17 research outputs found
Comparative study of in situ N2 rotational Raman spectroscopy methods for probing energy thermalisation processes during spin-exchange optical pumping
Spin-exchange optical pumping (SEOP) has been widely used to produce enhancements in nuclear spin polarisation for hyperpolarised noble gases. However, some key fundamental physical processes underlying SEOP remain poorly understood, particularly in regards to how pump laser energy absorbed during SEOP is thermalised, distributed and dissipated. This study uses in situ ultra-low frequency Raman spectroscopy to probe rotational temperatures of nitrogen buffer gas during optical pumping under conditions of high resonant laser flux and binary Xe/N2 gas mixtures. We compare two methods of collecting the Raman scattering signal from the SEOP cell: a conventional orthogonal arrangement combining intrinsic spatial filtering with the utilisation of the internal baffles of the Raman spectrometer, eliminating probe laser light and Rayleigh scattering, versus a new in-line modular design that uses ultra-narrowband notch filters to remove such unwanted contributions. We report a ~23-fold improvement in detection sensitivity using the in-line module, which leads to faster data acquisition and more accurate real-time monitoring of energy transport processes during optical pumping. The utility of this approach is demonstrated via measurements of the local internal gas temperature (which can greatly exceed the externally measured temperature) as a function of incident laser power and position within the cell
Hyperpolarized Xe MR imaging of alveolar gas uptake in humans.
BACKGROUND: One of the central physiological functions of the lungs is to transfer inhaled gases from the alveoli to pulmonary capillary blood. However, current measures of alveolar gas uptake provide only global information and thus lack the sensitivity and specificity needed to account for regional variations in gas exchange. METHODS AND PRINCIPAL FINDINGS: Here we exploit the solubility, high magnetic resonance (MR) signal intensity, and large chemical shift of hyperpolarized (HP) (129)Xe to probe the regional uptake of alveolar gases by directly imaging HP (129)Xe dissolved in the gas exchange tissues and pulmonary capillary blood of human subjects. The resulting single breath-hold, three-dimensional MR images are optimized using millisecond repetition times and high flip angle radio-frequency pulses, because the dissolved HP (129)Xe magnetization is rapidly replenished by diffusive exchange with alveolar (129)Xe. The dissolved HP (129)Xe MR images display significant, directional heterogeneity, with increased signal intensity observed from the gravity-dependent portions of the lungs. CONCLUSIONS: The features observed in dissolved-phase (129)Xe MR images are consistent with gravity-dependent lung deformation, which produces increased ventilation, reduced alveolar size (i.e., higher surface-to-volume ratios), higher tissue densities, and increased perfusion in the dependent portions of the lungs. Thus, these results suggest that dissolved HP (129)Xe imaging reports on pulmonary function at a fundamental level
HP <sup>129</sup>Xe MR signal intensity in human lungs.
<p>(<b>A</b>) NMR spectrum obtained using a hard, 7Β° RF pulse. The gaseous HP <sup>129</sup>Xe signal is used as a 0 ppm reference. (<b>B</b>) Spectrum from a selective, 7Β° pulse centered at 218 ppm. The 218-ppm peak arises from <sup>129</sup>Xe dissolved in the red blood cells (RBC), and the 197-ppm arises from <sup>129</sup>Xe in the blood plasma and semi-solid parenchymal tissues. (<b>C</b>) Dissolved HP <sup>129</sup>Xe signal dynamics during single breath-hold radial imaging. Data points represent the magnitude of k-zero from each radial view weighted by the initial HP <sup>129</sup>Xe polarization (P). Even using a relatively large flip angle of Ξ±βΌ17Β° and a rapid TR of 4.2 ms, substantial dissolved signal is still observed at the end of the breath-hold period due to rapid, diffusive replenishment of dissolved <sup>129</sup>Xe magnetization.</p