Prevalence of Cytomegalovirus antibodies and CD4 level among HIV/AIDS patients attending Specialist Hospital, Sokoto, Nigeria

Background: Cytomegalovirus (CMV) is member is a member of the family Herpesviridae. It establishes latent state following primary infection, reactivating when there are changes in immune status. It is a virus of paradoxes and can be a potential killer or a silent lifelong companion. CMV infection is a common opportunistic viral infection among HIV/AIDS patients.Aim: This study therefore aimed at Studying “Prevalence of Cytomegalovirus (IgG, IgM) antibodies and CD4 level among HIV Positive Patients Attending Specialist Hospital, Sokoto.Methodology: A total of 90 HIV patients and 30 healthy individuals were enrolled. Blood was collected and the samples were screened serologically for IgG, IgM, IgG/IgM antibodies and CD4 levels were estimated. Results: The results showed that out of 90 HIV patients, 15(16.6%) were IgG reactive, 6(7.1%) IgM reactive and 5(5.88%) were IgG/IgM reactive. For the Controls out of 30, 3(10%) IgG positive, 3(10%) IgM and2 (6.6%) IgG/IgM positive with p=0.358, 0.689, 0.824 respectively. This shows that there no statistically significant difference between CMV infection in HIV patients and Healthy individuals. The CD4 level was estimated for test and control, Mean 514.92±202.36 and 997.84±145 respectively. P value is 0.000 which shows that there is statistically significant difference between CD4 level in the test and control. Similarly, the association between CD4 level and CMV antibodies (IgG, IgM and IgG/IgM) was obtained using Chi square and the p values were 0.057, 0.968 and 0.464. This indicated that there is no statistically significant association between CD4 level and CMV infections.Conclusion: Although, the study indicated that CMV infection among HIV positive cases is statistically not significant, but the case study reveals the need of further investigation to highlight the severity of the problem. It will help in better management of the HIV cases with early diagnosis of CMV antibodies in the patients

Integrated atomistic process and device simulation of decananometre MOSFETs

In this paper we present a methodology for the integrated atomistic process and device simulation of decananometre MOSFETs. The atomistic process simulations were carried out using the kinetic Monte Carlo process simulator DADOS, which is now integrated into the Synopsys 3D process and device simulation suite Taurus. The device simulations were performed using the Glasgow 3D statistical atomistic simulator, which incorporates density gradient quantum corrections. The overall methodology is illustrated in the atomistic process and device simulation of a well behaved 35 nm physical gate length MOSFET reported by Toshiba

Detection of Multidrug-Resistant Acinetobacter baumannii among Gram-Negative Bacteria Isolated from Clinical Samples

Acinetobacter baumannii is an aerobic, Gram -negative cocco-bacilli, non-fermentative, non-motile, and non-fastidious organism belonging to the genus Acinetobacter. The A. baumannii has emerged as a worldwide nosocomial pathogen causing about 80%25 of nosocomial infections comprising ventilator-acquired pneumonia, bacteremia, meningitis, urinary tract infections, skin and soft tissues infections associated with high mortality rate of approximately 63.3%25. Although literature shows sufficient information about the drug resistant A. baumannii, there has been inadequate reports on the antibiotic resistance level of this bacterium in the study area. The aim of this research was to detect Multidrug-resistant A. baumannii isolates among Gram-negative bacteria isolated from Federal Teaching Hospital, Gombe, Nigeria. A total of 1008 clinical samples were collected and cultured on MacConkey agar and Blood agar plates at 37o C for 18-24 hours. Following the incubation period, discrete colonies obtained were subjected to Gram staining. The Gram-negative isolates were identified based on conventional biochemical tests with further use of VITEK 2 COMPACT (BioMérieux, France) for confirmation of A. baumannii amongst the Gram-negative organisms. The results obtained showed that 263 Gram-negative organisms were isolated. A. baumannii accounted for 8.5%25 prevalence. Most of the A. baumannii isolated were from the male patients (75%25) within the age range of 33-48 years. Antibiotic susceptibility test using Kirby Bauer method in accordance with CLSI guidelines was done on 20 A. baumannii isolates. The isolates were more sensitive to levofloxacin (60%25), followed by Gentamicin (55%25), then Ciprofloxacin and Tetracycline (50%25) respectively. High level of resistance to Ceftriaxone (80%25), Cefepime (75%25), Ceftazidime (65%25), Piperacillin-Tazobactam (55%25), Ampicillin%252FSulbactam (60%25), Tigecycline (60%25), Meropenem (55%25) and Amikacin (60%25). This study revealed that 15 (75%25) of the A. baumannii were found to be multidrug-resistant. Therefore, antibiotic stewardship is necessary to combat further dissemination of this organism

