The GBT Diffuse Ionized Gas Survey (GDIGS): Discrete Sources

The Green Bank Telescope (GBT) Diffuse Ionized Gas Survey (GDIGS) traces ionized gas in the Galactic midplane by observing radio recombination line (RRL) emission from 4–8 GHz. The nominal survey zone is 32.3◦ \u3e ℓ \u3e −5◦, | b | \u3c 0.5◦. Here, we analyze GDIGS Hnα ionized gas emission toward discrete sources. Using GDIGS data, we identify the velocity of 35 H II regions that have multiple detected RRL velocity components. We identify and characterize RRL emission from 88 H II regions that previously lacked measured ionized gas velocities. We also identify and characterize RRL emission from eight locations that appear to be previously-unidentified H II regions and 30 locations of RRL emission that do not appear to be H II regions based on their lack of mid-infrared emission. This latter group may be a compact component of the Galactic Diffuse Ionized Gas (DIG). There are an additional 10 discrete sources that have anomalously high RRL velocities for their locations in the Galactic plane. We compare these objects’ RRL data to 13CO, H I and mid-infrared data, and find that these sources do not have the expected 24 µm emission characteristic of H II regions. Based on this comparison we do not think these objects are H II regions, but we are unable to classify them as a known type of object

The Vaccinia Virus (VACV) B1 and Cellular VRK2 Kinases Promote VACV Replication Factory Formation through Phosphorylation-Dependent Inhibition of VACV B12

Comparative examination of viral and host protein homologs reveals novel mechanisms governing downstream signaling effectors of both cellular and vi- ral origin. The vaccinia virus B1 protein kinase is involved in promoting multiple facets of the virus life cycle and is a homolog of three conserved cellular enzymes called vaccinia virus-related kinases (VRKs). Recent evidence indicates that B1 and VRK2 mediate a com- mon pathway that is largely uncharacterized but appears independent of previous VRK substrates. Interestingly, separate studies described a novel role for B1 in inhibiting vac- cinia virus protein B12, which otherwise impedes an early event in the viral lifecycle. Herein, we characterize the B1/VRK2 signaling axis to better understand their shared functions. First, we demonstrate that vaccinia virus uniquely requires VRK2 for viral repli- cation in the absence of B1, unlike other DNA viruses. Employing loss-of-function analy- sis, we demonstrate that vaccinia virus’s dependence on VRK2 is only observed in the presence of B12, suggesting that B1 and VRK2 share a pathway controlling B12. More- over, we substantiate a B1/VRK2/B12 signaling axis by examining coprecipitation of B12 by B1 and VRK2. Employing execution point analysis, we reveal that virus replication proceeds normally through early protein translation and uncoating but stalls at replica- tion factory formation in the presence of B12 activity. Finally, structure/function analyses of B1 and VRK2 demonstrate that enzymatic activity is essential for B1 or VRK2 to inhibit B12. Together, these data provide novel insights into B1/VRK signaling coregulation and support a model in which these enzymes modulate B12 in a phosphorylation-depen- dent manner

The Vaccinia Virus (VACV) B1 and Cellular VRK2 Kinases Promote VACV Replication Factory Formation through Phosphorylation-Dependent Inhibition of VACV B12

Comparative examination of viral and host protein homologs reveals novel mechanisms governing downstream signaling effectors of both cellular and viral origin. The vaccinia virus B1 protein kinase is involved in promoting multiple facets of the virus life cycle and is a homolog of three conserved cellular enzymes called vaccinia virus-related kinases (VRKs). Recent evidence indicates that B1 and VRK2 mediate a common pathway that is largely uncharacterized but appears independent of previous VRK substrates. Interestingly, separate studies described a novel role for B1 in inhibiting vaccinia virus protein B12, which otherwise impedes an early event in the viral lifecycle. Herein, we characterize the B1/VRK2 signaling axis to better understand their shared functions. First, we demonstrate that vaccinia virus uniquely requires VRK2 for viral replication in the absence of B1, unlike other DNA viruses. Employing loss-of-function analysis, we demonstrate that vaccinia virus’s dependence on VRK2 is only observed in the presence of B12, suggesting that B1 and VRK2 share a pathway controlling B12. Moreover, we substantiate a B1/VRK2/B12 signaling axis by examining coprecipitation of B12 by B1 and VRK2. Employing execution point analysis, we reveal that virus replication proceeds normally through early protein translation and uncoating but stalls at replication factory formation in the presence of B12 activity. Finally, structure/function analyses of B1 and VRK2 demonstrate that enzymatic activity is essential for B1 or VRK2 to inhibit B12. Together, these data provide novel insights into B1/VRK signaling coregulation and support a model in which these enzymes modulate B12 in a phosphorylation-dependent manner

