MicroRNA-612 regulates the proliferation and epithelial-to-mesenchymal transition of human colon cancer cells via G protein-coupled receptor 132 (GPR132)

Purpose: To investigate the effect of microRNA-612 (miR-612) on human colon cancer cells, and the mechanism involved. Methods: Expressions of miR-612 and GPR132 were determined by quantitative real-time polymerase chain reaction (qRT-PCR)el , while cell viability was evaluated using cell counting kit-8 (CCK8) and colony formation assays. Dual luciferase assay was used to determine the interaction between miR-612 and GPR132, while cell migration and invasion were measured by Transwell assay. Results: The expression levels of miR-612 in colon cancer tissues and cell lines were significantly down-regulated (p < 0.05). Overexpression of miR-612 in colon cancer cells led to significant inhibition of their proliferation and colony formation. Transwell assays revealed that miR-612 overexpression markedly stopped the migration, invasion and epithelial-to-mesenchymal transition. Conclusion: These results indicate that miR-612 exerts anti-cancer effect by suppressing the expression of GPR132 at the translational level. The in vitro tumor suppressive activity of miR-612 against colon cancer reveals its potential for the management of colon cancer

Subthalamic nucleus dynamics track microlesion effect in Parkinson’s disease

Parkinson’s Disease (PD) is characterized by the temporary alleviation of motor symptoms following electrode implantation (or nucleus destruction), known as the microlesion effect (MLE). Electrophysiological studies have explored different PD stages, but understanding electrophysiological characteristics during the MLE period remains unclear. The objective was to examine the characteristics of local field potential (LFP) signals in the subthalamic nucleus (STN) during the hyperacute period following implantation (within 2 days) and 1 month post-implantation. 15 patients diagnosed with PD were enrolled in this observational study, with seven simultaneous recordings of bilateral STN-LFP signals using wireless sensing technology from an implantable pulse generator. Recordings were made in both on and off medication states over 1 month after implantation. We used a method to parameterize the neuronal power spectrum to separate periodic oscillatory and aperiodic components effectively. Our results showed that beta power exhibited a significant increase in the off medication state 1 month after implantation, compared to the postoperative hyperacute period. Notably, this elevation was effectively attenuated by levodopa administration. Furthermore, both the exponents and offsets displayed a decrease at 1 month postoperatively when compared to the hyperacute postoperative period. Remarkably, levodopa medication exerted a modulatory effect on these aperiodic parameters, restoring them back to levels observed during the hyperacute period. Our findings suggest that both periodic and aperiodic components partially capture distinct electrophysiological characteristics during the MLE. It is crucial to adequately evaluate such discrepancies when exploring the mechanisms of MLE and optimizing adaptive stimulus protocols

A Late Cretaceous amber biota from central Myanmar

International audienceInsect faunas are extremely rare near the latest Cretaceous with a 24-million-year gap spanning from the early Campanian to the early Eocene. Here, we report a unique amber biota from the Upper Cretaceous (uppermost Campanian ~72.1 Ma) of Tilin, central Myan-mar. The chemical composition of Tilin amber suggests a tree source among conifers, indicating that gymnosperms were still abundant in the latest Campanian equatorial forests. Eight orders and 12 families of insects have been found in Tilin amber so far, making it the latest known diverse insect assemblage in the Mesozoic. The presence of ants of the extant sub-families Dolichoderinae and Ponerinae supports that tropical forests were the cradle for the diversification of crown-group ants, and suggests that the turnover from stem groups to crown groups had already begun at ~72.1 Ma. Tilin amber biota fills a critical insect faunal gap and provides a rare insight into the latest Campanian forest ecosystem

Mechanical Properties of Nano-SiO2 Reinforced Geopolymer Concrete under the Coupling Effect of a Wet–Thermal and Chloride Salt Environment

In this study, the mechanical behaviors of nano-SiO2 reinforced geopolymer concrete (NS-GPC) under the coupling effect of a wet–thermal and chloride salt environment were investigated through a series of basic experiments, and a simulation on the coupling effect of a wet–thermal and chloride salt environment and SEM test were also included. During the experiments for the coupling effect of the wet–thermal and chloride salt environment, an environment simulation test chamber was utilized to simulate the wet–thermal and chloride salt environment, in which the parameters of relative humidity, temperature, mass fraction of NaCl solution and action time were set as 100%, 45 °C, 5% and 60 d, respectively. The content of nano-SiO2 (NS) particles added in geopolymer concrete (GPC) were 0, 0.5%, 1.0%, 1.5% and 2.0%. The result indicated that the mechanical properties of NS reinforced GPC decreased under the coupling effect of the wet–thermal and chloride salt environment compared to the control group in the natural environment. When the NS content was 1.5%, the cube and splitting tensile strength, elastic modulus and impact toughness of GPC under the coupling environment of wet–thermal and chloride salt were decreased by 9.7%, 9.8%, 19.2% and 44.4%, respectively, relative to that of the GPC under the natural environment. The addition of NS improved the mechanical properties of GPC under the coupling effect of the wet–thermal and chloride salt environment. Compared to the control group without NS, the maximum increment in cube compressive strength, splitting tensile strength and elastic modulus of NS–GPC under the coupling effect of the wet–thermal and chloride salt environment due to the incorporation of NS reached 25.8%, 9.6% and 17.2%, respectively. Specifically, 1.5% content of NS increased the impact toughness, impact numbers of initial crack and the ultimate failure of GPC by 122.3%, 109% and 109.5%, respectively

Construction of Hierarchical MoSe2 Hollow Structures and Its Effecton Electrochemical Energy Storage and Conversion

