Opportunities for public private partnerships in IRPTN infrastructure

The Integrated Rapid Public Transport Network interventions in 13 cities in South Africa came about as a result of a forward thinking public transport strategy being presented by Government in 2007. Since then, costs of both implementation and operation have been shown to be higher than expected. The future budgeted capital funding required to roll out the proposed public transport investments are in excess of the level of grant funding likely to be available from the national fiscus. Third party funding options need to be actively investigated and developed to augment the capital investment allocations provided through the national fiscus. One such third party funding option is Public Private Partnerships in the development and operation of selected public transport infrastructure. This paper aims to reduce the mystique attached to Private Public Partnership (PPP) initiatives and seeks to link the use of PPP principles to the funding difficulties in IRPTNs. The paper concludes that PPP?s may well be a viable source of funding for selected elements or components of the IRPTNs. This will however require interventions at a National level in order to reduce the administrative burden on each of the 13 involved cities.Paper presented at the 34th Annual Southern African Transport Conference 6-9 July 2015 "Working Together to Deliver - Sakha Sonke", CSIR International Convention Centre, Pretoria, South Africa.The Minister of Transport, South AfricaTransportation Research Board of the US

A decade of seascape genetics: contributions to basic and applied marine connectivity

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Connectivity between marine reserves and exploited areas in the philopatric reef fish Chrysoblephus laticeps (Teleostei: Sparidae)

"No-take‟ Marine Protected Areas (MPAs) are successful in protecting populations of many exploited fish species, but it is often unclear whether networks of MPAs are adequately spaced to ensure connectivity among reserves, and whether spillover occurs into adjacent exploited areas. Such issues are particularly important in species with low dispersal potential, many of which exist as genetically distinct regional stocks.The roman, Chrysoblephus laticeps, is an overexploited, commercially important fishery species endemic to South Africa. Post-recruits display resident behavior and occupy small home ranges, making C. laticeps a suitable model species to investigate connectivity in marine teleosts with potentially low dispersal ability. We used multilocus data from two types of highly variable genetic markers (mitochondrial DNA control region and microsatellites) to clarify patterns of genetic connectivity and population structure in C. laticeps using samples from two MPAs and several moderately or severely exploited regions. Despite using analytical tools that are sensitive to detect even subtle genetic structure, we found that this species exists as a single, well-mixed stock throughout its core distribution. This finding lends supports to the status of MPAs as an adequate tool for managing overexploited marine teleosts. Even though adult dispersal out of MPAs is limited, the fact that the large adults in these reserves produce exponentially more offspring than their smaller counterparts in exploited areas makes MPAs a rich source of recruits. We nonetheless caution against concluding that the lack of structure identified in C. laticeps and several other southern African teleosts can be considered to be representative of marine teleosts in this region in general. Many such species are represented in more than one marine biogeographic province and may be comprised of regionally-adapted stocks that need to be managed individually

IRPTN vehicle financing options and considerations

The roll-out of comprehensive public transport networks in our major cities is requiring considerable up-front investment by government. The acquisition of the bus fleet presents the second largest component of capital expenditure by the implementing municipalities. Various fleet financing models have been adopted including the use of national grant funding and debt. This paper sets out to describe the various financing models that have been used to date and highlight their implications with respect to various criteria including cost, risk and complexity. The paper then moves on to discuss the concern of municipal treasuries surrounding the potential consolidation of the buses onto the books of the municipalities and evaluates the effectiveness of the move to finance the buses ?off-balance sheet?. The rationale for the National Department of Transport?s (?NDOT?) preference for the use of Export Credit Agency financing (?ECAs?) as the financing route of choice is also discussed. The paper then addresses the outcomes of an on- versus off-balance sheet financing structure, and the implications on both the financing model and the underlying institutional structure. An alternate model is then introduced which proposes a hybrid between the debt financing options used thus far in an attempt to mitigate the short comings present in the existing models.Paper presented at the 34th Annual Southern African Transport Conference 6-9 July 2015 "Working Together to Deliver - Sakha Sonke", CSIR International Convention Centre, Pretoria, South Africa.The Minister of Transport, South AfricaTransportation Research Board of the US

Identification of stomach contents from a Shepherd's beaked whale Tasmacetus shepherdi stranded on Tristan da Cunha, South Atlantic

Shepherd’s beaked whale Tasmacetus shepherdi is one of the most poorly known cetaceans, whose diet has created some speculation given that its dentition differs greatly from that of most other beaked whales that are primarily teuthophagous. The few stomachs examined previously have given seemingly conflicting dietary information. In this paper the stomach contents of a freshly stranded adult female on Tristan da Cunha have been examined through identification of trace elements and genetic analysis of soft parts. At least 13 cephalopod and 8 fish species were identified from beaks and otoliths respectively, but only undigested fish remains were present in the stomach and identified genetically as Beryx splendens. Reconstituted masses totaled 8809 g for cephalopods and 17,554 g for fish, with four species (Histioteuthis atlantica, Taningia danae, Ommastrephes bartrami and Pholidoteuthis ‘A’) comprising 78.6% of the cephalopods and one species (B. splendens) comprising 87.4% of the fish eaten. It is concluded that Tasmacetus may alternately exploit fish and cephalopods, depending on the time of day and access to seamount or continental slope areas.P.B.B. and M.J.S. acknowledge support from the National Research Foundation, South Africa, and S.v.d.H. thanks the Stellenbosch University Discretionary Fund for financial assistance.http://journals.cambridge.org/action/displayJournal?jid=mbi2015-09-30am201

