1,448 research outputs found
Spinor Helicity and Dual Conformal Symmetry in Ten Dimensions
The spinor helicity formalism in four dimensions has become a very useful
tool both for understanding the structure of amplitudes and also for practical
numerical computation of amplitudes. Recently, there has been some discussion
of an extension of this formalism to higher dimensions. We describe a
particular implementation of the spinor-helicity method in ten dimensions.
Using this tool, we study the tree-level S-matrix of ten dimensional super
Yang-Mills theory, and prove that the theory enjoys a dual conformal symmetry.
Implications for four-dimensional computations are discussed.Comment: 24 pages, 1 figure
OA031-04. Impairment of HIV-1-specific CD8+ T cell function by soluble epithelial adhesion molecules
Superconformal symmetry and two-loop amplitudes in planar N=4 super Yang-Mills
Scattering amplitudes in superconformal field theories do not enjoy this
symmetry, because the definition of asymptotic states involve a notion of
infinity. Concentrating on planar Yang-Mills, we consider a
generalization of scattering amplitudes which depends on twice as many
Grassmann variables. We conjecture that it restores at least half of the
superconformal symmetries, and all of the dual superconformal symmetries. The
object arises naturally as the dual of a null polygonal Wilson loop in an
superspace. We support the conjecture by using it to
obtain the total differential of all -point two-loop MHV amplitudes, and
showing that the result passes consistency checks. Potential all-loop
constraints are also discussed.Comment: 25 pages, 2 figures and 1 noteboo
Yangian symmetry of light-like Wilson loops
We show that a certain class of light-like Wilson loops exhibits a Yangian
symmetry at one loop, or equivalently, in an Abelian theory. The Wilson loops
we discuss are equivalent to one-loop MHV amplitudes in N=4 super Yang-Mills
theory in a certain kinematical regime. The fact that we find a Yangian
symmetry constraining their functional form can be thought of as the effect of
the original conformal symmetry associated to the scattering amplitudes in the
N=4 theory.Comment: 15 pages, 5 figure
A New Stalked Filter-Feeder from the Middle Cambrian Burgess Shale, British Columbia, Canada
Burgess Shale-type deposits provide invaluable insights into the early evolution of body plans and the ecological structure of Cambrian communities, but a number of species, continue to defy phylogenetic interpretations. Here we extend this list to include a new soft-bodied animal, Siphusauctum gregarium n. gen. and n. sp., from the Tulip Beds (Campsite Cliff Shale Member, Burgess Shale Formation) of Mount Stephen (Yoho National Park, British Columbia). With 1,133 specimens collected, S. gregarium is clearly the most abundant animal from this locality
Immunoseq: the identification of functionally relevant variants through targeted capture and sequencing of active regulatory regions in human immune cells
: The observation that the genetic variants identified in genome-wide association studies (GWAS) frequently lie in non-coding regions of the genome that contain cis-regulatory elements suggests that altered gene expression underlies the development of many complex traits. In order to efficiently make a comprehensive assessment of the impact of non-coding genetic variation in immune related diseases we emulated the whole-exome sequencing paradigm and developed a custom capture panel for the known DNase I hypersensitive site (DHS) in immune cells - "Immunoseq".
: We performed Immunoseq in 30 healthy individuals where we had existing transcriptome data from T cells. We identified a large number of novel non-coding variants in these samples. Relying on allele specific expression measurements, we also showed that our selected capture regions are enriched for functional variants that have an impact on differential allelic gene expression. The results from a replication set with 180 samples confirmed our observations.
: We show that Immunoseq is a powerful approach to detect novel rare variants in regulatory regions. We also demonstrate that these novel variants have a potential functional role in immune cells.This work was supported by grants from the Canadian Institute of Health Research (CIHR), the UK Medical Research Council (G1100125), the Swedish Research Council (DO283001) and Knut and Alice Wallenberg Foundation (KAW). We also acknowledge the use of subjects from the Cambridge BioResource and the support of the Cambridge NIHR Biomedical Research Centre. AM was supported by the Fond de Recherche Santé Québec Doctoral training award. TP and CL holds a Canada Research Chair
Massive amplitudes on the Coulomb branch of N=4 SYM
We initiate a systematic study of amplitudes with massive external particles
on the Coulomb-branch of N=4 super Yang Mills theory: 1) We propose that
(multi-)soft-scalar limits of massless amplitudes at the origin of moduli space
can be used to determine Coulomb-branch amplitudes to leading order in the
mass. This is demonstrated in numerous examples. 2) We find compact explicit
expressions for several towers of tree-level amplitudes, including scattering
of two massive W-bosons with any number of positive helicity gluons, valid for
all values of the mass. 3) We present the general structure of superamplitudes
on the Coulomb branch. For example, the n-point "MHV-band" superamplitude is
proportional to a Grassmann polynomial of mixed degree 4 to 12, which is
uniquely determined by supersymmetry. We find explicit tree-level
superamplitudes for this MHV band and for other simple sectors of the theory.
