Energy and technological refurbishment of the School of Architecture Valle Giulia, Rome

Modern architecture built in historical urban contexts represents a demanding issue when its energy efficiency should be improved. Indeed, the strongest efforts have to be made to maintain the architectural identity and its harmony with the surrounding cultural heritage. This study deals with the main building of the School of Architecture Valle Giulia in Rome, designed by Enrico Del Debbio in the 30’s. Further constraints are related to several interventions of airspace expansion starting from 1958 which involved the building starting from 1958. So, preservation would mean highlighting its historic change but, adapting the built environment to the contemporary users’ needs. As above-mentioned, the building belongs to the Valle delle Accademie, within the historic park of Villa Borghese, so that to acquire landscaping values. Those latter ones call for ulterior requirements when any new design process is conceived. The study provides a global renewal of the building accounting for the current low Indoor Environmental Quality in both summer and winter seasons and the lack of suitability to the contemporary University student’s needs. The interaction between building performance and HVAC systems was studied by collecting data and architectural surveys conducted by all the architects who modified the building. This procedure was chosen since thermo-physical investigations are considered destructive due to required perforations to identify the actual wall layers. Moreover, thermographic surveys were carried out to validate the modelled building response. The result of the study is the identification of viable interventions to improve the accessibility and fruition of the building as well as its energy performance. A specific cost-benefit analysis was done to prioritize the design options along with considering the measures needed to preserve all the architectural features and values

Micro-doppler-based in-home aided and unaided walking recognition with multiple radar and sonar systems

Published in IET Radar, Sonar and Navigation. Online first 21/06/2016.The potential for using micro-Doppler signatures as a basis for distinguishing between aided and unaided gaits is considered in this study for the purpose of characterising normal elderly gait and assessment of patient recovery. In particular, five different classes of mobility are considered: normal unaided walking, walking with a limp, walking using a cane or tripod, walking with a walker, and using a wheelchair. This presents a challenging classification problem as the differences in micro-Doppler for these activities can be quite slight. Within this context, the performance of four different radar and sonar systems – a 40 kHz sonar, a 5.8 GHz wireless pulsed Doppler radar mote, a 10 GHz X-band continuous wave (CW) radar, and a 24 GHz CW radar – is evaluated using a broad range of features. Performance improvements using feature selection is addressed as well as the impact on performance of sensor placement and potential occlusion due to household objects. Results show that nearly 80% correct classification can be achieved with 10 s observations from the 24 GHz CW radar, whereas 86% performance can be achieved with 5 s observations of sonar

Effects of Feed Additives and Mixed Eimeria Species Infection on Intestinal Microbial Ecology of Broilers

Evaluation of digestive microbial ecology is necessary to understand effects of growth-promoting feed. In the current study, the dynamics of intestinal microbial communities (MC) were examined in broilers fed diets supplemented with a combination of antibiotic (bacitracin methylene disalicylate) and ionophore (Coban 60), and diets containing 1 of 2 essential oil (EO) blends, Crina Poultry (CP) and Crina Alternate (CA). Five treatments were analyzed: 1) unmedicated uninfected control; 2) unmedicated infected control; 3) feed additives monensin (bacitracin methylene disalicylate) + monensin (Coban 60; AI); 4) EO blend CP; and 5) EO blend CA. Additives were mixed into a basal feed mixture, and EO were adjusted to 100 ppm. Chicks were infected by oral gavage at 19 d of age with Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Duodenal, ileal, and cecal samples were taken from 12 birds per treatment just before and 7 d after challenge; 2 samples each were pooled to give a final number of 6 samples total; and all pooled samples were frozen until used for DNA extraction. Denaturing gradient gel electrophoresis was used to examine PCR-amplified fragments of the bacterial 16S ribosomal DNA variable region. Results are presented as percentages of similarity coefficients (SC). Dendrograms of PCR amplicon or band patterns indicated MC differences due to intestinal location, feed additives, and cocci challenge. Essential oil blends CP and CA affected MC in all gut sections. Each EO had different effects over MC, and they differed in most instances from the AI group. The cocci challenge caused drastic MC population shifts in duodenal, ileal, and cecal sections (36.7, 55.4, and 36.2% SC, respectively). Diets supplemented with CP supported higher SC between pre- and postchallenge MC (89.9, 83.3, and 76.4%) than AI (81.8., 57.4, and 60.0%). We concluded that mixed coccidia challenge caused drastic shifts in MC. These EO blends modulated MC better than AI, avoiding drastic shifts after a mixed challenge