Molecular ecology meets systematic conservation planning

Integrative and proactive conservation approaches are critical to the long-term persistence of biodiversity. Molecular data can provide important information on evolutionary processes necessary for conserving multiple levels of biodiversity (genes, populations, species, and ecosystems). However, molecular data are rarely used to guide spatial conservation decision-making. Here, we bridge the fields of molecular ecology (ME) and systematic conservation planning (SCP) (the ‘why’) to build a foundation for the inclusion of molecular data into spatial conservation planning tools (the ‘how’), and provide a practical guide for implementing this integrative approach for both conservation planners and molecular ecologists. The proposed framework enhances interdisciplinary capacity, which is crucial to achieving the ambitious global conservation goals envisioned for the next decade

Molecular ecology meets systematic conservation planning

Integrative and proactive conservation approaches are critical to the long-term persistence of biodiversity. Molecular data can provide important information on evolutionary processes necessary for conserving multiple levels of biodiversity (genes, populations, species, and ecosystems). However, molecular data are rarely used to guide spatial conservation decision-making. Here, we bridge the fields of molecular ecology (ME) and systematic conservation planning (SCP) (the ‘why’) to build a foundation for the inclusion of molecular data into spatial conservation planning tools (the ‘how’), and provide a practical guide for implementing this integrative approach for both conservation planners and molecular ecologists. The proposed framework enhances interdisciplinary capacity, which is crucial to achieving the ambitious global conservation goals envisioned for the next decade.Environment and Climate Change Canada (ECCC); Nature Conservancy of Canada; NERC Wallacea Programme Strategic Grant; an individual research contract by Fundação para a Ciência e Tecnologia; the National Research Foundation; a European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement.https://www.cell.com/trends/ecology-evolution/homeam2023BiochemistryGeneticsMicrobiology and Plant Patholog

Ubiquitous Presence of Fe(II) in Aquatic Colloids and Its Association with Organic Carbon

Despite being thermodynamically less stable, small ferrous colloids (60 nm to 3 μm in diameter) remain an important component of the biogeochemical cycle at the Earth’s surface, yet their composition and structure and the reasons for their persistence are still poorly understood. Here we use X-ray-based Fe L-edge and carbon K-edge spectromicroscopy to address the speciation and organic–mineral associations of ferrous, ferric, and Fe-poor particles collected from sampling sites in both marine and freshwater environments. We show that Fe(II)-rich phases are prevalent throughout different aquatic regimes yet exhibit a high degree of chemical heterogeneity. Furthermore, we show that Fe-rich particles show strong associations with organic carbon. The observed association of Fe(II) particles with carboxamide functional groups suggests a possible microbial role in the preservation of Fe(II). These finding have significant implications for the behavior of Fe(II) colloids in oxygenated waters, and their role in different aquatic biogeochemical processes

Reviewing evidence of marine ecosystem change off South Africa

Recent changes have been observed in South African marine ecosystems. The main pressures on these ecosystems are fishing, climate change, pollution, ocean acidification and mining. The best long-term datasets are for trends in fishing pressures but there are many gaps, especially for non-commercial species. Fishing pressures have varied over time, depending on the species being caught. Little information exists for trends in other anthropogenic pressures. Field observations of environmental variables are limited in time and space. Remotely sensed satellite data have improved spatial and temporal coverage but the time-series are still too short to distinguish long-term trends from interannual and decadal variability. There are indications of recent cooling on the West and South coasts and warming on the East Coast over a period of 20 - 30 years. Oxygen concentrations on the West Coast have decreased over this period. Observed changes in offshore marine communities include southward and eastward changes in species distributions, changes in abundance of species, and probable alterations in foodweb dynamics. Causes of observed changes are difficult to attribute. Full understanding of marine ecosystem change requires ongoing and effective data collection, management and archiving, and coordination in carrying out ecosystem research.DHE

Distribution and Phylogeny of EFL and EF-1α in Euglenozoa Suggest Ancestral Co-Occurrence Followed by Differential Loss

BACKGROUND: The eukaryotic elongation factor EF-1alpha (also known as EF1A) catalyzes aminoacyl-tRNA binding by the ribosome during translation. Homologs of this essential protein occur in all domains of life, and it was previously thought to be ubiquitous in eukaryotes. Recently, however, a number of eukaryotes were found to lack EF-1alpha and instead encode a related protein called EFL (for EF-Like). EFL-encoding organisms are scattered widely across the tree of eukaryotes, and all have close relatives that encode EF-1alpha. This intriguingly complex distribution has been attributed to multiple lateral transfers because EFL's near mutual exclusivity with EF-1alpha makes an extended period of co-occurrence seem unlikely. However, differential loss may play a role in EFL evolution, and this possibility has been less widely discussed. METHODOLOGY/PRINCIPAL FINDINGS: We have undertaken an EST- and PCR-based survey to determine the distribution of these two proteins in a previously under-sampled group, the Euglenozoa. EF-1alpha was found to be widespread and monophyletic, suggesting it is ancestral in this group. EFL was found in some species belonging to each of the three euglenozoan lineages, diplonemids, kinetoplastids, and euglenids. CONCLUSIONS/SIGNIFICANCE: Interestingly, the kinetoplastid EFL sequences are specifically related despite the fact that the lineages in which they are found are not sisters to one another, suggesting that EFL and EF-1alpha co-occurred in an early ancestor of kinetoplastids. This represents the strongest phylogenetic evidence to date that differential loss has contributed to the complex distribution of EFL and EF-1alpha