Effects of cadmium perturbation on the microbial community structure and heavy metal resistome of a tropical agricultural soil

The effects of cadmium (Cd) contamination on the microbial community structure, soil physicochemistry and heavy metal resistome of a tropical agricultural soil were evaluated in field-moist soil microcosms. A Cd-contaminated agricultural soil (SL5) and an untreated control (SL4) were compared over a period of 5 weeks. Analysis of the physicochemical properties and heavy metals content of the two microcosms revealed a statistically significant decrease in value of the soil physicochemical parameters (P < 0.05) and concentration of heavy metals (Cd, Pb, Cr, Zn, Fe, Cu, Se) content of the agricultural soil in SL5 microcosm. Illumina shotgun sequencing of the DNA extracted from the two microcosms showed the predominance of the phyla, classes, genera and species of Proteobacteria (37.38%), Actinobacteria (35.02%), Prevotella (6.93%), and Conexibacter woesei (8.93%) in SL4, and Proteobacteria (50.50%), Alphaproteobacteria (22.28%), Methylobacterium (9.14%), and Methylobacterium radiotolerans (12,80%) in SL5, respectively. Statistically significant (P < 0.05) difference between the metagenomes was observed at genus and species delineations. Functional annotation of the two metagenomes revealed diverse heavy metal resistome for the uptake, transport, efflux and detoxification of various heavy metals. It also revealed the exclusive detection in SL5 metagenome of members of RND (resistance nodulation division) protein czcCBA efflux system (czcA, czrA, czrB), CDF (cation diffusion facilitator) transporters (czcD), and genes for enzymes that protect the microbial cells against cadmium stress (sodA, sodB, ahpC). The results obtained in this study showed that Cd contamination significantly affects the soil microbial community structure and function, modifies the heavy metal resistome, alters the soil physicochemistry and results in massive loss of some autochthonous members of the community not adapted to the Cd stress

Modelling Flood Hazards Impacted by Ungauged River in Urbanised Area Using HEC-RAS and GIS

Ilorin City is located downstream of Asa, Oyun and Awun River Basins. The Asa River is a major river that traverses the city and divides the metropolitan area into east and west, almost equally. The river often overflows its banks to inundate adjacent communities, influencing severe economic damage and impact on human lives. However, efforts to mitigate this have majorly been focused on dredging the Asa River channel which has not solved the problem. For an accurate spatial and temporal understanding of the risks of floods and their potential hazards, it is important to estimate floods using river hydrology. The objective of this study is to model steady flow of the rivers using flow data and to map flood-prone areas in Ilorin using HEC-RAS integrated with GIS. Using the HEC-GeoRAS extension in the GIS environment, the geometric data of the rivers were obtained from the 30 m resolution digital elevation model (DEM) and input into HEC-RAS applying Manning Co-efficient values of 0.04, 0.045, and 0.04. For each river, flow data (Q) was given as the upstream boundary condition while a normal depth of 0.001 was assigned for the downstream condition to model a steady flow and inundation extents. The result of the HEC-RAS model has shown the flood-prone areas along the river channels delineated. The floodplain map produced reveals the spatial distribution and extent of the high flood-risk areas in the Ilorin metropolis. The total flooded area covers approximately 60.95 km2 (18%) majorly along the river channels. This study has demonstrated that integration of hydraulic modelling using HEC-RAS and GIS process is capable of producing an inundation flood map with good accuracy that will aid in suggesting effective measures to mitigate the impact of flooding

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries