Interviews with Charles E. Wood and the Golden age Club

Interviews with Charles E. Wood and the Golden age Club on May 5, 1963 by Charles W. Wood. 00:00:16 - Introduction regarding his birth in Kentucky in 1892 and his work in the coal mines as a young teenager. 00:01:20 - Description of the coal mines 00:04:30 - Length of career as a coal miner 00:05:26 - John L. Lewis and Union benefits 00:08:20 - Working conditions 00:11:06 - Specifics of the job 00:12:53 - Mining accidents, cave-ins, and a gas explosion in 1910 00:15:23 - Death of Shirley Kittinger in a cave-in 00:16:34 - Strikes 00:18:59 - Types of coal and details about the mines 00:24:40 - Various jobs he\u27s held 00:25:00 - Timbering and the squeeze 00:27:06 - Flood of 1937, working conditions, and salary 00:31:10 - Interview with the Golden Age Club on March 18, 1963 in Hays, KS by Samuel J. Sackett 00:31:30 - Mingled discussion 00:32:03 - A version of the town naming of Wamego, KS by Mrs. Frank Holcomb of Hays, KS 00:33:27 - Irish relatives coming to visit in Kansas 00:35:17 - Mingled discussion 00:37:03 - Using the moon phase and the zodiac to make farming decisions 00:39:33 - William D. Walburn, experiences moving to Kansas from Oklahoma in a covered wagon in 1897. 00:46:33 - Weather predictions 00:48:01 - Reading fortunes in coffee grounds and tea leaves 00:51:41 - Using bread board scrapings to choose a wife 00:52:54 - Recipe for boardfish 00:53:39 - Stone posts, stone houses, and the stone museum 00:57:04 - Mingled discussion 00:59:24 - Mule jokes 01:02:15 - Fish stories 01:04:13 - Mingled discussion 01:08:31 - Making hominy 01:08:42 - Mingled discussionhttps://scholars.fhsu.edu/sackett/1081/thumbnail.jp

Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b

During embryonic development, regulation of gene expression is key to creating the many subtypes of cells that an organism needs throughout its lifetime. Recent work has shown that maternal genetics and environmental factors have lifelong consequences on diverse processes ranging from immune function to stress responses. The RE1-silencing transcription factor (Rest) is a transcriptional repressor that interacts with chromatin-modifying complexes to repress transcription of neural specific genes during early development. Here we show that in zebrafish, maternally supplied rest regulates expression of target genes during larval development and has lifelong impacts on behavior. Larvae deprived of maternal rest are hyperactive and show atypical spatial preferences. Adult male fish deprived of maternal rest present with atypical spatial preferences in a novel environment assay. Transcriptome sequencing revealed 158 genes that are repressed by maternal rest in blastula stage embryos. Furthermore, we found that maternal rest is required for target gene repression until at least 6 dpf. Importantly, disruption of the RE1 sites in either snap25a or snap25b resulted in behaviors that recapitulate the hyperactivity phenotype caused by absence of maternal rest. Both maternal rest mutants and snap25a RE1 site mutants have altered primary motor neuron architecture that may account for the enhanced locomotor activity. These results demonstrate that maternal rest represses snap25a/b to modulate larval behavior and that early Rest activity has lifelong behavioral impacts

Optimizing a bacterial sRNA scaffold for targeting multiple mRNAs, filtering off- target mRNA interactions, and balancing metabolic pathway flux

RNA is central to gene expression control in cells, and yet the small regulatory RNAs (sRNAs) of bacteria are still in the early stages of development as synthetic biology tools. The ubiquity and diversity of sRNAs in bacteria bodes well for engineering synthetic sRNA control of metabolic pathways, particularly in organisms with poorly developed genetic tools. These sRNAs regulate mRNA targets by RNA:RNA base-pairing interactions, and sRNAs have been retargeted to regulate non-native mRNAs for metabolic engineering applications.1-3 However, an ongoing concern about sRNAs as tools is their potential for hybridizing to off-target mRNAs. Here we describe the development, optimization and implementation of a structured sRNA scaffold with improved target discrimination relative to an unstructured antisense sRNA scaffold. Native DsrA sRNA4,5 contains two striking stem-loop antisense motifs that use antisense base-pairing to coordinately regulate translation of two E. coli mRNA targets. Previously6 we created a genetic system for retargeting DsrA simultaneously to two non-native mRNA targets in E. coli. Next, we expressed a retargeted E. coli sRNA variant in C. acetobutylicum cultures to improve n-butanol biofuel fermentation yield and selectivity. We achieved this goal by retargeting a DsrA sRNA variant to tune-down expression of an essential clostridial hydrogenase and increase NADH levels in the fermentation culture. Finally, we used this E. coli sRNA genetic system to demonstrate that the stem-loop antisense “fingerloop” structures can be configured to exclude certain off-target mRNA interactions. This fingerloop antisense motif constitutes a very promising programmable target–mRNA control element that is modular, discriminating, and portable between organisms. Since fine-tuning and balancing metabolic pathway flux is an important scale-up parameter, this nanoscale sRNA tool should be particularly useful in industrial scale bacterial fermentations of biofuels and specialty chemicals. Please click Additional Files below to see the full abstract

Ultrafast depolarization of the fluorescence in a conjugated polymer

The effect of the extent of pi electron conjugation on the primary photophysics in semiconducting polymers is reported. A rapid depolarization of photoluminescence and transient absorption, which indicates a reorientation of the transition dipole moment by similar to 30 degrees on a sub-100 fs time scale, is observed in the fully conjugated polymer poly[2-(2'-ethylhexyloxy)-5-methoxy-1,4-phenylenevinylene] (MEH-PPV). In contrast, partially conjugated polymers exhibit a much slower depolarization. The results reveal rapid changes of exciton delocalization in the fully conjugated MEH-PPV driven by structural relaxation