Combinatorial roles for zebrafish retinoic acid receptors in the hindbrain, limbs and pharyngeal arches

AbstractRetinoic acid (RA) signaling regulates multiple aspects of vertebrate embryonic development and tissue patterning, in part through the local availability of nuclear hormone receptors called retinoic acid receptors (RARs) and retinoid receptors (RXRs). RAR/RXR heterodimers transduce the RA signal, and loss-of-function studies in mice have demonstrated requirements for distinct receptor combinations at different stages of embryogenesis. However, the tissue-specific functions of each receptor and their individual contributions to RA signaling in vivo are only partially understood. Here we use morpholino oligonucleotides to deplete the four known zebrafish RARs (raraa, rarab, rarga, and rargb). We show that while all four are required for anterior–posterior patterning of rhombomeres in the hindbrain, there are unique requirements for rarga in the cranial mesoderm for hindbrain patterning, and rarab in lateral plate mesoderm for specification of the pectoral fins. In addition, the alpha subclass (raraa, rarab) is RA inducible, and of these only raraa expression is RA-dependent, suggesting that these receptors establish a region of particularly high RA signaling through positive-feedback. These studies reveal novel tissue-specific roles for RARs in controlling the competence and sensitivity of cells to respond to RA

Generation of cloned transgenic pigs rich in omega-3 fatty acids

Meat products are generally low in omega-3 (n-3) fatty acids, which are beneficial to human health. We describe the generation of cloned pigs that express a humanized Caenorhabditis elegans gene, fat-1, encoding an n-3 fatty acid desaturase. The hfat-1 transgenic pigs produce high levels of n-3 fatty acids from n-6 analogs, and their tissues have a significantly reduced ratio of n-6/n-3 fatty acids (P < 0.001). © 2006 Nature Publishing Group

The GBT Diffuse Ionized Gas Survey (GDIGS): Survey Overview and First Data Release

The Green Bank Telescope (GBT) Diffuse Ionized Gas Survey (GDIGS) traces ionized gas in the Galactic midplane by measuring $4-8$GHz radio recombination line (RRL) emission. The nominal survey zone is $32.3^{\circ}> l >-5^{\circ}$, $|b|<0.5^{\circ}$, but coverage extends above and below the plane in select fields, and additionally includes the areas around W47 ($l \simeq 37.5^{\circ}$) and W49 ($l \simeq 43^{\circ}$). GDIGS simultaneously observes 22 Hn$\alpha$ (15 usable), 25 Hn$\beta$ (18 usable), and 8 Hn$\gamma$ RRLs (all usable), as well as multiple molecular line transitions (including of H$_2^{13}$CO, H$_2$CO, and CH$_3$OH). Here, we describe the GDIGS survey parameters and characterize the RRL data, focusing primarily on the Hn$\alpha$ data. We produce sensitive data cubes by averaging the usable RRLs, after first smoothing to a common spectral resolution of 0.5km/s and a spatial resolution of 2.65' for Hn$\alpha$, 2.62' for Hn$\beta$, and 2.09' for Hn$\gamma$. The average spectral noise per spaxel in the \hna\ data cubes is $\sim\!10$mK ($\sim\!5$mJy/beam). This sensitivity allows GDIGS to detect RRLs from plasma throughout the inner Galaxy. The GDIGS Hn$\alpha$ data are sensitive to emission measures $EM \gtrsim 1100$cm$^{-6}$pc, which corresponds to a mean electron density $\langle n_e \rangle \gtrsim 30$cm$^{-3}$ for a 1pc path length or $\langle n_e \rangle \gtrsim 1$cm$^{-3}$ for a 1kpc path length.Comment: Accepted for publication by ApJS. Data may be downloaded here: http://astro.phys.wvu.edu/gdigs

