Laminosioptes cysticola and Gallibacterium anatis infections in a lymphoma diseased chicken hen with a cystic right oviduct

A domestic hen showing infraorbital swelling was presented for a routine classroom demonstration of avian diagnostics. At necropsy, tiny whitish caseo-calcareous nodules were found in the subcutaneous tissues of the carcass, produced by the subcutaneous fowl mite, Laminosioptes cysticola. Gallibacterium anatis biovar haemolytica was isolated from the infraorbital sinus, it containing a caseous exudate. During necropsy, a conspicuous cyst was found in the abdominal cavity. Microscopic examination of the internal lining of the cyst revealed a single cuboidal to columnar, ciliated epithelium, leading to a diagnosis of oviductal cyst. Also, the microscopic examination of the heart, lung, liver and kidney reveal a multifocal infiltration of lymphoma cells. It appears the first case of simultaneous presentation of these conditions from a single chicken

Methodology for optimization of distributed biomass resources evaluation, management and final energy use

[EN] A methodology has been developed to assess optimal management and energy use of distributed biomass resources, where logistics is a main factor but other features must be also considered: biomass resources properties (quantity, quality, seasonality & availability), plant size effect, available technologies for power, heat and solid biofuels generation, CO2 emissions balance and quantification of potential biofuel consumers. This methodology provides a quantification and characterization of biomass resources, a list of optimal locations from logistic point of view and the necessary data to perform detailed technical, economic and environmental analysis of the different biomass energy use options. It has been applied to three districts of the Valencian region in Spain and main results and conclusions are also included in this paper.This work was completed in the framework of the activities of the biomass research group of the IIE (Instituto de Ingenieria Energetica) in regional, national and international. The authors deeply thank all the organizations involved in these projects for their support and, specially, IMPIVA-Generalitat Valenciana, the Spanish Ministry of Science and Technology and the European Commission for the projects BIOVAL, BIODER and EU-DEEP respectively, for the provided funding that made this work possible.Alfonso-Solar, D.; Perpiñá, C.; Pérez-Navarro, A.; Peñalvo-López, E.; Vargas-Salgado, C.; Cárdenas, R. (2009). Methodology for optimization of distributed biomass resources evaluation, management and final energy use. Biomass and Bioenergy. 33(8):1070-1079. https://doi.org/10.1016/j.biombioe.2009.04.0021070107933

Solution to the Reliability Problem of Radial Electric Distribution Systems Through Artificial Intelligence

In this paper the distribution electric system reliability is recognized like an artificial intelligence problem. How this idea is applied in evaluation of reliability is detailed. Concepts as Intelligence Matrix and Inter-feeder Route are defined. From the last one a reliability prediction strategy for medium voltage networks is proposed and tested

Sensitive quantification of the HIV-1 reservoir in gut-associated lymphoid tissue

Background: The implementation of successful strategies to achieve an HIV cure has become a priority in HIV research. However, the current location and size of HIV reservoirs is still unknown since there are limited tools to evaluate HIV latency in viral sanctuaries such as gut-associated lymphoid tissue (GALT). As reported in the so called "Boston Patients", despite undetectable levels of proviral HIV-1 DNA in blood and GALT, viral rebound happens in just few months after ART interruption. This fact might imply that current methods are not sensitive enough to detect residual reservoirs. Showing that, it is imperative to improve the detection and quantification of HIV-1 reservoir in tissue samples. Herein, we propose a novel non-enzymatic protocol for purification of Lamina Propria Leukocytes (LPL) from gut biopsies combined to viral HIV DNA (vDNA) quantification by droplet digital PCR (ddPCR) to improve the sensitivity and accuracy of viral reservoir measurements (LPL-vDNA assay). Methods: Endoscopic ileum biopsies were sampled from 12 HIV-1-infected cART-suppressed subjects. We performed a DTT/EDTA-based treatment for epithelial layer removal followed by non-enzymatic disruption of the tissue to obtain lamina propria cell suspension (LP). CD45+ cells were subsequently purified by flow sorting and vDNA was determined by ddPCR. Results: vDNA quantification levels were significantly higher in purified LPLs (CD45+) than in bulk LPs (p<0.01). The levels of vDNA were higher in ileum samples than in concurrent PBMC from the same individuals (p = 0.002). As a result of the increased sensitivity of this purification method, the Poisson 95% confidence intervals of the vDNA quantification data from LPLs were narrower than that from bulk LPs. Of note, vDNA was unambiguously quantified above the detection limit in 100% of LPL samples, while only in 58% of bulk LPs. Conclusion: We propose an innovative combined protocol for a more sensitive detection of the HIV reservoir in gut-associated viral sanctuaries, which might be used to evaluate any proposed eradication strategy

Economic activity and congenital anomalies: An ecologic study in Argentina

In this study, we analyze the association between industrial activity and the occurrence of 34 congenital anomalies. We selected 21 counties in Argentina during 1982-1994 and examined a total of 614,796 births in these counties in consecutive series. We used the International Standard Industrial Classification of All Economic Activities as an indicator of exposure to 80 specific industrial activities. Incidence rate ratios for each congenital anomaly were adjusted by the socioeconomic level of the county according to a census index of social deprivation. For a given exposure/anomaly association to be considered as significant and relevant, the exposure had to be a statistically significant risk for the occurrence of the anomaly and an increase in the birth prevalence rate of the congenital anomaly type involved had to be observed in those counties where the putative causal activity was being performed. Significant associations (p < 0.01) were identified between textile industry and anencephaly, and between the manufacture of engines and turbines and microcephaly. These observations are consistent with previous reports on occupational exposure, and their further investigation by means of case-control studies is recommended.Instituto Multidisciplinario de Biología Celula

Economic activity and congenital anomalies: An ecologic study in Argentina

In this study, we analyze the association between industrial activity and the occurrence of 34 congenital anomalies. We selected 21 counties in Argentina during 1982-1994 and examined a total of 614,796 births in these counties in consecutive series. We used the International Standard Industrial Classification of All Economic Activities as an indicator of exposure to 80 specific industrial activities. Incidence rate ratios for each congenital anomaly were adjusted by the socioeconomic level of the county according to a census index of social deprivation. For a given exposure/anomaly association to be considered as significant and relevant, the exposure had to be a statistically significant risk for the occurrence of the anomaly and an increase in the birth prevalence rate of the congenital anomaly type involved had to be observed in those counties where the putative causal activity was being performed. Significant associations (p < 0.01) were identified between textile industry and anencephaly, and between the manufacture of engines and turbines and microcephaly. These observations are consistent with previous reports on occupational exposure, and their further investigation by means of case-control studies is recommended.Instituto Multidisciplinario de Biología Celula

Nuclear Structure Functions at Small x from Inelastic Shadowing and Diffraction

Nuclear structure functions at small x and small or moderate $Q^2$ are studied using the relation with diffraction on nucleons which arises from Gribov's Reggeon Calculus. A reasonable description of experimental data is obtained with no fitted parameters. A comparison with other models and predictions for future lepton-ion colliders are provided. Consequences for the reduction of multiplicities in nucleus-nucleus collisions at energies of RHIC and LHC are examined.Comment: LaTeX, 32 pages, 14 eps figures included using epsfig; 1 reference update