Observations of one young and three middle-aged γ\gamma-ray pulsars with the Gran Telescopio Canarias

We used the 10.4m Gran Telescopio Canarias to search for the optical counterparts to four isolated $\gamma$-ray pulsars, all detected in the X-rays by either \xmm\ or \chan\ but not yet in the optical. Three of them are middle-aged pulsars -- PSR\, J1846+0919 (0.36 Myr), PSR\, J2055+2539 (1.2 Myr), PSR\, J2043+2740 (1.2 Myr) -- and one, PSR\, J1907+0602, is a young pulsar (19.5 kyr). For both PSR\, J1907+0602 and PSR\, J2055+2539 we found one object close to the pulsar position. However, in both cases such an object cannot be a viable candidate counterpart to the pulsar. For PSR\, J1907+0602, because it would imply an anomalously red spectrum for the pulsar and for PSR\, J2055+2539 because the pulsar would be unrealistically bright ($r'=20.34\pm0.04$) for the assumed distance and interstellar extinction. For PSR\, J1846+0919, we found no object sufficiently close to the expected position to claim a possible association, whereas for PSR\, J2043+2740 we confirm our previous findings that the object nearest to the pulsar position is an unrelated field star. We used our brightness limits ($g' \approx 27$), the first obtained with a large-aperture telescope for both PSR\, J1846+0919 and PSR\, J2055+2539, to constrain the optical emission properties of these pulsars and investigate the presence of spectral turnovers at low energies in their multi-wavelength spectra.Comment: 10 pages, 11 figures, accpted for publication in MNRA

Unsteady flow regimes in arrow-shaped micro-mixers with different tilting angles

Two arrow-shaped micro-mixers, obtained from the classical T-shaped geometry by tilting downward the inlet channels, are considered herein. The two configurations, having different tilting angle values, have been chosen since they show significantly different flow topologies and mixing performances at low Reynolds numbers. In the present paper, we use both experimental flow visualizations and direct numerical simulations to shed light on the mixing behavior of the two configurations for larger Reynolds numbers, for which the mixers present unsteady periodic flows, although in laminar flow conditions. The tilting angle influences the flow dynamics also in the unsteady regimes and has a significant impact on mixing. The configuration characterized by the lower tilting angle, i.e., α = 10°, ensures a better global mixing performance than the one with the larger angle, i.e., α = 20°

Mixing sensitivity to the inclination of the lateral walls in a T-mixer

One of the simplest geometries for micro-mixers has a T-shape, i.e., the two inlets join perpendicularly the mixing channel. The cross-sections of the channels are usually square/rectangular, as straight walls facilitate experimental and modeling analysis. On the contrary, this work investigates through Computational Fluid Dynamics the effect of a cross-section with lateral walls inclined of an angle α as such an inclination may stem from different microfabrication techniques. Considering water as operating fluid, the same mixing performance as square/rectangular cross-sections is obtained for inclinations α≤3°; this indicates the maximum admissible error on the perpendicularity of the walls in the manufacturing process. Above this value, the presence of inclined walls delays the onset of the engulfment regime at higher Reynolds numbers, and for α≥23°the mixing is hampered dramatically, as the flow is unable to break the mirror symmetry and enter in the engulfment regime. At low Reynolds numbers, the mixing is moderately improved for α≥10°, because the vortex regime presents a lower degree of symmetry than that of T-mixers with straight walls

Statistical properties of an ideal subgrid-scale correction for Lagrangian particle tracking in turbulent channel flow

One issue associated with the use of Large-Eddy Simulation (LES) to investigate the dispersion of small inertial particles in turbulent flows is the accuracy with which particle statistics and concentration can be reproduced. The motion of particles in LES fields may differ significantly from that observed in experiments or direct numerical simulation (DNS) because the force acting on the particles is not accurately estimated, due to the availability of the only filtered fluid velocity, and because errors accumulate in time leading to a progressive divergence of the trajectories. This may lead to different degrees of inaccuracy in the prediction of statistics and concentration. We identify herein an ideal subgrid correction of the a-priori LES fluid velocity seen by the particles in turbulent channel flow. This correction is computed by imposing that the trajectories of individual particles moving in filtered DNS fields exactly coincide with the particle trajectories in a DNS. In this way the errors introduced by filtering into the particle motion equations can be singled out and analyzed separately from those due to the progressive divergence of the trajectories. The subgrid correction term, and therefore the filtering error, is characterized in the present paper in terms of statistical moments. The effects of the particle inertia and of the filter type and width on the properties of the correction term are investigated.Comment: 15 pages,24 figures. Submitted to Journal of Physics: Conference Serie

Effect of stratification on the mixing and reaction yield in a T-shaped micro-mixer

