Base composition of intact nucleic acid oligomers

Base composition of intact nucleic acid oligomer

Speculations on the postnatural: Restoration, accumulation, and sacrifice at the Salton Sea

Using a regional political ecology lens, this paper explores emerging geographies and politics of a “postnatural” ecomodernist turn in mainstream environmentalism. We examine the unfolding case of ecological restoration and renewable energy development at Southern California’s Salton Sea. Ambitious proposals to restore the massive, increasingly degraded lake (and finance restoration) by reengineering it as a hub for geothermal energy generation and high-tech green industry hinge upon the ambiguity and malleability of restoration in an environment long classified as postnatural. These plans coincide with a broader rush on renewable energy sites in the California desert, and mounting conflicts over water and land with legacy agro-industrial interests. The case illustrates significant problems within postnatural environmentalism. First, it demonstrates how theorizations of the postnatural can intersect with green capitalist projects of re(e)valuation and development, as the Sea’s managers manipulate environmental framings to support accumulation-minded projects, and accumulation imperatives swamp other functionalities of restoration. Meanwhile, despite the flourishing of postnatural discourses, the “pristine” is shown to do continued work as the Sea becomes a sacrifice zone for development deflected from better-protected spaces. This postnatural positioning has rendered the Salton Sea vulnerable to neoliberal austerity and speculation in ways that compromise its future existence

The Yamabe invariant for axially symmetric two Kerr black holes initial data

An explicit 3-dimensional Riemannian metric is constructed which can be interpreted as the (conformal) sum of two Kerr black holes with aligned angular momentum. When the separation distance between them is large we prove that this metric has positive Ricci scalar and hence positive Yamabe invariant. This metric can be used to construct axially symmetric initial data for two Kerr black holes with large angular momentum.Comment: 14 pages, 2 figure

Midwinter suppression of baroclinic storm activity on Mars: observations and models

Baroclinic instability and intense traveling wave activity on Mars is well known to occur in “storm zones” (Hollingsworth et al. 1996) close to the edge of the advancing or retreating polar ice cap. Such activity usually sets in during Martian fall and continues until the onset of the summer season when large-scale instability mostly ceases as the atmosphere is no longer baroclinically unstable. The stormy season is typically characterized by large-scale, zonally-propagating waves with zonal wavenumbers m = 1-3, the lower wavenumber modes typically penetrating to considerable altitude though may also be surface-intensified. As we show below, however, some observations suggest that this eddy activity does not persist uniformly throughout the autumn, winter and spring seasons, but appears to die down quite consistently within 10 sols or so either side of the winter solstice. This midwinter ‘solsticial pause’ appears to be a sufficiently consistent feature of each winter season in both hemispheres to be regarded as a significant feature of Martian climatology, and could affect a variety of aspects of Martian meteorology including global heat and momentum transport, occurrence of dust storms etc. A somewhat similar phenomenon has also been documented for the Earth (e.g. Nakamura 1992; Penny et al. 2010), especially in relation to seasonal variations in the north Pacific storm tracks. The cause of this phenomenon is still not well established, though suggested mechanisms include the effects of enhanced barotropic shear (the so-called ‘barotropic governor’ (James & Gray 1986) and interactions with topography over central Asia. In this presentation we examine evidence for this phenomenon in the assimilated record of Martian climate from the Thermal Emission Spectrometer on board the Mars Global Surveyor mission (MGSTES), in conjunction with the UK version of the LMD-Oxford-OU-IAA Mars GCM (Forget et al. 1999; Montabone et al. 2006; Lewis et al. 2007). This is further corroborated in other evidence from seasonal variations in the incidence of local and regional dust storms that owe their origin to circumpolar baroclinic storms. We also discuss the extent to which this ‘solsticial pause’ phenomenon is reproduced in stand-alone atmospheric models and present results of some simulations to test a number of hypotheses for its dynamical origin on Mars

Endosome pH measured in single cells by dual fluorescence flow cytometry: rapid acidification of insulin to pH 6

