Effect of two different protein/fat ratios of the diet on meagre (<i>Argyrosomus regius</i>) traits

The aim of this study was to evaluate the effect of two diets with different protein/fat ratios (P/F) (diet A: P/F 2.26; diet B: P/F 3.36) on the chemical composition, fatty acid profile and some somatic indexes of meagre (Argyrosomus regius). The trial was carried out on two groups of meagre raised in two different sea cages during 15 months. At the end of the production cycle biometric measures as well as chemical-nutritional analysis of the fillets were conducted on 25 fishes per group. Diet A, with a lower P/F, furnished animals with higher percentages of mesenteric fat (0.48 vs 0.41%; P&lt;0.01) and of fillet yield (51.21 vs 48.12; P&lt;0.01). Moreover, the fillets obtained with the diet A showed higher percentage of fat (3.60 vs 2.41%; P&lt;0.01), lower moisture (74.10 vs 75.42%; P&lt;0.01), lower losses of water under pressure (16.73 vs 20.20%; P&lt;0.01) and after 48 h of refrigeration (3.08 vs 4.23%; P&lt;0.01). The fatty acids profile of fillets was affected by the diet. Diet A resulted in a higher level of saturated fatty acids (26.44 vs 23.17% of total lipid; P&lt;0.01) and a lower percentage of polyunsaturated fatty acids (31.56 vs 36.08%; P&lt;0.01) in the fillet, mainly due to the lower content of linoleic acid (13.63 vs 19.77%; P&lt;0.01). The atherogenic (AI) and thrombogenic (TI) indexes, which resulted very low in the fish of Group B (AI=0.48 vs 0.60, P&lt;0.01; TI=0.33 vs 0.37, P&lt;0.01), together with the low lipid content of meat in both groups, confirmed the very high nutritional quality of meagre fillets

Effect of two different protein/fat ratios of the diet on meagre (Argyrosomus regius) traits

The aim of this study was to evaluate the effect of two diets with different protein/fat ratios (P/F) (diet A: P/F 2.26; diet B: P/F 3.36) on the chemical composition, fatty acid profile and some somatic indexes of meagre (Argyrosomus regius). The trial was carried out on two groups of meagre raised in two different sea cages during 15 months. At the end of the production cycle biometric measures as well as chemical-nutritional analysis of the fillets were conducted on 25 fishes per group. Diet A, with a lower P/F, furnished animals with higher percentages of mesenteric fat (0.48 vs 0.41%; P<0.01) and of fillet yield (51.21 vs 48.12; P<0.01). Moreover, the fillets obtained with the diet A showed higher percentage of fat (3.60 vs 2.41%; P<0.01), lower moisture (74.10 vs 75.42%; P<0.01), lower losses of water under pressure (16.73 vs 20.20%; P<0.01) and after 48 h of refrigeration (3.08 vs 4.23%; P<0.01). The fatty acids profile of fillets was affected by the diet. Diet A resulted in a higher level of saturated fatty acids (26.44 vs 23.17% of total lipid; P<0.01) and a lower percentage of polyunsaturated fatty acids (31.56 vs 36.08%; P<0.01) in the fillet, mainly due to the lower content of linoleic acid (13.63 vs 19.77%; P<0.01). The atherogenic (AI) and thrombogenic (TI) indexes, which resulted very low in the fish of Group B (AI=0.48 vs 0.60, P<0.01; TI=0.33 vs 0.37, P<0.01), together with the low lipid content of meat in both groups, confirmed the very high nutritional quality of meagre fillets

Anti-Matter in Cosmic Rays : Backgrounds and Signals

Recent PAMELA and ATIC data seem to indicate an excess in positron cosmic rays above approximately 10 GeV which might be due to galactic Dark Matter particle annihilation. However the background of this signal suffers many uncertainties that make our task difficult in constraining Dark Matter or any other astrophysical explanation for these recent surprising data.Comment: Proceedings for XLIVemes rencontres de Moriond, Electroweak Interactions And Unified Theories sessio

Influence of <i>Moraxella</i> sp. colonization on the kidney proteome of farmed gilthead sea breams (<i>Sparus aurata</i>, L.)

