134 research outputs found
An Adversarial Approach for Explainable AI in Intrusion Detection Systems
Despite the growing popularity of modern machine learning techniques (e.g.
Deep Neural Networks) in cyber-security applications, most of these models are
perceived as a black-box for the user. Adversarial machine learning offers an
approach to increase our understanding of these models. In this paper we
present an approach to generate explanations for incorrect classifications made
by data-driven Intrusion Detection Systems (IDSs). An adversarial approach is
used to find the minimum modifications (of the input features) required to
correctly classify a given set of misclassified samples. The magnitude of such
modifications is used to visualize the most relevant features that explain the
reason for the misclassification. The presented methodology generated
satisfactory explanations that describe the reasoning behind the
mis-classifications, with descriptions that match expert knowledge. The
advantages of the presented methodology are: 1) applicable to any classifier
with defined gradients. 2) does not require any modification of the classifier
model. 3) can be extended to perform further diagnosis (e.g. vulnerability
assessment) and gain further understanding of the system. Experimental
evaluation was conducted on the NSL-KDD99 benchmark dataset using Linear and
Multilayer perceptron classifiers. The results are shown using intuitive
visualizations in order to improve the interpretability of the results
Multiplicity and transverse momentum fluctuations in inelastic proton-proton interactions at the CERN Super Proton Synchrotron
Measurements of multiplicity and transverse momentum fluctuations of charged
particles were performed in inelastic p+p interactions at 20, 31, 40, 80 and
158 GeV/c beam momentum. Results for the scaled variance of the multiplicity
distribution and for three strongly intensive measures of multiplicity and
transverse momentum fluctuations \$\Delta[P_{T},N]\$, \$\Sigma[P_{T},N]\$ and
\$\Phi_{p_T}\$ are presented. For the first time the results on fluctuations
are fully corrected for experimental biases. The results on multiplicity and
transverse momentum fluctuations significantly deviate from expectations for
the independent particle production. They also depend on charges of selected
hadrons. The string-resonance Monte Carlo models EPOS and UrQMD do not describe
the data. The scaled variance of multiplicity fluctuations is significantly
higher in inelastic p+p interactions than in central Pb+Pb collisions measured
by NA49 at the same energy per nucleon. This is in qualitative disagreement
with the predictions of the Wounded Nucleon Model. Within the statistical
framework the enhanced multiplicity fluctuations in inelastic p+p interactions
can be interpreted as due to event-by-event fluctuations of the fireball energy
and/or volume.Comment: 18 pages, 12 figure
Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51
Cancer stem cells (CSCs) are tumor subpopulations driving disease development, progression, relapse and therapy resistance, and their targeting ensures tumor eradication. CSCs display heterogeneous replication stress (RS), but the functionality/relevance of the RS response (RSR) centered on the ATR-CHK1 axis is debated. Here, we show that the RSR is efficient in primary CSCs from colorectal cancer (CRC-SCs), and describe unique roles for PARP1 and MRE11/RAD51. First, we demonstrated that PARP1 is upregulated in CRC-SCs resistant to several replication poisons and RSR inhibitors (RSRi). In these cells, PARP1 modulates replication fork speed resulting in low constitutive RS. Second, we showed that MRE11 and RAD51 cooperate in the genoprotection and mitosis execution of PARP1-upregulated CRC-SCs. These roles represent therapeutic vulnerabilities for CSCs. Indeed, PARP1i sensitized CRC-SCs to ATRi/CHK1i, inducing replication catastrophe, and prevented the development of resistance to CHK1i. Also, MRE11i + RAD51i selectively killed PARP1-upregulated CRC-SCs via mitotic catastrophe. These results provide the rationale for biomarker-driven clinical trials in CRC using distinct RSRi combinations
NA61/SHINE facility at the CERN SPS: beams and detector system
NA61/SHINE (SPS Heavy Ion and Neutrino Experiment) is a multi-purpose
experimental facility to study hadron production in hadron-proton,
hadron-nucleus and nucleus-nucleus collisions at the CERN Super Proton
Synchrotron. It recorded the first physics data with hadron beams in 2009 and
with ion beams (secondary 7Be beams) in 2011.
