On the primordial scenario for abundance variations within globular clusters. The isochrone test

Self-enrichment processes occurring in the early stages of a globular cluster lifetime are generally invoked to explain the observed CNONaMgAl abundance anticorrelations within individual Galactic globulars.We have tested, with fully consistent stellar evolution calculations, if theoretical isochrones for stars born with the observed abundance anticorrelations satisfy the observational evidence that objects with different degrees of these anomalies lie on essentially identical sequences in the Color-Magnitude-Diagram (CMD). To this purpose, we have computed for the first time low-mass stellar models and isochrones with an initial metal mixture that includes the extreme values of the observed abundance anticorrelations, and varying initial He mass fractions. Comparisons with 'normal' alpha-enhanced isochrones and suitable Monte Carlo simulations that include photometric errors show that a significant broadening of the CMD sequences occurs only if the helium enhancement is extremely large (in this study, when Y=0.35) in the stars showing anomalous abundances. Stellar luminosity functions up to the Red Giant Branch tip are also very weakly affected, apart from - depending on the He content of the polluting material - the Red Giant Branch bump region. We also study the distribution of stars along the Zero Age Horizontal Branch, and derive general constraints on the relative location of objects with and without abundance anomalies along the observed horizontal branches of globular clusters.Comment: Accepted for publication in Ap

The Role of Dust in Models of Population Synthesis

We have employed state-of-the-art evolutionary models of low and intermediate-mass AGB stars, and included the effect of circumstellar dust shells on the spectral energy distribution (SED) of AGB stars, to revise the Padua library of isochrones (Bertelli et al. 1994). The major revision involves the thermally pulsing AGB phase, that is now taken from fully evolutionary calculations by Weiss & Ferguson (2009). Two libraries of about 600 AGB dust-enshrouded SEDs each have also been calculated, one for oxygen-rich M-stars and one for carbon-rich C-stars. Each library accounts for different values of input parameters like the optical depth {\tau}, dust composition, and temperature of the inner boundary of the dust shell. These libraries of dusty AGB spectra have been implemented into a large composite library of theoretical stellar spectra, to cover all regions of the Hertzsprung-Russell Diagram (HRD) crossed by the isochrones. With the aid of the above isochrones and libraries of stellar SEDs, we have calculated the spectro-photometric properties (SEDs, magnitudes, and colours) of single-generation stellar populations (SSPs) for six metallicities, more than fifty ages (from 3 Myr to 15 Gyr), and nine choices of the Initial Mass Function. The new isochrones and SSPs have been compared to the colour-magnitude diagrams (CMDs) of field populations in the LMC and SMC, with particular emphasis on AGB stars, and the integrated colours of star clusters in the same galaxies, using data from the SAGE (Surveying the Agents of Galaxy Evolution) catalogues. We have also examined the integrated colours of a small sample of star clusters located in the outskirts of M31. The agreement between theory and observations is generally good. In particular, the new SSPs reproduce the red tails of the AGB star distribution in the CMDs of field stars in the Magellanic Clouds.Comment: Accepted for publication in MNRA

A large stellar evolution database for population synthesis studies. II. Stellar models and isochrones for an alpha-enhanced metal distribution

[Abridged] We present a large, new set of stellar evolution models and isochrones for an alpha-enhanced metal distribution typical of Galactic halo and bulge stars; it represents a homogeneous extension of our stellar model library for a distribution already presented in Pietrinferni et al.(2004). The effect of the alpha-element enhancement has been properly taken into account in the nuclear network, opacity, equation of state and, for the first time, the bolometric corrections, and color transformations. This allows us to avoid the inconsistent use - common to all alpha-enhanced model libraries currently available - of scaled-solar bolometric corrections and color transformations for alpha-enhanced models and isochrones. We show how bolometric corrections to magnitudes obtained for the U,B portion of stellar spectra for T_{eff}<=6500K, are significantly affected by the metal mixture, especially at the higher metallicities. We also provide complete sets of evolutionary models for low-mass, He-burning stellar structures covering the whole metallicity range, to enable synthetic horizontal branch simulations. We compare our database with several widely used stellar model libraries from different authors, as well as with various observed color magnitude and color-color diagrams (Johnson-Cousins BVI and near infrared magnitudes, Stromgren colors) of Galactic field stars and globular clusters. We also test our isochrones comparing integrated optical colors and Surface Brightness Fluctuation magnitudes with selected globular cluster data. We find a general satisfactory agreement with the empirical constraints.Comment: 46 pages, 20 figures, ApJ in press, the whole database presented in this paper can be foud at http://www.te.astro.it/BASTI/index.ph