Responses of Coccidia-Vaccinated Broilers to Essential Oil Blends Supplementation up to Forty-Nine Days of Age

Coccidiosis control may become a greater problem as the use of growth-promoting antibiotics (GPA) and ionophores declines. Vaccination with live oocysts may turn into a popular alternative to the use of coccidiostats in broilers, although cocci vaccination is frequently linked to temporary lower performance in young flocks. This experiment evaluates the dietary supplementation of 2 specific essential oil (EO) blends (Crina Poultry and Crina Alternate), either as alternatives to GPA and ionophores (BMD + Coban) or as feed additives that help to improve the performance of cocci-vaccinated broilers. Live performance and lesion scores were observed. These 2 specific EO blends differ in their efficacy to promote growth. Chickens that were not cocci vaccinated and were fed Crina Poultry had better feed conversion ratio (FCR) than the unmedicated control treatment in the starter period. The same EO improved FCR in cocci-vaccinated birds in the finisher period in comparison to the negative control group, but those responses were not significantly different from other treatments or significant at 49 d of age. No significant differences were observed in lesion scores at 37 d. Diets supplemented with a GPA-ionophore combination consistently supported the best BW gain and FCR in each period and the entire grow-out period. No significant beneficial or deleterious effects on live performance were observed due to these specific EO blends in cocci-vaccinated broilers

Intestinal Microbial Ecology of Broilers Vaccinated and Challenged With Mixed Eimeria Species, and Supplemented with Essential Oil Blends

Intestinal microbiota is an important component in the development of defense mechanisms in the gut mucosa. This project determined the dynamics of intestinal microbial communities (MC) of broilers vaccinated at first day of age with live oocysts of Eimeria species and fed diets supplemented with 2 specific essential oil (EO) blends, Crina Poultry (CP) and Crina Alternate (CA). Five treatments were analyzed: 1) unmedicated-uninfected (UU) control; 2) unmedicated-infected (UI) control; 3) vaccinated with Advent cocci-vaccine and without feed additive (COV) supplements; 4) vaccinated with Advent and supplemented with CP; and 5) vaccinated with Advent and supplemented with CA. The EO blends were added at 100 ppm to the same basal diets. Chicks were gavage-infected at 19 d of age with Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Duodenal, ileal, and cecal samples were taken from 12 birds per treatment just before the infection and 7 d after the challenge, pooled in 6 samples, and frozen. Denaturing gradient gel electrophoresis was used to examine PCR-amplified fragments of the bacterial 16S ribosomal DNA variable region. Results are presented as percentages of similarity coefficients (SC). Dendrograms of amplicon patterns indicated MC differences due to intestinal location, feed additives, and cocci infection. The EO blends CP and CA did affect MC in all gut sections. The cocci-infection caused drastic MC population shifts in duodenal, ileal, and cecal sections (36.7, 55.4, and 36.2% SC, respectively). The CP-supplemented birds had higher SC between pre- and postchallenge MC in duodenal and ileal (73.3, 81.8%) than COV (66.4, 66.5%). However, COV broilers had the smallest changes in cecal MC after infection (79.5% SC). We concluded that cocci-vaccination causes small changes in intestinal MC, but challenge causes drastic shifts. The EO blend supplementation modulates MC in cocci-vaccinated broilers, avoiding drastic shifts after a mixed coccidia infection. Correlations between MC dynamics and host responses are discussed

