Photometric Recovery of Crowded Stellar Fields Observed with HST/WFPC2 and the Effects of Confusion Noise on the Extragalactic Distance Scale

We explore the limits of photometric reductions of crowded stellar fields observed with the Wide Field and Planetary Camera 2 on board the Hubble Space Telescope. Two photometric procedures, based on the DoPHOT and DAOPHOT/ALLFRAME programs are tested, and the effects of crowding, complex sky background and cosmic-ray contamination are discussed using an extensive set of artificial star simulations. As a specific application of the results presented in this paper, we assess the magnitude of photometric biases on programs aimed at finding Cepheids and determining distances. We find that while the photometry in individual images can be biased too bright by up to 0.2 mag in the most crowded fields due to confusion noise, the effects on distance measurements based on Cepheid variables are insignificant, less than 0.02 mag (1% in distance) even in the most problematic cases. This result, which is at odds with claims recently surfaced in the literature, is due to the strict criteria applied in the selection of the variable stars, and the photometric cross checks made possible by the availability of multiple exposures in different filters which characterizes Cepheid observations.Comment: Accepted for publication in PASP. 41 pages, 18 figures, 8 tables. The figures included with this submission are very low quality bitmap postscript, please see http://www.astro.ucla.edu/~laura/pub.htm for the full size image

Structural and Compositional Changes in Aging Bone: Osteopenia in Lumbar Vertebrae of Wistar Female Rats

Changes in vertebral bone mineral content and density during aging were quantified in female Wistar rats. This study represents a longitudinal follow up utilizing single photon absorptiometry for the measurement of bone mineral content (BMC), quantitative computed tomography (OCT) for the measurement of bone mineral density (BMD), and image analysis histomorphometry for the measurement of trabecular bone volume (TBV) and bone cortical area (BCA). The above measurements were accompanied by biochemical assays of calcium concentrations in the respective bones. All aging animals experienced significant decreases in BMC, BMD, TBV, BCA and in the calcium content of their bones. The above features have been further emphasized through the use of scanning electron micrographs showing the age-related structural changes in a three-dimentional fashion. New, advanced technologies will enable the quantitation of 3-dimensional images that are currently obtained from the scanning electron micrograph; thus will provide new consideration as related to trabecular bone compactness (density). Energy dispersive x-ray spectroscopy indicated that the nature of crystals in aging bones does not differ markedly from that encountered in young specimens. Data are also provided with regard to the health of the animals, and it became apparent that aging rats undergo changes in their kidneys yet do not show any significant change in renal functional parameters as measured in both the serum and the urine. Hence, new noninvasive methodologies are currently available for longitudinal studies related to the skeleton in laboratory animals enabling reliable monitoring of age-related and hormonally induced changes in bones (spine and hip) of well defined experimental models

The Porcupine Survey: A Distributed Survey and WISE Followup

Spitzer post-cryogen observations to perform a moderate depth survey distributed around the sky are proposed. Field centers are chosen to be WISE brown dwarf candidates, which will typically be 160 µJy at 4.7 µm and randomly distributed around the sky. The Spitzer observations will give much higher sensitivity, higher angular resolution, and a time baseline to measure both proper motions and possibly parallaxes. The distance and velocity data obtained on the WISE brown dwarf candidates will greatly improve our knowledge of the mass and age distribution of brown dwarfs. The outer parts of the Spitzer fields surrounding the WISE positions will provide a deep survey in many narrow fields of view distributed around the sky, and the volume of this survey will contain many more distant brown dwarfs, and many extragalactic objects

The Spitzer Warm Mission Science Prospects

After exhaustion of its cryogen, the Spitzer Space telescope will still have a fully functioning two-channel mid-IR camera that will have sensitivities better than any other ground or space-based telescopes until the launch of JWST. This document provides a description of the expected capabilities of Spitzer during its warm mission phase, and provides brief descriptions of several possible very large science programs that could be conducted. This information is intended to serve as input to a wide ranging discussion of the warm mission science, leading up to the Warm Mission Workshop in June 2007

A Cepheid Distance to NGC 4603 in Centaurus

In an attempt to use Cepheid variables to determine the distance to the Centaurus cluster, we have obtained images of NGC 4603 with the Hubble Space Telescope on 9 epochs using WFPC2 and the F555W and F814W filters. This galaxy has been suggested to lie within the ``Cen30'' portion of the cluster and is the most distant object for which this method has been attempted. Previous distance estimates for Cen30 have varied significantly and some have presented disagreements with the peculiar velocity predicted from redshift surveys, motivating this investigation. Using our observations, we have found 61 candidate Cepheid variable stars; however, a significant fraction of these candidates are likely to be nonvariable stars whose magnitude measurement errors happen to fit a Cepheid light curve of significant amplitude for some choice of period and phase. Through a maximum likelihood technique, we determine that we have observed 43 +/- 7 real Cepheids and that NGC 4603 has a distance modulus of 32.61 +0.11/-0.10 (random, 1 sigma) +0.24/-0.25 (systematic, adding in quadrature), corresponding to a distance of 33.3 Mpc. This is consistent with a number of recent estimates of the distance to NGC 4603 or Cen30 and implies a small peculiar velocity consistent with predictions from the IRAS 1.2 Jy redshift survey if the galaxy lies in the foreground of the cluster.Comment: Accepted for publication in the Astrophysical Journal. 17 pages with 17 embedded figures and 3 tables using emulateapj.sty. Additional figures and images may be obtained from http://astro.berkeley.edu/~marc/n4603

