The Expression and Localization of N-Myc Downstream-Regulated Gene 1 in Human Trophoblasts

The protein N-Myc downstream-regulated gene 1 (NDRG1) is implicated in the regulation of cell proliferation, differentiation, and cellular stress response. NDRG1 is expressed in primary human trophoblasts, where it promotes cell viability and resistance to hypoxic injury. The mechanism of action of NDRG1 remains unknown. To gain further insight into the intracellular action of NDRG1, we analyzed the expression pattern and cellular localization of endogenous NDRG1 and transfected Myc-tagged NDRG1 in human trophoblasts exposed to diverse injuries. In standard conditions, NDRG1 was diffusely expressed in the cytoplasm at a low level. Hypoxia or the hypoxia mimetic cobalt chloride, but not serum deprivation, ultraviolet (UV) light, or ionizing radiation, induced the expression of NDRG1 in human trophoblasts and the redistribution of NDRG1 into the nucleus and cytoplasmic membranes associated with the endoplasmic reticulum (ER) and microtubules. Mutation of the phosphopantetheine attachment site (PPAS) within NDRG1 abrogated this pattern of redistribution. Our results shed new light on the impact of cell injury on NDRG1 expression patterns, and suggest that the PPAS domain plays a key role in NDRG1's subcellular distribution. © 2013 Shi et al

CASTOR: Centauro and Strange Object Research in nucleus-nucleus collisions at LHC

We describe the CASTOR detector designed to probe the very forward, baryon-rich rapidity region in nucleus-nucleus collisions at the LHC. We present a phenomenological model describing the formation of a QGP fireball in a high baryochemical potential environment, and its subsequent decay into baryons and strangelets. The model explains Centauros and the long-penetrating component and makes predictions for the LHC. Simulations of Centauro-type events were done. To study the response of the apparatus to new effects different exotic species (DCC, Centauros, strangelets etc.) were passed through the deep calorimeter. The energy deposition pattern in the calorimeter appears to be a new clear signature of the QGP.Comment: Talk given by E. Gladysz-Dziadus for the CASTOR group, Intern. Workshop on Nuclear Theory, 10-15 June, 2002, Bulgaria, Rila Mountains, 15 pages, 14 figure

Vulnerability of primitive human placental trophoblast to Zika virus

Infection of pregnant women by Asian lineage strains of Zika virus (ZIKV) has been linked to brain abnormalities in their infants, yet it is uncertain when during pregnancy the human conceptus is most vulnerable to the virus. We have examined two models to study susceptibility of human placental trophoblast to ZIKV: cytotrophoblast and syncytiotrophoblast derived from placental villi at term and colonies of trophoblast differentiated from embryonic stem cells (ESC). The latter appear to be analogous to the primitive placenta formed during implantation. The cells from term placentas, which resist infection, do not express genes encoding most attachment factors implicated in ZIKV entry but do express many genes associated with antiviral defense. By contrast, the ESC-derived trophoblasts possess a wide range of attachment factors for ZIKV entry and lack components of a robust antiviral response system. These cells, particularly areas of syncytiotrophoblast within the colonies, quickly become infected, produce infectious virus and undergo lysis within 48 h after exposure to low titers (multiplicity of infection > 0.07) of an African lineage strain (MR766 Uganda: ZIKVU) considered to be benign with regards to effects on fetal development. Unexpectedly, lytic effects required significantly higher titers of the presumed more virulent FSS13025 Cambodia (ZIKVC). Our data suggest that the developing fetus might be most vulnerable to ZIKV early in the first trimester before a protective zone of mature villous trophoblast has been established. Additionally, MR766 is highly trophic toward primitive trophoblast, which may put the early conceptus of an infected mother at high risk for destruction

JUNO Conceptual Design Report

The Jiangmen Underground Neutrino Observatory (JUNO) is proposed to determine the neutrino mass hierarchy using an underground liquid scintillator detector. It is located 53 km away from both Yangjiang and Taishan Nuclear Power Plants in Guangdong, China. The experimental hall, spanning more than 50 meters, is under a granite mountain of over 700 m overburden. Within six years of running, the detection of reactor antineutrinos can resolve the neutrino mass hierarchy at a confidence level of 3-4$\sigma$, and determine neutrino oscillation parameters $\sin^2\theta_{12}$, $\Delta m^2_{21}$, and $|\Delta m^2_{ee}|$ to an accuracy of better than 1%. The JUNO detector can be also used to study terrestrial and extra-terrestrial neutrinos and new physics beyond the Standard Model. The central detector contains 20,000 tons liquid scintillator with an acrylic sphere of 35 m in diameter. $\sim$17,000 508-mm diameter PMTs with high quantum efficiency provide $\sim$75% optical coverage. The current choice of the liquid scintillator is: linear alkyl benzene (LAB) as the solvent, plus PPO as the scintillation fluor and a wavelength-shifter (Bis-MSB). The number of detected photoelectrons per MeV is larger than 1,100 and the energy resolution is expected to be 3% at 1 MeV. The calibration system is designed to deploy multiple sources to cover the entire energy range of reactor antineutrinos, and to achieve a full-volume position coverage inside the detector. The veto system is used for muon detection, muon induced background study and reduction. It consists of a Water Cherenkov detector and a Top Tracker system. The readout system, the detector control system and the offline system insure efficient and stable data acquisition and processing.Comment: 328 pages, 211 figure