The acidification of various ligands was measured on a cell by cell basis for cell suspensions by correlated dual fluorescence flow cytometry. Mouse 3T3 cells were incubated with a mixture of fluorescein- and rhodamine-conjugated ligands, and the ratio of fluorescein and rhodamine fluorescence was used as a measure of endosome pH. The calibration of this ratio by both fluorometry and flow cytometry is described. Dual parameter histograms of average endosome pH per cell versus amount of internalization were calculated from this data, for samples in the absence and presence of chloroquine added to neutralize acidic cellular vesicles. The kinetics of acidification of insulin were measured and compared with previous results obtained with the chloroquine ratio technique. Rapid acidification of internalized ligand was observed both for insulin, which was mostly internalized via nonspecific pathways, and for alpha 2-macroglobulin, which was mainly internalized by specific receptor-mediated endocytosis. The average pH observed for internalized insulin was less than pH 6 within 10 min after addition of insulin. At 30 min, the average pH began to decrease to approximately pH 5, presumably because of fusion of endosomes with lysosomes

Evaluation of positive G sub Z tolerance following simulated weightlessness (bedrest)

The magnitude of physiologic changes which are known to occur in human subjects exposed to varying levels of + G sub Z acceleration following bed rest simulation of weightlessness was studied. Bed rest effects were documented by fluid and electrolyte balance studies, maximal exercise capability, 70 deg passive tilt and lower body negative pressure tests and the ability to endure randomly prescribed acceleration profiles of +2G sub Z, +3G sub Z, and +4G sub Z. Six healthy male volunteers were studied during two weeks of bed rest after adequate control observations, followed by two weeks of recovery, followed by a second two-week period of bed rest at which time an Air Force cutaway anti-G suit was used to determine its effectiveness as a countermeasure for observed cardiovascular changes during acceleration. Results showed uniform and significant changes in all measured parameters as a consequence of bed rest including a reduced ability to tolerate +G sub Z acceleration. The use of anti-G suits significantly improved subject tolerance to all G exposures and returned measured parameters such as heart rate and blood pressure towards or to pre-bed-rest (control) values in four of the six cases

The Effects of Endophyte-Infected KY 31 Tall Fescue Seed on Northern Bobwhite Reproduction

We assessed the impact of feeding an endophyte-free, endophyte-infected (Acremonium coenophialum), KY 31 tall fescue (Festuca arundinacea Schreb.) and a control diet on northern bobwhite (Colinus virginianus) reproduction. The birds consumed significantly more of the tall fescue diets compared to the control diet. There was no difference in female body weights at the end of the experiment. Male birds lost significantly more weight on the tall fescue diets than the control diet. The birds were in positive nutritional balances on all diets. There were no treatment effects on egg production, fertility, embryo mortality, hatch ability, or number of chicks per hen. Significantly more birds died eating endophyte-infected tall fescue seed compared to endophyte-free and control diets. These results indicate that tall fescue does not affect quail reproduction as indicated by previous authors. However, the endophyte does affect the weight gain of male birds and caused high mortality in these birds. We propose the alkaloids created by the endophyte caused a swelling of the cloaca which elicited a behavioral response in the birds causing them to become cannibalistic. These data support the idea that tall fescue does not provide quality nutritional habitat for northern bobwhite

Greedy for green: The motivations and hindrances of maintaining environmental sustainability in the commericial industry of cloud computing

This articleexploresthe potential for environmental sustainability in the rapidly growing industry of could computing. By understanding the cloud as a commercially driven endeavor, we unearth the motivations that drive the growth of the burgeoning industry. We go on to explain the impetus behind many of the data center innovations that cloud computing has brought about. We show that these innovations are why the cloud has been heralded as a form of sustainable computing. After exploring the technologies endorsed and innovated by cloud computing, the article proceeds todiscuss some of the potential downfalls inherent to propagating a commercially driven, yet environmentally sustainable industry. Lastly, the paper discusses the role that we, the consumer, play in shaping the environmental sustainability of the digital storage industry.Workshop: III Ethicomp latinoaméricaRed de Universidades con Carreras en Informática (RedUNCI

Gating-by-tilt of mechanosensitive membrane channels

We propose an alternative mechanism for the gating of biological membrane channels in response to membrane tension that involves a change in the slope of the membrane near the channel. Under biological membrane tensions we show that the energy difference between the closed (tilted) and open (untilted) states can far exceed kBT and is comparable to what is available under simple ilational gating. Recent experiments demonstrate that membrane leaflet asymmetries (spontaneous curvature) can strong effect the gating of some channels. Such a phenomenon would be more easy to explain under gating-by-tilt, given its novel intrinsic sensitivity to such asymmetry.Comment: 10 pages, 2 figure