A Rapid, Widely Applicable Screen for Drugs that Suppress Free Radical Formation in Ischemia/Reperfusion

Substantial injury can occur during reoxygenation of previously ischemic tissue in many experimental models, as the result of the generation of oxygen-derived free radicals. To test the antiradical activity of potentially protective compounds in this setting, we developed a simple screening system, applicable to fresh biopsy specimens, in which warm ischemia and reoxygenation of excised tissue are performed in vitro. Tissue production of malondialdehyde (MDA) equivalents is used as a nonspecific-but-sensitive marker of oxygen radical damage. Test compounds with putative antiradical activity are added prior to the reoxygenation phase, and their ability to suppress MDA production is an index of activity in preventing reoxygenation injury. Comparison with ischemic but not reoxygenated controls confirms the oxygen-dependent nature of the effect. Standard positive controls of known effective agents, such as butylated hydroxytoluene or deferoxamine, provide a reference for the activity of the test compound. The method is applicable to surgical biopsy specimens in veterinary and human medicine

Optical Identification of He White Dwarfs Orbiting Four Millisecond Pulsars in the Globular Cluster 47 Tucanae

We used ultra-deep UV observations obtained with the Hubble Space Telescope to search for optical companions to binary millisecond pulsars (MSPs) in the globular cluster 47 Tucanae. We identified four new counterparts (to MSPs 47TucQ, 47TucS, 47TucT and 47TucY) and confirmed those already known (to MSPs 47TucU and 47TucW). In the color magnitude diagram, the detected companions are located in a region between the main sequence and the CO white dwarf cooling sequences, consistent with the cooling tracks of He white dwarfs of mass between 0.15 Msun and 0.20 Msun. For each identified companion, mass, cooling age, temperature and pulsar mass (as a function of the inclination angle) have been derived and discussed. For 47TucU we also found that the past accretion history likely proceeded in a sub-Eddington rate. The companion to the redback 47TucW is confirmed to be a non degenerate star, with properties particularly similar to those observed for black widow systems. Two stars have been identified within the 2-sigma astrometric uncertainty from the radio positions of 47TucH and 47TucI, but the available data prevent us from firmly assessing whether they are the true companions of these two MSPs.Comment: 27 pages, 7 figures, Accepted for publication by Ap

The optical companion to the intermediate mass millisecond pulsar J1439-5501 in the Galactic field

We present the identification of the companion star to the intermediate mass binary pulsar J1439-5501 obtained by means of ground-based deep images in the B, V and I bands, acquired with FORS2 mounted at the ESO-VLT. The companion is a massive white dwarf (WD) with B=23.57+-0.02, V=23.21+-0.01 and I=22.96+-0.01, located at only ~0.05" from the pulsar radio position. Comparing the WD location in the (B, B-V) and (V, V-I) Color-Magnitude diagrams with theoretical cooling sequences we derived a range of plausible combinations of companion masses (1<~Mcom<~1.3 Msun), distances (d<~1200 pc), radii (<~7.8 10^3 Rsun) and temperatures (T=31350^{+21500}_{-7400}). From the PSR mass function and the estimated mass range we also constrained the inclination angle i >~ 55 degrees and the pulsar mass (Mpsr <~2.2 Msun). The comparison between the WD cooling age and the spin down age suggests that the latter is overestimated by a factor of about ten.Comment: Accepted for publication by ApJ; 19 pages, 5 figures, 1 tabl