Metal selenides have attracted increased attentionas promising electrode materials for electrochemical energy storageand conversion systems including metal-ion batteries and watersplitting. However, their practical application is greatly hindered bycollapse of the microstructure, thus leading to performance fading.Tuning the structure at nanoscale of these materials is an effectivestrategy to address the issue. Herein, we craft MoSe2withhierarchical hollow structures via a facile bubble-assistedsolvothermal method. The temperature-related variations of thehollow interiors are studied, which can be presented as solid, yolk−shell, and hollow spheres, respectively. Under the simultaneousaction of the distinctive hollow structures and interconnectionsamong the nanosheets, more intimate contacts between MoSe2and electrolyte can be achieved, thereby leading to superior electrochemical properties. Consequently, the MoSe2hollownanospheres prepared under optimum conditions exhibit optimal electrochemical activities, which hold an initial specificcapacity of 1287 mA h g−1and maintain great capacity even after 100 cycles as anode for Li-ion battery. Moreover, the Tafelslope of 58.9 mV dec−1for hydrogen evolution reaction is also attained

Revelation and Projection of Historic and Future Precipitation Characteristics in the Haihe River Basin, China

Precipitation, as one of the main components of the hydrological cycle, is known to be significantly impacted by global climate change. In recent years, the frequency of extreme precipitation has increased, resulting in greater destructiveness. Atmospheric circulation has a significant impact on extreme precipitation in a region. This study aims to investigate the prospective changes in extreme precipitation and their relationship with large-scale atmospheric circulation in the Haihe River Basin. The Haihe River Basin is located in the North China Plain. Mountains and plains can be found in both the eastern and western parts of the study region. The summer seasons experience the most precipitation. The monthly and extreme precipitation (based on daily precipitation) results from the Coupled Model Intercomparison Project Phase 6 (CMIP6) models were evaluated using observed precipitation data, which was utilized as a reference. The CMIP6 models were used to assess future changes in the characteristics of extreme precipitation in the study region. The relationship between extreme precipitation and large-scale atmospheric circulation was also analyzed using historical observation data. Remote sensing results regarding land cover and soil erosion were used to analyze the risks of extreme precipitation and their influences in the study region. According to the results, their multi-model ensembles (MME) and BCC-CSM2-MR models, respectively, outperformed all other CMIP6 models in simulating monthly and extreme (based on daily precipitation) precipitation over the study region. Extreme precipitation demonstrated a rising degree of contribution and future risk under numerous scenarios. The degrees of contribution of R95p and R99p are anticipated to increase in the future. BCC-CSM2-MR predicted that Rx1day and Rx5day would decline in the future. Generally, extreme precipitation increased to a greater degree under SSP585 than under SSP245. Both the El Niño–Southern Oscillation and the Pacific Decadal Oscillation displayed substantial resonance with the extreme precipitation from 1962 to 1980 and around 1995, respectively. This study not only improves our understanding of the occurrence of extreme precipitation, but it also serves as a reference for flood control and waterlogging prevention in the Haihe River Basin

Additional file 1: Table S1. of Identification and characterization of a maize-associated mastrevirus in China by deep sequencing small RNA populations

Primers used for PCR amplification. Table S2. Contigs found to have identity to Maize streak Reunion virus in a BLASTn search of the NCBI data base. Table S3. Sequences of selected members belonging to the Geminiviridae used in the phylogenetic analysis. (DOC 75 kb

Genesis of the Dongpuzi Gold Deposit in the Liaodong Peninsula, NE China: Constraints from Geology, Fluid Inclusion, and C–H–O–S–Pb Isotopes

The Dongpuzi deposit is an epithermal gold deposit located in the southern margin of the Shaozihe volcanic fault basin in the Liaodong Peninsula. On the basis of fluid inclusion and C–H–O–S–Pb isotope data, a metallogenic model is established for the Dongpuzi deposit. The mineralization at the Dongpuzi deposit has experienced quartz–pyrite (I), quartz–sulfide (II), and quartz–calcite (III) stages. The quartz from ore stage II has liquid-dominated aqueous inclusions, which have homogenization temperatures ranging from 113 to 162 °C and salinities varying from 3.2 to 9.6 wt% NaCl equiv. The quartz from the quartz–calcite stage has decreasing homogenization temperatures (106~143 °C) and salinities (2.7~6.9 wt% NaCl equiv.). The fluid inclusion data indicate that the gold ores were precipitated from low-temperature and low-salinity solutions, with an obvious decrease in temperature and salinity from ore stages II to III. The calculated δ18Owater values for the quartz of ore stage II range from −14.71‰ to −13.31‰, and the corresponding δDwater values range from −103.3‰ to −96.1‰, indicating that the ore-forming fluids could be of a meteoric origin. The calcite from ore stage III has δ13CV-PDB values of −4.5‰ to −4.2‰ and δ18OV-SMOW values of +7.0‰ to +7.4‰, indicating a mantle source for the carbon. The pyrite yielded δ34S values of +4.1‰ to +6.6‰ and Pb isotopes consistent with those of the host trachyte porphyry and volcanic rocks of the Xiaoling Formation, which suggests that the S and Pb in gold ores were dominantly derived from the host trachyte porphyry and volcanic rocks of the Xiaoling Formation, with some combination of Paleoproterozoic metamorphic rocks of the Gaixian Formation. These results, together with the ore geology, indicate that the Dongpuzi deposit is a typical low-sulfidation epithermal gold deposit with important ore-forming materials input from the host trachyte porphyry, volcanic rocks of the Xiaoling Formation, and Paleoproterozoic metamorphic rocks of the Gaixian Formation. The Dongpuzi deposit was formed under an extensional setting related to the Early Cretaceous lithospheric extension and thinning of the eastern North China Craton