Identification and determination of cellulase activity of cellulose degrading microorganisms from earthworm species of different habitats of North East India

192-205A total of 327 microbial strains isolated from the earthworm guts (EWGs) of five different species were comprised of 93 aerobic bacteria (AB), 78 facultative anaerobic bacteria (FAB), 72 actinobacteria and 84 fungi. Cellulolytic activity was determined both by qualitative and quantitative methods. Sixteen AB, 19 FAB, 26 actinobacteria and 23 fungi showed hydrolyzing zones on carboxymethyl cellulose medium and varied cellulase activity in the range of 0.0031 to 0.0263, 0.0022 to 0.013, 0.0022 to 0.0154 and 0.005 to 0.036 IU/ml, respectively. Cellulolytic bacterial and fungal isolates were identified based on 16S rDNA and ITS1-5.8S-ITS2 region, respectively. Nine cellulolytic bacterial (Bacillus, Paenibacillus, Isoptericola, Cellulomonas, Clostridium, Enterobacter, Nocardia, Micromonospora and Streptomyces) and ten fungal genera (Aspergillus, Chaetomium, Acremonium, Fusarium, Penicillium, Purpureocillium, Epicoccum, Trichoderma, Talaromyces and Ascobolus) were recorded in the EWGs. Cellulolytic microorganisms of EWGs exhibit taxonomic diversity and varying cellulase activity depending upon EW habitats. This is the first report on the cellulolytic bacterial and fungal diversity from the EWG of Imphal, Manipur, North East, India

Two Dimensional Gel Electrophoresis (2DE) of extracellular protein of Talaromyces verruculosus SGMNPf3

The scientific data summarizes identified peptide spots from two dimensional gel electrophoresis. Identified spots are secretory products from the cellulolytic activity of Talaromyces verruculosus SGMNPf3. Peptide spots were identified by LC MS/MS and MALDI TOF-MS and detail characteristics of spots are provided

Data from: Unveiling the optimal parameters for cellulolytic characteristics of Talaromyces verruculosus SGMNPf3 and its secretory enzymes

AIMS: Elucidation of different physico-chemical parameters and the secretory enzymes released by Talaromyces verruculosus SGMNPf3 during cellulosic biomass degradation. METHODS AND RESULTS: We determined the optimal pH, temperature and time course parameters for the efficient degradation of different natural and commercial cellulosic substrates by T. verruculosus SGMNPf3, previously isolated from a forest soil. The optimal growth of the fungus and production of its cellulases were obtained when the culture condition was maintained at pH 3·3 and temperature 30°C. Activity of the crude cellulases was maximum at 60°C. Activity of cellulase enzymes produced on natural cellulose substrates was higher than that on commercial cellulose substrates. A continuous increase in cellulase activity at different time points indicated no apparent end product inhibition. This might be attributed to the high individual cellulases, notably β-glucosidase (316·1 μmol g(-1) ) production. Zymogram of extracellular crude proteins showed two dominant extracellular protein bands of molecular weight 72·3 and 61·4 kDa, indicating their cellulolytic nature. MALDI-TOF and LC-MS/MS analysis of the 2DE spots also identified several enzymes including β-glucosidase involved in the process of cellulose degradation. CONCLUSIONS: Based on its optimal parameters for cellulolytic activities, we suggest that the fungus is acido-mesophilic. There was apparently no end-product inhibition of the cellulase activity and this is attributed to the ability of the fungus to produce sufficient β-glucosidase. The dominant proteins secreted by the fungus were confirmed to be cellulases. SIGNIFICANCE AND IMPACT OF THE STUDY: The high individual cellulase activities, better cellulase production on natural substrates and apparent absence of end-product inhibition are characteristics of T. verruculosus SGMNPf3 for use in harvesting naturally endowed energy in cellulosic biomass