Kultur Sel Otak Dan Mata Ikan Kerapu (Chromileptes Altivelis) Untuk Replikasi Viral Nervous Necrosis (Vnn) [Cell Line Chromileptes Alttivelis From Enchepalon and Retina for Viral Nervous Necrosis (Vnn) Replication]

The experiment was deal with propagat cell line from encephalon and retina of grouper Chromileptes altivelis.The cell was isolate propagation of method described following by Chen et al.(1982) from carp and crussian carp fin. The cell line from enchepalon and retina of grouper grew very fast and heavy in medium L-1S.The cell line were then used for viral replication giving,isolated virus with high pathogenicity indicated by mortal effect in short time after administration. The preservation of cell line is in 10% glycerol under -80°C for one year. Thawing will be done every 3 months to ensure the viability of the cell

Guía para la presentación de monografías de postgrado: un aporte desde la biblioteca de Facultad de Medicina, Universidad de la República

This document has been prepared with the aim of contributing to the development of the postgraduate studies. It covers aspects of structure and style by international standards, to achieve normalization in the presentation of the works. It is considered that the proper use of information and the correct form in which this one shoud be presented, not only gives sample of the professional and intellectual solidity of the author, but it directly affects the quality of the publication

Apelin enhances the effects of Fusobacterium nucleatum on periodontal ligament cells in vitro

This study aimed to explore effects of Fusobacterium nucleatum with or without apelin on periodontal ligament (PDL) cells to better understand pathomechanistic links between periodontitis and obesity. First, the actions of F. nucleatum on COX2, CCL2, and MMP1 expressions were assessed. Subsequently, PDL cells were incubated with F. nucleatum in the presence and absence of apelin to study the modulatory effects of this adipokine on molecules related to inflammation and hard and soft tissue turnover. Regulation of apelin and its receptor (APJ) by F. nucleatum was also studied. F. nucleatum resulted in elevated COX2, CCL2, and MMP1 expressions in a dose- and time-dependent manner. Combination of F. nucleatum and apelin led to the highest (p < 0.05) expression levels of COX2, CCL2, CXCL8, TNF-α, and MMP1 at 48 h. The effects of F. nucleatum and/or apelin on CCL2 and MMP1 were MEK1/2- and partially NF-κB-dependent. The combined effects of F. nucleatum and apelin on CCL2 and MMP1 were also observed at protein level. Moreover, F. nucleatum downregulated (p < 0.05) the apelin and APJ expressions. In conclusion, obesity could contribute to periodontitis through apelin. The local production of apelin/APJ in PDL cells also suggests a role of these molecules in the pathogenesis of periodontitis

Apelin Enhances the Effects of Fusobacterium nucleatum on Periodontal Ligament Cells In Vitro.

This study aimed to explore effects of Fusobacterium nucleatum with or without apelin on periodontal ligament (PDL) cells to better understand pathomechanistic links between periodontitis and obesity. First, the actions of F. nucleatum on COX2, CCL2, and MMP1 expressions were assessed. Subsequently, PDL cells were incubated with F. nucleatum in the presence and absence of apelin to study the modulatory effects of this adipokine on molecules related to inflammation and hard and soft tissue turnover. Regulation of apelin and its receptor (APJ) by F. nucleatum was also studied. F. nucleatum resulted in elevated COX2, CCL2, and MMP1 expressions in a dose- and time-dependent manner. Combination of F. nucleatum and apelin led to the highest (p < 0.05) expression levels of COX2, CCL2, CXCL8, TNF-α, and MMP1 at 48 h. The effects of F. nucleatum and/or apelin on CCL2 and MMP1 were MEK1/2- and partially NF-κB-dependent. The combined effects of F. nucleatum and apelin on CCL2 and MMP1 were also observed at protein level. Moreover, F. nucleatum downregulated (p < 0.05) the apelin and APJ expressions. In conclusion, obesity could contribute to periodontitis through apelin. The local production of apelin/APJ in PDL cells also suggests a role of these molecules in the pathogenesis of periodontitis