Multi-color Optical and NIR Light Curves of 64 Stripped-Envelope Core-Collapse Supernovae

We present a densely-sampled, homogeneous set of light curves of 64 low redshift (z < 0.05) stripped-envelope supernovae (SN of type IIb, Ib, Ic and Ic-bl). These data were obtained between 2001 and 2009 at the Fred L. Whipple Observatory (FLWO) on Mt. Hopkins in Arizona, with the optical FLWO 1.2-m and the near-infrared PAIRITEL 1.3-m telescopes. Our dataset consists of 4543 optical photometric measurements on 61 SN, including a combination of UBVRI, UBVr'i', and u'BVr'i', and 2142 JHKs near-infrared measurements on 25 SN. This sample constitutes the most extensive multi-color data set of stripped-envelope SN to date. Our photometry is based on template-subtracted images to eliminate any potential host galaxy light contamination. This work presents these photometric data, compares them with data in the literature, and estimates basic statistical quantities: date of maximum, color, and photometric properties. We identify promising color trends that may permit the identification of stripped-envelope SN subtypes from their photometry alone. Many of these SN were observed spectroscopically by the CfA SN group, and the spectra are presented in a companion paper (Modjaz et al. 2014). A thorough exploration that combines the CfA photometry and spectroscopy of stripped-envelope core-collapse SN will be presented in a follow-up paper.Comment: 26 pages, 17 figures, 8 tables. Revised version resubmitted to ApJ Supplements after referee report. Additional online material is available through http://cosmo.nyu.edu/SNYU

Demographics and management of dog bite victims at a level two hospital in KwaZulu-Natal

Introduction. Dog bites are a significant cause of morbidity and mortality worldwide, particularly where rabies is endemic. There is also a significant financial burden attached to prophylactic treatment to diminish the risk of rabies infection. KwaZulu-Natal (KZN) has a high incidence of human rabies yet little is known about the demographics of dog bites in the province. Objectives. To analyse the demographics of dog bites in Northern KZN. Methods. Records of all dog bites presenting to the main referral hospital in Northern KZN between August 2007 and September 2011 were analysed. Results. We collected data for 821 instances of dog bite. Male children aged 6 - 10 years are most likely to present with dog bites, while women >40 years are more likely to present than men in the same age bracket. Whilst initial vaccine administration is high (98%) with all grades of bite, only 82% of grade 3 bites receive immunoglobulin. Conclusion. Our results correlate well with two large studies of the demographics of dog bites, but are the first to show a reverse in male preponderance of presentations above the age of 40 years. Reasons for low rates of immunoglobulin administration in grade 3 bites are discussed. Finally, methods are suggested to improve data collection and the care of patients presenting with dog bites

The role of species composition in the emergence of alternate vegetation states in a temperate rainforest system

Context: Forest systems are dynamic and can alternate between alternative stable states in response to climate, disturbance and internal abiotic and biotic conditions. Switching between states depends on the crossing of critical thresholds and the establishment of feedbacks that drive (and maintain) changes in ecosystem functioning. The nature of these thresholds and the workings of these feedbacks have been well-researched, however, the factors that instigate movement toward and across a threshold remain poorly understood. Objectives: In this paper, we explore the role of species composition in initiating ecosystem state change in a temperate landscape mosaic of fire-prone and fire-sensitive vegetation systems.Methods: We construct two 12-kyr palaeocecological records from two proximal (230 m apart) sites in Tasmania, Australia, and apply the Alternative Stable States model as a framework to investigate ecosystem feedbacks and resilience threshold dynamics. Results: Our results indicate that, in this system, invasion by pyrogenic Eucalyptus species is a key factor in breaking down negative (stabilising) feedbacks that maintain pyrophobic sub-alpine rainforest.Conclusions: We conclude that the emergence of an alternative stable pyrogenic state in these relic rainforest systems depends on the extent of pyrophytic species within the system. These findings are critical for understanding resilience in forest ecosystems under future climate and land management changes and are relevant to fire-adapted cool-temperate ecosystems globally

Festschrift Symposium: Honoring Professor Samuel Pillsbury

The Loyola of Los Angeles Law Review is pleased to publish this Festschrift Symposium Honoring Professor Samuel Pillsbury. The following is an edited transcript of the live symposium held at LMU Loyola Law School on Friday, March 25, 2022

Intraperitoneal delivery of paclitaxel by poly(ether-anhydride) microspheres effectively suppresses tumor growth in a murine metastatic ovarian cancer model

Intraperitoneal (IP) chemotherapy is more effective than systemic chemotherapy for treating advanced ovarian cancer, but is typically associated with severe complications due to high dose, frequent administration schedule, and use of non-biocompatible excipients/delivery vehicles. Here, we developed paclitaxel (PTX)-loaded microspheres composed of di-block copolymers of poly(ethylene glycol) and poly(sebacic acid) (PEG-PSA) for safe and sustained IP chemotherapy. PEG-PSA microspheres provided efficient loading (∼13 % w/w) and prolonged release (∼13 days) of PTX. In a murine ovarian cancer model, a single dose of IP PTX/PEG-PSA particles effectively suppressed tumor growth for more than 40 days and extended the median survival time to 75 days compared to treatments with Taxol® (47 days) or IP placebo particles (34 days). IP PTX/PEG-PSA was well tolerated with only minimal to mild inflammation. Our findings support PTX/PEG-PSA microspheres as a promising drug delivery platform for IP therapy of ovarian cancer and potentially other metastatic peritoneal cancers