A new flat shell finite element for the linear analysis of thin shell structures

In this paper, a new rectangular flat shell element denoted ‘ACM_RSBE5’ is presented. The new element is obtained by superposition of the new strain-based membrane element ‘RSBE5’ and the well-known plate bending element ‘ACM’. The element can be used for the analysis of any type of thin shell structures, even if the geometry is irregular. Comparison with other types of shell elements is performed using a series of standard test problems. A correlation study with an experimentally tested aluminium shell is also conducted. The new shell element proved to have a fast rate of convergence and to provide accurate results

Coexistence and Criticality in Size-Asymmetric Hard-Core Electrolytes

Liquid-vapor coexistence curves and critical parameters for hard-core 1:1 electrolyte models with diameter ratios lambda = sigma_{-}/\sigma_{+}=1 to 5.7 have been studied by fine-discretization Monte Carlo methods. Normalizing via the length scale sigma_{+-}=(sigma_{+} + sigma_{-})/2 relevant for the low densities in question, both Tc* (=kB Tc sigma_{+-}/q^2 and rhoc* (= rhoc sigma _{+-}^{3}) decrease rapidly (from ~ 0.05 to 0.03 and 0.08 to 0.04, respectively) as lambda increases. These trends, which unequivocally contradict current theories, are closely mirrored by results for tightly tethered dipolar dimers (with Tc* lower by ~ 0-11% and rhoc* greater by 37-12%).Comment: 4 pages, 5 figure

Reptile search algorithm (RSA)-based selective harmonic elimination technique in Packed E-Cell (PEC-9) inverter

The multilevel inverters (MLIs) are capable of handling large quantities of power and generating high-quality output voltages. Consequently, the size of the filters is reduced, and the circuitry is simplified. As a result, they have a diverse range of uses in the industrial sector, especially in smart grids. The input voltage boosting feature is required to utilize the MLI with renewable energy. In addition, a large number of components are required to attain higher output voltage levels, which increases the cost of the circuit and weight. A variety of MLI topologies have been identified to reduce losses, device quantity, and device ratings. The selective harmonic elimination (SHE) approaches reduce distinct lower order harmonics by computing the ideal switching angles. This research presents a nine–level Packed E–Cell (PEC–9) inverter that uses selective harmonic elimination to eliminate total harmonic distortion. In order to calculate the best switching angle, the reptile search algorithm (RSA) is implemented in this paper, a nature–inspired metaheuristic algorithm inspired by the hunting behavior of the crocodile. The hunting behavior of crocodiles is implemented in two main steps: the first is encircling, which is accomplished by belly walking or high walking, and the second is hunting, which is accomplished by hunting cooperation or hunting coordination. In this technique, nonlinear transcendental equations have been solved. The simulation was run in the MATLAB R2021b software environment. The simulation results suggest that the RSA outperforms the other metaheuristic algorithms. Furthermore, the simulation result was validated on a hardware setup using DSP–TMS320F28379D in the laboratory

Risk Factors for Severe Renal Disease in Bardet-Biedl Syndrome

Bardet-Biedl syndrome is a rare autosomal recessive, multisystem disease characterized by retinal dystrophy, renal malformation, obesity, intellectual disability, polydactyly, and hypogonadism. Nineteen disease-causing genes (BBS1-19) have been identified, of which mutations in BBS1 are most common in North America and Europe. A hallmark of the disease, renal malformation is heterogeneous and is a cause of morbidity and mortality through the development of CKD. We studied the prevalence and severity of CKD in 350 patients with Bardet-Biedl syndrome-related renal disease attending the United Kingdom national Bardet-Biedl syndrome clinics to further elucidate the phenotype and identify risk indicators of CKD. Overall, 31% of children and 42% of adults had CKD; 6% of children and 8% of adults had stage 4-5 CKD. In children, renal disease was often detected within the first year of life. Analysis of the most commonly mutated disease-associated genes revealed that, compared with two truncating mutations, two missense mutations associated with less severe CKD in adults. Moreover, compared with mutations in BBS10, mutations in BBS1 associated with less severe CKD or lack of CKD in adults. Finally, 51% of patients with available ultrasounds had structural renal abnormalities, and 35% of adults were hypertensive. The presence of structural abnormalities or antihypertensive medication also correlated statistically with stage 3b-5 CKD. This study describes the largest reported cohort of patients with renal disease in Bardet-Biedl syndrome and identifies risk factors to be considered in genetic counseling

