrt{s_{NN}}$ = 200 GeV Au+Au collisions at RHIC. In minimum bias collisions the $v_{2}$ of the $\phi$ meson is consistent with the trend observed for mesons. The ratio of the yields of the $\Omega$ to those of the $\phi$ as a function of transverse momentum is consistent with a model based on the recombination of thermal $s$ quarks up to $p_{T}\sim 4$ GeV/$c$, but disagrees at higher momenta. The nuclear modification factor ($R_{CP}$) of $\phi$ follows the trend observed in the $K^{0}_{S}$ mesons rather than in $\Lambda$ baryons, supporting baryon-meson scaling. Since $\phi$-mesons are made via coalescence of seemingly thermalized $s$ quarks in central Au+Au collisions, the observations imply hot and dense matter with partonic collectivity has been formed at RHIC.Comment: 6 pages, 4 figures, submit to PR