Was the English medieval goat genuinely rare? A new morphometric approach provides the answer

There are important gaps in the historical and archaeological evidence that have, so far, precluded us from reliably assessing the role of the goat in the English Middle Ages. This, in part, is the direct consequence of the absence of a methodology allowing the confident identification of sheep and goat bones. On the other hand, the fact that the goat has always been perceived as rare has led us to think that medieval goats did not deserve much attention. Thanks to a recently developed new morphometric approach, which allows taxonomic identifications to be based on more objective criteria and results to be scrutinised, we are provided with a new tool to re-assess the role that this species played in English medieval husbandry. This paper presents the results of the application of this new methodology on three archaeological medieval sheep/goat assemblages. Previous research suggested that the goat was not abundant in medieval England, but has also raised the possibility that this may be a consequence of an under-estimation by zooarchaeologists, due to identification difficulties. The basic outcome of our paper is to provide, for the first time, unambiguous evidence that the goat was genuinely uncommon. In the medieval archaeological record, sheep remains are overwhelmingly better represented than goat remains—all three case studies confirmed the pattern. Although these examples cannot be taken to represent the situation everywhere in the country, they provide clear-cut indication that the zoorchaeological interpretation of caprine remains from English medieval sites has so far been largely reliable. The three sites offer the opportunity to investigate different dimensions of the problem and to discuss the role of the goat in different contexts

Anti-biofilm inhibitory synergistic effects of combinations of essential oils and antibiotics

In recent years, the increase of bacteria antibiotic-resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According to in vitro studies, EOs and their components could show such effects. Among them, we studied the activity of Cinnammonum zeylanicum, Mentha piperita, Origanum vulgare, and Thymus vulgaris EOs on bacterial biofilm and their synergism when used in association with some common antibiotics such as norfloxacin, oxacillin, and gentamicin. The chemical composition of EOs was determined using gas chromatography (GC) coupled with mass spectrometry (MS) techniques. The EOs drug efficacy was evaluated on four different strains of Gram-positive bacteria forming biofilms. The synergistic effects were tested through the chequerboard microdilution method. The association EOs-antibiotics showed a strong destruction of the biofilm growth of the four bacterial species considered. The interaction of norfloxacin with EOs was the most effective in all the tested combinations against the strains object of this study. These preliminary results suggest the formulation of a new generation of antimicrobial agents based on a combination of antimicrobial compounds with different origin

Building and tanning in the 18th and 19th centuries: an analysis of cattle horncores from Greenwich High Road (London)

The assemblage from Greenwich High Road has interesting implications for our understanding of 18th and early 19th century tanneries and also of the use of bones as building material. The study of the age at death of the animals revealed that the horncores are mostly from fully adult individuals, probably culled draught animals. This hypothesis is supported by biometrical analysis. The comparison of the Greenwich horncores with modern data and archaeological evidence suggests that the Greenwich assemblage is predominantly composed of oxen. The horncores were used to line a drain on the site and are likely to represent waste material resulting from the activities that took place in the nearby tannery. Skins and horns were removed for craft purposes while the bony cores, with no economic value, were simply used as cheap and ready material to help the construction of the drain

Polyphenols from Olive-Mill Wastewater and Biological Activity: Focus on Irritable Bowel Syndrome

Waste represents a cost for companies, in particular for agro-food companies, which can become a resource as a secondary material. In this work, we examine three products of olive-oil waste water, named MOMAST® (Plus30, PW25, and HY100). Based on the chemical composition, obtained with different methods, we hypothesized a possible application as food supplements in irritable bowel syndrome (IBS). We therefore studied MOMASTs on some targets linked to this pathology: antioxidant action and spontaneous and induced intestinal contractility of the ileum and colon. Plus30, which showed a more promising biological of activity also for its oleuropein content, was characterized by an interesting action against some microorganisms. The results highlighted the ability of Plus30 to modulate spontaneous and induced contractility, to exert a good antioxidant action, and to significantly act on various microorganisms. These effects are synergistic in the presence of antibiotics. In conclusion, we can confirm that Plus30 could be a great candidate as a food supplement in patients with IBS

Synergistic activity of new diclofenac and essential oils combinations against different candida spp

