3,020 research outputs found
Perfect Fluid Quantum Anisotropic Universe: Merits and Challenges
The present paper deals with quantization of perfect fluid anisotropic
cosmological models. Bianchi type V and IX models are discussed following
Schutz's method of expressing fluid velocities in terms of six potentials. The
wave functions are found for several examples of equations of state. In one
case a complete wave packet could be formed analytically. The initial
singularity of a zero proper volume can be avoided in this case, but it is
plagued by the usual problem of non-unitarity of anisotropic quantum
cosmological models. It is seen that a particular operator ordering alleviates
this problem.Comment: 13 pages, 4 figures; Accepted for publication in Gen Relativ Gravi
Gene silencing in tick cell lines using small interfering or long double-stranded RNA
Gene silencing by RNA interference (RNAi) is an important research tool in many areas of biology. To effectively harness the power of this technique in order to explore tick functional genomics and tick-microorganism interactions, optimised parameters for RNAi-mediated gene silencing in tick cells need to be established. Ten cell lines from four economically important ixodid tick genera (Amblyomma, Hyalomma, Ixodes and Rhipicephalus including the sub-species Boophilus) were used to examine key parameters including small interfering RNA (siRNA), double stranded RNA (dsRNA), transfection reagent and incubation time for silencing virus reporter and endogenous tick genes. Transfection reagents were essential for the uptake of siRNA whereas long dsRNA alone was taken up by most tick cell lines. Significant virus reporter protein knockdown was achieved using either siRNA or dsRNA in all the cell lines tested. Optimum conditions varied according to the cell line. Consistency between replicates and duration of incubation with dsRNA were addressed for two Ixodes scapularis cell lines; IDE8 supported more consistent and effective silencing of the endogenous gene subolesin than ISE6, and highly significant knockdown of the endogenous gene 2I1F6 in IDE8 cells was achieved within 48 h incubation with dsRNA. In summary, this study shows that gene silencing by RNAi in tick cell lines is generally more efficient with dsRNA than with siRNA but results vary between cell lines and optimal parameters need to be determined for each experimental system
Combination schemes for turning point prediction
We propose new forecast combination schemes for predicting turning points of business cycles. The combination schemes deal with the forecasting performance of a given set of models and possibly providing better turning point predictions. We consider turning point predictions generated by autoregressive (AR) and Markov-Switching AR models, which are commonly used for business cycle analysis. In order to account for parameter uncertainty we consider a Bayesian approach to both estimation and prediction and compare, in terms of statistical accuracy, the individual models and the combined turning point predictions for the United States and Euro area business cycles
The characteristic blue spectra of accretion disks in quasars as uncovered in the infrared
Quasars are thought to be powered by supermassive black holes accreting
surrounding gas. Central to this picture is a putative accretion disk which is
believed to be the source of the majority of the radiative output. It is well
known, however, that the most extensively studied disk model -- an optically
thick disk which is heated locally by the dissipation of gravitational binding
energy -- is apparently contradicted by observations in a few major respects.
In particular, the model predicts a specific blue spectral shape asymptotically
from the visible to the near-infrared, but this is not generally seen in the
visible wavelength region where the disk spectrum is observable. A crucial
difficulty was that, toward the infrared, the disk spectrum starts to be hidden
under strong hot dust emission from much larger but hitherto unresolved scales,
and thus has essentially been impossible to observe. Here we report
observations of polarized light interior to the dust-emiting region that enable
us to uncover this near-infrared disk spectrum in several quasars. The revealed
spectra show that the near-infrared disk spectrum is indeed as blue as
predicted. This indicates that, at least for the outer near-infrared-emitting
radii, the standard picture of the locally heated disk is approximately
correct. The model problems at shorter wavelengths should then be directed
toward a better understanding of the inner parts of the revealed disk. The
newly uncovered disk emission at large radii, with more future measurements,
will also shed totally new light on the unanswered critical question of how and
where the disk ends.Comment: published in Nature, 24 July 2008 issue. Supplementary Information
can be found at
http://www.mpifr-bonn.mpg.de/div/ir-interferometry/suppl_info.pdf Published
version can be accessed from
http://www.nature.com/nature/journal/v454/n7203/pdf/nature07114.pd
Characterizing and quantifying the effects of breast cancer therapy using mathematical modeling
We designed a mathematical model to describe and quantify the mechanisms and dynamics of tumor growth, cell-kill and resistance as they affect durations of benefit after cancer treatment. Our aim was to explore how treatment efficacy may be related to primary tumor characteristics, with the potential to guide future trial design and appropriate selection of therapy. Assuming a log-normal distribution of both resistant disease and tumor doubling times generates disease-free survival (DFS) or invasive DFS curves with specific shapes. Using a multivariate mathematical model, both treatment and tumor characteristics are related to quantified resistant disease and tumor regrowth rates by allowing different mean values for the influence of different treatments or clinical subtypes on these two log-normal distributions. Application of the model to the CALGB 9741 adjuvant breast cancer trial showed that dose-dense therapy was estimated to achieve an extra 3/4 log of cell-kill compared to standard therapy, but only in patients with more rapidly growing ER-negative tumors. Application of the model to the AZURE trial of adjuvant bisphosphonate treatment suggested that the 5-year duration of zoledronic acid was adequate for ER-negative tumors, but may not be so for ER-positive cases, with increased recurrences after ceasing the intervention. Mathematical models can identify different effects of treatment by subgroup and may aid in treatment design, trial analysis, and appropriate selection of therapy. They may provide a more appropriate and insightful tool than the conventional Cox model for the statistical analysis of response durations
