The interrelation between the electronic parameters of nitrogen atom and intramolecular interactions in ammonia derivatives

The electronic parameters and intramolecular interactions in the ground and transition states of inversion of the amines H₂NXHn (XHn=CH₃, NH₂, OH, F, SiH₃, PH₂, SH, Cl) were calculated using DFT (PBE96/def2-tzvpp) method. It was established that the electronacceptor properties of the XHn substituents has a prevailing influence on the change of the electronic parameters of nitrogen atom. Its increase leads to both a decrease of the charge on the nitrogen atom and an increase of the s-character and population of nitrogen lone pair (LP). All parameters under consideration correlate with the χ- and σᵢ-constants of the XHn substituents. The correlation coefficients increase when amines that contain X atoms only from one period are considered separately. It was found that the ρ values for amines containing X atoms from the second or third period are substantially different. The changes of the donor-acceptor interaction energies, s-character and LP population cannot be probable causes for different sensitivity of the electronic parameters of amines containing X atoms from different periods to the change of electron-acceptor properties of the XHn substituents. It was established that the mentioned parameters has only a subordinated influence in comparison with the influence of electron-acceptor ability of the XHn substituents. The negative charge on the nitrogen atom decreases with the increase of s-character and LP population and also with the decrease of energies of donor-acceptor interactions which lead to the withdrawal of electron density from the nitrogen atom. The s-character and LP population increase with the decrease of energies of donor-acceptor interactions which result in the reduction of electron density on the nitrogen atom. The total positive charges of the XHn groups and hydrogen atoms at the nitrogen atom decrease with increasing the electron-acceptor ability of the XHn substituents. The representation of the electron-acceptor properties of the substituents was shown to be more valid by using the χ-constants than by using the σᵢ-constants. The chlorine atom is a weak electron acceptor in comparison with an amino group

Diabetes insipidus and Guillain-Barré-like syndrome following CAR-T cell therapy: a case report

Background: Immune effector cell-associated neurotoxicity syndrome (ICANS) is a common adverse event of CD19-directed chimeric antigen receptor (CAR) T cell therapy. Other neurological adverse events, however, have not methodically been described and studied. Furthermore, safety data on CAR-T cell therapy in patients with central nervous system (CNS) lymphoma remain limited. Main body: We here report occurrence of a Guillain-Barré-like syndrome (GBS) and central diabetes insipidus (cDI) following tisagenlecleucel therapy for relapsed high-grade lymphoma with CNS involvement. Both complications were refractory to standard treatment of ICANS. Weakness of respiratory muscles required mechanical ventilation and tracheostomy while cDI was treated with desmopressin substitution for several weeks. Muscle-nerve biopsy and nerve conduction studies confirmed an axonal pattern of nerve damage. T cell-rich infiltrates and detection of the CAR transgene in muscle-nerve sections imply a direct or indirect role of CAR-T cell-mediated inflammation. In line with current treatment guidelines for GBS, intravenous immunoglobulin was administered and gradual but incomplete recovery was observed over the course of several months. Conclusions: This case report highlights the risk of rare but severe neurological adverse events, such as acute GBS or cDI, in patients treated with CAR-T cells. It further underlines the importance of appropriate patient surveillance and systematic reporting of rare complications to eventually improve treatment

Distant sequences determine 5′ end formation of cox3 transcripts in Arabidopsis thaliana ecotype C24

The genomic environments and the transcripts of the mitochondrial cox3 gene are investigated in three Arabidopsis thaliana ecotypes. While the proximate 5′ sequences up to nucleotide position −584, the coding regions and the 3′ flanking regions are identical in Columbia (Col), C24 and Landsberg erecta (Ler), genomic variation is detected in regions further upstream. In the mitochondrial DNA of Col, a 1790 bp fragment flanked by a nonanucleotide direct repeat is present beyond position −584 with respect to the ATG. While in Ler only part of this insertion is conserved, this sequence is completely absent in C24, except for a single copy of the nonanucleotide direct repeat. Northern hybridization reveals identical major transcripts in the three ecotypes, but identifies an additional abundant 60 nt larger mRNA species in C24. The extremities of the most abundant mRNA species are identical in the three ecotypes. In C24, an extra major 5′ end is abundant. This terminus and the other major 5′ ends are located in identical sequence regions. Inspection of Atcox3 transcripts in C24/Col hybrids revealed a female inheritance of the mRNA species with the extra 5′ terminus. Thus, a mitochondrially encoded factor determines the generation of an extra 5′ mRNA end

Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.

The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ∼95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/