4) Dual conformal generators are constructed, and we explore the dual conformal
properties of the simplest massive amplitudes. Our compact expressions for
amplitudes and superamplitudes should be of both theoretical and
phenomenological interest; in particular the tree-level results carry over to
truncations of the theory with less supersymmetry.Comment: 29 pages, 1 figur
PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration
Background: Elucidating the complex cell dynamics (divisions, movement, morphological changes, etc.) underlying embryonic development and adult tissue regeneration requires an efficient means to track cells with high fidelity in space and time. To satisfy this criterion, we developed a transgenic zebrafish line, called PhOTO, that allows photoconvertible optical tracking of nuclear and membrane dynamics in vivo.
Methodology: PhOTO zebrafish ubiquitously express targeted blue fluorescent protein (FP) Cerulean and photoconvertible FP Dendra2 fusions, allowing for instantaneous, precise targeting and tracking of any number of cells using Dendra2 photoconversion while simultaneously monitoring global cell behavior and morphology. Expression persists through adulthood, making the PhOTO zebrafish an excellent tool for studying tissue regeneration: after tail fin amputation and photoconversion of a ~100µm stripe along the cut area, marked differences seen in how cells contribute to the new tissue give detailed insight into the dynamic process of regeneration. Photoconverted cells that contributed to the regenerate were separated into three distinct populations corresponding to the extent of cell division 7 days after amputation, and a subset of cells that divided the least were organized into an evenly spaced, linear orientation along the length of the newly regenerating fin.
Conclusions/Significance: PhOTO zebrafish have wide applicability for lineage tracing at the systems-level in the early embryo as well as in the adult, making them ideal candidate tools for future research in development, traumatic injury and regeneration, cancer progression, and stem cell behavior
Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos
<p>Abstract</p> <p>Background</p> <p>The use of genetically-encoded fluorescent proteins has revolutionized the fields of cell and developmental biology and in doing so redefined our understanding of the dynamic morphogenetic processes that shape the embryo. With the advent of more accessible and sophisticated imaging technologies as well as an abundance of fluorescent proteins with different spectral characteristics, the dynamic processes taking place <it>in situ </it>in living cells and tissues can now be probed. Photomodulatable fluorescent proteins are one of the emerging classes of genetically-encoded fluorescent proteins.</p> <p>Results</p> <p>We have compared PA-GFP, PS-CFP2, Kaede and KikGR four readily available and commonly used photomodulatable fluorescent proteins for use in ES cells and mice. Our results suggest that the green-to-red photoconvertible fluorescent protein, Kikume Green-Red (KikGR), is most suitable for cell labeling and lineage studies in ES cells and mice because it is developmentally neutral, bright and undergoes rapid and complete photoconversion. We have generated transgenic ES cell lines and strains of mice exhibiting robust widespread expression of KikGR. By efficient photoconversion of KikGR we labeled subpopulations of ES cells in culture, and groups of cells within <it>ex utero </it>cultured mouse embryos. Red fluorescent photoconverted cells and their progeny could be followed for extended periods of time.</p> <p>Conclusion</p> <p>Transgenic ES cells and mice exhibiting widespread readily detectable expression of KikGR are indistinguishable from their wild type counterparts and are amenable to efficient photoconversion. They represent novel tools for non-invasive selective labeling specific cell populations and live imaging cell dynamics and cell fate. Genetically-encoded photomodulatable proteins such as KikGR represent emergent attractive alternatives to commonly used vital dyes, tissue grafts and genetic methods for investigating dynamic behaviors of individual cells, collective cell dynamics and fate mapping applications.</p
Precursors to social and communication difficulties in infants at-risk for autism: gaze following and attentional engagement
Whilst joint attention (JA) impairments in autism have been widely studied, little is known about the early development of gaze following, a precursor to establishing JA. We employed eye-tracking to record gaze following longitudinally in infants with and without a family history of autism spectrum disorder (ASD) at 7 and 13 months. No group difference was found between at-risk and low-risk infants in gaze following behaviour at either age. However, despite following gaze successfully at 13 months, at-risk infants with later emerging socio-communication difficulties (both those with ASD and atypical development at 36 months of age) allocated less attention to the congruent object compared to typically developing at-risk siblings and low-risk controls. The findings suggest that the subtle emergence of difficulties in JA in infancy may be related to ASD and other atypical outcomes
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