Persistence of magnetic field driven by relativistic electrons in a plasma
The onset and evolution of magnetic fields in laboratory and astrophysical
plasmas is determined by several mechanisms, including instabilities, dynamo
effects and ultra-high energy particle flows through gas, plasma and
interstellar-media. These processes are relevant over a wide range of
conditions, from cosmic ray acceleration and gamma ray bursts to nuclear fusion
in stars. The disparate temporal and spatial scales where each operates can be
reconciled by scaling parameters that enable to recreate astrophysical
conditions in the laboratory. Here we unveil a new mechanism by which the flow
of ultra-energetic particles can strongly magnetize the boundary between the
plasma and the non-ionized gas to magnetic fields up to 10-100 Tesla (micro
Tesla in astrophysical conditions). The physics is observed from the first
time-resolved large scale magnetic field measurements obtained in a laser
wakefield accelerator. Particle-in-cell simulations capturing the global plasma
and field dynamics over the full plasma length confirm the experimental
measurements. These results open new paths for the exploration and modelling of
ultra high energy particle driven magnetic field generation in the laboratory
DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose
Macroautophagy is a membrane-trafficking process that delivers cytoplasmic constituents to lysosomes for degradation. The process operates under basal conditions as a mechanism to turnover damaged or misfolded proteins and organelles. As a result, it has a major role in preserving cellular integrity and viability. In addition to this basal function, macroautophagy can also be modulated in response to various forms of cellular stress, and the rate and cargoes of macroautophagy can be tailored to facilitate appropriate cellular responses in particular situations. The macroautophagy machinery is regulated by a group of evolutionarily conserved autophagy-related (ATG) proteins and by several other autophagy regulators, which either have tissue-restricted expression or operate in specific contexts. We report here the characterization of a novel autophagy regulator that we have termed DRAM-3 due to its significant homology to damage-regulated autophagy modulator (DRAM-1). DRAM-3 is expressed in a broad spectrum of normal tissues and tumor cells, but different from DRAM-1, DRAM-3 is not induced by p53 or DNA-damaging agents. Immunofluorescence studies revealed that DRAM-3 localizes to lysosomes/autolysosomes, endosomes and the plasma membrane, but not the endoplasmic reticulum, phagophores, autophagosomes or Golgi, indicating significant overlap with DRAM-1 localization and with organelles associated with macroautophagy. In this regard, we further proceed to show that DRAM-3 expression causes accumulation of autophagosomes under basal conditions and enhances autophagic flux. Reciprocally, CRISPR/Cas9-mediated disruption of DRAM-3 impairs autophagic flux confirming that DRAM-3 is a modulator of macroautophagy. As macroautophagy can be cytoprotective under starvation conditions, we also tested whether DRAM-3 could promote survival on nutrient deprivation. This revealed that DRAM-3 can repress cell death and promote long-term clonogenic survival of cells grown in the absence of glucose. Interestingly, however, this effect is macroautophagy-independent. In summary, these findings constitute the primary characterization of DRAM-3 as a modulator of both macroautophagy and cell survival under starvation conditions
Facial expressions depicting compassionate and critical emotions: the development and validation of a new emotional face stimulus set
Attachment with altruistic others requires the ability to appropriately process affiliative and kind facial cues. Yet there is no stimulus set available to investigate such processes. Here, we developed a stimulus set depicting compassionate and critical facial expressions, and validated its effectiveness using well-established visual-probe methodology. In Study 1, 62 participants rated photographs of actors displaying compassionate/kind and critical faces on strength of emotion type. This produced a new stimulus set based on N = 31 actors, whose facial expressions were reliably distinguished as compassionate, critical and neutral. In Study 2, 70 participants completed a visual-probe task measuring attentional orientation to critical and compassionate/kind faces. This revealed that participants lower in self-criticism demonstrated enhanced attention to compassionate/kind faces whereas those higher in self-criticism showed no bias. To sum, the new stimulus set produced interpretable findings using visual-probe methodology and is the first to include higher order, complex positive affect displays
Author Correction: Bayesian reassessment of the epigenetic architecture of complex traits
The original version of this Article contains an error in Fig. 3 in which panel B was inadvertently duplicated from panel A. This has been corrected in both the PDF and HTML versions of the Article
Gravito-electromagnetic analogies
We reexamine and further develop different gravito-electromagnetic (GEM)
analogies found in the literature, and clarify the connection between them.
Special emphasis is placed in two exact physical analogies: the analogy based
on inertial fields from the so-called "1+3 formalism", and the analogy based on
tidal tensors. Both are reformulated, extended and generalized. We write in
both formalisms the Maxwell and the full exact Einstein field equations with
sources, plus the algebraic Bianchi identities, which are cast as the
source-free equations for the gravitational field. New results within each
approach are unveiled. The well known analogy between linearized gravity and
electromagnetism in Lorentz frames is obtained as a limiting case of the exact
ones. The formal analogies between the Maxwell and Weyl tensors are also
discussed, and, together with insight from the other approaches, used to
physically interpret gravitational radiation. The precise conditions under
which a similarity between gravity and electromagnetism occurs are discussed,
and we conclude by summarizing the main outcome of each approach.Comment: 60 pages, 2 figures. Improved version (compared to v2) with some
re-write, notation improvements and a new figure that match the published
version; expanded compared to the published version to include Secs. 2.3 and
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