Formation of propionate and butyrate by the human colonic microbiota

Acknowledgements The authors receive financial support from the Scottish Government Rural and Environmental Sciences and Analytical Services (RESAS). We would like to thank Sylvia Duncan for SCFA data of F. prausnitzii grown under different pH regimes and for critically reading the manuscript. The authors declare that they have no conflicts of interest.Peer reviewedPostprin

Experimental infection of sheep with ovine and bovine Dichelobacter nodosus isolates

AbstractThe aim of this study was, under experimental conditions, to investigate infection of Norwegian White sheep with ovine and bovine isolates of Dichelobacter nodosus of varying virulence. In addition, the efficacy of gamithromycin as a treatment for the experimentally induced infections was examined. The study was performed as a single foot inoculation using a boot. Four groups, each with six lambs, were inoculated with four different challenge strains (Group 1: benign bovine strain; Group 2: virulent bovine strain; Group 3: benign ovine strain; Group 4: virulent ovine strain). The main criterion to determine that infection was transferred was that D. nodosus isolate was obtained by culture. After the trial all lambs were treated with gamithromycin. Clinical symptoms of footrot developed in all groups, and when removing the boots two weeks after challenge, D. nodosus was isolated from 5 of 24 experimental lambs. All lambs tested negative for D. nodosus by PCR within six weeks after treatment with gamithromycin. This study strongly indicates that D. nodosus isolates from both sheep and cattle can be transferred to sheep under experimental conditions. The study also indicates that gamithromycin may be effective against D. nodosus

Stellar population synthesis at the resolution of 2003

We present a new model for computing the spectral evolution of stellar populations at ages between 100,000 yr and 20 Gyr at a resolution of 3 A across the whole wavelength range from 3200 to 9500 A for a wide range of metallicities. These predictions are based on a newly available library of observed stellar spectra. We also compute the spectral evolution across a larger wavelength range, from 91 A to 160 micron, at lower resolution. The model incorporates recent progress in stellar evolution theory and an observationally motivated prescription for thermally-pulsing stars on the asymptotic giant branch. The latter is supported by observations of surface brightness fluctuations in nearby stellar populations. We show that this model reproduces well the observed optical and near-infrared colour-magnitude diagrams of Galactic star clusters of various ages and metallicities. Stochastic fluctuations in the numbers of stars in different evolutionary phases can account for the full range of observed integrated colours of star clusters in the Magellanic Clouds. The model reproduces in detail typical galaxy spectra from the Early Data Release (EDR) of the Sloan Digital Sky Survey (SDSS). We exemplify how this type of spectral fit can constrain physical parameters such as the star formation history, metallicity and dust content of galaxies. Our model is the first to enable accurate studies of absorption-line strengths in galaxies containing stars over the full range of ages. Using the highest-quality spectra of the SDSS EDR, we show that this model can reproduce simultaneously the observed strengths of those Lick indices that do not depend strongly on element abundance ratios [abridged].Comment: 35 pages, 22 figures, to appear in MNRAS; version with full resolution figures available at http://www.iap.fr/~charlot/bc2003/pape

Titan ionospheric conductivities from Cassini measurements

International audienc

Immunoglobulin E suppression and cytokine modulation in mice orally tolerized to β-lactoglobulin

This study was designed to confirm the tolerogenic properties of β-lactoglobulin in a mouse model and to assess specific oral tolerance induction in humoral and cellular compartments. BALB/c mice were fed β-lactoglobulin (BLG) or whey proteins at different ages and subsequently intraperitoneally challenged 5 days later with both BLG and a non-specific antigen, ovalbumin (OVA). Three weeks later, oral tolerance induction was analysed in CMP-fed, versus saline-fed mice, by measuring specific seric and intestinal antibody responses, delayed-type hypersensitivity (DTH), specific splenocyte proliferation, and cytokine secretion patterns. Three-week-old mice fed high doses of either whey proteins or BLG (respectively 3 mg/g or 5 mg/g of body weight) were found to achieve oral tolerization. At humoral and mucosal levels, anti-BLG immunoglobulin E (IgE) were suppressed in these groups when compared with saline fed mice. With respect to cellular responses, systemic DTH and lymphocyte proliferation to BLG were also inhibited in CMP-fed mice. Weaning time was determined to be the best period for oral tolerance induction. Kinetic analyses showed however, that a minimum of 2 weeks was required for oral tolerance detection. Finally, cytokine profiles indicated a reciprocal decrease of interleukin-2 (IL-2) and interferon-γ (IFN-γ) versus an increase of IL-10 and transforming growth factor-β (TGF-β) secretions in tolerized mice. Taken together, these results clearly showed that oral administration of high doses of cows’ milk proteins can induce significant hyposensitization in mice, in a specific inhibition of T helper 1 (Th1) lymphocytes with the participation of suppressor cytokines