Glomerular filtration is reduced by high tidal volume ventilation in an in vivo healthy rat model

Mechanical ventilation has been associated with organ failure in patients with acute respiratory distress syndrome. The present study examines the effects of tidal volume (V T) on renal function using two V T values (8 and 27 mL/kg) in anesthetized, paralyzed and mechanically ventilated male Wistar rats. Animals were randomized into two groups of 6 rats each: V T8 (V T, 8 mL/kg; 61.50 ± 0.92 breaths/min; positive end-expiratory pressure, 3.0 cmH2O; peak airway pressure (PAW), 11.8 ± 2.0 cmH2O), and V T27 (V T, 27 mL/kg; 33.60 ± 1.56 breaths/min; positive end-expiratory pressure, none, and PAW, 22.7 ± 4.0 cmH2O). Throughout the experiment, mean PAW remained comparable between the two groups (6.33 ± 0.21 vs 6.50 ± 0.22 cmH2O). For rats in the V T27 group, inulin clearance (mL·min-1·body weight-1) decreased acutely after 60 min of mechanical ventilation and even more significantly after 90 min, compared with baseline values (0.60 ± 0.05 and 0.45 ± 0.05 vs 0.95 ± 0.07; P < 0.001), although there were no differences between groups in mean arterial pressure or gasometric variables. In the V T8 group, inulin clearance at 120 min of mechanical ventilation remained unchanged in relation to baseline values (0.72 ± 0.03 vs 0.80 ± 0.05). The V T8 and V T27 groups did not differ in terms of serum thiobarbituric acid reactive substances (3.97 ± 0.27 vs 4.02 ± 0.45 nmol/mL) or endothelial nitric oxide synthase expression (94.25 ± 2.75 vs 96.25 ± 2.39%). Our results show that glomerular filtration is acutely affected by high tidal volume ventilation but do not provide information about the mechanism

Air pollution aggravates renal ischaemia-reperfusion-induced acute kidney injury

Chronic kidney disease (CKD) has emerged as a significant global public health concern. Recent epidemiological studies have highlighted the link between exposure to fine particulate matter (PM2.5) and a decline in renal function. PM2.5 exerts harmful effects on various organs through oxidative stress and inflammation. Acute kidney injury (AKI) resulting from ischaemia–reperfusion injury (IRI) involves biological processes similar to those involved in PM2.5 toxicity and is a known risk factor for CKD. The objective of this study was to investigate the impact of PM2.5 exposure on IRI-induced AKI. Through a unique environmentally controlled setup, mice were exposed to urban PM2.5 or filtered air for 12 weeks before IRI followed by euthanasia 48 h after surgery. Animals exposed to PM2.5 and IRI exhibited reduced glomerular filtration, impaired urine concentration ability, and significant tubular damage. Further, PM2.5 aggravated local innate immune responses and mitochondrial dysfunction, as well as enhancing cyclic GMP–AMP synthase-stimulator of interferon genes (cGAS–STING) pathway activation. This increased renal senescence and suppressed the anti-ageing protein klotho, leading to early fibrotic changes. In vitro studies using proximal tubular epithelial cells exposed to PM2.5 and hypoxia/reoxygenation revealed heightened activation of the STING pathway triggered by cytoplasmic mitochondrial DNA, resulting in increased tubular damage and a pro-inflammatory phenotype. In summary, our findings imply a role for PM2.5 in sensitising proximal tubular epithelial cells to IRI-induced damage, suggesting a plausible association between PM2.5 exposure and heightened susceptibility to CKD in individuals experiencing AKI. Strategies aimed at reducing PM2.5 concentrations and implementing preventive measures may improve outcomes for AKI patients and mitigate the progression from AKI to CKD. © 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. Immunopathology of vascular and renal diseases and of organ and celltransplantatio

Suplementação de aminoácidos para redução da proteína bruta em dietas para leitões desmamados aos 21 dias de idade