Ongoing evolution of proposal reviews in the Spitzer warm mission

The Spitzer Space Telescope is executing the seventh year of extended warm mission science. The cryogenic mission operated from 2003 to 2009. The observing proposal review process has evolved from large, week-long, in-person meetings during the cryogenic mission to the introduction of panel telecon reviews in the warm mission. Further compression of the schedule and budget for the proposal solicitation and selection process led to additional changes in 2014. Large proposals are still reviewed at an in-person meeting but smaller proposals are no longer discussed by a topical science panel. This hybrid process, involving an in-person committee for the larger proposals and strictly external reviewers for the smaller proposals, has been successfully implemented through two observing cycles. While people like the idea of not having to travel to a review it is still the consensus opinion, in our discussions with the community, that the in-person review panel discussions provide the most satisfying result. We continue to use in-person reviews for awarding greater than 90% of the observing time

New distances of unresolved dwarf elliptical galaxies in the vicinity of the Local Group

We present Surface Brightness Fluctuation distances of nine early-type dwarf galaxies and the S0 galaxy NGC 4150 in the Local Volume based on deep B- and R-band CCD images obtained with the 2.56 metre Nordic Optical Telescope. Typically, six stellar fields at various galactocentric distances have been chosen for each galaxy as appropriately free of foreground stars and other contaminants, and Fourier analysed to determine the distances, which are found to lie in the range of 3 to 16 Mpc. The SBF method is thus demonstrated to efficiently measure distances from the ground with mid-aperture telescopes for galaxies for which only the tip of the red giant branch method in combination with the Hubble Space Telescope has been available until now. We obtained the following distance moduli: 28.11 +/- 0.15 mag (or 4.2 +/- 0.3 Mpc) for UGC 1703, 27.61 +/- 0.17 mag (or 3.3 +/- 0.3 Mpc) for KDG 61, 29.00 +/- 0.27 mag (or 6.3 +/- 0.8 Mpc) for UGCA 200, 27.74 +/- 0.18 mag (or 3.5 +/- 0.3 Mpc) for UGC 5442, 30.22 +/- 0.17 mag (or 11.1 +/- 0.9 Mpc) for UGC 5944, 30.79 +/- 0.11 mag (or 14.4 +/- 0.7 Mpc) for NGC 4150, 31.02 +/- 0.25 mag (or 16.0 +/- 1.9 Mpc) for BTS 128, 29.27 +/- 0.16 mag (or 7.1 +/- 0.6 Mpc) for UGC 7639, 30.19 +/- 0.23 mag (or 10.9 +/- 1.2 Mpc) for UGC 8799 with an alternative distance of 30.61 +/- 0.26 mag (or 13.2 +/- 1.7 Mpc), and 29.60 +/- 0.20 mag (or 8.3 +/- 0.8 Mpc) for UGC 8882.Comment: 14 pages, 13 figures (figures 1, 2 and 10 are of reduced quality), accepted for publication in A&

Pea3 Transcription Factors and Wnt1-Induced Mouse Mammary Neoplasia

The role of the PEA3 subfamily of Ets transcription factors in breast neoplasia is controversial. Although overexpression of PEA3 (E1AF/ETV4), and of the related factors ERM (ETV5) and ER81 (ETV1), have been observed in human and mouse breast tumors, PEA3 factors have also been ascribed a tumor suppressor function. Here, we utilized the MMTV/Wnt1 mouse strain to further interrogate the role of PEA3 transcription factors in mammary tumorigenesis based on our previous observation that Pea3 is highly expressed in MMTV/Wnt1 mammary tumors. Pea3 expression in mouse mammary tissues was visualized using a Pea3NLSlacZ reporter strain. In normal mammary glands, Pea3 expression is predominantly confined to myoepithelial cells. Wnt1 transgene expression induced marked amplification of this cell compartment in nontumorous mammary glands, accompanied by an apparent increase in Pea3 expression. The pattern of Pea3 expression in MMTV/Wnt1 mammary glands recapitulated the cellular profile of activated β-catenin/TCF signaling, which was visualized using both β-catenin immunohistochemistry and the β-catenin/TCF-responsive reporter Axin2NLSlacZ. To test the requirement for PEA3 factors in Wnt1-induced tumorigenesis, we employed a mammary-targeted dominant negative PEA3 transgene, ΔNPEA3En. Expression of ΔNPEA3En delayed early-onset tumor formation in MMTV/Wnt1 virgin females (P = 0.03), suggesting a requirement for PEA3 factor function for Wnt1-driven tumor formation. Consistent with this observation, expression of the ΔNPEA3En transgene was profoundly reduced in mammary tumors compared to nontumorous mammary glands from bigenic MMTV/Wnt1, MMTV/ΔNPEA3En mice (P = 0.01). Our data provide the first description of Wnt1-mediated expansion of the Pea3-expressing myoepithelial compartment in nontumorous mammary glands. Consistent with this observation, mammary myoepithelium was selectively responsive to Wnt1. Together these data suggest the MMTV/Wnt1 strain as a potential model of basal breast cancer. Furthermore, this study provides evidence for a protumorigenic role of PEA3 factors in breast neoplasia, and supports targeting the PEA3 transcription factor family in breast cancer

