Yesus Kristus Sebagai Wahyu Terakhir Allah Dalam Konteks Kitab Ibrani 1:1-4

Penelitian ini berusaha menjawab tentang sosok Yesus Kristus yang diyakini orang-orang percaya (Kristen) tentang iman mereka yang meyakini seutuhnya bahwa Yesus Kristus (Sang Logos) itu adalah wahyu terakhir Allah, yang mana sebelumnya di dalam kitab-kitab Perjanjian Lama, Allah telah berbicara kepada umat-Nya lewat perantaraan nabi-nabi-Nya kepada Israel. Dan di zaman akhir inilah, Allah berbicara lewat perantaraan Anak-Nya yang diutus ke dalam dunia untuk menggenapi seluruh kitab Perjanjian Lama. Maksud tujuan Yesus Kristus sebagai sebuah penggenapan dari karya Allah untuk keselamatan umat manusia dari kengerian kekal Di dalam sejarah keselamatan manusia, sejak manusia pertama yang diciptakan TUHAN jatuh ke dalam dosa, manusia seharusnya menerima upahnya yaitu mati kekal karena pelanggaran atau ketidaktaatan Adam dan Hawa saat itu (Kejadian 2:17). Tetapi inisiatif Allahlah yang merencanakan keselamatan bagi manusia hingga saat ini (Kejadian 3:21).  Penggenapan itu dengan mengutus Yesus Kristus yang adalah inkarnasi Allah turun ke dalam dunia, masuk dalam sejarah umat manusia. Yesus Kristus berperan sebagai Juruselamat manusia bahkan dikatakan satu-satunya jalan menuju ke Sorga (Yohanes 4:16). Tidak ada seorangpun, pemuka agama manapun, pendiri agama manapun yang dapat melakukan hal ini. Pribadi kedua dari Allah Trinitas inilah yang menjelma menjadi manusia bernama Yesus Kristus, sesuai dengan yang dikatakan kitab suci sebagai Juruselamat manusia. Yesus Kristus menggenapi semua pemberitaan nabi-nabi dalam keseluruhan kitab Perjanjian Lama, mulai dari kitab Taurat, kitab nabi besar dan nabi kecil, artinya sebagai wahyu terakhir atau pernyataan Allah yang final bagi keselamatan umat manusia. Dikatakan sebagai wahyu terakhir karena nubuatan kitab suci mengatakan demikian, dan dipertegas lagi oleh penulis kitab Ibrani bahwa pada zaman akhir, Allah berbicara kepada manusia lewat perantaraan Anak-Nya (Ibrani 1:2).Penelitian ini berusaha menjawab tentang sosok Yesus Kristus yang diyakini orang-orang percaya (Kristen) tentang iman mereka yang meyakini seutuhnya bahwa Yesus Kristus (Sang Logos) itu adalah wahyu terakhir Allah, yang mana sebelumnya di dalam kitab-kitab Perjanjian Lama, Allah telah berbicara kepada umat-Nya lewat perantaraan nabi-nabi-Nya kepada Israel. Dan di zaman akhir inilah, Allah berbicara lewat perantaraan Anak-Nya yang diutus ke dalam dunia untuk menggenapi seluruh kitab Perjanjian Lama. Maksud tujuan Yesus Kristus sebagai sebuah penggenapan dari karya Allah untuk keselamatan umat manusia dari kengerian kekal Di dalam sejarah keselamatan manusia, sejak manusia pertama yang diciptakan TUHAN jatuh ke dalam dosa, manusia seharusnya menerima upahnya yaitu mati kekal karena pelanggaran atau ketidaktaatan Adam dan Hawa saat itu (Kejadian 2:17). Tetapi inisiatif Allahlah yang merencanakan keselamatan bagi manusia hingga saat ini (Kejadian 3:21).  Penggenapan itu dengan mengutus Yesus Kristus yang adalah inkarnasi Allah turun ke dalam dunia, masuk dalam sejarah umat manusia. Yesus Kristus berperan sebagai Juruselamat manusia bahkan dikatakan satu-satunya jalan menuju ke Sorga (Yohanes 4:16). Tidak ada seorangpun, pemuka agama manapun, pendiri agama manapun yang dapat melakukan hal ini. Pribadi kedua dari Allah Trinitas inilah yang menjelma menjadi manusia bernama Yesus Kristus, sesuai dengan yang dikatakan kitab suci sebagai Juruselamat manusia. Yesus Kristus menggenapi semua pemberitaan nabi-nabi dalam keseluruhan kitab Perjanjian Lama, mulai dari kitab Taurat, kitab nabi besar dan nabi kecil, artinya sebagai wahyu terakhir atau pernyataan Allah yang final bagi keselamatan umat manusia. Dikatakan sebagai wahyu terakhir karena nubuatan kitab suci mengatakan demikian, dan dipertegas lagi oleh penulis kitab Ibrani bahwa pada zaman akhir, Allah berbicara kepada manusia lewat perantaraan Anak-Nya (Ibrani 1:2)