A Bound on the Light Emitted During the TP-AGB Phase

The integrated luminosity of the TP-AGB phase is a major uncertainty in stellar population synthesis models. We use the white dwarf initial final mass relation and stellar interiors models to demonstrate that a significant fraction of the core mass growth for intermediate (1.5 < Msun < 6) mass stars takes place during the TP-AGB phase. We find evidence that the peak fractional core mass contribution for TP-AGB stars is ~20% and occurs for stars between 2 Msun and 3.5 Msun. Using a simple fuel consumption argument we couple this core mass increase to a lower limit on the TP-AGB phase energy output. Roughly half of the energy released in models of TP-AGB stars can be directly accounted for by this core growth; while the remainder is predominantly the stellar yield of He. A robust measurement of the emitted light in this phase will therefore set strong constraints on helium enrichment from TP-AGB stars, and we estimate the yields predicted by current models as a function of initial mass. Implications for stellar population studies and prospects for improvements are discussed.Comment: Submitted to the Astrophysical Journal. 25 pages, 2 figures

Methylene Blue as an Inhibitor of Superoxide Generation by Xanthene Oxidase: A Potential New Drug for the Attenuation of Ischemia/Reperfusion Injury

Tissue oxidases, especially xanthine oxidase, have been proposed as primary sources of toxic oxygen radicals in many experimental models of disease states. Among these, ischemia-reperfusion injury may be of the greatest clinical interest. In this paper we propose the use of methylene blue as a means of suppressing the production of superoxide radicals, O2, by acting as an alternative electron acceptor for xanthine oxidase. Previous work has indicated that methylene blue accepts electrons from xanthine oxidase at the iron-sulfur center. Initial experiments in our laboratory demonstrated that (1) pairs of electrons from each enzymatic oxidation are transferred to methylene blue, (2) the reduction of methylene blue can be achieved by model iron-sulfur centers, similar to the iron-sulfur center of xanthine oxidase, (3) reduced methylene blue auto-oxidizes to produce H2O2 directly, rather than O2, and (4) methylene blue is effective at non-toxic levels (2-5 mg/kg) in preventing free radical damage to liver and kidney tissues in an in vitro model of ischemia and reoxygenation. Accordingly, we propose that methylene blue may represent a new class of antioxidant drugs that competitively inhibit reduction of molecular oxygen to superoxide by acting as alternative electron acceptors for tissue oxidases. We have termed these agents “parasitic” electron acceptors

Evidence That Free Fatty Acid-Iron Complexes Directly Initiate Lipid Peroxidation in Vitro and in Vivo: A New Mechanism of Oxidative Stress

Through a series of biochemical and histochemical experiments we explored the novel hypothesis that iron and free fatty acids, liberated after tissue injury, combine to form liposoluble complexes that directly initiate lipid peroxidation. The addition of 100 M ferric iron to 30 mM linoleate suspensions at pH 7.4 produced time dependent lipid peroxidation, measured as conjugated diene formation. Complexes of 100 M ferric iron and 600 M pentanoate also initiated formation of conjugated dienes in linoleate suspensions and formation of malondialdehyde-like materials in rat liver slices. A histochemical stain for free fatty acids revealed positive reactions within cell membranes in traumatized regions of rat liver tissue that underwent compression injury followed by thirty minutes of blood perfusion, but not in nontraumatized control regions. The diaminobenzidine-H2O2 histochemical reaction for iron, revealed increased levels of redox cyclable iron in the membranes and the cytoplasm of traumatized hepatocytes. We propose that traumatic injury initiates cascades leading to liberation of iron from storage proteins and free fatty acids from membranes, which combine, distribute to the lipid domains of cell membranes, and directly initiate lipid peroxidation