RBX: The new X-band SAR system from INTA

Revista oficial de la Asociación Española de Teledetección[EN] This paper describes the characteristics and the first results of the RBX system, the new airborne Synthetic Aperture Radar developed at Spanish National Institute of Aerospace Technology. The new system supports submetric resolution with interferometric and polarimetric capabilities. The system architecture has been designed taking into account an easy upgrade ability and the quality of the acquired data, including multiple internal calibration loops.[ES] Este artículo describe las características y los primeros resultados del sistema RBX, nuevo radar de apertura sintética aerotransportado que ha sido desarrollado en el Instituto Nacional de Técnica Aeroespacial. El nuevo sistema soporta resoluciones submétricas con capacidades interferométricas y polarimétricas. La arquitectura del sistema se ha diseñado teniendo en cuenta la facilidad de incorporar mejoras y la calidad de los datos adquiridos, incluyendo múltiples lazos de calibración interna.Larrañaga, J. R., De Porras Bernácer, R., Del Castillo, J., Gimeno, N., Aguilar, P., Baquero, A. M., . . . Sánchez, S. (2014). RBX: The new X-band SAR system from INTA. [RBX: El nuevo radar SAR en banda X del INTA] Revista De Teledeteccion, (41), 89-96. doi:10.4995/raet.2014.2285SWORD899641G. Bonin, P. Dreuillet. 2008. The new ONERA airborne SAR system SETHI. EUSAR, Dresden, Germany.Rodriguez, M. G. (2008). Design of a Coherent Generation and Acquisition System at 1,2GHz. 2008 4th Southern Conference on Programmable Logic. doi:10.1109/spl.2008.4547745Gonzalez Bonilla, M. J., Gomez Miguel, B., Cuerda Muñoz, J. M., Larrañaga Sudupe, J. R., Garcia Rodriguez, M., 2009. INTASAR program. Proceedings IGARSSHORN, RALF; NOTTENSTEINER, ANTON; SCHEIBER, ROLF. 2008. F-SAR – DLR's advanced airborne SAR system onboard DO228. Proceedings EUSARMoreira, A., & Yonghong Huang. (1994). Airborne SAR processing of highly squinted data using a chirp scaling approach with integrated motion compensation. IEEE Transactions on Geoscience and Remote Sensing, 32(5), 1029-1040. doi:10.1109/36.31289

Ifosfamide/etoposide alternating with high-dose methotrexate: evaluation of a chemotherapy regimen for poor-risk osteosarcoma

Fifteen patients with relapsed osteosarcoma were treated with an intensive combination chemotherapy schedule. Ifosfamide 2.5 g m−2 daily and etoposide 150 mg m−2 daily coincidentally for 3 days and high-dose methotrexate 8 g m−2 (with folinic acid rescue) on days 10–14 in a planned 21-day cycle. Feasibility, toxicity and response to this alternative combination for the treatment of relapsed osteosarcoma was assessed. There were 98 evaluable cycles for toxicity and tolerability. The majority of cycles were well tolerated. Haematological toxicity of grade 3/4 (common toxicity criteria) was seen in all courses. Renal tubular loss of electrolytes, particularly magnesium, occurred in 71% of cycles. Thirteen per cent of cycles were repeated within 21 days and 61% within 28 days. In the thirteen patients evaluable for response, a partial response rate of 31% was seen after two cycles. However, patients with stable disease continued on therapy, and an overall consequent response rate of 62% was observed. Four patients were alive with no evidence of disease at 8–74 months. Three are alive with disease (at 8–19 months). There were six deaths, all disease related. This regimen exhibits an encouraging response rate in a group of children with poor prognosis disease, with a tolerable toxicity profile. © 1999 Cancer Research Campaig

New insights into the genetic etiology of Alzheimer's disease and related dementias

Characterization of the genetic landscape of Alzheimer's disease (AD) and related dementias (ADD) provides a unique opportunity for a better understanding of the associated pathophysiological processes. We performed a two-stage genome-wide association study totaling 111,326 clinically diagnosed/'proxy' AD cases and 677,663 controls. We found 75 risk loci, of which 42 were new at the time of analysis. Pathway enrichment analyses confirmed the involvement of amyloid/tau pathways and highlighted microglia implication. Gene prioritization in the new loci identified 31 genes that were suggestive of new genetically associated processes, including the tumor necrosis factor alpha pathway through the linear ubiquitin chain assembly complex. We also built a new genetic risk score associated with the risk of future AD/dementia or progression from mild cognitive impairment to AD/dementia. The improvement in prediction led to a 1.6- to 1.9-fold increase in AD risk from the lowest to the highest decile, in addition to effects of age and the APOE ε4 allele