2,033 research outputs found

    Observation of a Free-Shercliff-Layer Instability in Cylindrical Geometry

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    We report on observations of a free-Shercliff-layer instability in a Taylor-Couette experiment using a liquid metal over a wide range of Reynolds numbers, Re∼103−106Re\sim 10^3-10^6. The free Shercliff layer is formed by imposing a sufficiently strong axial magnetic field across a pair of differentially rotating axial endcap rings. This layer is destabilized by a hydrodynamic Kelvin-Helmholtz-type instability, characterized by velocity fluctuations in the r−θr-\theta plane. The instability appears with an Elsasser number above unity, and saturates with an azimuthal mode number mm which increases with the Elsasser number. Measurements of the structure agree well with 2D global linear mode analyses and 3D global nonlinear simulations. These observations have implications for a range of rotating MHD systems in which similar shear layers may be produced.Comment: 5 pages, 4 figure

    Pluripolarity of Graphs of Denjoy Quasianalytic Functions of Several Variables

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    In this paper we prove pluripolarity of graphs of Denjoy quasianalytic functions of several variables on the spanning se

    Metagenome and Metatranscriptome Analyses Using Protein Family Profiles

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    Analyses of metagenome data (MG) and metatranscriptome data (MT) are often challenged by a paucity of complete reference genome sequences and the uneven/low sequencing depth of the constituent organisms in the microbial community, which respectively limit the power of reference-based alignment and de novo sequence assembly. These limitations make accurate protein family classification and abundance estimation challenging, which in turn hamper downstream analyses such as abundance profiling of metabolic pathways, identification of differentially encoded/expressed genes, and de novo reconstruction of complete gene and protein sequences from the protein family of interest. The profile hidden Markov model (HMM) framework enables the construction of very useful probabilistic models for protein families that allow for accurate modeling of position specific matches, insertions, and deletions. We present a novel homology detection algorithm that integrates banded Viterbi algorithm for profile HMM parsing with an iterative simultaneous alignment and assembly computational framework. The algorithm searches a given profile HMM of a protein family against a database of fragmentary MG/MT sequencing data and simultaneously assembles complete or near-complete gene and protein sequences of the protein family. The resulting program, HMM-GRASPx, demonstrates superior performance in aligning and assembling homologs when benchmarked on both simulated marine MG and real human saliva MG datasets. On real supragingival plaque and stool MG datasets that were generated from healthy individuals, HMM-GRASPx accurately estimates the abundances of the antimicrobial resistance (AMR) gene families and enables accurate characterization of the resistome profiles of these microbial communities. For real human oral microbiome MT datasets, using the HMM-GRASPx estimated transcript abundances significantly improves detection of differentially expressed (DE) genes. Finally, HMM-GRASPx was used to reconstruct comprehensive sets of complete or near-complete protein and nucleotide sequences for the query protein families. HMM-GRASPx is freely available online from http://sourceforge.net/projects/hmm-graspx

    Sulphate-controlled Diversity of Subterranean Microbial Communities over Depth in Deep Groundwater with Opposing Gradients of Sulphate and Methane

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    The groundwater system in Olkiluoto, Finland, is stratified with a mixing layer at a depth of approximately 300m between sulphate-rich, methane-poor and sulphate-poor, methane-rich groundwaters. New sequence library data obtained by 454 pyrotag sequencing of the v4v6 16S rDNA region indicated that sulphate-reducing bacteria (SRB) dominated the mixing layer while SRB could not be detected in the deep sulphate-poor groundwater samples. With the indispensable support of the sequence data, it could be demonstrated that sulphate was the only component needed to trigger a very large community transition in deep sulphate-poor, methane-rich groundwater from a non-sulphate-reducing community comprising Hydrogenophaga, Pseudomonas, Thiobacillus, Fusibacter, and Lutibacter to a sulphate-reducing community with Desulfobacula, Desulfovibrio, Desufobulbaceae, Desulfobacterium, Desulfosporosinus, and Desulfotignum. Experiments with biofilms and planktonic microorganisms in flow cells under in situ conditions confirmed that adding sulphate to the sulphate-poor groundwater generated growth of cultivable SRB and detectable SRB-related sequences. It was also found that the 16S rDNA diversity of the biofilms was conserved over 103 d and that there was great similarity in diversity between the microorganisms in the biofilms and in the flowing groundwater. This work demonstrates that the presence/absence of only one geochemical parameter, i.e., sulphate, in the groundwater significantly influenced the diversity of the investigated subterranean microbial community

    On searches for gravitational waves from mini creation event by laser interferometric detectors

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    As an alternative view to the standard big bang cosmology the quasi-steady state cosmology(QSSC) argues that the universe was not created in a single great explosion; it neither had a beginning nor will it ever come to an end. The creation of new matter in the universe is a regular feature occurring through finite explosive events. Each creation event is called a mini-bang or, a mini creation event(MCE). Gravitational waves are expected to be generated due to any anisotropy present in this process of creation. Mini creation event ejecting matter in two oppositely directed jets is thus a source of gravitational waves which can in principle be detected by laser interferometric detectors. In the present work we consider the gravitational waveforms propagated by linear jets and then estimate the response of laser interferometric detectors like LIGO and LISA

    AICLE y la educación se unen: La encrucijada del multilingüismo

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    It has been only 7 years since both the first issue of the Latin American Journal of Content and Language Integrated Learning (LACLIL) was published and the 1st CLIL Symposium was held at the Universidad de La Sabana in Chía, Colombia in 2008. Over this period, the pace of debate on education and languages has only increased, and growing interest in CLIL (content and language integrated learning) approaches continues to raise new questions for researchers, teachers, policy makers, and learners—indeed, society at large—about the needs for, and challenges of, integrating content and additional (or even first) languages in the classroom and beyon
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