A Database of Cepheid Distance Moduli and TRGB, GCLF, PNLF and SBF Data Useful for Distance Determinations

We present a compilation of Cepheid distance moduli and data for four secondary distance indicators that employ stars in the old stellar populations: the planetary nebula luminosity function (PNLF), the globular cluster luminosity function (GCLF), the tip of the red giant branch (TRGB), and the surface brightness fluctuation (SBF) method. The database includes all data published as of July 15, 1999. The main strength of this compilation resides in all data being on a consistent and homogeneous system: all Cepheid distances are derived using the same calibration of the period-luminosity relation, the treatment of errors is consistent for all indicators, measurements which are not considered reliable are excluded. As such, the database is ideal for inter-comparing any of the distance indicators considered, or for deriving a Cepheid calibration to any secondary distance indicator. Specifically, the database includes: 1) Cepheid distances, extinctions and metallicities; 2) apparent magnitudes of the PNLF cutoff; 3) apparent magnitudes and colors of the turnover of the GCLF (both in the V- and B-bands); 4) apparent magnitudes of the TRGB (in the I-band) and V-I colors at and 0.5 magnitudes fainter than the TRGB; 5) apparent surface brightness fluctuation magnitudes I, K', K_short, and using the F814W filter with the HST/WFPC2. In addition, for every galaxy in the database we give reddening estimates from DIRBE/IRAS as well as HI maps, J2000 coordinates, Hubble and T-type morphological classification, apparent total magnitude in B, and systemic velocity. (Abridged)Comment: Accepted for publication in the Astrophysical Journal Supplement Series. Because of space limitations, the figures included are low resolution bitmap images. Original figures can be found at http://www.astro.ucla.edu/~laura/pub.ht

EZH2 or HDAC1 Inhibition Reverses Multiple Myeloma-Induced Epigenetic Suppression of Osteoblast Differentiation

In multiple myeloma, osteolytic lesions rarely heal because of persistent suppressed osteoblast differentiation resulting in a high fracture risk. Herein, chromatin immunoprecipitation analyses reveal that multiple myeloma cells induce repressive epigenetic histone changes at the Runx2 locus that prevent osteoblast differentiation. The most pronounced multiple myeloma-induced changes were at the Runx2-P1 promoter, converting it from a poised bivalent state to a repressed state. Previously, it was observed that multiple myeloma induces the transcription repressor GFI1 in osteoblast precursors, which correlates with decreased Runx2 expression, thus prompting detailed characterization of the multiple myeloma and TNFα-dependent GFI1 response element within the Runx2-P1 promoter. Further analyses reveal that multiple myeloma-induced GFI1 binding to Runx2 in osteoblast precursors and recruitment of the histone modifiers HDAC1, LSD1, and EZH2 is required to establish and maintain Runx2 repression in osteogenic conditions. These GFI1-mediated repressive chromatin changes persist even after removal of multiple myeloma. Ectopic GFI1 is sufficient to bind to Runx2, recruit HDAC1 and EZH2, increase H3K27me3 on the gene, and prevent osteogenic induction of endogenous Runx2 expression. Gfi1 knockdown in MC4 cells blocked multiple myeloma-induced recruitment of HDAC1 and EZH2 to Runx2, acquisition of repressive chromatin architecture, and suppression of osteoblast differentiation. Importantly, inhibition of EZH2 or HDAC1 activity in pre-osteoblasts after multiple myeloma exposure in vitro or in osteoblast precursors from patients with multiple myeloma reversed the repressive chromatin architecture at Runx2 and rescued osteoblast differentiation.Implications: This study suggests that therapeutically targeting EZH2 or HDAC1 activity may reverse the profound multiple myeloma-induced osteoblast suppression and allow repair of the lytic lesions