Comment on 'Tumour antigen expression in hepatocellular carcinoma in a low-endemic western area'

We comment on the recent study by Sideras et al (2015) that combines tissue microarrays (TMAs) and immunohistochemistry to investigate the expression pattern of 15 antigens belonging to different categories, including cancer-testis antigens and oncofetal proteins in hepatocellular carcinoma (HCC). Because current therapies for HCC are far from ideal (Ilan, 2014) and immunotherapy has been suggested as a potential therapeutic option, the Authors aimed at identifying a panel of biologically relevant tumour antigens with broad expression in a western European population of HCC patients and specific expression in the tumour tissue with no, or little, expression in surrounding non- tumoral tissue (Sideras et al., 2015)

The structure of gravel-bed flow with intermediate submergence: a laboratory study

The paper reports an experimental study of the flow structure over an immobile gravel bed in open channel at intermediate submergence, with particular focus on the near-bed region. The experiments consisted of velocity measurements using three-component (stereoscopic) Particle Image Velocimetry (PIV) in near-bed horizontal plane and two-component PIV in three vertical planes that covered three distinctly different hydraulic scenarios where the ratio of flow depth to roughness height (i.e., relative submergence) changes from 7.5 to 10.8. Detailed velocity measurements were supplemented with fine-scale bed elevation data obtained with a laser scanner. The data revealed longitudinal low-momentum and high-momentum "strips'' in the time-averaged velocity field, likely induced by secondary currents. This depth-scale pattern was superimposed with particle-scale patches of flow heterogeneity induced by gravel particle protrusions. A similar picture emerged when considering second-order velocity moments. The interaction between the flow field and gravel-bed protrusions is assessed using cross correlations of velocity components and bed elevations in a horizontal plane just above gravel particle crests. The cross correlations suggest that upward and downward fluid motions are mainly associated with upstream-facing and lee sides of particles, respectively. Results also show that the relative submergence affects the turbulence intensity profiles for vertical velocity over the whole flow depth, while only a weak effect, limited to the near-bed region, is noticed for streamwise velocity component. The approximation of mean velocity profiles with a logarithmic formula reveals that log-profile parameters depend on relative submergence, highlighting inapplicability of a conventional "universal'' logarithmic law for gravel-bed flows with intermediate submergence

Permanent monitoring of thin structures with low-cost devices

Recently, structural monitoring technology invested in methodologies that give direct information on structures' stress state. Optic fibers, strain gauges, pressure cells give real-time data on the stress condition of a structural element, often determining the area where peak stresses have been reached, with a clear advantage over other less direct monitoring methodologies, such as, e.g., the use of accelerometers and inverse analysis to estimate internal forces. In addition, stresses can be recorded in a data log for analysis after a loading event, as well as for taking into account the lifelong stress state of the structure. Beams and columns of a reinforced concrete frame can be effectively monitored for flexural loads. Differently, thin shells are most of their lifespan under membrane regime, and, when properly designed, they rarely move to the bending regime. Our proposal is to monitor the stress in thin structures by small-sized low- cost devices able to record the stress history at key locations, sending alerts when necessary, with the aim of ensuring safety against the risk of collapse, or simply to perform maintenance/repairing activities. Such devices are realized with cheap off-the-shelf electronics and traditional strain gauges. The application examples are given as laboratory tests performed on a reinforced concrete plate, a masonry panel, and a steel beam. Results shows that the permanent monitoring control of stresses can be conveniently carried out on new structures using low-cost devices of the type we designed and realized in-house

IoT sensors for modern structural health monitoring. A new frontier

The problem of determining the structural safety level of buildings and civil engineering infrastructures (CEIs) is raising growing concern worldwide. Most of the reinforced concrete constructions have a design life not greater than 100 years, and today it is necessary to face the problem of assessing their level of safety and structural integrity. Such problem is even more pressing when a construction is subjected to extreme environmental conditions. The long-term goal of this study is the realization of wireless low- cost devices, and a data management software, for the structural health monitoring of buildings and CEIs, with remotely controlled sensors embedded in, or installed on, the structural elements, to measure stresses together with accelerations. Once equipped with such system, each construction can become part of the Internet of Things, permitting users and authorities to be alerted in case structural safety is diminished or compromised. A crucial aspect is the unaltered preservation of measurement data over time, which cannot just rely on third parties, and for which it is necessary the exploitation of suitable data-protection technologies. This study have been carried out by experimental testing and validation, both in lab and on site, of the monitoring devices designed and realized. Results show that it is possible to realize low-cost monitoring systems, and related installation techniques, for integration in every new or existing buildings and CEIs

Sperm protein 17 is expressed in human nervous system tumours

BACKGROUND: Human sperm protein 17 (Sp17) is a highly conserved protein that was originally isolated from a rabbit epididymal sperm membrane and testis membrane pellet. It has recently been included in the cancer/testis (CT) antigen family, and shown to be expressed in multiple myeloma and ovarian cancer. We investigated its immunolocalisation in specimens of nervous system (NS) malignancies, in order to establish its usefulness as a target for tumour-vaccine strategies. METHODS: The expression of Sp17 was assessed by means of a standardised immunohistochemical procedure [(mAb/antigen) MF1/Sp17] in formalin-fixed and paraffin embedded surgical specimens of NS malignancies, including 28 neuroectodermal primary tumours (6 astrocytomas, 16 glioblastoma multiforme, 5 oligodendrogliomas, and 1 ependymoma), 25 meningeal tumours, and five peripheral nerve sheath tumours (4 schwannomas, and 1 neurofibroma),. RESULTS: A number of neuroectodermal (21%) and meningeal tumours (4%) were found heterogeneously immunopositive for Sp17. None of the peripheral nerve sheath tumours was immunopositive for Sp17. The expression pattern was heterogeneous in all of the positive samples, and did not correlate with the degree of malignancy. CONCLUSION: The frequency of expression and non-uniform cell distribution of Sp17 suggest that it cannot be used as a unique immunotherapeutic target in NS cancer. However, our results do show the immunolocalisation of Sp17 in a proportion of NS tumour cells, but not in their non-pathological counterparts. The emerging complex function of Sp17 makes further studies necessary to clarify the link between it and immunopositive cells

Cancer initiation and progression: an unsimplifiable complexity

BACKGROUND: Cancer remains one of the most complex diseases affecting humans and, despite the impressive advances that have been made in molecular and cell biology, how cancer cells progress through carcinogenesis and acquire their metastatic ability is still widely debated. CONCLUSION: There is no doubt that human carcinogenesis is a dynamic process that depends on a large number of variables and is regulated at multiple spatial and temporal scales. Viewing cancer as a system that is dynamically complex in time and space will, however, probably reveal more about its underlying behavioural characteristics. It is encouraging that mathematicians, biologists and clinicians continue to contribute together towards a common quantitative understanding of cancer complexity. This way of thinking may further help to clarify concepts, interpret new and old experimental data, indicate alternative experiments and categorize the acquired knowledge on the basis of the similarities and/or shared behaviours of very different tumours

preliminary results of principe predictors of resistance to immunotherapy with nivolumab niv study in advanced pretreated non small cell lung cancer apnsclc investigating the role of an immune genomic signature igs including jak2 jak3 pias4 ptpn2 stat3 ifnar2 alterations

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