Comparative study of the level of bacterial/helminths contamination of vegetables produced from polluted and unpolluted irrigation site of Kano

A comparative study was contaminations of five major crops (Lactuca veriso, Darcus carota, Solanum lycopersicum, Phylanthus amarus, Alium cepa) produced from polluted (industrial/residential effluents) Sharada and unpolluted (tube well water)Yarimawa using the method described by FAO, (1979). The result shows that the five crops, produced with polluted water viz, Lactuca veriso, Darcus carota, Solanum lycopersicum Phylanthus amarus, Alium cepa, had mean aerobic plate count of 2.0 x 102 cfu/g, 8.40 x 102 cfu/g , 1.13 x 103 cfu/g , 5.8 x 104 cfu/g cfu/g, 5.7 x 101 cfu/g, respectively, and all the five crops had mean coliform count of 180 MPN/g, while that of unpolluted water had mean aerobic plate count of of 1.22 x 102 cfu/g 5.1 x 102 cfu/g , 1.4 x 102 cfu/g, 1.43 x 102 cfu/g, 1.32 x 101 cfu/g respectively, and had mean coliform count of 30, 21,21, 21 and 21MPN/g respectively. The organisms isolated in all the crops produced with polluted water were E. coli, Salmonella sp, Shigella sp, S. aureus, Ascaris egg, Strongloides and Ancylostoma while E. coli, Shigella sp, S. aureus, wer the organisms isolated in the crops produced with unpolluted water, only Lactuca verosa and Solanum lycopersicum, shows the presence of helmiths in the crops produced with the unpolluted water.Key words: Kano, Bacteria, Helminth, effluent, Yarimawa, Sharad

Effect of Dietary Replacement of Maize with Yam and Irish potato peel meals on the Growth and Economic Performance of Growing Rabbits

An eight-week feeding trial was conducted to investigate the effect of feeding yam and irish potato peel meals on the growth performance and economic analysis of cross-bred (Dutch x New Zealand white) growing rabbits aged between five to seven weeks. Thirty rabbits were randomly allocated to six dietary treatments with five rabbits per treatment. Diets 1, 2, 3, 4, 5 and 6 contained 100% maize, 50% maize and 50% yam peel meal, 50% maize and 50% irish potato peel meal, 50% yam peel meal and 50% irish potato peel meal, 100% yam peel meal and 100% irish potato peel meal, respectively. The response showed that the growth parameters were not significantly (P>0.05) different among the treatment groups except mean final body weight which was significantly (P>0.05) different. The mean final body weight of rabbits fed T3 diet (50% maize and 50% irish potato peel meal) was significantly (P< 0.05) higher than those fed T2 (50% maize and 50% yam peel meal) and T6 (100% irish potato peel meal) diets, but were similar (P>0.05) to other diets. However, the lowest mean final body weight was obtained in rabbits fed T6 (100% irish potato peel meal) diet. The cost per kg feed decreased progressively as the levels of yam and irish potato peel meals increased in the diets. The cost per kg gain also declined from  ₦ 249.59 in T1 (100% maize) to ₦ 181.95 in T4 (50% yam peel meal and 50% irish potato peel meal), which showed the best cost per kg weight gain. The study demonstrated that the yam and irish potato peel meals can replace maize up to 100% as energy source for growing rabbits without adverse effect on performance.Keywords: Growth Performance, Yam and Irish Potato peel meals, Economic Analysi

PM3 and DFT Computational Studies of the Reaction Mechanism of Formaldehyde and Isoleucine

The reaction of formaldehyde and Isoleucine was studied using semi-empirical and density functional theory methods. Possible reactive sites are proposed and reaction mechanism postulated. It was found that the Isoleucine nitrogen attacks the carbonyl carbon of formaldehyde and forms a methylol intermediate that undergoes a condensation with another Isoleucine to produce a Methylenediisoleucine through a methylene bridge (cross-linking). The enthalpies of the reaction are -78.79 kJ/mol and -39.14kJ/mol for PM3 and DFT respectively also ΔSo and ΔGo, for the PM3 and DFT studies predicted. The reaction was found to be exothermic and second order

