Ag2ZnSnS4 Nanocrystals Expand the Availability of RoHS Compliant Colloidal Quantum Dots

The demonstration of the quantum confinement effect in colloidal quantum dots (QDs) has been extensively studied and exploited mainly in Pb and Cd chalcogenide systems. There has been an urgent need recently for the development of non(less)-toxic colloidal QDs to warrant compliance with current safety regulations (Restriction of Hazardous Substances (RoHS) Directive 2002/95/EC). Herein, we report Pb/Cd-free, solution processed luminescent Ag2ZnSnS4 (AZTS) colloidal QDs. We present a selective and controlled amine and thiol-free synthesis of air stable luminescent AZTS QDs by the hot injection technique. By controlling the reaction conditions we obtain controlled size variation and demonstrate the quantum confinement effect that is in good agreement with the theoretically calculated values. The band gap of the AZTS QDs is size-tunable in the near-infrared from 740 to 850 nm. Finally, we passivate the surface with Zn-oleate, which yields higher quantum yield (QY), longer lifetime, and better colloidal stability.Peer ReviewedPostprint (published version

A VALIDATED STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF AVAPRITINIB IN BULK AND TABLET DOSAGE FORM

Objective: The present investigation aims to develop a simple, cost effective, and reliable stability-indicating RP-HPLC assay method for analysis of avapritinib in bulk and the tablet dosage form. Methods: The method was developed using Acetonitrile and Methanol in a ratio of 70:30 (v/v) as the mobile phase, flowing at a rate of 1 ml/min, in an isocratic mode. A reverse phase column (Symmetry C18 column, 250 mm x 4.6 mm, 5 µm) was used as the stationary phase. The detection of the compound was made using a UV detector set at 245 nm. The validation and force degradation studies of the method were done following the International Conference on Harmonization (ICH) guidelines. Results: The method was validated using the prescribed parameters like system suitability, LOD, LOQ, accuracy, precision, robustness, specificity etc. The relative standard deviation (% RSD) of the peak area observed in each case was found within the accepted range (< 2%). The tailing factor and the plate count were found to be less than 2 and more than 2000 respectively in each observation.  The coefficient of correlation (r2) value in the linearity study was found to be 0.9997. The drug was found to be quantified accurately in presence of its degraded products. Conclusion: The developed simple and economic method is a suitable option for the qualitative and quantitative study of avapritinib in bulk and tablets even in presence of its degraded products which may arise because of oxidation, hydrolysis, thermal and photolytic decomposition

Free fatty acids regulating action of Capparis decidua fruit on dyslipidemia in rats

Capparis decidua belongs to family Capparidaceae in wastelands of India. The study aim was to determine the role of C. decidua fruits on the free fatty acids (FFA) profile in fat-rich diet (FRD) dyslipidemic rats. The methanolic extract of edible fruit of C. decidua (CD) was given orally to obese dyslipidemic rats at the dose of 125 mg/kg and 250 mg/kg for consecutive 28 days. CD treatment in FRD rats significantly restricts the body weight gains. Blood lipid profile was altered dose dependently and significantly after 4-week treatment with CD to FRD. rats. It significantly (p<0.05) enhanced serum FFA especially, g-linolinate, a-linolinate, arachidonate, ecosapentaenoate, docosapentaenoate and docosahexaenoate. Moreover, w3-PUFA content was also enhanced (50.3% and 78.8%) in the serum of CD treated animals, whereas MUFA was lowered (31.1% and 40%). Therefore, Capparis decidua fruit has a promising role on dyslipidemia and obesity and has the capabilities to regulate beneficial free fatty acids.

A comparative study of fatty acids profile of two Indian major carps (Gibelion catla, Hamilton, 1822 and Cirrhinus mrigala, Hamilton, 1822) using value added fish feed

Freshwater fishes are not only a major source of protein but they also possess nutritionally valuable lipids in the form of Polyunsaturated fatty acids (PUFAs), which play a crucial role in the normal growth, disease prevention, development, cardiovascular health and reproduction of human. The present study was performed to determine the incorporation rate of fatty acids profile and their composition in two common freshwater carps as Gibelion catla and Cirrhinus mrigala (in situ trial and experimental) in the different experimental time period (0 days, i.e. initial, 90 days and 180 days) by using of value added feed like flaxseed (?-linolenic acids, 51.26% – 54.94%) and soybean oil (?-linolenic acids, 7.95%-9.01%) as omega-3 supplements. To determine the specific growth pattern Length-Weight Relationships (LWRs) are analyzed where it showed positive allometric growth (b=3.20 in 90 days, b=3.11 in 180 days for Catla and b=3.18 in 90 days, b=3.1 in 180 days for Mrigala fish). The Gas Chromatography-Mass Spectrometry (GC/MS) method also confirmed that the percentages of EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) increased significantly (P< 0.05) in experimental (0.096a±0.41, initial; 5.16a±0.27, 90 days; 6.21b±0.36, 180 days Catla fish species and 0.019a±0.96 initial; 3.74b±0.37, 90 days; 3.50a±0.46 180 days for Mrigal fish species) fishes rather than controls (4.28a±0.27, 90 days; 4.36b±0.36, 180 days for Catla species and 2.24b±0.31 90 days; 2.50a±0.11 180 days for Mrigal species). Therefore, it was clearly indicated that formulated diet performed significantly to maintain the positive allometric growth as well as successive enrichment of PUFAs in experimental specimens, which is beneficial for human health as high source of protein and PUFAs as well

Knowledge, attitudes, and practices regarding malaria control among the slash and burn cultivators in Rangamati Hill tracts of Bangladesh

