Field emission of cesium ions

Field emission of cesium ions from positive electrode formulated for electric field range that binds ions to electrode surfac

Growth of wildtype and mutant E. coli strains in minimal media for optimal production of nucleic acids for preparing labeled nucleotides

Since RNAs lie at the center of most cellular processes, there is a need for synthesizing large amounts of RNAs made from stable isotope-labeled nucleotides to advance the study of their structure and dynamics by nuclear magnetic resonance (NMR) spectroscopy. A particularly effective means of obtaining labeled nucleotides is to harvest these nucleotides from bacteria grown in defined minimal media supplemented with 15NH4Cl and various carbon sources. Given the high cost of carbon precursors required for labeling nucleic acids for NMR studies, it becomes important to evaluate the optimal growth for commonly used strains under standard minimal media conditions. Such information is lacking. In this study, we characterize the growth for Escherichia coli strains K12, K10zwf, and DL323 in three minimal media with isotopic-labeled carbon sources of acetate, glycerol, and glycerol combined with formate. Of the three media, the LeMaster-Richards and the Studier media outperform the commonly used M9 media and both support optimal growth of E. coli for the production of nucleotides. However, the growth of all three E. coli strains in acetate is reduced almost twofold compared to growth in glycerol. Analysis of the metabolic pathway and previous gene array studies help to explain this differential growth in glycerol and acetate. These studies should benefit efforts to make selective 13C-15N isotopic-labeled nucleotides for synthesizing biologically important RNAs

Global linear stability analysis of kinetic Trapped Ion Mode (TIM) turbulence in tokamak plasma using spectral method

Trapped ion modes (TIM) which belong to the family of ion temperature gradient (ITG) modes, is one of the important ingredients in heat turbulent transport at the ion scale in tokamak plasmas. It is essential to properly estimate their linear growth rate to understand their influence on ion-scale turbulent transport. A global linear analysis of a reduced gyro-bounce kinetic model for trapped particle modes is performed, and a spectral method is proposed to solve the dispersion relation. Importantly, the radial profile of the particle drift velocity is taken into account in the linear analysis by considering the exact magnetic flux {\psi} dependency of the equilibrium Hamiltonian H_{eq}({\psi}) in the quasi-neutrality equation and equilibrium gyro-bounce averaged distribution function F_{eq} . Using this spectral method, linear growth-rates of TIM instability in presence of different temperature profiles and precession frequencies of trapped ions, with an approximated constant Hamiltonian and the exact {\psi} dependent equilibrium Hamiltonian, are investigated. The growth-rate depends on the logarithmic gradient of temperature \kappa_{T} , density \kappa_{n} and equilibrium Hamiltonian \kappa_{\Lambda} . With the exact {\psi} dependent Hamiltonian, the growth rates and potential profiles are modified significantly, compared to the cases with approximated constant Hamiltonian. All the results from the global linear analysis agree with a semi-Lagrangian based linear Vlasov solver with a good accuracy. This spectral method is very fast and requires very less computation resources compared to a linear version of Vlasov-solver based on a semi-Lagrangian scheme

Effect of temperature anisotropy on the dynamics of geodesic acoustic modes

In this work, we revisit the linear gyro-kinetic theory of geodesic acoustic modes (GAMs) and derive a general dispersion relation for an arbitrary equilibrium distribution function of ions. A bi-Maxwellian distribution of ions is then used to study the effects of ion temperature anisotropy on GAM frequency and growth rate. We find that ion temperature anisotropy yields sensible modifications to both the GAM frequency and growth rate as both tend to increase with anisotropy and these results are strongly affected by the electron to ion temperature ratio

Cryptococcal meningitis associated HIV infection in the Donka national hospital in Conakry (Guinea)

Background Cryptococcal meningitis (CM) is an infection of the brain parenchyma and subarachnoid space by the encapsulated saprophyte yeast organisms such as Cryptococcus neoformans. Over the last twenty years, HIV has created a large and severely immune compromisized population in whom C. neoformans is a dangerous opportunistic infection. In Guinea, the prevalence of CM is unknown. We hypothesized that the occurrence of CM correlates with AIDS/ HIV prevalence.Method This retrospective observational study was carried out at the national Hospital of Conakry (Guinea) between 2001 and 2002. We describe here the epidemiological and clinical and biological characteristics of CM disease in our national hospital.Results Our data show that, 28.6 % of HIV patients with neurological symptoms had Cryptococcus neoformans in their CSF by using Indian ink staining. The median age was 36±3 years and sex ratio (M/F) was 1.8. The major complaints were fever and cephalgia, giddiness while the major complications were altered consciousness and hemiplegia. CSF was clear with low level of glucose and higher level of albumin. The means of lymphocytes in CSF was 8±2/mm3.Conclusion This data therefore becomes relevant in not only focusing of neurological symptoms associated with HIV to be toxoplasmosis but the possibility of C neoformans in these patients; particularly when they present symptoms such as headaches, giddiness and sniff neck etc. This can easily be carried out with Indian ink staining technique

