Effect of daily movement of dairy cattle to fresh grass in morning or afternoon on intake, grazing behaviour, rumen fermentation and milk production

Twenty Holstein cows were split into two equal groups to test the effect of daily move to a previously ungrazed strip after morning milking (MA) or afternoon milking (AA) on herbage intake, grazing behaviour, rumen characteristics and milk production using a randomized block design with three periods of 14 days each. Milking took place at 06.00 and 16.00 h. The chemical composition of grass was similar between treatments, but an interaction between treatment and time of sampling was found in all variables except acid detergent lignin (ADL). The most pronounced differences existed in sugar content. Grass sugar content was greatest following afternoon milking. However, the difference in sugar content in grass was much larger in MA (158 v 114 g/kg dry matter (DM) at 16.00 and 06.00 h, respectively) than in AA (147 v 129 g/kg DM at 16.00 and 06.00 h, respectively). Neutral detergent fibre (NDF) was significantly higher at 06.00 h than at 16.00 h (469 v 425 g/kg DM) in AA, but was equal between morning and afternoon in MA (453 g/kg DM). Herbage intake, determined using the n-alkane technique, did not differ between treatments. Grazing behaviour observed using IGER graze recorders were similar between treatments, except for ruminating time, bite rate and the number of ruminations and boli per period of the day. However, interactions between treatment and time in grazing behaviour variables were found. Grazing time was longer and number of bites was greater following allocation to a new plot (after milking in the morning in MA or milking in the afternoon in AA) when compared to allocation to the same plot after the subsequent milking per treatment (after milking in the afternoon or morning in MA and AA, respectively). In comparison to AA, grazing time in MA was more evenly distributed during the day but lower during the night. The combined effects of differences in grazing behaviour and chemical composition of the grass between treatments in different periods of the day probably caused higher intake of sugars in AA, resulting in a significantly higher non-glucogenic to glucogenic volatile fatty acid ratio (NGR) in the rumen in AA than MA. Milk fat content was lower in MA than AA, but milk production and milk protein and lactose content did not differ. In conclusion, time of allocation to a fresh plot altered the distribution of grazing behaviour variables over the day, and affected NGR and milk fat content, but herbage intake and milk production were not change

SOME MEGASPORES FROM SOUTH AFRICA AND AUSTRALIA

Dr. Plumstead has sent a number of samples from South Africa to us for maceration of the spores from them and for an attempt to determine the horizons from the megaspores only. Other details were not given. In addition to these samples we had some from Australia, and it became very interesting to compare the spores from S. Africa with those from Australia or other Gondwana deposits in India or Brasil

Measuring the impact of observations on the predictability of the Kuroshio Extension in a shallow-water model

In this paper sequential importance sampling is used to assess the impact of observations on a ensemble prediction for the decadal path transitions of the Kuroshio Extension (KE). This particle filtering approach gives access to the probability density of the state vector, which allows us to determine the predictive power — an entropy based measure — of the ensemble prediction. The proposed set-up makes use of an ensemble that, at each time, samples the climatological probability distribution. Then, in a post-processing step, the impact of different sets of observations is measured by the increase in predictive power of the ensemble over the climatological signal during one-year. The method is applied in an identical-twin experiment for the Kuroshio Extension using a reduced-gravity shallow water model. We investigate the impact of assimilating velocity observations from different locations during the elongated and the contracted meandering state of the KE. Optimal observations location correspond to regions with strong potential vorticity gradients. For the elongated state the optimal location is in the first meander of the KE. During the contracted state of the KE it is located south of Japan, where the Kuroshio separates from the coast

Start-up inertia as an origin for heterogeneous flow

For quite some time non-monotonic flow curve was thought to be a requirement for shear banded flows in complex fluids. Thus, in simple yield stress fluids shear banding was considered to be absent. Recent spatially resolved rheological experiments have found simple yield stress fluids to exhibit shear banded flow profiles. One proposed mechanism for the initiation of such transient shear banding process has been a small stress heterogeneity rising from the experimental device geometry. Here, using Computational Fluid Dynamics methods, we show that transient shear banding can be initialized even under homogeneous stress conditions by the fluid start-up inertia, and that such mechanism indeed is present in realistic experimental conditions

A review of mathematical functions for the analysis of growth in poultry

Poultry industries face various decisions in the production cycle that affect the profitability of an operation. Predictions of growth when the birds are ready for sale are important factors that contribute to the economy of poultry operations. Mathematical functions called ‘growth functions’ have been used to relate body weight (W) to age or cumulative feed intake. These can also be used as response functions to predict daily energy and protein dietary requirements for maintenance and growth (France et al., 1989). When describing growth versus age in poultry, a fixed point of inflexion can be a limitation with equations such as the Gompertz and logistic. Inflexion points vary depending on age, sex, breed and type of animal, so equations such as the Richards and López are generally recommended. For describing retention rate against daily intake, which generally does not exhibit an inflexion point, the monomolecular would appear the function of choice

Improving Dental Experiences by Using Virtual Reality Distraction: A Simulation Study