Background: Slash and burn cultivators are a significant risk group for malaria in South-East Asia. As envisaged in the National Strategic Plan for Malaria Elimination, Bangladesh aims to achieve zero indigenous malaria transmission by 2030. For the national plan to move from malaria control to malaria elimination, targeting the population of slash and burn cultivators is of overriding importance. Methods: The study used an explorative mixed method design to investigate the knowledge, attitudes, and practices (KAP) regarding malaria prevention and treatment in an endemic area of Bangladesh. Adult slash and burn cultivators in two sub-districts of the Rangamati District were selected and interviewed. Four focus group discussions were conducted, and this was followed by a cross-sectional quantitative survey with 200 participants. Results: The respondents’ general knowledge about malaria transmission and modes of prevention and treatment was good. However, there were some gaps regarding knowledge about specific aspects of malaria transmission and in particular about the increased risk associated with their occupation. Despite a much-reduced incidence of malaria in the study area, the respondents perceived the disease as life-threatening and knew that it needs rapid attention from a health worker. Moreover, the specific services offered by the local community health workers for malaria diagnosis and treatment were highly appreciated. Finally, the use of insecticide-treated mosquito nets (ITN) was considered as important and this intervention was uniformly stated as the main malaria prevention method. Conclusions: The findings from this study on promising KAP characteristics in the slash and burn cultivator population are reassuring that the goal of malaria elimination by the year 2030 can be achieved in Bangladesh

Proof of Mirror Theory for a Wide Range of ξmax⁡\xi_{\max}

In CRYPTO\u2703, Patarin conjectured a lower bound on the number of distinct solutions $(P_1, \ldots, P_{q}) \in (\{0, 1\}^{n})^{q}$ satisfying a system of equations of the form $X_i \oplus X_j = \lambda_{i,j}$ such that $P_1, P_2, \ldots$, $P_{q}$ are pairwise distinct. This result is known as \emph{``$P_i \oplus P_j$ Theorem for any $\xi_{\max}$\u27\u27} or alternatively as \emph{Mirror Theory for general $\xi_{\max}$}, which was later proved by Patarin in ICISC\u2705. Mirror theory for general $\xi_{\max}$ stands as a powerful tool to provide a high-security guarantee for many blockcipher-(or even ideal permutation-) based designs. Unfortunately, the proof of the result contains gaps that are non-trivial to fix. In this work, we present the first complete proof of the $P_i \oplus P_j$ theorem for a wide range of $\xi_{\max}$, typically up to order $O(2^{n/4}/\sqrt{n})$. Furthermore, our proof approach is made simpler by using a new type of equation, dubbed link-deletion equation, that roughly corresponds to half of the so-called orange equations from earlier works. As an illustration of our result, we also revisit the security proofs of two optimally secure blockcipher-based pseudorandom functions, and $n$-bit security proof for six round Feistel cipher, and provide updated security bounds

Malabaricone-A Induces A Redox Imbalance That Mediates Apoptosis in U937 Cell Line

BACKGROUND: The 'two-faced' character of reactive oxygen species (ROS) plays an important role in cancer biology by acting both as secondary messengers in intracellular signaling cascades and sustaining the oncogenic phenotype of cancer cells, while on the other hand, it triggers an oxidative assault that causes a redox imbalance translating into an apoptotic cell death. PRINCIPAL FINDINGS: Using a tetrazolium [{3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl}-2H-tetrazolium] based cell viability assay, we evaluated the cytotoxicity of a plant derived diarylnonanoid, malabaricone-A on leukemic cell lines U937 and MOLT-3. This cytotoxicity hinged on its ability to cause a redox imbalance via its ability to increase ROS, measured by flow cytometry using 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate and by decreasing glutathione peroxidase activity. This redox imbalance mediated apoptosis was evident by an increase in cytosolic [Ca(2+)], externalization of phosphatidyl serine as also depolarization of the mitochondrial membrane potential as measured by flow cytometry. There was concomitant peroxidation of cardiolipin, release of free cytochrome c to cytosol along with activation of caspases 9, 8 and 3. This led to cleavage of the DNA repair enzyme, poly (ADP-ribose) polymerase that caused DNA damage as proved by labeling with 4',6-diamidino-2-phenylindole (DAPI); furthermore, terminal deoxy ribonucleotide transferase catalysed incorporation of deoxy uridine triphosphate confirmed DNA nicking and was accompanied by arrest of cell cycle progression. CONCLUSIONS: Taken together, compounds like MAL-A having pro-oxidant activity mediate their cytotoxicity in leukemic cells via induction of oxidative stress triggering a caspase dependent apoptosis

Free fatty acids regulating action of Capparis decidua fruit on dyslipidemia in rats

Capparis decidua belongs to family Capparidaceae in wastelands of India. The study aim was to determine the role of C. decidua fruits on the free fatty acids (FFA) profile in fat-rich diet (FRD) dyslipidemic rats. The methanolic extract of edible fruit of C. decidua (CD) was given orally to obese dyslipidemic rats at the dose of 125 mg/kg and 250 mg/kg for consecutive 28 days. CD treatment in FRD rats significantly restricts the body weight gains. Blood lipid profile was altered dose dependently and significantly after 4-week treatment with CD to FRD. rats. It significantly (p<0.05) enhanced serum FFA especially, -linolinate, -linolinate, arachidonate, ecosapentaenoate, docosapentaenoate and docosahexaenoate. Moreover, 3-PUFA content was also enhanced (50.3% and 78.8%) in the serum of CD treated animals, whereas MUFA was lowered (31.1% and 40%). Therefore, Capparis decidua fruit has a promising role on dyslipidemia and obesity and has the capabilities to regulate beneficial free fatty acids