Thermodynamics of Electrolytes on Anisotropic Lattices

The phase behavior of ionic fluids on simple cubic and tetragonal (anisotropic) lattices has been studied by grand canonical Monte Carlo simulations. Systems with both the true lattice Coulombic potential and continuous-space $1/r$ electrostatic interactions have been investigated. At all degrees of anisotropy, only coexistence between a disordered low-density phase and an ordered high-density phase with the structure similar to ionic crystal was found, in contrast to recent theoretical predictions. Tricritical parameters were determined to be monotonously increasing functions of anisotropy parameters which is consistent with theoretical calculations based on the Debye-H\"uckel approach. At large anisotropies a two-dimensional-like behavior is observed, from which we estimated the dimensionless tricritical temperature and density for the two-dimensional square lattice electrolyte to be $T^*_{tri}=0.14$ and $\rho^*_{tri} = 0.70$.Comment: submitted to PR

Integrated transcriptomic and proteomic analysis of pathogenic mycobacteria and their esx-1 mutants reveal secretion-dependent regulation of ESX-1 substrates and WhiB6 as a transcriptional regulator

The mycobacterial type VII secretion system ESX-1 is responsible for the secretion of a number of proteins that play important roles during host infection. The regulation of the expression of secreted proteins is often essential to establish successful infection. Using transcriptome sequencing, we found that the abrogation of ESX-1 function in Mycobacterium marinum leads to a pronounced increase in gene expression levels of the espA operon during the infection of macrophages. In addition, the disruption of ESX-1-mediated protein secretion also leads to a specific down-regulation of the ESX-1 substrates, but not of the structural components of this system, during growth in culture medium. This effect is observed in both M. marinum and M. tuberculosis. We established that down-regulation of ESX-1 substrates is the result of a regulatory process that is influenced by the putative transcriptional regulator whib6, which is located adjacent to the esx-1 locus. In addition, the overexpression of the ESX-1-associated PE35/PPE68 protein pair resulted in a significantly increased secretion of the ESX-1 substrate EsxA, demonstrating a functional link between these proteins. Taken together, these data show that WhiB6 is required for the secretion-dependent regulation of ESX-1 substrates and that ESX-1 substrates are regulated independently from the structural components, both during infection and as a result of active secretion

Rational measures of agromelioration on grey soils

In the conditions of grey soils in the south of Kazakhstan, the soil is degraded due to irrational use of irrigated land and non-compliance with agro-reclamation works in its order, as well as excessive irrigation of medium-saline soil, it negatively affects crop yields. In addition, on medium-saline soils, winter plowing is carried out only to a shallow depth of 30-32 cm. Mainly on grey soils, with timely and intensive soil treatment, it is preferable, since the degree of bulk mass of gray soils in a stable indicator is 1.28-1.30 g/cm3. If agrotechnological measures are carried out incorrectly, i.e. it affects the increase in soil density and its salinization, which negatively affects the growth and productivity of agricultural crops. In conditions of decreasing soil fertility, scientists of our scientific institution have carried out experimental work to identify the effects of effective agro-reclamation measures on irrigated saline lands, based on the rational use of irrigated agriculture in the south of Kazakhstan. The first issue that hinders the development of agricultural crops and the regulation of the condition of the rooting layer of plants in the soil is soil salinization and measures to combat them, since this issue is very relevant in conditions of increasing irrigated agriculture intensity

Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations.

Although Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor contributing to this variability may be the diversity of the BCG strains that are used around the world, in part from genomic changes accumulated during vaccine production and their resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression of proteins across five representative BCG strains of the four tandem duplication (DU) groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and insertions and deletions (indels) across fourteen BCG strains. Genome-wide SNP characterization allowed the construction of a new and robust phylogenic genealogy of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may be reflected by altered immunogenicity and possibly vaccine efficacy. Together, these integrated-omic data represent the most comprehensive catalogue of genetic variation across a global collection of BCG strains

Born radicals? Prevent, positivism, and ‘race-thinking’

In the contemporary Western climate, counter-terrorism discourse dealing with so-called Islamic extremism appears to be obsessed with trying to understand the motives behind what prompts somebody to turn to terrorism. This paper will argue that attempts to locate extremist motives in such a way can be seen to reinforce earlier iterations of positivist criminology and race-thinking. Through a critical examination of the works of criminologist Cesare Lombroso, this paper will tease out the interconnections between his ‘criminal types’ thesis, and the British government’s current Prevent policy that seeks to identify ‘extremist types.’ By developing a rich critique of these positivist approaches, the paper will go on to question how we might think beyond the essentialism, reductivism, and racism/Islamophobia inherent within such frameworks. In this way, the paper raises a series of conceptual implications for criminology and terrorism studies, while at the same time, develops a contribution to critical race and ethnicity studies