Large-scale multielectrode recording and stimulation of neural activity

Large circuits of neurons are employed by the brain to encode and process information. How this encoding and processing is carried out is one of the central questions in neuroscience. Since individual neurons communicate with each other through electrical signals (action potentials), the recording of neural activity with arrays of extracellular electrodes is uniquely suited for the investigation of this question. Such recordings provide the combination of the best spatial (individual neurons) and temporal (individual action-potentials) resolutions compared to other large-scale imaging methods. Electrical stimulation of neural activity in turn has two very important applications: it enhances our understanding of neural circuits by allowing active interactions with them, and it is a basis for a large variety of neural prosthetic devices. Until recently, the state-of-the-art in neural activity recording systems consisted of several dozen electrodes with inter-electrode spacing ranging from tens to hundreds of microns. Using silicon microstrip detector expertise acquired in the field of high-energy physics, we created a unique neural activity readout and stimulation framework that consists of high-density electrode arrays, multi-channel custom-designed integrated circuits, a data acquisition system, and data-processing software. Using this framework we developed a number of neural readout and stimulation systems: (1) a 512-electrode system for recording the simultaneous activity of as many as hundreds of neurons, (2) a 61-electrode system for electrical stimulation and readout of neural activity in retinas and brain-tissue slices, and (3) a system with telemetry capabilities for recording neural activity in the intact brain of awake, naturally behaving animals. We will report on these systems, their various applications to the field of neurobiology, and novel scientific results obtained with some of them. We will also outline future directions

The story of supernova 'Refsdal' told by MUSE

We present MUSE observations in the core of the HFF galaxy cluster MACS J1149.5+2223, where the first magnified and spatially-resolved multiple images of SN 'Refsdal' at redshift 1.489 were detected. Thanks to a DDT program with the VLT and the extraordinary efficiency of MUSE, we measure 117 secure redshifts with just 4.8 hours of total integration time on a single target pointing. We spectroscopically confirm 68 galaxy cluster members, with redshift values ranging from 0.5272 to 0.5660, and 18 multiple images belonging to 7 background, lensed sources distributed in redshifts between 1.240 and 3.703. Starting from the combination of our catalog with those obtained from extensive spectroscopic and photometric campaigns using the HST, we select a sample of 300 (164 spectroscopic and 136 photometric) cluster members, within approximately 500 kpc from the BCG, and a set of 88 reliable multiple images associated to 10 different background source galaxies and 18 distinct knots in the spiral galaxy hosting SN 'Refsdal'. We exploit this valuable information to build 6 detailed strong lensing models, the best of which reproduces the observed positions of the multiple images with a rms offset of only 0.26". We use these models to quantify the statistical and systematic errors on the predicted values of magnification and time delay of the next emerging image of SN 'Refsdal'. We find that its peak luminosity should should occur between March and June 2016, and should be approximately 20% fainter than the dimmest (S4) of the previously detected images but above the detection limit of the planned HST/WFC3 follow-up. We present our two-dimensional reconstruction of the cluster mass density distribution and of the SN 'Refsdal' host galaxy surface brightness distribution. We outline the roadmap towards even better strong lensing models with a synergetic MUSE and HST effort.Comment: 21 pages, 9 figures, 6 tables; accepted for publication in the Astrophysical Journal - extra information on data analysis added, all model predictions and results unchange

A highly-ionized region surrounding SN Refsdal revealed by MUSE

Supernova (SN) Refsdal is the first multiply-imaged, highly-magnified, and spatially-resolved SN ever observed. The SN exploded in a highly-magnified spiral galaxy at z=1.49 behind the Frontier Fields Cluster MACS1149, and provides a unique opportunity to study the environment of SNe at high z. We exploit the time delay between multiple images to determine the properties of the SN and its environment, before, during, and after the SN exploded. We use the integral-field spectrograph MUSE on the VLT to simultaneously target all observed and model-predicted positions of SN Refsdal. We find MgII emission at all positions of SN Refsdal, accompanied by weak FeII* emission at two positions. The measured ratios of [OII] to MgII emission of 10-20 indicate a high degree of ionization with low metallicity. Because the same high degree of ionization is found in all images, and our spatial resolution is too coarse to resolve the region of influence of SN Refsdal, we conclude that this high degree of ionization has been produced by previous SNe or a young and hot stellar population. We find no variability of the [OII] line over a period of 57 days. This suggests that there is no variation in the [OII] luminosity of the SN over this period, or that the SN has a small contribution to the integrated [OII] emission over the scale resolved by our observations.Comment: 5 pages, 4 figures, accepted for publication in A&

