Impact of Internet gambling on problem gambling among adolescents in Italy: Findings from a large-scale nationally representative survey

Aims: The primary aim of the present study was to understand the impact of online gambling on gambling problems in a large-scale nationally representative sample of Italian youth, and to identify and then further examine a subgroup of online gamblers who reported higher rates of gambling problems. Design: Data from the ESPAD®Italia2013 (European School Survey Project on Alcohol and Other Drugs) Study were used for analyses of adolescent Internet gambling. Setting: Self-administered questionnaires were completed by a representative sample of high school students, aged 15–19 years. Participants: A total of 14,778 adolescent students. Measurements: Respondents’ problem gambling severity; gambling behavior (participation in eight different gambling activities, the number of gambling occasions and the number of online gambling occasions, monthly gambling expenditure); Socio-demographics (e.g., family structure and financial status); and control variables were measured individually (i.e., use of the Internet for leisure activities and playing video games). Findings: Rates of problem gambling were five times higher among online gamblers than non-online gamblers. In addition, factors that increased the risk of becoming a problem online gambler included living with non-birth parents, having a higher perception of financial family status, being more involved with gambling, and the medium preferences of remote gamblers (e.g., Internet cafes, digital television, and video game console). Conclusions: The online gambling environment may pose significantly greater risk to vulnerable players. Family characteristics and contextual elements concerning youth Internet gambling (e.g., remote mediums) may play a key role in explaining problem online gambling among adolescents

Adapting the EMPIRIC Approach to Investigate Evolutionary Constraints in Influenza A Virus Surface Proteins

Controlling influenza A virus (IAV) infections remains a challenge largely due to the high replication and mutation rates of the virus. IAV is a negative-sense RNA virus with two main surface proteins — hemagglutinin (HA) and neuraminidase (NA). HA recognizes and binds sialic acid on host cell receptors to initiate virus entry. NA also recognizes sialic acid on host cell receptors but functions by cleaving sialic acid interactions to release progeny virus. Because both HA and NA interact with sialic acid on the host cell surface with opposing effects, their balance is essential for optimal viral infectivity. However, the evolutionary constraints that maintain HA and NA function, while conserving a functional balance, are not fully understood. I adapted the comprehensive and systematic mutational scanning technology, termed EMPIRIC (Exceedingly Meticulous and Parallel Investigation of Randomized Individual Codons), to investigate the local fitness landscape of regions of HA under standard conditions and under drug pressure. We observed that synonymous substitutions had a higher mean absolute fitness effect in the signal than a neighboring HA region used as a control. Folding ∆G calculations revealed a hairpin loop that appeared to be differentially enriched between human and swine IAV variants in sequences of circulating strains. However, the molecular mechanism resulting in the observed host species-specific constraints remains undefined. Studying the fitness landscape of the receptor binding site of HA revealed the high sensitivity of this region to mutation. However, modulating the levels of NA activity by mutation and by using the NA inhibitor oseltamivir enabled the identification of HA mutations with adaptive potential under selection pressure by oseltamivir. These results highlight the importance of the HA-NA functional balance virus replication and in the development of resistance to oseltamivir inhibitors. These studies provide improved understanding of IAV biology, and can inform the development of improved antiviral agents with reduced likelihood for resistance

Constrained Functional Time Series: Applications to the Italian Gas Market

Motivated by market dynamic modelling in the Italian Natural Gas Balancing Platform, we propose a model for analyzing time series of functions, subject to equality and inequality constraints at the two edges of the domain, respectively, such as daily demand and offer curves. Specifically, we provide the constrained functions with suitable pre-Hilbert structures, and introduce a useful isometric bijective map that associates each possible bounded and monotonic function to an unconstrained one. We introduce a functional-to-functional autoregressive model that is used to forecast future demand/offer functions, and estimate the model via the minimization of a penalized mean squared error of prediction, with a penalty term based on the Hilbert–Schmidt squared norm of autoregressive lagged operators. The approach is of general interest and could be generalized to any situation in which one has to deal with functions that are subject to the above constraints which evolve over time

AFM characterization of biomolecules in physiological environment by an advanced nanofabricated probe

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Changes in sleep pattern, sense of time and digital media use during COVID-19 lockdown in Italy