Nonlinear interaction of Alfv\'enic instabilities and turbulence via the modification of the equilibrium profiles

Nonlinear simulations of Alfv\'en modes (AM) driven by energetic particles (EP) in the presence of turbulence are performed with the gyrokinetic particle-in-cell code ORB5. The AMs carry a heat flux, and consequently they nonlinearly modify the plasma temperature profiles. The isolated effect of this modification on the dynamics of turbulence is studied, by means of electrostatic simulations. We find that turbulence is reduced when the profiles relaxed by the AM are used, with respect to the simulation where the unperturbed profiles are used. This is an example of indirect interaction of EPs and turbulence. First, an analytic magnetic equilibrium with circular concentric flux surfaces is considered as a simplified example for this study. Then, an application to an experimentally relevant case of ASDEX Upgrade is discussed

Plan de negocios para una empresa de gesti?n y control de servicios integrados de telecomunicaciones para la MYPE de Lima Metropolitana

El desarrollo del presente plan de negocios ha podido demostrar que la creaci?n de una empresa que brinde soluciones de servicios gestionados e integrados de telecomunicaciones dirigido al sector mype es una propuesta viable desde el an?lisis t?cnico y econ?mico. La propuesta destaca tres factores: En primer lugar, un mercado atractivo en tama?o, en Lima Centro, a diciembre 2015 exist?an 373,670 mypes, de los cuales el 95.85% de las unidades registradas son micro empresas y el 4.15% peque?as empresas (INEI, Estrutura empresarial, 2015). En segundo lugar, una demanda no satisfecha; necesidades no cubiertas en los servicios de telecomunicaciones para mypes (la oferta de los proveedores dirigida a las grandes empresas y los precios son las causas m?s comunes de este problema). Finalmente, el conocimiento del rubro de los integrantes del grupo y una nueva visi?n de gesti?n hacen que la propuesta de valor de la empresa radique en bridar servicios gestionados que se ajusten a las necesidades del cliente

Detectability of Plasmodium falciparum clones

BACKGROUND: In areas of high transmission people often harbour multiple clones of Plasmodium falciparum, but even PCR-based diagnostic methods can only detect a fraction (the detectability, q) of all clones present in a host. Accurate measurements of detectability are desirable since it affects estimates of multiplicity of infection, prevalence, and frequency of breakthrough infections in clinical drug trials. Detectability can be estimated by typing repeated samples from the same host but it has been unclear what should be the time interval between the samples and how the data should be analysed. METHODS: A longitudinal molecular study was conducted in the Kassena-Nankana district in northern Ghana. From each of the 80 participants, four finger prick samples were collected over a period of 8 days, and tested for presence of different Merozoite Surface Protein (msp) 2 genotypes. Implications for estimating q were derived from these data by comparing the fit of statistical models of serial dependence and over-dispersion. RESULTS: The distribution of the frequencies of detection for msp2 genotypes was close to binomial if the time span between consecutive blood samples was at least 7 days. For shorter intervals the probabilities of detection were positively correlated, i.e. the shorter the interval between two blood collections, the more likely the diagnostic results matched for a particular genotype. Estimates of q were rather insensitive to the statistical model fitted. CONCLUSIONS: A simple algorithm based on analysing blood samples collected 7 days apart is justified for generating robust estimates of detectability. The finding of positive correlation of detection probabilities for short time intervals argues against imperfect detection being directly linked to the 48-hour periodicity of P. falciparum. The results suggest that the detectability of a given parasite clone changes over time, at an unknown rate, but fast enough to regard blood samples taken one week apart as statistically independent

Microservice Transition and its Granularity Problem: A Systematic Mapping Study

Microservices have gained wide recognition and acceptance in software industries as an emerging architectural style for autonomic, scalable, and more reliable computing. The transition to microservices has been highly motivated by the need for better alignment of technical design decisions with improving value potentials of architectures. Despite microservices' popularity, research still lacks disciplined understanding of transition and consensus on the principles and activities underlying "micro-ing" architectures. In this paper, we report on a systematic mapping study that consolidates various views, approaches and activities that commonly assist in the transition to microservices. The study aims to provide a better understanding of the transition; it also contributes a working definition of the transition and technical activities underlying it. We term the transition and technical activities leading to microservice architectures as microservitization. We then shed light on a fundamental problem of microservitization: microservice granularity and reasoning about its adaptation as first-class entities. This study reviews state-of-the-art and -practice related to reasoning about microservice granularity; it reviews modelling approaches, aspects considered, guidelines and processes used to reason about microservice granularity. This study identifies opportunities for future research and development related to reasoning about microservice granularity.Comment: 36 pages including references, 6 figures, and 3 table