Comparison of Electrophoretic Protein Profiles from Sheep and Goat Parotid Saliva

Saliva provides a medium for short-term adaptation to changes in diet composition, namely, the presence of plant secondary metabolites. Salivary proteins have biological functions that have particular influence on oral homeostasis, taste, and digestive function. Some salivary proteins, such as proline-rich proteins, are present in browsers but absent in grazers. Despite the significance of salivary proteins, their expression patterns in many herbivores are unknown. We investigated the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile of parotid salivary proteins from two domesticated species, one a grazer, the sheep, Ovis aries, and the other a mixed feeder, the goat, Capra hircus, both fed on the same conventional diet. With 12.5% polyacrylamide linear gels, we observed uniform patterns of salivary proteins within the two species. In the goat profile, 21 major bands were observed, and 19 in the sheep profile. Each band was subjected to peptide mass fingerprinting for purposes of identification, allowing for 16 successful protein identifications. Marked differences were observed between the species in the region of 25–35 kDa molecular weights: one band was present in significantly different intensities; three bands were present only in goats; and one band was present only in sheep. This is the first report of a comparison of the protein salivary composition of sheep and goats and suggests that future research should be conducted to reveal a physiological function for salivary proteins related to the differences in feeding behavior of these species

Current in the light-front Bethe-Salpeter formalism II: Applications

We pursue applications of the light-front reduction of current matrix elements in the Bethe-Salpeter formalism. The normalization of the reduced wave function is derived from the covariant framework and related to non-valence probabilities using familiar Fock space projection operators. Using a simple model, we obtain expressions for generalized parton distributions that are continuous. The non-vanishing of these distributions at the crossover between kinematic regimes (where the plus component of the struck quark's momentum is equal to the plus component of the momentum transfer) is tied to higher Fock components. Moreover continuity holds due to relations between Fock components at vanishing plus momentum. Lastly we apply the light-front reduction to time-like form factors and derive expressions for the generalized distribution amplitudes in this model.Comment: 12 pages, 6 figures, RevTex

Multipurpose underwater manipulation for archaeological intervention

This paper presents a new research line recently started by our team in the context of semi-autonomous underwater robotic intervention applied to the archaeological domain. This new research line is supported by the Spanish Ministry and represents an extension of previous developed work through different national and international projects. The robotic system under development will assist the archaeologist in the detailed work of monitoring, characterization, study, reconstruction and preservation of archaeological sites, always in accordance with the continuous supervision of the human expert.Peer Reviewe

Magnetic field chaos in the SK Model

We study the Sherrington--Kirkpatrick model, both above and below the De Almeida Thouless line, by using a modified version of the Parallel Tempering algorithm in which the system is allowed to move between different values of the magnetic field h. The behavior of the probability distribution of the overlap between two replicas at different values of the magnetic field h_0 and h_1 gives clear evidence for the presence of magnetic field chaos already for moderate system sizes, in contrast to the case of temperature chaos, which is not visible on system sizes that can currently be thermalized.Comment: Latex, 16 pages including 20 postscript figure

Kinin B1 receptor gene ablation affects hypothalamic CART production

A role for the kinin B1 receptor in energy-homeostatic processes was implicated by previous works. Notably the studies where kinin B1 receptor knockout mice (B1-/-) are observed to have impaired adiposity, impaired leptin and insulin production, lower feed efficiency, protection from liver steatosis and diet induced obesity when fed a high fat diet (HFD). More particularly, in a model where the B1 receptor is expressed exclusively in the adipose tissue, it rescues the plasma insulin concentration and the weight gain seen in wild type mice. Taking into consideration that leptin participates in the formation of hypothalamic nuclei, which modulate energy expenditure, and feeding behavior, we hypothesized that these brain regions could also be altered in B1-/- mice. We observed for the first time a difference in the gene expression pattern of CART (cocaine-and-amphetamine related transcript) in the LHA (lateral hypothalamic area) resulting from the deletion of the kinin B1 receptor gene. The correlation between CART expression in the LHA and the thwarting of diet-induced obesity corroborates independent correlations between CART and obesity. Further it seems to indicate that the mechanism underlying the 'lean' phenotype of B1-/- mice is not solely stemming from changes in peripheral tissues but may also receive contributions from changes in the hypothalamic machinery involved in energy homeostasis processes

Exploring skewed parton distributions with two body models on the light front II: covariant Bethe-Salpeter approach