According to recent studies, Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) have shown a good antimicrobial and antifungal activity. Their association with essential oils (EOs) could be useful for the treatment of infections caused by Candida spp. The aim of this studyis to evaluate the synergistic antifungal activity of new combinations between Diclofenac Sodium Salt (DSS), a widely used NSAID, with EOs of Mentha × piperita, Pelargonium graveolens and Melaleuca alternifolia. The in-vitro antifungal activity was determined on different Candida strains. The determination of the chemical composition of EOs was carried out by gaschromatography-massspectrometry (GC-MS). Susceptibility testing of planktonic cells was performed by using the broth microdilution assay and checkerboard methods. Minimum Inhibitory Concentrations (MIC) of DSS was in a range from 1.02 to 2.05 µg/mL reaching a MIC value of 0.05 µg/mL when combined with Pelargonium graveolens (FICI = 0.23–0.35) or Menthapiperita (FICI = 0.22–0.30) EOs. These preliminary results show thatthe combination of the EOs with DSS improves the antifungal activity on all the tested Candida strains

A method to assess wear rate in pig teeth from archaeological sites

The recording of age at death is an important aspect of zooarchaeological analysis as it provides evidence about a variety of research questions, spanning from the origins of domestication to husbandry strategies. Age estimation based on tooth eruption and wear is a commonly used method to establish the age at death of archaeological populations. However, this approach has its limitations. It relies on the principle that tooth wear rate is relatively constant in different populations but, since no method has ever been developed to quantify the rate of wear, such an assumption has never been fully verified. As a consequence, the extent to which variable speeds of wear in different populations may affect age estimations is still unknown. To clarify this bias and offer transparency into the issue, the development of a method to assess wear rate in archaeological teeth is of paramount importance. In this paper, we propose a simple system that allows such an assessment to be undertaken. The system has been developed for pig mandibular/lower teeth but can also be extended to other species. The methodology is then tested on several English Late Medieval and Early Modern pig assemblages which represent ideal case studies as they cover a historical period when extensive changes in pig dietary regimes occurred. The evidence reassuringly suggests that differences in wear rates between these periods were not substantial, which bodes well for the comparability of kill-off patterns. However, comparisons with several outgroups indicate that the potential range of wear rates is much greater than attested in our core case study. Wild boars and prehistoric pigs, in particular, appear to wear their molars more slowly. Caution is therefore needed and it is suggested that tooth wear rates (TWR) and average wear rates (AWR) should routinely be calculated when tooth-based age profiles are analysed

Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity

Lack of physical exercise is considered an important risk factor for chronic diseases. On the contrary, physical exercise reduces the morbidity rates of obesity, diabetes, bone disease, and hypertension. In order to gain novel molecular and cellular clues, we analyzed the effects of physical exercise on differentiation of mesenchymal circulating progenitor cells (M-CPCs) obtained from runners. We also investigated autophagy and telomerase-related gene expression to evaluate the involvement of specific cellular functions in the differentiation process. We performed cellular and molecular analyses in M-CPCs, obtained by a depletion method, of 22 subjects before (PRE RUN) and after (POST RUN) a half marathon performance. In order to prove our findings, we performed also in vitro analyses by testing the effects of runners' sera on a human bone marrow-derived mesenchymal stem (hBM-MSC) cell line. PCR array analyses of PRE RUN versus POST RUN M-CPC total RNAs put in evidence several genes which appeared to be modulated by physical activity. Our results showed that physical exercise promotes differentiation. Osteogenesis-related genes as RUNX2, MSX1, and SPP1 appeared to be upregulated after the run; data showed also increased levels of BMP2 and BMP6 expressions. SOX9, COL2A1, and COMP gene enhanced expression suggested the induction of chondrocytic differentiation as well. The expression of telomerase-associated genes and of two autophagy-related genes, ATG3 and ULK1, was also affected and correlated positively with MSC differentiation. These data highlight an attractive cellular scenario, outlining the role of autophagic response to physical exercise and suggesting new insights into the benefits of physical exercise in counteracting chronic degenerative conditions

Molecular simplification of natural products: Synthesis, antibacterial activity, and molecular docking studies of berberine open models

Berberine, the main bioactive component of many medicinal plants belonging to various genera such as Berberis, Coptis, and Hydrastis is a multifunctional compound. Among the numerous interesting biological properties of berberine is broad antimicrobial activity including a range of Gram-positive and Gram-negative bacteria. With the aim of identifying berberine analogues possibly endowed with higher lead-likeness and easier synthetic access, the molecular simplification approach was applied to the secondary metabolite and a series of analogues were prepared and screened for their antimicrobial activity against Gram-positive and Gram-negative bacterial test species. Rewardingly, the berberine simplified analogues displayed 2–20-fold higher potency with respect to berberine. Since our berberine simplified analogues may be easily synthesized and are characterized by lower molecular weight than the parent compound, they are further functionalizable and should be more suitable for oral administration. Molecular docking simulations suggested FtsZ, a well-known protein involved in bacterial cell division, as a possible target