Muscle and reflex changes with varying joint angle in hemiparetic stroke

<p>Abstract</p> <p>Background</p> <p>Despite intensive investigation, the origins of the neuromuscular abnormalities associated with spasticity are not well understood. In particular, the mechanical properties induced by stretch reflex activity have been especially difficult to study because of a lack of accurate tools separating reflex torque from torque generated by musculo-tendinous structures. The present study addresses this deficit by characterizing the contribution of neural and muscular components to the abnormally high stiffness of the spastic joint.</p> <p>Methods</p> <p>Using system identification techniques, we characterized the neuromuscular abnormalities associated with spasticity of ankle muscles in chronic hemiparetic stroke survivors. In particular, we systematically tracked changes in muscle mechanical properties and in stretch reflex activity during changes in ankle joint angle. Modulation of mechanical properties was assessed by applying perturbations at different initial angles, over the entire range of motion (ROM). Experiments were performed on both paretic and non-paretic sides of stroke survivors, and in healthy controls.</p> <p>Results</p> <p>Both reflex and intrinsic muscle stiffnesses were significantly greater in the spastic/paretic ankle than on the non-paretic side, and these changes were strongly position dependent. The major reflex contributions were observed over the central portion of the angular range, while the intrinsic contributions were most pronounced with the ankle in the dorsiflexed position.</p> <p>Conclusion</p> <p>In spastic ankle muscles, the abnormalities in intrinsic and reflex components of joint torque varied systematically with changing position over the full angular range of motion, indicating that clinical perceptions of increased tone may have quite different origins depending upon the angle where the tests are initiated.</p> <p>Furthermore, reflex stiffness was considerably larger in the non-paretic limb of stroke patients than in healthy control subjects, suggesting that the non-paretic limb may not be a suitable control for studying neuromuscular properties of the ankle joint.</p> <p>Our findings will help elucidate the origins of the neuromuscular abnormalities associated with stroke-induced spasticity.</p

Decreased Circulating Endothelial Progenitor Cell Levels and Function in Patients with Nonalcoholic Fatty Liver Disease

OBJECTIVES: Nonalcoholic fatty liver disease (NAFLD) is associated with advanced atherosclerosis and a higher risk of cardiovascular disease. Increasing evidence suggests that injured endothelial monolayer is regenerated by circulating bone marrow derived-endothelial progenitor cells (EPCs), and levels of circulating EPCs reflect vascular repair capacity. However, the relation between NAFLD and EPC remains unclear. Here, we tested the hypothesis that patients with nonalcoholic fatty liver disease (NAFLD) might have decreased endothelial progenitor cell (EPC) levels and attenuated EPC function. METHODS AND RESULTS: A total of 312 consecutive patients undergoing elective coronary angiography because of suspected coronary artery disease were screened and received examinations of abdominal ultrasonography between July 2009 and November 2010. Finally, 34 patients with an ultrasonographic diagnosis of NAFLD, and 68 age- and sex-matched controls without NAFLD were enrolled. Flow cytometry with quantification of EPC markers (defined as CD34(+), CD34(+)KDR(+), and CD34(+)KDR(+)CD133(+)) in peripheral blood samples was used to assess circulating EPC numbers. The adhesive function, and migration, and tube formation capacities of EPCs were also determined in NAFLD patients and controls. Patients with NAFLD had a significantly higher incidence of metabolic syndrome, previous myocardial infarction, hyperuricemia, and higher waist circumference, body mass index, fasting glucose and triglyceride levels. In addition, patients with NAFLD had significantly decreased circulating EPC levels (all P<0.05), attenuated EPC functions, and enhanced systemic inflammation compared to controls. Multivariate logistic regression analysis showed that circulating EPC level (CD34(+)KDR(+) [cells/10(5) events]) was an independent reverse predictor of NAFLD (Odds ratio: 0.78; 95% confidence interval: 0.69-0.89, P<0.001). CONCLUSIONS: NAFLD patients have decreased circulating EPC numbers and functions than those without NAFLD, which may be one of the mechanisms to explain atherosclerotic disease progression and enhanced cardiovascular risk in patients with NAFLD

Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells

As populations age across the world, osteoporosis and osteoporosis-related fractures are becoming the most prevalent degenerative bone diseases. More than 75 million patients suffer from osteoporosis in the US, the EU and Japan. Furthermore, it is anticipated that the number of patients affected by osteoporosis will increase by a third by 2050. Although conventional therapies including bisphosphonates, calcitonin and oestrogen-like drugs can be used to treat degenerative diseases, they are often associated with serious side effects including the development of oesophageal cancer, ocular inflammation, severe musculoskeletal pain, and osteonecrosis of the jaw. The use of autologous mesenchymal stromal cells/mesenchymal stem cells (MSCs) is a possible alternative therapeutic approach to tackle osteoporosis while overcoming the limitations of traditional treatment options. However, osteoporosis can cause a decrease in the numbers of MSCs, induce their senescence, and lower their osteogenic differentiation potential. Three-dimensional (3D) cell culture is an emerging technology that allows a more physiological expansion and differentiation of stem cells compared to cultivation on conventional flat systems. This review will discuss current understanding of the effects of different 3D cell culture systems on proliferation, viability, osteogenic differentiation, as well as on the immunomodulatory and anti-inflammatory potential of MSCs

Investigation of Yasuj Landfill Leachate and its Impact on lawer Water Resource Quality (No.6 Tang‌konareh well)

Background & aim: One of the potential environmental hazards threatening the water resources is the influence of leachate from municipal wastes landfill.Since the Tang-e-‌Konareh drinking water wells are located downstream of landfill waste, the aim of this study was to evaluate the effect of landfill leachate on water quality parameters of Tang-e-‌Konareh well No.6water resource. Methods: In the present analytical study, twenty-six quality parameters of water of Tang-e‌-Konaneh well number 6 in tight and wet seasons were selected and analyzed by standard and Hach standard methods. Optional parameters included turbidity, biochemical oxygen demand, chemical oxygen demand, electrical conductivity, total dissolved solids, alkalinity, total hardness, calcium, magnesium, sulfate, phosphate, nitrate, chloride, fluoride, sodium, potassium, total coliform, fecal coliform, Cu, Fe, Mn, Pb, Cd, Cr and Ni, respectively. The results were compared with the standards of the Iran water industry. Results: In all of the samples from Tang-e-‌Konareh well No.6, the measured parameters were less than desirable, except lead-ion concentration and total hardness. Probably due to the fact that the concentration of these two parameters is that the groundwater flows through the bed of limestone formations and minerals leaded. In contrast, in dry seasons, most of the parameters increased due to increase of the concentration of the chemical components. Conclusion: According to the results, threats by water pollution and landfill waste wells downstream in Yasuj resources are predictable in the long term indirectly, therefore, necessary measures should be considered

Analysis of the thermomechanical response of granular materials by infrared thermography

International audienceGranular materials are defined as a collection of solid particles whose macroscopic mechanical behavior is governed by the interaction forces between the particles. Full-field experimental data on these materials remain few compared to numerical results, even though a wide literature deals with optical imaging (combined with digital image correlation) and photoelasticimetry (to measure shear stresses in particles made of birefringent materials). We applied infrared thermography to analyze two-dimensional granular media composed of cylinders and subjected to confined compression. We analyzed the calorific signature of the contact forces, especially by revealing mechanical dissipation in the interparticle friction zones. Moreover, two constitutive materials featuring entropic and isentropic elasticity were employed to compare distinct types of thermoelastic couplings. Couplings and mechanical dissipation were separately identified at two observation scales. The perspective of this work is the experimental analysis of soft granular media

Identification of interparticle contacts in granular media Using mechanoluminescent materials.

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