Avaliou-se o efeito da suplementação de aminoácidos industriais para redução de proteína bruta (PB) em dietas para leitões desmamados aos 21 dias de idade sobre o desempenho e sobre as variáveis morfofisiológicas, utilizando-se 126 leitões com peso inicial de 6,05±0,35kg. Os animais foram distribuídos em delineamento experimental de blocos ao acaso, com seis tratamentos (24,0; 23,0; 22,0; 21,0; 20,0; 19,0% de PB), sete repetições e três animais por unidade experimental. Não houve efeito significativo da redução de proteína bruta da dieta sobre o ganho de peso médio diário, o consumo de ração diário e a conversão alimentar, e o pH do estômago também não sofreu influência, mas houve diminuição do pH do duodeno. Os pesos relativos do baço e do fígado, a altura de vilosidades e a profundidade de criptas no duodeno, jejuno e íleo não foram influenciados, enquanto o peso do pâncreas diminuiu com a redução da proteína bruta da dieta

Performance Comparison Of Hybrid 1-d Wdm/ocdma And 2-d Ocdma Towards Future Access Network Migration Scenario

In this paper, we provide a performance comparison and analysis between a hybrid 1-D WDM/OCDMA system and a 2-D WHTS OCDMA system. In the hybrid system, OOC is employed as users code sequence of the OCDMA dimension. The simulation results show that the hybrid system presents better performance than the 2-D WHTS OCDMA system, and show also that under a standard BER scenario, i.e., BER 10 9, the hybrid system can support at least 75% more simultaneous users than the 2-D OCDMA system for the analyzed case study. The analysis suggests that the hybrid 1-D WDM/OCDMA system is a qualified migration scenario since it can provide a smooth migration path from current networks towards OCDMA networks. © 2012 IEEE.Beyranvand, H., Salehi, J.A., Multirate and multi-quality-of-service passive optical network based on hybrid WDM/OCDM system (2011) IEEE Communications Magazine, 49, pp. 39-44Kitayama, K., Wang, X., Wada, N., OCDMA over WDM PON solution path to gigabit-symmetric FTTH (2006) J. Lightwave Technol., 24, pp. 1654-1662. , AprSanches, A.L., Dos Reis Jr., J.V., Borges, B.-H.V., Analysis of high-speed optical wavelength/time CDMA networks using pulse-position modulation and forward error correction techniques (2009) J. Lightwave Technol., 27 (22), pp. 5134-5144. , NovRaddo, T.R., Sanches, A.L., Dos Reis Jr., J.V., Borges, B.-H.V., A new approach for evaluating the BER of a multirate, multiclass OFFH-CDMA system (2012) IEEE Communications Letters, 16 (2), pp. 259-261. , FebYang, G.-C., Kwong, W.C., Performance comparison of multiwavelength CDMA and WDMA+OCDMA for fiber-optics networks (2002) Trans. of Communications, 45, pp. 1426-1434Shurong, S., Yin, H., Wang, Z., A new family of 2-D optical orthogonal codes and analysis of its performance in optical CDMA access networks (2006) J. Lightwave Technol., 24 (4), pp. 1646-1652. , AprYin, H., Richardson, D., (2007) Optical Code Division Multiple Access Communication, p. 123. , Ed. SpringerWang, X., Wada, N., Hamanaka, T., Miyazaki, T., Cincotti, G., Kitayama, K., OCDMA over WDM transmission (2007) Proc. ICTON 2007, , Rome, Italy, July, paper Tu.B1.

Digestible lysine levels in diets supplemented with ractopamine

In order evaluate digestible lysine levels in diets supplemented with 20 ppm of ractopamine on the performance and carcass traits, 64 barrows with high genetic potential at finishing phase were allotted in a completely randomized block design with four digestible lysine levels (0.80, 0.90, 1.00, and 1.10%), eight replicates and two pigs per experimental unit. Initial body weight and pigs' kinship were used as criteria in the blocks formation. Diets were mainly composed of corn and soybean meal supplemented with minerals, vitamins and amino acids to meet pigs' nutritional requirements at the finishing phase, except for digestible lysine. No effect of digestible lysine levels was observed in animal performance. The digestible lysine intake increased linearly by increasing the levels of digestible lysine in the diets. Carcass traits were not influenced by the dietary levels of digestible lysine. The level of 0.80% of digestible lysine in diets supplemented with 20 ppm ractopamine meets the nutritional requirements of castrated male pigs during the finishing phase