We explore skewed parton distributions for two-body, light-front wave functions. In order to access all kinematical regimes, we adopt a covariant Bethe-Salpeter approach, which makes use of the underlying equation of motion (here the Weinberg equation) and its Green's function. Such an approach allows for the consistent treatment of the non-wave function vertex (but rules out the case of phenomenological wave functions derived from ad hoc potentials). Our investigation centers around checking internal consistency by demonstrating time-reversal invariance and continuity between valence and non-valence regimes. We derive our expressions by assuming the effective qq potential is independent of the mass squared, and verify the sum rule in a non-relativistic approximation in which the potential is energy independent. We consider bare-coupling as well as interacting skewed parton distributions and develop approximations for the Green's function which preserve the general properties of these distributions. Lastly we apply our approach to time-like form factors and find similar expressions for the related generalized distribution amplitudes.Comment: 25 pages, 12 figures, revised (minor changes but essential to consistency

A neutron scattering study of two-magnon states in the quantum magnet copper nitrate

We report measurements of the two-magnon states in a dimerized antiferromagnetic chain material, copper nitrate (Cu(NO3)2*2.5D2O). Using inelastic neutron scattering we have measured the one and two magnon excitation spectra in a large single crystal. The data are in excellent agreement with a perturbative expansion of the alternating Heisenberg Hamiltonian from the strongly dimerized limit. The expansion predicts a two-magnon bound state for q ~ (2n+1)pi*d which is consistent with the neutron scattering data.Comment: 11 pages of revtex style with 6 figures include

UPLC-MS-ESI-QTOF analysis and antifungal activity of the spondias tuberosa arruda leaf and root hydroalcoholic extracts

The aim of this study was to identify and evaluate the chemical compositions and effects of the S. tuberosa leaf and root hydroalcoholic extracts (HELST and HERST) against different strains of Candida. Chemical analysis was performed by Ultra-Performance Liquid Chromatography Coupled to Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF). The Inhibitory Concentration of 50% of the growth (IC50) as well as the intrinsic and combined action of the extracts with the antifungal fluconazole (FCZ) were determined by the microdilution method while the minimum fungicidal concentrations (MFCs) and the effect on fungal morphological transitions were analyzed by subculture and in humid chambers, respectively. From the preliminary phytochemical analysis, the phenols and flavonoids were the most abundant. The intrinsic IC50 values for HELST ranged from 5716.3 to 7805.8 \ub5g/mL and from 6175.4 to 51070.9 \ub5g/mL for the HERST, whereas the combination of the extracts with fluconazole presented IC50 values from 2.65 to 278.41 \ub5g/mL. The MFC of the extracts, individually, for all the tested strains was 6516384 \ub5g/mL. When fluconazole was combined with each extract, the MFC against CA URM 5974 was reduced (HELST: 2048 and HERST: 4096 \ub5g/mL). Synergism was observed against standard C. albicans (CA) and C. tropicalis (CT) strains and with the root extract against the CT isolate. The leaf extract inhibited the morphological transition of all strains while the root extract inhibited only CT strains

Search for CP violation in D0 and D+ decays

A high statistics sample of photoproduced charm particles from the FOCUS (E831) experiment at Fermilab has been used to search for CP violation in the Cabibbo suppressed decay modes D+ to K-K+pi+, D0 to K-K+ and D0 to pi-pi+. We have measured the following CP asymmetry parameters: A_CP(K-K+pi+) = +0.006 +/- 0.011 +/- 0.005, A_CP(K-K+) = -0.001 +/- 0.022 +/- 0.015 and A_CP(pi-pi+) = +0.048 +/- 0.039 +/- 0.025 where the first error is statistical and the second error is systematic. These asymmetries are consistent with zero with smaller errors than previous measurements.Comment: 12 pages, 4 figure

A measurement of lifetime differences in the neutral D-meson system

Using a high statistics sample of photoproduced charm particles from the FOCUS experiment at Fermilab, we compare the lifetimes of neutral D mesons decaying via D0 to K- pi+ and K- K+ to measure the lifetime differences between CP even and CP odd final states. These measurements bear on the phenomenology of D0 - D0bar mixing. If the D0 to K-pi+ is an equal mixture of CP even and CP odd eigenstates, we measure yCP = 0.0342 \pm 0.0139 \pm 0.0074.Comment: 15 pages, 5 figure