NA61/SHINE has greatly profited from the long development of the CERN proton
and ion sources and the accelerator chain as well as the H2 beamline of the
CERN North Area. The latter has recently been modified to also serve as a
fragment separator as needed to produce the Be beams for NA61/SHINE. Numerous
components of the NA61/SHINE set-up were inherited from its predecessors, in
particular, the last one, the NA49 experiment. Important new detectors and
upgrades of the legacy equipment were introduced by the NA61/SHINE
Collaboration.
This paper describes the state of the NA61/SHINE facility - the beams and the
detector system - before the CERN Long Shutdown I, which started in March 2013
Nucleus Basalis of Meynert Stimulation for Dementia: Theoretical and Technical Considerations
Deep brain stimulation (DBS) of nucleus basalis of Meynert (NBM) is currently being evaluated as a potential therapy to improve memory and overall cognitive function in dementia. Although, the animal literature has demonstrated robust improvement in cognitive functions, phase 1 trial results in humans have not been as clear-cut. We hypothesize that this may reflect differences in electrode location within the NBM, type and timing of stimulation, and the lack of a biomarker for determining the stimulation’s effectiveness in real time. In this article, we propose a methodology to address these issues in an effort to effectively interface with this powerful cognitive nucleus for the treatment of dementia. Specifically, we propose the use of diffusion tensor imaging to identify the nucleus and its tracts, quantitative electroencephalography (QEEG) to identify the physiologic response to stimulation during programming, and investigation of stimulation parameters that incorporate the phase locking and cross frequency coupling of gamma and slower oscillations characteristic of the NBM’s innate physiology. We propose that modulating the baseline gamma burst stimulation frequency, specifically with a slower rhythm such as theta or delta will pose more effective coupling between NBM and different cortical regions involved in many learning processes
Consensus guidelines for the definition, detection and interpretation of immunogenic cell death.
Cells succumbing to stress via regulated cell death (RCD) can initiate an adaptive immune response associated with immunological memory, provided they display sufficient antigenicity and adjuvanticity. Moreover, multiple intracellular and microenvironmental features determine the propensity of RCD to drive adaptive immunity. Here, we provide an updated operational definition of immunogenic cell death (ICD), discuss the key factors that dictate the ability of dying cells to drive an adaptive immune response, summarize experimental assays that are currently available for the assessment of ICD in vitro and in vivo, and formulate guidelines for their interpretation
Enhanced repair of DNA interstrand crosslinking in ovarian cancer cells from patients following treatment with platinum-based chemotherapy
Despite high tumour response rates to platinum-based chemotherapy in ovarian cancer survival is poor due to the emergence of drug resistance. Mechanistic studies in clinical material have been hampered by the unavailability of sensitive methods to detect the critical drug-induced effects in individual cells. A modification of the single cell gel electrophoresis (comet) assay allows the sensitive detection of DNA interstrand crosslinking in both tumour and normal cells derived directly from clinical material. Tumour cells isolated from 50 ovarian cancer patients were treated ex vivo with 100 μM cisplatin for 1 h and crosslink formation and repair (unhooking) measured. No significant difference in the peak level of crosslinking in tumour cells was observed between patients who were either newly diagnosed or previously treated with platinum-based therapy, or between tumour and mesothelial cells from an individual patient. This indicates no difference in cellular mechanisms such as drug transport or detoxification. In contrast, the percentage repair (unhooking) of DNA interstrand crosslinks was much greater in the group of treated patients. At 24 h in the 36 newly diagnosed patient tumour samples, only one gave >50% repair and 23 gave <10% repair; however, 19 out of 22 treated patient samples gave >10% repair and 14 showed >50% repair. The estimated median difference (newly diagnosed minus treated) was −52 (95% CI −67 to −28), and the P-value from a Mann–Whitney test was <0.001. In eight patients, it was possible to obtain tumour samples prior to any chemotherapy, and also on relapse or at interval debulking surgery following platinum-based chemotherapy. In these patients, the mean % repair prior to therapy was 2.85 rising to 71.23 following treatment. These data demonstrate increased repair of DNA interstrand crosslinks in ovarian tumour cells following platinum therapy which may contribute to clinical acquired resistance
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