Highly prevalent hyperuricaemia is associated with adverse clinical outcomes among Ghanaian stroke patients: An observational prospective study

Background: Although a direct causal relationship between hyperuricaemia and stroke continues to be debated, strong associations between serum uric acid (SUA) and cerebrovascular disease exist. Very few studies have been conducted to evaluate the frequency and association between this potentially modifiable biomarker of vascular risk and stroke in sub-Saharan Africa. Therefore the aim of this study was to examine the association between hyperuricaemia and the traditional risk factors and the outcomes of stroke in Ghanaian patients.Methods: In this prospective observational study, 147 patients presenting with stroke at a tertiary referral centre in Ghana were consecutively recruited. Patients were screened for vascular risk factors and SUA concentrations measured after an overnight fast. Associations between hyperuricaemia and stroke outcomes were analysed using Kaplan-Meier and Cox proportional hazards regression analysis.Results: The frequency of hyperuricaemia among Ghanaian stroke patients was 46.3%. Non-significant associations were observed between hyperuricaemia and the traditional risk factors of stroke. SUA concentration was positively correlated with stroke severity and associated with early mortality after an acute stroke with unadjusted hazards ratio of 2.3 (1.4 - 4.2, p=0.001). A potent and independent dose-response association between increasing SUA concentration and hazard of mortality was found on Cox proportional hazards regression, aHR (95% CI) of 1.65 (1.14-2.39), p=0.009 for each 100μmol/l increase in SUA.Conclusions: Hyperuricaemia is highly frequent and associated with adverse functional outcomes among Ghanaian stroke patients. Further studies are warranted to determine whether reducing SUA levels after a stroke would be beneficial within our setting.Key words: Hyperuricaemia, stroke, mortality, Ghan

Synthesis and characterization of zinc oxide nanoparticles (ZnO-NPs) for biodiesel production using waste frying oil (WFO)

In this research, zinc oxide nanoparticles (ZnO-NPs) were synthesized by simple solution-based approach using zinc nitrate  [Zn(NO3)2.6H2O] and sodium hydroxide (NaOH) as precursor. The Synthesized ZnO-NPs were characterized by XRF, XRD and TGA methods. XRD method confirmed the formation of nanosized zinc oxide particles in the size range of 13–47 nm. XRF and TGA were used to determine the elemental composition and thermal stability of ZnO-NPs, respectively. The ZnO-NPs were used as catalyst in the production of biodiesel from waste frying oil (WFO) via transesterification method. The transesterification process yielded 97.8 % (w/w) WFO biodiesel. The results of the fuel properties revealed that, all the parameters tested are within the ASTM limits, indicating that the biodiesel produced could be used as an alternative diesel fuel

Extadigits: an unusual presentation

Extra digit is a common congenital anomaly in our environment which usually affect the hands and occasionally the feet but very rarely both. A five months old male infant presented with accessory digits of the both hands and feet, with two extra digits on the left hand and one on the right hand, right foot and left foot. The extra digits were well developed, with normal range of motion, good capillary refill and intact sensation. General examination revealed an otherwise healthy child with no associated congenital malformations. The treatment modality used was surgical removal of the extra digits and reconstruction of any associated anomalies in the remaining ray such as longitudinal epiphyseal bracket. After the surgery the patients is no longer experience difficulty with fitting gloves and shoes as well as discrimination among peer groups in his future life

Wound Healing, Antioxidants and Toxicological Properties of Root Extracts of Kigelia africana (Lam.) Benth

The root extracts of Kigelia africana were screened for antibacterial and wound healing properties, using hole-plate bioassay and excision wound model on rats, respectively. Catalase activity, glutathione level and lipid peroxidation were assayed in the granulated tissues and liver homogenates. Chemical compositions of the root were determined using standard methods. Complete wound healing was observed on day 16 in group administered with 120mg/ml and on the 19th day in groups administered 90 and 60mg/ml of the extract. Clinical features indicate redness, scab formation, exudations and some other typical changes. The control and antibiotic treated groups show more redness compared to third day. The hydrolytic and organic solvent fractions show significant (p< 0.05) inhibitory activities on woun