Background: Currently, presence of Moraxella sp. in internal organs of fish is not considered detrimental for fish farming. However, bacterial colonization of internal organs can affect fish wellness and decrease growth rate, stress resistance, and immune response. Recently, there have been reports by farmers concerning slow growth, poor feed conversion, and low average weight increase of fish farmed in offshore floating sea cages, often associated with internal organ colonization by Moraxella sp. Therefore, presence of these opportunistic bacteria deserves further investigations for elucidating incidence and impact on fish metabolism. Results: A total of 960 gilthead sea breams (Sparus aurata, L.), collected along 17 months from four offshore sea cage plants and two natural lagoons in Sardinia, were subjected to routine microbiological examination of internal organs throughout the production cycle. Thirteen subjects (1.35%) were found positive for Moraxella sp. in the kidney (7), brain (3), eye (1), spleen (1), and perivisceral fat (1). In order to investigate the influence of Moraxella sp. colonization, positive and negative kidney samples were subjected to a differential proteomics study by means of 2-D PAGE and mass spectrometry. Interestingly, Moraxella sp. infected kidneys displayed a concerted upregulation of several mitochondrial enzymes compared to negative tissues, reinforcing previous observations following lipopolysaccharide (LPS) challenge in fish. Conclusions: Presence of Moraxella sp. in farmed sea bream kidney is able to induce proteome alterations similar to those described following LPS challenge in other fish species. This study revealed that Moraxella sp. might becausing metabolic alterations in fish, and provided indications on proteins that could be investigated as markers of infection by Gram-negative bacteria within farming plants

Influence of Moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (Sparus aurata, L.)

Background: Currently, presence of Moraxella sp. in internal organs of fish is not considered detrimental for fish farming. However, bacterial colonization of internal organs can affect fish wellness and decrease growth rate, stress resistance, and immune response. Recently, there have been reports by farmers concerning slow growth, poor feed conversion, and low average weight increase of fish farmed in offshore floating sea cages, often associated with internal organ colonization by Moraxella sp. Therefore, presence of these opportunistic bacteria deserves further investigations for elucidating incidence and impact on fish metabolism. Results: A total of 960 gilthead sea breams (Sparus aurata, L.), collected along 17 months from four offshore sea cage plants and two natural lagoons in Sardinia, were subjected to routine microbiological examination of internal organs throughout the production cycle. Thirteen subjects (1.35%) were found positive for Moraxella sp. in the kidney (7), brain (3), eye (1), spleen (1), and perivisceral fat (1). In order to investigate the influence of Moraxella sp. colonization, positive and negative kidney samples were subjected to a differential proteomics study by means of 2-D PAGE and mass spectrometry. Interestingly, Moraxella sp. infected kidneys displayed a concerted upregulation of several mitochondrial enzymes compared to negative tissues, reinforcing previous observations following lipopolysaccharide (LPS) challenge in fish. Conclusions: Presence of Moraxella sp. in farmed sea bream kidney is able to induce proteome alterations similar to those described following LPS challenge in other fish species. This study revealed that Moraxella sp. might be causing metabolic alterations in fish, and provided indications on proteins that could be investigated as markers of infection by Gram-negative bacteria within farming plants.Pubblicat

The Impact of Locoregional Treatment on Response to Nivolumab in Advanced Platinum Refractory Head and Neck Cancer: The Need Trial

Background: Previous locoregional treatment could affect the response to nivolumab in platinum-refractory recurrent/metastatic head and neck squamous cell carcinoma (R/M HNSCC). The aim of this study is to evaluate the impact of the clinicopathological characteristics and previous treatment in predicting early progression to nivolumab in a real-world population. Methods: This is an observational, multicenter retrospective/prospective study including patients (pts) with platinum refractory R/M HNSCC who received nivolumab 240 mg every 2 weeks from October 2018 to October 2019. We analyzed the association between previous treatment, clinicopathological characteristics, and early progression (within 3 months). Results: Data from 61 pts were reviewed. Median age was 67 years (30–82). Forty-two pts (69%) received previous locoregional treatment. Early progression to nivolumab occurred in 36 pts (59%), while clinical benefit (stable disease and partial response) was achieved in 25 pts (41%). Early progression to nivolumab was significantly associated to previous locoregional treatment both at univariate and multivariate analysis (p = 0.005 and p = 0.048, respectively). Conclusion: nivolumab in R/M HNSCC is burdened with a high early progression rate. Previous wide neck dissection and high dose radiotherapy may compromise the efficacy of nivolumab, distorting the anatomy of the local lymphatic system and hindering the priming of immune response