Chelator free gallium-68 radiolabelling of silica coated iron oxide nanorods via surface interactions

The commercial availability of combined magnetic resonance imaging (MRI)/positron emission tomography (PET) scanners for clinical use has increased demand for easily prepared agents which offer signal or contrast in both modalities. Herein we describe a new class of silica coated iron–oxide nanorods (NRs) coated with polyethylene glycol (PEG) and/or a tetraazamacrocyclic chelator (DO3A). Studies of the coated NRs validate their composition and confirm their properties as in vivo T₂ MRI contrast agents. Radiolabelling studies with the positron emitting radioisotope gallium-68 (t1/2 = 68 min) demonstrate that, in the presence of the silica coating, the macrocyclic chelator was not required for preparation of highly stable radiometal-NR constructs. In vivo PET-CT and MR imaging studies show the expected high liver uptake of gallium-68 radiolabelled nanorods with no significant release of gallium-68 metal ions, validating our innovation to provide a novel simple method for labelling of iron oxide NRs with a radiometal in the absence of a chelating unit that can be used for high sensitivity liver imaging

Determinisability of One-Clock Timed Automata

The deterministic membership problem for timed automata asks whether the timed language recognised by a nondeterministic timed automaton can be recognised by a deterministic timed automaton. We show that the problem is decidable when the input automaton is a one-clock nondeterministic timed automaton without epsilon transitions and the number of clocks of the deterministic timed automaton is fixed. We show that the problem in all the other cases is undecidable, i.e., when either 1) the input nondeterministic timed automaton has two clocks or more, or 2) it uses epsilon transitions, or 3) the number of clocks of the output deterministic automaton is not fixed

A new approach in the use of SIT in determining the dependence on ionic strength of activity coefficients. Application to some chloride salts of interest in the speciation of natural fluids

AbstractThis paper describes a modified version of the SIT (Specific ion Interaction Theory) method and its use in determining the dependence on ionic strength of activity coefficients. In the new approach the interaction coefficients (e) are not constant but depend on ionic strength (I /mol kg-1) according to the simple relationship:e = e∞+ (e0 - e∞) / (l + 1)where e0 and are true constants for I→ 0 and l→ ∞, respectively. To check the two parameter SIT equation, we calculated e0 and for the activity coefficients of HCl, LiCl, NaCl, KCl, MgCl2, CaCl2 and SrCl2, in a wide ionic strength range (0.1 ≤ l/mol kg-1 ≤ 4.5, for KCl; 0.1 ≤ l/mol kg-1 ≤ 6, for HCl, LiCl, NaCl; 0.3 ≤ l/mol kg-1 ≤ 12, for SrCl2; 0.3 ≤ l/mol kg-1 ≤ 15, for MgCl2; 0.3 ≤ l/mol kg-1 ≤ 18, for CaCl2). Results show that the γ values calculated using this approach fit quite well over the whole I-range for all the electrolytes considered. Comparison is made with the analogous one parameter SIT equation. The temperature coefficients of inter..

Fourier independent component analysis of radar micro-Doppler features

The capability of discriminating radar targets exhibiting multiple moving parts has become of great interest for both aerospace and ground-based target recognition and analysis. In particular, helicopters and other targets with rotors, as for instance miniature Unmanned Aerial Vehicles, exhibit peculiar characteristics in the radar return that can be used for their recognition. In this paper a novel algorithm to address the problem of micro-Doppler signature unmixing is proposed, exploiting the signal separation capabilities of the Independent Component Analysis (ICA). The core of the algorithm is represented precisely by the use of the ICA procedure, that has been already proved to be a very effective technique for separating hidden information in mixtures of observations. ICA has been successfully employed in several applications such as wireless communications, radar beamforming, trace-gases unmixing and medical imaging processing. The helicopter's rotor blade signature unmixing from a multi-static radar system is considered as case study and results obtained through the application of ICA to simulated multi-component micro-Doppler signatures show the capability of the proposed approach to successfully accomplish the unmixing operation