Association of Over-The-Counter Pharmaceutical Sales with Influenza-Like-Illnesses to Patient Volume in an Urgent Care Setting

We studied the association between OTC pharmaceutical sales and volume of patients with influenza-like-illnesses (ILI) at an urgent care center over one year. OTC pharmaceutical sales explain 36% of the variance in the patient volume, and each standard deviation increase is associated with 4.7 more patient visits to the urgent care center (p<0.0001). Cross-correlation function analysis demonstrated that OTC pharmaceutical sales are significantly associated with patient volume during non-flu season (p<0.0001), but only the sales of cough and cold (p<0.0001) and thermometer (p<0.0001) categories were significant during flu season with a lag of two and one days, respectively. Our study is the first study to demonstrate and measure the relationship between OTC pharmaceutical sales and urgent care center patient volume, and presents strong evidence that OTC sales predict urgent care center patient volume year round. © 2013 Liu et al

Kinetic Characterisation of a Single Chain Antibody against the Hormone Abscisic Acid: Comparison with Its Parental Monoclonal

A single-chain Fv fragment antibody (scFv) specific for the plant hormone abscisic acid (ABA) has been expressed in the bacterium Escherichia coli as a fusion protein. The kinetics of ABA binding have been measured using surface plasmon resonance spectrometry (BIAcore 2000) using surface and solution assays. Care was taken to calculate the concentration of active protein in each sample using initial rate measurements under conditions of partial mass transport limitation. The fusion product, parental monoclonal antibody and the free scFv all have low nanomolar affinity constants, but there is a lower dissociation rate constant for the parental monoclonal resulting in a three-fold greater affinity. Analogue specificity was tested and structure-activity binding preferences measured. The biologically-active (+)-ABA enantiomer is recognised with an affinity three orders of magnitude higher than the inactive (-)-ABA. Metabolites of ABA including phaseic acid, dihydrophaseic acid and deoxy-ABA have affinities over 100-fold lower than that for (+)-ABA. These properties of the scFv make it suitable as a sensor domain in bioreporters specific for the naturally occurring form of ABA

Histone deacetylase adaptation in single ventricle heart disease and a young animal model of right ventricular hypertrophy.

BackgroundHistone deacetylase (HDAC) inhibitors are promising therapeutics for various forms of cardiac diseases. The purpose of this study was to assess cardiac HDAC catalytic activity and expression in children with single ventricle (SV) heart disease of right ventricular morphology, as well as in a rodent model of right ventricular hypertrophy (RVH).MethodsHomogenates of right ventricle (RV) explants from non-failing controls and children born with a SV were assayed for HDAC catalytic activity and HDAC isoform expression. Postnatal 1-day-old rat pups were placed in hypoxic conditions, and echocardiographic analysis, gene expression, HDAC catalytic activity, and isoform expression studies of the RV were performed.ResultsClass I, IIa, and IIb HDAC catalytic activity and protein expression were elevated in the hearts of children born with a SV. Hypoxic neonatal rats demonstrated RVH, abnormal gene expression, elevated class I and class IIb HDAC catalytic activity, and protein expression in the RV compared with those in the control.ConclusionsThese data suggest that myocardial HDAC adaptations occur in the SV heart and could represent a novel therapeutic target. Although further characterization of the hypoxic neonatal rat is needed, this animal model may be suitable for preclinical investigations of pediatric RV disease and could serve as a useful model for future mechanistic studies

What Does It Take? California County Funding Requests for Recovery-Oriented Full Service Partnerships Under the Mental Health Services Act

The need to move mental health systems toward more recovery-oriented treatment modes is well established. Progress has been made to define needed changes but evidence is lacking about the resources required to implement them. The Mental Health Services Act (MHSA) in California was designed to implement more recovery-oriented treatment modes. We use data from county funding requests and annual updates to examine how counties budgeted for recovery-oriented programs targeted to different age groups under MHSA. Findings indicate that initial per-client budgeting for Full Services Partnerships under MHSA was maintained in future cycles and counties budgeted less per client for children. With this analysis, we begin to benchmark resource allocation for programs that are intended to be recovery-oriented, which should be evaluated against appropriate outcome measures in the future to determine the degree of recovery-orientation

Automated inter-rater reliability assessment and electronic data collection in a multi-center breast cancer study