CLASH-VLT: Testing the Nature of Gravity with Galaxy Cluster Mass Profiles

We use high-precision kinematic and lensing measurements of the total mass profile of the dynamically relaxed galaxy cluster MACS J1206.2-0847 at $z=0.44$ to estimate the value of the ratio $\eta=\Psi/\Phi$ between the two scalar potentials in the linear perturbed Friedmann-Lemaitre-Robertson-Walker metric.[...] Complementary kinematic and lensing mass profiles were derived from exhaustive analyses using the data from the Cluster Lensing And Supernova survey with Hubble (CLASH) and the spectroscopic follow-up with the Very Large Telescope (CLASH-VLT). Whereas the kinematic mass profile tracks only the time-time part of the perturbed metric (i.e. only $\Phi$), the lensing mass profile reflects the contribution of both time-time and space-space components (i.e. the sum $\Phi+\Psi$). We thus express $\eta$ as a function of the mass profiles and perform our analysis over the radial range $0.5\,Mpc\le r\le r_{200}=1.96\,Mpc$. Using a spherical Navarro-Frenk-White mass profile, which well fits the data, we obtain \eta(r_{200})=1.01\,_{-0.28}^{+0.31} at the 68\% C.L. We discuss the effect of assuming different functional forms for mass profiles and of the orbit anisotropy in the kinematic reconstruction. Interpreting this result within the well-studied $f(R)$ modified gravity model, the constraint on $\eta$ translates into an upper bound to the interaction length (inverse of the scalaron mass) smaller than 2 Mpc. This tight constraint on the $f(R)$ interaction range is however substantially relaxed when systematic uncertainties in the analysis are considered. Our analysis highlights the potential of this method to detect deviations from general relativity, while calling for the need of further high-quality data on the total mass distribution of clusters and improved control on systematic effects.Comment: 18 pages, 3 figures, submitted to JCA

Microscopic mechanism for mechanical polishing of diamond (110) surfaces

Mechanically induced degradation of diamond, as occurs during polishing, is studied using total--energy pseudopotential calculations. The strong asymmetry in the rate of polishing between different directions on the diamond (110) surface is explained in terms of an atomistic mechanism for nano--groove formation. The post--polishing surface morphology and the nature of the polishing residue predicted by this mechanism are consistent with experimental evidence.Comment: 4 pages, 5 figure

Ionising the Intergalactic Medium by Star Clusters: The first empirical evidence

We present a VLT/X-Shooter spectroscopy of the Lyman continuum (LyC) emitting galaxy 'Ion2' at z=3.2121 and compare it to that of the recently discovered strongly lensed LyC-emitter at z=2.37, known as the 'Sunburst' arc. Three main results emerge from the X-Shooter spectrum: (a) the Lya has three distinct peaks with the central one at the systemic redshift, indicating a ionised tunnel through which both Lya and LyC radiation escape; (b) the large O32 oxygen index ([OIII]4959-5007 / [OII]3727-3729) of 9.18(-1.32/+1.82) is compatible to those measured in local (z~0.4) LyC leakers; (c) there are narrow nebular high-ionisation metal lines with \sigma_v < 20 km/s, which confirms the presence of young hot, massive stars. The HeII1640 appears broad, consistent with a young stellar component including Wolf-Rayet stars. Similarly, the Sunburst LyC-emitter shows a triple-peaked Lya profile and from VLT/MUSE spectroscopy the presence of spectral features arising from young hot and massive stars. The strong lensing magnification, (\mu > 20), suggests that this exceptional object is a gravitationally-bound star cluster observed at a cosmological distance, with a stellar mass M <~ 10^7 Msun and an effective radius smaller than 20 pc. Intriguingly, sources like Sunburst but without lensing magnification might appear as Ion2-like galaxies, in which unresolved massive star clusters dominate the ultraviolet emission. This work supports the idea that dense young star clusters can contribute to the ionisation of the IGM through holes created by stellar feedback.Comment: 13 pages, 9 figures and 1 table, MNRAS accepted. Some typos fixe

Recovery of gastric function in patients affected by chronic atrophic gastritis using L-cysteine (Acetium®): one year survey in comparison with a control group

Background and aim: Chronic Atrophic Gastritis (CAG) is a precancerous condition for gastric cancer (GC) as single risk factor, being a consequence of a previous Helicobacter pylori (Hp) infection or based on autoimmune mechanisms. Achlorhydria plays an important role towards the formation of a class I carcinogen, acetaldehyde, after food intake. L-cysteine has been claimed to be able to bind in a covalent way acetaldehyde when administered at meals. Methods: In this study we enrolled two CAG groups of patients, one treated whit 300 mg/daily of L-cysteine for one year, the other one untreated. We assessed gastric function lasting the one-year follow-up by using noninvasive surrogates, i.e. Pepsinogen I (PGI) and gastrin 17 (G17). Results: In the group of 77 CAG on therapy we found a statistically significant increase in PGI values and a decrease in G17 levels, in comparison with unchanged values in control group. Conclusions: L-cysteine seems able to provide a recovery in gastric function when administered in CAG patients and could be proposed as a possible therapy in such patients. (www.actabiomedica.it)