Italy is one of the major COVID-19 hotspots. To reduce the spread of the infections and the pressure on Italian healthcare systems, since March 10, 2020, Italy has been under a total lockdown, forcing people into home confinement. Here we present data from 1,310 people living in the Italian territory (Mage = 23.91 ± 3.60 years, 880 females, 501 workers, 809 university students), who completed an online survey from March 24 to March 28, 2020. In the survey, we asked participants to think about their use of digital media before going to bed, their sleep pattern and their subjective experience of time in the previous week (March 17–23, which was the second week of the lockdown) and up to the first week of February (February 3–10, before any restriction in any Italian area). During the lockdown, people increased the usage of digital media near bedtime, but this change did not affect sleep habits. Nevertheless, during home confinement, sleep timing markedly changed, with people going to bed and waking up later, and spending more time in bed, but, paradoxically, also reporting a lower sleep quality. The increase in sleep difficulties was stronger for people with a higher level of depression, anxiety and stress symptomatology, and associated with the feeling of elongation of time. Considering that the lockdown is likely to continue for weeks, research data are urgently needed to support decision making, to build public awareness and to provide timely and supportive psychosocial interventions

Low cycle fatigue predictions of a space thruster built with a new refractory high entropy alloy

Directional thrusters are designed to provide force for short time periods. • New generation of thrusters could be made using a High Entropy Alloy (HEA). • Predicting fatigue damage initiation for this kind of metallic structure subjected to cyclic loads is important. • Design for fatigue resistance relies on empirical with high financial costs. • Unified Mechanics Theory (UMT) will be used to predict fatigue damage initiation of a material system without having experimental fatigue dat

The multiplicative effect of combining alcohol with energy drinks on adolescent gambling

Purpose: There has been increased concern about the negative effects of adolescents consuming a combination of alcohol mixed with energy drinks (AmED). To date, few studies have focused on AmED use and gambling. The present study analyzed the multiplicative effect of AmED consumption, compared to alcohol alone, on the likelihood of at-risk or problem gambling during adolescence. Methods: Data from the ESPAD®Italia 2015 study, a cross-sectional survey conducted in a nationally representative sample of students (ages 15 to 19 years) were used to examine the association between self-reported AmED use (≥ 6 times, ≥ 10 times, and ≥ 20 times during the last month) and self-reported gambling severity. Multivariate models were used to calculate adjusted prevalence ratios to evaluate the association between alcohol use, AmED use, and gambling among a representative sample of adolescents who reported gambling in the last year and completed a gambling severity scale (n = 4495). Results: Among the 19% students classed as at-risk and problem gamblers, 43.9% were classed as AmED consumers, while 23.6% were classed as alcohol consumers (i.e. did not mix alcohol with energy drinks). In multivariate analyses that controlled for covariates, AmED consumers were three times more likely to be at-risk and problem gamblers (OR = 3.05) compared to non-consuming adolescents, while the effect became less pronounced with considering those who consumed alcohol without the addition of energy drinks (OR = 1.37). Conclusions: The present study clearly established that consuming AmED might pose a significantly greater risk of experiencing gambling-related problems among adolescents

Maternal Parenting Stress and Preschoolers’ Social-Emotional Competence and Behavioural Difficulties: A Variable- and Person-Centred Approach

Background: The present study examined the relations between maternal parenting stress and preschoolers’ psychosocial adjustment, using both a variable-centred and a person-centred approach. Methods: The study had three main purposes: (a) evaluating the associations of maternal parenting stress with children’s social-emotional competence and behavioural difficulties, as perceived by their mothers; (b) inquiring the existence of different children’s clusters based on their level of social-emotional competence and behavioural difficulties; (c) exploring differences in maternal parenting stress linked to cluster membership. Participants were 91 Italian mothers, aged from 22 to 47 years old (M = 35.14, SD = 5.80), having a preschool child from 3 to 6 years old (M = 4.6, SD = 0.80). Results: Overall, results showed that maternal parenting stress was negatively associated with children’s social-emotional competence, and positively associated with their behavioural difficulties. A cluster analysis allowed identifying four children’s profiles characterized by different levels of social-emotional competence and behavioural difficulties: Maladjusted, Troubled, Adjusted and Controversial. Conclusions: Results also evidenced differences among clusters in maternal parenting stress. Ultimately, this study suggests that interventions may consider reducing maternal parenting stress to promote children’s psychosocial adjustment