Dental anxiety creates significant problems for both patients and the dental profession. Some distraction interventions are already used by healthcare professionals to help patients cope with unpleasant procedures. The present study is novel because it a) builds on evidence that natural scenery is beneficial for patients, and b) uses a Virtual Reality (VR) representation of nature to distract participants. Extending previous work that has investigated pain and anxiety during treatment, c) we also consider the longer term effects in terms of more positive memories of the treatment, building on a cognitive theory of memory (Elaborated Intrusions). Participants (n = 69) took part in a simulated dental experience and were randomly assigned to one of three VR conditions (active vs. passive vs. control). In addition, participants were distinguished into high and low dentally anxious according to a median split resulting in a 362 between-subjects design. VR distraction in a simulated dental context affected memories a week later. The VR distraction had effects not only on concurrent experiences, such as perceived control, but longitudinally upon the vividness of memories after the dental experience had ended. Participants with higher dental anxiety (for whom the dental procedures were presumably more aversive) showed a greater reduction in memory vividness than lower dental-anxiety participants. This study thus suggests that VR distractions can be considered as a relevant intervention for cycles of care in which people’s previous experiences affect their behaviour for future events

Accelerated X-ray Structure Elucidation of a 36 kDa Muramidase/Transglycosylase Using wARP

The X-ray structure of the 36kDa soluble lytic transglycosylase from Escherichia coli has been determined starting with the multiple isomorphous replacement method with inclusion of anomalous scattering at 2.7 Å resolution. Subsequently, before any model building was carried out, phases were extended to 1.7 Å, resolution with the weighted automated refinement procedure wARP, which gave a dramatic improvement in the phases. The electron-density maps from wARP were of outstanding quality for both the main chain and the side chains of the protein, which allowed the time spent on the tracing, interpretation and building of the X-ray structure to be substantially shortened. The structure of the soluble lyric transglycosylase was refined at 1.7 Å, resolution with X-PLOR to a final crystallographic R factor of 18.9%. Analysis of the wARP procedure revealed that the use of the maximum-likelihood refinement in wARP gave much better phases than least-squares refinement, provided that the ratio of reflections to protein atom parameters was approximately 1.8 or higher. Furthermore, setting aside 5% of the data for an Rfree test set had a negative effect on the phase improvement. The mean WwARP, a weight determined at the end of the wARP procedure and based on the variance of structure factors from six individually refined wARP models, proved to be a better indicator than the Rfree factor to judge different phase improvement protocols. The elongated Slt35 structure has three domains named the alpha, beta and core domains. The alpha domain contains mainly α-helices, while the beta domain consists of a five-stranded antiparallel β-sheet flanked by a short α-helix. Sandwiched between the alpha and beta domains is the core domain, which bears some resemblance to the fold of the catalytic domain of the previously elucidated 70 kDa soluble lytic transglycosylase from E. coli. The putative active site is at the bottom of a large deep groove in the core domain.

A Triangular Tessellation Scheme for the Adsorption Free Energy at the Liquid-Liquid Interface: Towards Non-Convex Patterned Colloids

We introduce a new numerical technique, namely triangular tessellation, to calculate the free energy associated with the adsorption of a colloidal particle at a flat interface. The theory and numerical scheme presented here are sufficiently general to handle non-convex patchy colloids with arbitrary surface patterns characterized by a wetting angle, e.g., amphiphilicity. We ignore interfacial deformation due to capillary, electrostatic, or gravitational forces, but the method can be extended to take such effects into account. It is verified that the numerical method presented is accurate and sufficiently stable to be applied to more general situations than presented in this paper. The merits of the tessellation method prove to outweigh those of traditionally used semi-analytic approaches, especially when it comes to generality and applicability.Comment: 21 pages, 11 figures, 0 table

On the Detectability of the Hydrogen 3-cm Fine Structure Line from the EoR

A soft ultraviolet radiation field, 10.2 eV < E <13.6 eV, that permeates neutral intergalactic gas during the Epoch of Reionization (EoR) excites the 2p (directly) and 2s (indirectly) states of atomic hydrogen. Because the 2s state is metastable, the lifetime of atoms in this level is relatively long, which may cause the 2s state to be overpopulated relative to the 2p state. It has recently been proposed that for this reason, neutral intergalactic atomic hydrogen gas may be detected in absorption in its 3-cm fine-structure line (2s_1/2 -> 2p_3/2) against the Cosmic Microwave Background out to very high redshifts. In particular, the optical depth in the fine-structure line through neutral intergalactic gas surrounding bright quasars during the EoR may reach tau~1e-5. The resulting surface brightness temperature of tens of micro K (in absorption) may be detectable with existing radio telescopes. Motivated by this exciting proposal, we perform a detailed analysis of the transfer of Lyman beta,gamma,delta,... radiation, and re-analyze the detectability of the fine-structure line in neutral intergalactic gas surrounding high-redshift quasars. We find that proper radiative transfer modeling causes the fine-structure absorption signature to be reduced tremendously to tau< 1e-10. We therefore conclude that neutral intergalactic gas during the EoR cannot reveal its presence in the 3-cm fine-structure line to existing radio telescopes.Comment: 7 pages, 4 figures, MNRAS in press; v2. some typos fixe