The influence of the Lande gg-factor in the classical general relativistic description of atomic and subatomic systems

We study the electromagnetic and gravitational fields of the proton and electron in terms of the Einstenian gravity via the introduction of an arbitrary Lande $g$-factor in the Kerr-Newman solution. We show that at length scales of the order of the reduced Compton wavelength, corrections from different values of the $g$-factor are not negligible and discuss the presence of general relativistic effects in highly ionized heavy atoms. On the other hand, since at the Compton-wavelength scale the gravitational field becomes spin dominated rather than mass dominated, we also point out the necessity of including angular momentum as a source of corrections to Newtonian gravity in the quantum description of gravity at this scale.Comment: 11 pages, 2 figure

Paving the way for the JWST: witnessing globular cluster formation at z>3

We report on five compact, extremely young (<10Myr) and blue (\beta_UV<-2.5, F_\lambda =\lambda^\beta) objects observed with VLT/MUSE at redshift 3.1169, 3.235, in addition to three objects at z=6.145. These sources are magnified by the Hubble Frontier Field galaxy clusters MACS~J0416 and AS1063. Their de-lensed half light radii (Re) are between 16 to 140pc, the stellar masses are ~1-20 X 10^6 Msun, the magnitudes are m_uv=28.8 - 31.4 (-17<Muv<-15) and specific star formation rates can be as large as ~800Gyr^-1. Multiple images of these systems are widely separated in the sky (up to 50'') and individually magnified by factors 3-40. Remarkably, the inferred physical properties of two objects are similar to those expected in some globular cluster formation scenarios, representing the best candidate proto-globular clusters (proto-GC) discovered so far. Rest-frame optical high dispersion spectroscopy of one of them at z=3.1169 yields a velocity dispersion \sigma_v~20km/s, implying a dynamical mass dominated by the stellar mass. Another object at z=6.145, with de-lensed Muv ~ -15.3 (m_uv ~ 31.4), shows a stellar mass and a star-formation rate surface density consistent with the values expected from popular GC formation scenarios. An additional star-forming region at z=6.145, with de-lensed m_uv ~ 32, a stellar mass of 0.5 X 10^6 Msun and a star formation rate of 0.06 Msun/yr is also identified. These objects currently represent the faintest spectroscopically confirmed star-forming systems at z>3, elusive even in the deepest blank fields. We discuss how proto-GCs might contribute to the ionization budget of the universe and augment Lya visibility during reionization. This work underlines the crucial role of JWST in characterizing the rest-frame optical and near-infrared properties of such low-luminosity high-z objects.Comment: 19 pages, 9 figures, 2 tables. MNRAS, version accepted by the refere

Direct Lyman continuum and Lyman-alpha escape observed at redshift 4

We report on the serendipitous discovery of a z=4.0, M1500=-22.20 star-forming galaxy (Ion3) showing copious Lyman continuum (LyC) leakage (~60% escaping), a remarkable multiple peaked Lya emission, and significant Lya radiation directly emerging at the resonance frequency. This is the highest redshift confirmed LyC emitter in which the ionising and Lya radiation possibly share a common ionised cavity (with N_HI<10^17.2 cm^-2). Ion3 is spatially resolved, it shows clear stellar winds signatures like the P-Cygni NV1240 profile, and has blue ultraviolet continuum (\beta = -2.5 +/- 0.25, F_\lambda~ \lambda^\beta) with weak low-ionisation interstellar metal lines. Deep VLT/HAWKI Ks and Spitzer/IRAC 3.6um and 4.5um imaging show a clear photometric signature of the Halpha line with equivalent width of 1000A rest-frame emerging over a flat continuum (Ks-4.5um ~ 0). From the SED fitting we derive a stellar mass of 1.5x10^9 Msun, SFR of 140 Msun/yr and age of ~10 Myr, with a low dust extinction, E(B-V)< 0.1, placing the source in the starburst region of the SFR-M^* plane. Ion3 shows similar properties of another LyC emitter previously discovered (z=3.21, Ion2, Vanzella et al. 2016). Ion3 (and Ion2) represents ideal high-redshift reference cases to guide the search for reionising sources at z>6.5 with JWST.Comment: Accepted for publication in MNRAS. 5 pages, 4 figures, 1 tabl