606 research outputs found
Rapid on-Chip Assembly of Niosomes: Batch versus Continuous Flow Reactors
The large-scale continuous production of niosomes remains challenging. The inherent drawbacks of batch processes such as large particle polydispersity and reduced batch-to-batch reproducibility are here overcome by using commercially available microfluidic reactors. Compared to the traditional batch-based film hydration method, herein, we demonstrate that it is possible to carry out the homogeneous, large-scale (up to 120 mg/min) production of niosomes using two different synthesis techniques (the thin film hydration method and the emulsification technique). Niosomes particle size can be controlled depending on the need by varying the synthesis temperature. The high cytocompatibility of the resulting niosomes was also demonstrated in this work on three different somatic cell lines. For the first time, the structure of the niosome multilamellar shell was also elucidated using high-resolution transmission electron microscopy (HR-STEM) as well as their colloidal stability over time (6 weeks) under different storage conditions. The morphology of cryo-protected or as-made niosomes was also evaluated by HR-STEM after freeze-drying. Finally, the dual ability of those synthetic, nonionic, surfactant-based vesicles to carry both hydrophilic and hydrophobic molecules was also here demonstrated by using laser scanning confocal microscopy
Analysis of backgrounds for the ANAIS-112 dark matter experiment
The ANAIS (Annual modulation with NaI(Tl) Scintillators) experiment aims at
the confirmation or refutation of theDAMA/LIBRA positive annual modulation
signal in the low energy detection rate, using the same target and technique,
at the Canfranc Underground Laboratory (LSC) in Spain. ANAIS-112, consisting of
nine 12.5 kg NaI(Tl) modules produced by Alpha Spectra Inc. in a 3x3matrix
configuration, is taking data smoothly in "dark matter search" mode since
August, 2017, after a commissioning phase and operation of the first detectors
during the last years in various setups. A large effort has been carried out
withinANAIS to characterize the background of sodium iodide detectors, before
unblinding the data and performing the first annual modulation analysis. Here,
the background models developed for all the nine ANAIS-112 detectors are
presented. Measured spectra from threshold to high energy in different
conditions are well described by the models based on quantified activities
independently estimated following several approaches. In the region from 1 to 6
keVee the measured, efficiency corrected background level is 3.58+-0.02 keV-1
kg-1 day-1; NaI crystal bulk contamination is the dominant background source
being 210Pb, 40K, 22Na and 3H contributions the most relevant ones. This
background level, added to the achieved 1 keVee analysis threshold (thanks to
the outstanding light collection and robust filtering procedures developed),
allow ANAIS-112 to be sensitive to the modulation amplitude measured by
DAMA/LIBRA, and able to explore at three sigma level in 5 years the WIMP
parameter region singled out by this experiment.Comment: Final version for publicatio
Evidences of the Cerium Oxide-Catalysed DPF Regeneration in a Real Diesel Engine Exhaust
The active phase Ce0.5Pr0.5O2 has been loaded on commercial substrates (SiC DPF and cordierite honeycomb monolith) to perform DPF regeneration experiments in the exhaust of a diesel engine. Also, a powder sample has been prepared to carry out soot combustion experiments at laboratory. Experiments performed in the real diesel exhaust demonstrated the catalytic activity of the Ce–Pr mixed oxide for the combustion of soot, lowering the DPF regeneration temperature with regard to a counterpart catalyst-free DPF. The temperature for active regeneration of the Ce0.5Pr0.5O2-containing DPF when the soot content is low is in the range of 500–550 °C. When the Ce0.5Pr0.5O2-containing DPF is saturated with a high amount of soot, pressure drop and soot load at the filter reach equilibrium at around 360 °C under steady state engine operation due to passive regeneration. The uncoated DPF reached this equilibrium at around 440 °C. Comparing results at real exhaust with those at laboratory allow concluding that the Ce0.5Pr0.5O2-catalysed soot combustion in the real exhaust is not based on the NO2-assisted mechanism but is most likely occurring by the active oxygen-based mechanism.The authors thank the financial support of Generalitat Valenciana (Project Prometeo 2009/047), Spanish Ministry of Science and Innovation (project CIT-420000-2009-48) and EU (FEDER funding)
Retinal nerve fiber layer atrophy is associated with physical and cognitive disability in multiple sclerosis
Studying axonal loss in the retina is a promising biomarker for
multiple sclerosis (MS). Our aim was to compare optical coherence tomography
(OCT) and Heidelberg retinal tomography (HRT) techniques to measure the thickness
of the retinal nerve fiber layer (RNFL) in patients with MS, and to explore the
relationship between changes in the RNFL thickness with physical and cognitive
disability. We studied 52 patients with MS and 18 proportionally matched controls
by performing neurological examination, neuropsychological evaluation using the
Brief Repetitive Battery-Neuropsychology and RNFL thickness measurement using OCT
and HRT. RESULTS: We found that both OCT and HRT could define a reduction in the
thickness of the RNFL in patients with MS compared with controls, although both
measurements were weakly correlated, suggesting that they might measure different
aspects of the tissue changes in MS. The degree of RNFL atrophy was correlated
with cognitive disability, mainly with the symbol digit modality test (r=0.754,
P<0.001). Moreover, temporal quadrant RNFL atrophy measured with OCT was
associated with physical disability. CONCLUSION: In summary, both OCT and HRT are
able to detect thinning of the RNFL, but OCT seems to be the most sensitive
technique to identify changes associated with MS evolution
SPL8 and miR156-targeted SPL genes redundantly regulate Arabidopsis gynoecium differential patterning
Valproic acid inhibits interferon-γ production by NK cells and increases susceptibility to <i>Listeria monocytogenes</i> infection
Effects of lutein and docosahexaenoic Acid supplementation on macular pigment optical density in a randomized controlled trial
We studied the macular pigment ocular density (MPOD) in patients with early age macular degeneration (AMD) before and 1 year after nutritional supplementation with lutein and docosahexaenoic acid (DHA). Forty-four patients with AMD were randomly divided into two groups that received placebo (n = 21) or a nutritional supplement (n = 23, 12 mg of lutein and 280 mg of DHA daily). Heterochromatic flicker photometry was used to determine the MPOD. At baseline, the MPOD in AMD patients with placebo was 0.286 ± 0.017 meanwhile in AMD patients with supplementation it was 0.291 ± 0.016. One year later, the mean MPOD had increased by 0.059 in the placebo group and by 0.162 in patients receiving lutein and DHA. This difference between groups was significant (p < 0.05). Lutein and DHA supplementation is effective in increasing the MPOD and may aid in prevention of age related macular degeneration
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