The truncation of the disk of NGC 4565: Detected up to z=4 kpc, with star formation, and affected by the warp

Context: The hierarchical model of galaxy formation suggests that galaxies are continuously growing. However, our position inside the Milky Way prevents us from studying the disk edge. Truncations are low surface brightness features located in the disk outskirts of external galaxies. They indicate where the disk brightness abruptly drops and their location is thought to change dynamically. In previous analyses of Milky Way-like galaxies, truncations were detected up to 3 kpc above the mid-plane but whether they remain present beyond that height remains unclear. Aims: Our goal is to determine whether truncations can be detected above 3 kpc height in the Milky Way-like galaxy NGC 4565, thus establishing the actual disk thickness. We also aim to study how the truncation relates to disk properties such as star formation activity or the warp. Methods: We perform a vertical study of the disk of NGC 4565 edge in unprecedented detail. We explore the truncation radius at different heights above/below the disk mid-plane (0<z<8 kpc) and at different wavelengths. We use new ultra-deep optical data ($\mu_{g,\rm{lim}}=30.5$ mag arcsec$^{-2}$; $3 \sigma$ within $10 \times 10$ arcsec$^{2}$ boxes) in the $g$, $r$ and $i$ broad bands, along with near- and far-ultraviolet, H$\alpha$, and \ion{H}{i} observations. Results: We detect the truncation up to 4 kpc in the $g$, $r$ and $i$ ultra-deep bands which is 1 kpc higher than in any previous study for any galaxy. The radial position of the truncation remains constant up to 3 kpc while higher up it is located at a smaller radius. This result is independent of the wavelength but is affected by the presence of the warp. Conclusions: We propose an inside-out growth scenario for the formation of the disk of NGC 4565. Our results point towards the truncation feature being linked to a star-forming threshold and to the onset of the disk warp.Comment: 27 pages, 18 figures (incl. 2 appendix); accepted for publication in A&A; Fixed labels in Fig.

Divulgación científica: aprender haciendo y coevaluando

[EN] Objective/s: To assess the process of acquiring competence in scientific dissemination among students at the La Fe School of Nursing in Valencia. Development of the innovation: Four cycles of group work were implemented. Each group creates scientific dissemination documents focused on a work topic related to the subject of Physical Activity and Health Promotion in Nursing. Each work is co-evaluated by peers using an analytical rubric, with 10 learning domains, and this evaluation is returned before the creation of the next document. At the end of the 4 work cycles, 8 teachers external to the activity assessed the dissemination papers using the same co-assessment rubric, anonymously and blinded. Results: Acquisition of competence in the domains studied showed significant improvements with repetition of the tasks. The study differentiated by groups shows that learning becomes uniform in 6 of the 10 competencies, showing significant differences in learning between groups in 4 of the domains. Conclusions: Repetition of the task and co-assessment improves the acquisition of competences in science popularisation. The moment of greatest evolution occurs between the second and third repetition of the task, slowing down this benefit between the third and fourth repetition.[ES] Objetivo: Valorar el proceso de adquisición de la competencia en divulgación científica del alumnado de la Escuela de Enfermería de la Fe de Valencia. Desarrollo de la innovación: Se implementaron cuatro ciclos de trabajo grupal. Cada grupo crea documentos de divulgación científica centrados en una temática de trabajo relacionada con la asignatura de Actividad física y Promoción de la salud en Enfermería. Cada trabajo es coevaluado por pares mediante rúbrica analítica, con 10 dominios de aprendizaje, y se devuelve esta evaluación antes de la creación del siguiente documento. Finalizados los 4 ciclos de trabajo, 8 docentes externos a la actividad valoraron los documentos de divulgación utilizando la misma rúbrica de coevaluación, de forma anónima y cegada. Resultados: La adquisición de competencia en los dominios estudiados mostraron mejoras significativas con la repetición de las tareas. En el estudio diferenciado por grupos se muestra que el aprendizaje se vuelve uniformen en 6 de las 10 competencias, mostrando diferencias significativas de aprendizaje entre grupos en 4 de los dominios. Conclusiones: La repetición de la tarea y la coevaluación mejora la adquisición de competencias en divulgación científica. El momento de mayor evolución se produce entre la segunda y tercera repetición de la tarea, ralentizando este beneficio entre la tercera y cuarta repetición.García-Martinez, P.; Saus-Ortega, C.; García-Molina, P.; Balaguer-López, E.; Celda-Belinchón, L.; Sosa-Palanca, E.; Buck Sainz-Rozas, P.... (2022). Divulgación científica: aprender haciendo y coevaluando. Editorial Universitat Politècnica de València. 161-172. https://doi.org/10.4995/INRED2022.2022.1583516117