Complexity of the self-schema and responses to disconfirming feedback

This study focused on complexity of the self-schema as one factor that influences people's responses to social feedback that challenges their established view of self. Complexity refers to the number of independent attributes included in the schema. A card-sorting task (Zajonc, 1960) was used to identify the high- and low-complexity groups. Subjects were given bogus feedback relevant to the targeted domain of self-knowledge, and changes in self-descriptiveness ratings and response latency times were monitored. Results suggest that high-complexity subjects were able to attend to and encode the disconfirming feedback, while low-complexity subjects responded by rejecting the feedback and reasserting positive aspects of the self. The implications of these findings for clarifying the process of self-schema updating, revision, and change are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44337/1/10608_2006_Article_BF02357222.pd

Perceiving Mixed Valence Emotions Reduces Intergroup Dehumanization

To deny others’ humanity is one of the most heinous forms of intergroup prejudice. Given evidence that perceiving various forms of complexity in outgroup members reduces intergroup prejudice, we investigated across three experiments whether the novel dimension of emotional complexity, or outgroup members’ joint experience of mixed-valence emotions, would also reduce their dehumanization. Experiment 1 found that perceiving fictitious aliens’ experience of the same primary emotions (e.g., sadness) presented in mixed vs. non-mixed valence pairs led to reduced prejudice via attenuated dehumanization, i.e. attribution of uniquely human emotions. Experiment 2 confirmed these results, using an unfamiliar real-world group as an outgroup target. Experiment 3 used a familiar outgroup and found generally similar effects, reducing social distance through reduced dehumanization. These processes suggest that an alternate route to reduced dehumanizing of outgroups might involve presenting mixed valence emotions

Vocal Fundamental and Formant Frequencies Affect Perceptions of Speaker Cooperativeness

In recent years, the perception of social traits in faces and voices has received much attention. Facial and vocal masculinity are linked to perceptions of trustworthiness; however, while feminine faces are generally considered to be trustworthy, vocal trustworthiness is associated with masculinized vocal features. Vocal traits such as pitch and formants have previously been associated with perceived social traits such as trustworthiness and dominance, but the link between these measurements and perceptions of cooperativeness have yet to be examined. In Experiment 1, cooperativeness ratings of male and female voices were examined against four vocal measurements: fundamental frequency (F0), pitch variation (F0&minus;SD), formant dispersion (Df), and formant position (Pf). Feminine pitch traits (F0 and F0&minus;SD) and masculine formant traits (Df and Pf) were associated with higher cooperativeness ratings. In Experiment 2, manipulated voices with feminized F0 were found to be more cooperative than voices with masculinized F0, among both male and female speakers, confirming our results from Experiment 1. Feminine pitch qualities may indicate an individual who is friendly and non-threatening, while masculine formant qualities may reflect an individual that is socially dominant or prestigious, and the perception of these associated traits may influence the perceived cooperativeness of the speakers

The association between depressive symptoms and executive control impairments in response to emotional and non-emotional information

Depression has been linked with impaired executive control and specific impairments in inhibition of negative material. To date, only a few studies have examined the relationship between depressive symptoms and executive functions in response to emotional information. Using a new paradigm, the Affective Shift Task (AST), the present study examined whether depressive symptoms in general, and rumination specifically, are related to impairments in inhibition and set shifting in response to emotional and non-emotional material. The main finding was that depressive symptoms in general were not related to inhibition. Set-shifting impairments were only observed in moderate to severely depressed individuals. Interestingly, rumination was related to inhibition impairments, specifically when processing negative information, as well as impaired set shifting as reflected in a larger shift cost. These results are discussed in relation to cognitive views on vulnerability for depression