<p>Abstract</p> <p>Background</p> <p>The choice between paper data collection methods and electronic data collection (EDC) methods has become a key question for clinical researchers. There remains a need to examine potential benefits, efficiencies, and innovations associated with an EDC system in a multi-center medical record review study.</p> <p>Methods</p> <p>A computer-based automated menu-driven system with 658 data fields was developed for a cohort study of women aged 65 years or older, diagnosed with invasive histologically confirmed primary breast cancer (N = 1859), at 6 Cancer Research Network sites. Medical record review with direct data entry into the EDC system was implemented. An inter-rater and intra-rater reliability (IRR) system was developed using a modified version of the EDC.</p> <p>Results</p> <p>Automation of EDC accelerated the flow of study information and resulted in an efficient data collection process. Data collection time was reduced by approximately four months compared to the project schedule and funded time available for manuscript preparation increased by 12 months. In addition, an innovative modified version of the EDC permitted an automated evaluation of inter-rater and intra-rater reliability across six data collection sites.</p> <p>Conclusion</p> <p>Automated EDC is a powerful tool for research efficiency and innovation, especially when multiple data collection sites are involved.</p

Extra-gastrointestinal manifestations of inflammatory bowel disease may be less common than previously reported

Extra-intestinal manifestations are well recognized in inflammatory bowel disease (IBD). To what extent the commonly recognized extra-intestinal manifestations seen in IBD patients are attributable to IBD is, however, not clear due to the limited number of controlled studies published

Evolutionary Analysis of Mitogenomes from Parasitic and Free-Living Flatworms

Copyright: © 2015 Solà et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The attached file is the published version of the article

The polygenic nature of telomere length and the anti-ageing properties of lithium

Telomere length is a promising biomarker for age-related disease and a potential anti-ageing drug target. Here, we study the genetic architecture of telomere length and the repositioning potential of lithium as an anti-ageing medication. LD score regression applied to the largest telomere length genome-wide association study to-date, revealed SNP-chip heritability estimates of 7.29%, with polygenic risk scoring capturing 4.4% of the variance in telomere length in an independent cohort (p = 6.17 × 10-5). Gene-enrichment analysis identified 13 genes associated with telomere length, with the most significant being the leucine rich repeat gene, LRRC34 (p = 3.69 × 10-18). In the context of lithium, we confirm that chronic use in a sample of 384 bipolar disorder patients is associated with longer telomeres (p = 0.03). As complementary evidence, we studied three orthologs of telomere length regulators in a Caenorhabditis elegans model of lithium-induced extended longevity and found all transcripts to be affected post-treatment (p  0.05). Consequently, this suggests that lithium may be catalysing the activity of endogenous mechanisms that promote telomere lengthening, whereby its efficacy eventually becomes limited by each individual's inherent telomere maintenance capabilities. Our work indicates a potential use of polygenic risk scoring for the prediction of adult telomere length and consequently lithium's anti-ageing efficacy

Truncated and Helix-Constrained Peptides with High Affinity and Specificity for the cFos Coiled-Coil of AP-1

Protein-based therapeutics feature large interacting surfaces. Protein folding endows structural stability to localised surface epitopes, imparting high affinity and target specificity upon interactions with binding partners. However, short synthetic peptides with sequences corresponding to such protein epitopes are unstructured in water and promiscuously bind to proteins with low affinity and specificity. Here we combine structural stability and target specificity of proteins, with low cost and rapid synthesis of small molecules, towards meeting the significant challenge of binding coiled coil proteins in transcriptional regulation. By iteratively truncating a Jun-based peptide from 37 to 22 residues, strategically incorporating i-->i+4 helix-inducing constraints, and positioning unnatural amino acids, we have produced short, water-stable, alpha-helical peptides that bind cFos. A three-dimensional NMR-derived structure for one peptide (24) confirmed a highly stable alpha-helix which was resistant to proteolytic degradation in serum. These short structured peptides are entropically pre-organized for binding with high affinity and specificity to cFos, a key component of the oncogenic transcriptional regulator Activator Protein-1 (AP-1). They competitively antagonized the cJun–cFos coiled-coil interaction. Truncating a Jun-based peptide from 37 to 22 residues decreased the binding enthalpy for cJun by ~9 kcal/mol, but this was compensated by increased conformational entropy (TDS ≤ 7.5 kcal/mol). This study demonstrates that rational design of short peptides constrained by alpha-helical cyclic pentapeptide modules is able to retain parental high helicity, as well as high affinity and specificity for cFos. These are important steps towards small antagonists of the cJun-cFos interaction that mediates gene transcription in cancer and inflammatory diseases