Deep sub-threshold Ξ−\Xi^- production in Ar+KCl reactions at 1.76A GeV

We report first results on a deep sub-threshold production of the doubly strange hyperon $\Xi^-$ in a heavy-ion reaction. At a beam energy of 1.76A GeV the reaction Ar+KCl was studied with the High Acceptance Di-Electron Spectrometer (HADES) at SIS18/GSI. A high-statistics and high-purity $\Lambda$ sample was collected, allowing for the investigation of the decay channel $\Xi^- \to \Lambda \pi^-$. The deduced $\Xi^-/(\Lambda+\Sigma^0)$ production ratio of $(5.6 \pm 1.2 ^{+1.8}_{-1.7})\cdot 10^{-3}$ is significantly larger than available model predictions.Comment: 4 pages, including 4 figure

Lambda hyperon production and polarization in collisions of p(3.5 GeV) + Nb

Results on $\Lambda$ hyperon production are reported for collisions of p(3.5 GeV) + Nb, studied with the High Acceptance Di-Electron Spectrometer (HADES) at SIS18 at GSI Helmholtzzentrum for Heavy-Ion Research, Darmstadt. The transverse mass distributions in rapidity bins are well described by Boltzmann shapes with a maximum inverse slope parameter of about $90\,$MeV at a rapidity of $y=1.0$, i.e. slightly below the center-of-mass rapidity for nucleon-nucleon collisions, $y_{cm}=1.12$. The rapidity density decreases monotonically with increasing rapidity within a rapidity window ranging from 0.3 to 1.3. The $\Lambda$ phase-space distribution is compared with results of other experiments and with predictions of two transport approaches which are available publicly. None of the present versions of the employed models is able to fully reproduce the experimental distributions, i.e. in absolute yield and in shape. Presumably, this finding results from an insufficient modelling in the transport models of the elementary processes being relevant for $\Lambda$ production, rescattering and absorption. The present high-statistics data allow for a genuine two-dimensional investigation as a function of phase space of the self-analyzing $\Lambda$ polarization in the weak decay $\Lambda\rightarrow p \pi^-$. Finite negative values of the polarization in the order of $5-20\,\%$ are observed over the entire phase space studied. The absolute value of the polarization increases almost linearly with increasing transverse momentum for $p_t>300\,$MeV/c and increases with decreasing rapidity for $y < 0.8$.Comment: accepted by Eur. Phys. J.

Subthreshold Xi- Production in Collisions of p(3.5 GeV)+Nb

Results on the production of the double-strange cascade hyperon $\mathrm{\Xi^-}$ are reported for collisions of p\,(3.5~GeV)\,+\,Nb, studied with the High Acceptance Di-Electron Spectrometer (HADES) at SIS18 at GSI Helmholtzzentrum for Heavy-Ion Research, Darmstadt. For the first time, subthreshold $\mathrm{\Xi^-}$ production is observed in proton-nucleus interactions. Assuming a $\mathrm{\Xi^-}$ phase-space distribution similar to that of $\mathrm{\Lambda}$ hyperons, the production probability amounts to $P_{\mathrm{\Xi^-}}=(2.0\,\pm0.4\,\mathrm{(stat)}\,\pm 0.3\,\mathrm{(norm)}\,\pm 0.6\,\mathrm{(syst)})\times10^{-4}$ resulting in a $\mathrm{\Xi^-/(\Lambda+\Sigma^0)}$ ratio of $P_{\mathrm{\Xi^-}}/\ P_{\mathrm{\Lambda+\Sigma^0}}=(1.2\pm 0.3\,\mathrm{(stat)}\pm0.4\,\mathrm{(syst)})\times10^{-2}$. Available model predictions are significantly lower than the estimated$\mathrm{\Xi^-}$ yield.Comment: 6 pages, 3 figure

In-Medium Effects on K0 Mesons in Relativistic Heavy-Ion Collisions

We present the transverse momentum spectra and rapidity distributions of $\pi^{-}$ and K$^0_S$ in Ar+KCl reactions at a beam kinetic energy of 1.756 A GeV measured with the spectrometer HADES. The reconstructed K$^0_S$ sample is characterized by good event statistics for a wide range in momentum and rapidity. We compare the experimental $\pi^{-}$ and K$^0_S$ distributions to predictions by the IQMD model. The model calculations show that K$^0_S$ at low tranverse momenta constitute a particularly well suited tool to investigate the kaon in-medium potential. Our K$^0_S$ data suggest a strong repulsive in-medium K$^0$ potential of about 40 MeV strength.Comment: 10 pages, 10 figures, accepted by Phys. Rev.

Baryonic resonances close to the Kbar-N threshold: the case of Sigma(1385)^+ in pp collisions

We present results of an exclusive measurement of the first excited state of the Sigma hyperon, Sigma(1385)^+, produced in p+p -> Sigma^+ + K^+ + n at 3.5 GeV beam energy. The extracted data allow to study in detail the invariant mass distribution of the Sigma(1385)^+. The mass distribution is well described by a relativistic Breit-Wigner function with a maximum at m_0 = 1383.2 +- 0.9 MeV/c^2 and a width of 40.2 +- 2.1 MeV/c^2. The exclusive production cross-section comes out to be 22.27 +- 0.89 +- 1.56 +3.07 -2.10 mu b. Angular distributions of the Sigma(1385)^+ in different reference frames are found to be compatible with the hypothesis that 33 % of Sigma(1385)^+ result from the decay of an intermediate Delta^{++} resonance.Comment: 12 pages; 12 figures; submitted to PR