The effect of a small density difference, i.e., lower than 12%, between the two miscible liquid streams fed to a T-shaped junction is investigated experimentally and through numerical simulations. Micron-resolution particle image velocimetry (micro-PIV) experiments provided detailed support to the numerical analysis of how stratification influences flow features in different flow regimes. From dimensional analysis, we find that gravitational and inertial fluxes balance each other at a distance L=d/Ri from the confluence along the mixing channel, where d is the hydraulic diameter and Ri is the Richardson number. In general, at distances |y|≪L, the influence of gravity can be neglected, while at |y|≫L the two fluids are fully segregated; in particular, at the confluence, the flow field is the same as the one that we obtain assuming that the two inlet fluids are identical. Thus, in the segregated regime, the contact region separating the two fluids of the inlet streams remains vertical at distances |y|≪L along the mixing channel while it becomes progressively horizontal at |y|≈L. In the vortex regime as well, near the confluence the flow field presents a mirror symmetry, with a very small resulting degree of mixing; however, as we move down the mixing channel, when |y|>L, gravity becomes relevant, leading to a symmetry breaking that promotes convection and enhances mixing. When we further increase the Reynolds number, in the engulfment regime, the degree of mixing becomes much larger due to the mixing induced by the flow instability at the confluence and thus the successive stratification appears to have a small effect on the flow topology, with a degree of mixing that continues to grow very slowly in the mixing channel, similar to what happens in the case of identical inlet fluids. As expected, the onsets of the vortex and engulfment regimes occur at values of the Reynolds number Re that hardly depend on the density difference between the two inlet fluids, provided that Re is defined in terms of the fluid properties of a homogeneous fluid mixture. Finally, the reaction yield along the mixing channel is computed both from numerical and experimental data. In agreement with theoretical predictions, we found that the reaction yield depends on the Damköhler number and the kinetic constant, while it is independent of the density ratio, at least within the range of the investigated conditions

Suitability of the nisin Z-producer lactococcus lactis subsp. lactis CBM 21 to be used as an adjunct culture for squacquerone cheese production

This research investigated the technological and safety effects of the nisin Z producer Lactococcus lactis subsp. lactis CBM 21, tested as an adjunct culture for the making of Squacquerone cheese in a pilot-scale plant. The biocontrol agent remained at a high level throughout the cheese refrigerated storage, without having a negative influence on the viability of the conventional Streptococcus thermophilus starter. The inclusion of CBM 21 in Squacquerone cheesemaking proved to be more effective compared to the traditional one, to reduce total coliforms and Pseudomonas spp. Moreover, the novel/innovative adjunct culture tested did not negatively modify the proteolytic patterns of Squacquerone cheese, but it gave rise to products with specific volatile and texture profiles. The cheese produced with CBM 21 was more appreciated by the panelists with respect to the traditional one

On the performance of a cointegration-based approach for novelty detection in realistic fatigue crack growth scenarios

Confounding influences, such as operational and environmental variations, represent a limitation to the implementation of Structural Health Monitoring (SHM) systems in real structures, potentially leading to damage misclassifications. In this framework, this study considers cointegration as a state of the art method for data normalisation in fatigue crack propagation scenarios, where monitoring is performed by a distributed network of strain sensors. Specifically, the work is aimed at demonstrating the effectiveness of cointegration on real engineering data in a new context, where the damage is continuously growing. Cointegration is applied at first in a controlled scenario consisting of a numerical strain simulation by means of a finite element model, modified in order to take realistic temperature fluctuations and sensor noise into account. Afterwards, detrending and anomaly detection performances are verified in two different experimental programmes on realistic aeronautical structures subjected to fatigue crack growth, including a full-scale fatigue test on a helicopter tail boom. Strain measurements are taken from a network of Fibre Bragg Grating (FBG) sensors, known to be extremely sensitive to temperature variations, hence delivering challenging scenarios for cointegration testing. Results are shown to be in good agreement with the experimental evidence, with the cointegration algorithm capable of detecting the onset of damage propagation within a 4 mm increment from a baseline condition

FlhF, a signal recognition particle-like GTPase, is involved in the regulation of flagellar arrangement, motility behaviour and protein secretion in Bacillus cereus

Flagellar arrangement is a highly conserved feature within bacterial species. However, only a few genes regulating cell flagellation have been described in polar flagellate bacteria. This report demonstrates that the arrangement of flagella in the peritrichous flagellate Bacillus cereus is controlled by flhF. Disruption of flhF in B. cereus led to a reduction in the number of flagella from 10-12 to 1-3 filaments per cell in the insertion mutant MP06. Moreover, compared to the parental strain, MP06 exhibited: (i) shorter smooth swimming phases, causing reduced swimming motility but not affecting chemotaxis; (ii) complete inhibition of swarming motility, as differentiated swarm cells were never detected; (iii) an increased amount of extracellular proteins; and (iv) differential export of virulence determinants, such as haemolysin BL (HBL), phosphatidylcholine-preferring phospholipase C (PC-PLC) and non-haemolytic enterotoxin (NHE). Introduction of a plasmid harbouring flhF (pDGflhF) into MP06 completely restored the wild-type phenotype in the trans-complemented strain MP07. B. cereus flhF was found to constitute a monocistronic transcriptional unit and its overexpression did not produce abnormal features in the wild-type background. Characterization of a B. cereus mutant (MP05) carrying a partial flhF deletion indicated that the last C-terminal domain of FlhF is involved in protein export while not required for flagellar arrangement and motility behaviour. Taken together, these data suggest that B. cereus FlhF is a promising candidate for connecting diverse cellular functions, such as flagellar arrangement, motility behaviour, pattern of protein secretion and virulence phenotype

Alguns aspectos relacionados a resposta ovariana de vacas superovuladas dos grupos genéticos Canchim e Nelore (resultados preliminares).

Estudou-se a resposta a superovulação com diferentes dosagens de FSH-Pe prostaglandina em doadoras dos grupos geneticos Canchim e Nelore