Etude de positons d'interactions des rayons cosmiques at annihilations de matière sombre dans l'environnement galactique

Rayons cosmiques de positons et les électrons de fournir un moyen complémentaire à l'étude de l'environnement galactique. Les expériences de rayons cosmiques, par exemple PAMELA et HEAT, ont présenté des résultats très intéressant dans ce domaine. La fraction de positrons observé semble plus importante que la réalité les prédictions théoriques des énergies de plus de 10 GeV. Les preuves indirectes de la matière sombre dans le cadre de théories au-delà du modèle standard suggère l'existence d'une contribution supplémentaire présent dans le signal de rayons cosmiques. Nous étudions et le calcul de la positon du signal produit par l'anéantissement d'un générique de Dark Matter candidate. En particulier, nous analysons les signatures typiques annihilation et de l'impact de la physique sur la propagation CR positon signal. En outre, nous étudions la positon signal liées à des processus de spallation entre noyaux cosmiques - les rayons et le gaz interstellaire. Nous analysons les effets de l'incertitude actuelle en matière de coupe, les noyaux cosmiques - ray et CR propagation physique. La propagation de positons est modelé en fonction de la Deux - Zone modèle de propagation qui a été testé avec succès dans l'étude des noyaux cosmiques - ray et présente une approche analytique pour étudier les cosmique - physique des rayons.Positron and electron cosmic rays provide a complementary way to study the galactic environment. The actual cosmic rays experiments, for instance PAMELA and HEAT, have presented very exciting results in this field. The observed positron fraction appears larger than the actual theoretical predictions for energies larger than 10 GeV. The indirect evidences of Dark Matter in connection with Beyond the Standard Model theories would suggest the existence of an extra contribution present in the cosmic ray signal. We study and calculate the positron signal produced by the annihilation of a generic Dark Matter candidate. Especially, We analyze typical annihilation signatures and the impact of CR propagation physics on the positron signal. In addition, we study the positron signal related to spallation processes between nuclei cosmic-rays and the interstellar gas. We analyze the effects of uncertainties present in nuclear cross section, nuclei cosmic-ray and CR propagation physics. The propagation of positrons is modeled according to the Two-Zone Propagation Model which has been successfully tested in the study of nuclei cosmic-ray and present an analytical approach to study the cosmic ray physics.CHAMBERY -BU Bourget (730512101) / SudocSudocFranceF

FAILURE TO DETECT GENOMIC MATERIAL OF HTLV-I OR HTLV-II IN MONONUCLEAR-CELLS OF ITALIAN PATIENTS WITH MULTIPLE-SCLEROSIS AND CHRONIC PROGRESSIVE MYELOPATHY

To contribute to the undecided question if a retrovirus of the human T-cell lymphotropic virus (HTLV) family may be involved in the development of multiple sclerosis (MS), we investigated by the polymerase chain reaction (PCR) the presence of HTLV-I and HTLV-II sequences in the peripheral blood mononuclear cell DNAs from 30 patients affected by MS and 15 by chronic progressive myelopathy. Moreover a control group of 14 blood donors was examined. All these patients were devoid of anti-HTLV-I antibody in the serum and cerebrospinal fluid at ELISA. For the PCR, primers and probes, specific for the tax region common to HTLV-I and HTLV-II, for the pol region of HTLV-I, and for the pol region of HTLV-II were used. In spite of the high sensitivity of the technique used, the three groups of subjects were negative for HTLV-I and HTLV-II genomic sequences