Obstetric outcomes of sars-cov-2 infection in asymptomatic pregnant women

Altres ajuts: Fondo Europeo de Desarrollo Regional (FEDER)Around two percent of asymptomatic women in labor test positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Spain. Families and care providers face childbirth with uncertainty. We determined if SARS-CoV-2 infection at delivery among asymptomatic mothers had different obstetric outcomes compared to negative patients. This was a multicenter prospective study based on universal antenatal screening for SARS-CoV-2 infection. A total of 42 hospitals tested women admitted for delivery using polymerase chain reaction, from March to May 2020. We included positive mothers and a sample of negative mothers asymptomatic throughout the antenatal period, with 6-week postpartum follow-up. Association between SARS-CoV-2 and obstetric outcomes was evaluated by multivariate logistic regression analyses. In total, 174 asymptomatic SARS-CoV-2 positive pregnancies were compared with 430 asymptomatic negative pregnancies. No differences were observed between both groups in key maternal and neonatal outcomes at delivery and follow-up, with the exception of prelabor rupture of membranes at term (adjusted odds ratio 1.88, 95% confidence interval 1.13-3.11; p = 0.015). Asymptomatic SARS-CoV-2 positive mothers have higher odds of prelabor rupture of membranes at term, without an increase in perinatal complications, compared to negative mothers. Pregnant women testing positive for SARS-CoV-2 at admission for delivery should be reassured by their healthcare workers in the absence of symptoms

Natural History of MYH7-Related Dilated Cardiomyopathy

BACKGROUND Variants in myosin heavy chain 7 (MYH7) are responsible for disease in 1% to 5% of patients with dilated cardiomyopathy (DCM); however, the clinical characteristics and natural history of MYH7-related DCM are poorly described. OBJECTIVES We sought to determine the phenotype and prognosis of MYH7-related DCM. We also evaluated the influence of variant location on phenotypic expression. METHODS We studied clinical data from 147 individuals with DCM-causing MYH7 variants (47.6% female; 35.6 +/- 19.2 years) recruited from 29 international centers. RESULTS At initial evaluation, 106 (72.1%) patients had DCM (left ventricular ejection fraction: 34.5% +/- 11.7%). Median follow-up was 4.5 years (IQR: 1.7-8.0 years), and 23.7% of carriers who were initially phenotype-negative developed DCM. Phenotypic expression by 40 and 60 years was 46% and 88%, respectively, with 18 patients (16%) first diagnosed at <18 years of age. Thirty-six percent of patients with DCM met imaging criteria for LV noncompaction. During follow-up, 28% showed left ventricular reverse remodeling. Incidence of adverse cardiac events among patients with DCM at 5 years was 11.6%, with 5 (4.6%) deaths caused by end-stage heart failure (ESHF) and 5 patients (4.6%) requiring heart transplantation. The major ventricular arrhythmia rate was low (1.0% and 2.1% at 5 years in patients with DCM and in those with LVEF of <= 35%, respectively). ESHF and major ventricular arrhythmia were significantly lower compared with LMNA-related DCM and similar to DCM caused by TTN truncating variants. CONCLUSIONS MYH7-related DCM is characterized by early age of onset, high phenotypic expression, low left ventricular reverse remodeling, and frequent progression to ESHF. Heart failure complications predominate over ventricular arrhythmias, which are rare. (C) 2022 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Spatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions

Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41–6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain.Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000–2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method in BEAST v1.8.0 and by reconstructing ancestral states using the criterion of parsimony in Mesquite v3.4, respectively.Results: The HIV-1 CRF02_AG prevalence differed across Spanish autonomous communities we sampled from (p &lt; 0.001). Phylogenetic analysis revealed that 52.7% of the CRF02_AG sequences formed 56 monophyletic clusters, with a range of 2–79 sequences. The CRF02_AG regional dispersal differed across Spain (p = 0.003), as suggested by monophyletic clustering. For the largest monophyletic cluster (subepidemic) (N = 79), 49.4% of the clustered sequences originated from Madrid, while most sequences (51.9%) had been obtained from men having sex with men (MSM). Molecular clock analysis suggested that the origin (tMRCA) of the CRF02_AG subepidemic was in 2002 (median estimate; 95% Highest Posterior Density-HPD interval: 1999–2004). Additionally, we found significant clustering within the CRF02_AG subepidemic according to the ethnic origin.Conclusion: CRF02_AG has been introduced as a result of multiple introductions in Spain, following regional dispersal in several cases. We showed that CRF02_AG transmissions were mostly due to regional dispersal in Spain. The hot-spot for the largest CRF02_AG regional subepidemic in Spain was in Madrid associated with MSM transmission risk group. The existence of subepidemics suggest that several spillovers occurred from Madrid to other areas. CRF02_AG sequences from Hispanics were clustered in a separate subclade suggesting no linkage between the local and Hispanic subepidemics

Time to Switch to Second-line Antiretroviral Therapy in Children With Human Immunodeficiency Virus in Europe and Thailand.

Background: Data on durability of first-line antiretroviral therapy (ART) in children with human immunodeficiency virus (HIV) are limited. We assessed time to switch to second-line therapy in 16 European countries and Thailand. Methods: Children aged <18 years initiating combination ART (≥2 nucleoside reverse transcriptase inhibitors [NRTIs] plus nonnucleoside reverse transcriptase inhibitor [NNRTI] or boosted protease inhibitor [PI]) were included. Switch to second-line was defined as (i) change across drug class (PI to NNRTI or vice versa) or within PI class plus change of ≥1 NRTI; (ii) change from single to dual PI; or (iii) addition of a new drug class. Cumulative incidence of switch was calculated with death and loss to follow-up as competing risks. Results: Of 3668 children included, median age at ART initiation was 6.1 (interquartile range (IQR), 1.7-10.5) years. Initial regimens were 32% PI based, 34% nevirapine (NVP) based, and 33% efavirenz based. Median duration of follow-up was 5.4 (IQR, 2.9-8.3) years. Cumulative incidence of switch at 5 years was 21% (95% confidence interval, 20%-23%), with significant regional variations. Median time to switch was 30 (IQR, 16-58) months; two-thirds of switches were related to treatment failure. In multivariable analysis, older age, severe immunosuppression and higher viral load (VL) at ART start, and NVP-based initial regimens were associated with increased risk of switch. Conclusions: One in 5 children switched to a second-line regimen by 5 years of ART, with two-thirds failure related. Advanced HIV, older age, and NVP-based regimens were associated with increased risk of switch

BigBrain-MR: a new digital phantom with anatomically-realistic magnetic resonance properties at 100-µm resolution for magnetic resonance methods development

The benefits, opportunities and growing availability of ultra-high field magnetic resonance imaging (MRI) for humans have prompted an expansion in research and development efforts towards increasingly more advanced high-resolution imaging techniques. To maximize their effectiveness, these efforts need to be supported by powerful computational simulation platforms that can adequately reproduce the biophysical characteristics of MRI, with high spatial resolution. In this work, we have sought to address this need by developing a novel digital phantom with realistic anatomical detail up to 100-µm resolution, including multiple MRI properties that affect image generation. This phantom, termed BigBrain-MR, was generated from the publicly available BigBrain histological dataset and lower-resolution in-vivo 7T-MRI data, using a newly-developed image processing framework that allows mapping the general properties of the latter into the fine anatomical scale of the former. Overall, the mapping framework was found to be effective and robust, yielding a diverse range of realistic “in-vivo-like” MRI contrasts and maps at 100-µm resolution. BigBrain-MR was then tested in three imaging applications (motion effects and interpolation, super-resolution imaging, and parallel imaging reconstruction) to investigate its properties, value and validity as a simulation platform. The results consistently showed that BigBrain-MR can closely approximate the behavior of real in-vivo data, more realistically and with more extensive features than a more classic option such as the Shepp-Logan phantom. Its flexibility in simulating different contrast mechanisms and artifacts may also prove valuable for educational applications. BigBrain-MR is therefore deemed a favorable choice to support methodological development and demonstration in brain MRI, and has been made freely available to the community