Population dynamics of the last leopard population of eastern Indochina in the context of improved law enforcement

Poaching, a major threat to wildlife worldwide, is pushing species toward extinction. To reduce poaching pressure and combat biodiversity loss, improved law-enforcement efforts reportedly are required; the effectiveness of which can be determined through rigorous monitoring of wildlife populations, particularly of endangered large carnivores. In the Eastern Plains Landscape of Cambodia, law-enforcement efforts increased to counter the severe threats from illegal activities; however, it is unknown if these strategies are benefiting the population of the Critically Endangered Indochinese leopard (Panthera pardus delacouri), the last large felid population of eastern Indochina. We used open population spatial capture-recapture models to estimate density, survival, per-capita recruitment, and population growth rates of leopard using data from 7 camera-trap surveys conducted over 11 years (2009–2019). We found that the population (a) declined by over 82 % (from 1.5 to 0.3 leopard/100 km2), (b) had low survival probability (0.58) and low recruitment rates (males: 0.04, females: 0.24), and (c) is expected to continue declining. An additional survey in 2021 failed to detect leopard, suggesting the species now is functionally extinct, if not fully extirpated, from the landscape. Over the study period, there was a drastic increase in human activity, with a 20-fold increase in detection frequencies of humans and a 1000-fold increase in lethal-trap encounter rate. The rise in anthropogenic pressures, particularly snaring, appeared to be the primary reason for the leopard decline, indicating the last decade of management interventions was insufficient to conserve the species, which now appears to be extirpated in all of Cambodia, Laos and Vietnam. This has implications for leopard conservation in the wider region, notably that efforts should now focus on populations within the two remaining strongholds. Our results suggest that increases in law-enforcement efforts alone are unlikely to protect wildlife in eastern Indochina; thus, additional strategies are needed to address the region's snaring crisis, including legislative reforms, community engagement, and programs that reduce demand for wildlife meat and products. Long-term studies of remaining Indochinese leopard populations coupled with timely and effective conservation actions are needed to avoid the complete demise of this subspecies

Historical biogeography of the leopard (Panthera pardus) and its extinct Eurasian populations

Background: Resolving the historical biogeography of the leopard (Panthera pardus) is a complex issue, because patterns inferred from fossils and from molecular data lack congruence. Fossil evidence supports an African origin, and suggests that leopards were already present in Eurasia during the Early Pleistocene. Analysis of DNA sequences however, suggests a more recent, Middle Pleistocene shared ancestry of Asian and African leopards. These contrasting patterns led researchers to propose a two-stage hypothesis of leopard dispersal out of Africa: an initial Early Pleistocene colonisation of Asia and a subsequent replacement by a second colonisation wave during the Middle Pleistocene. The status of Late Pleistocene European leopards within this scenario is unclear: were these populations remnants of the first dispersal, or do the last surviving European leopards share more recent ancestry with their African counterparts? Results: In this study, we generate and analyse mitogenome sequences from historical samples that span the entire modern leopard distribution, as well as from Late Pleistocene remains. We find a deep bifurcation between African and Eurasian mitochondrial lineages (~ 710 Ka), with the European ancient samples as sister to all Asian lineages (~ 483 Ka). The modern and historical mainland Asian lineages share a relatively recent common ancestor (~ 122 Ka), and we find one Javan sample nested within these. Conclusions: The phylogenetic placement of the ancient European leopard as sister group to Asian leopards suggests that these populations originate from the same out-of-Africa dispersal which founded the Asian lineages. The coalescence time found for the mitochondrial lineages aligns well with the earliest undisputed fossils in Eurasia, and thus encourages a re-evaluation of the identification of the much older putative leopard fossils from the region. The relatively recent ancestry of all mainland Asian leopard lineages suggests that these populations underwent a severe population bottleneck during the Pleistocene. Finally, although only based on a single sample, the unexpected phylogenetic placement of the Javan leopard could be interpreted as evidence for exchange of mitochondrial lineages between Java and mainland Asia, calling for further investigation into the evolutionary history of this subspecies

Scale dependence of felid predation risk: identifying predictors of livestock kills by tiger and leopard in Bhutan

Context: Livestock predation by tiger and leopard in Bhutan is a major threat to the conservation of these felids. Conflict mitigation planning would benefit from an improved understanding of the spatial pattern of livestock kills by the two predators. Objectives: We aimed to identify the landscape features that predict livestock kills by tiger and leopard throughout Bhutan. Our goals were to: (1) identify the predictors that have the largest influence in determining livestock kills, (2) assess the influence of scale across the different predictors evaluated and identify the scale at which each was most important. Methods: We used livestock kills obtained from compensation records of tiger (n = 326) and leopard (n = 377) across Bhutan between 2003 and 2012 to run predation risk models with MaxEnt algorithm, using a multi-scale modeling approach (1, 2, 4, 8 and 16 km). Results: Human-presence (density of settlements and roads) and land-cover (percentage of tree cover and meadow patches) were the main variables contributing to livestock kills by both species. Livestock kills were likely driven by a trade-off between livestock density and predator ecology, and the balance of this trade-off varied with scale. Risk maps revealed different hotspots for tiger and leopard kills, and analysis showed both species preferentially killed equids over other livestock types. Conclusions: Our results highlight the importance of evaluating scale when investigating the spatial attributes of livestock kills by tiger and leopard. Our findings provide guidance for reducing conflict between humans and large felids throughout the country

Big cats in borderlands: challenges and implications for transboundary conservation of Asian leopards

Large carnivores have extensive spatial requirements, which often result in ranges that span geopolitical borders. Consequently, management of transboundary populations is subject to different political jurisdictions, often with high heterogeneity in conservation challenges. In continental Asia, there are four endangered leopard subspecies with transboundary populations spanning 23 countries: the Persian, Indochinese, Arabian, and Amur leopards. We reviewed the status of these subspecies and examined their conservation challenges and opportunities. Amur and Indochinese leopards had the majority (58-100%) of their remaining range in borderlands, whereas Persian and Arabian leopards had a quarter (23-26%) of their remaining ranges in borderlands. Overall, in 18 of 23 countries the majority of the remaining leopard range was in borderlands, thus in most countries their conservation is dependent on transboundary collaborations. However, we found only two transboundary initiatives for Asian leopards. Overall, we highlighted three key transboundary landscapes in regions which are of high importance for the survival of these subspecies. Recent listing of leopard in the Bonn Convention is an encouraging step forward, but more international collaboration is needed to save these subspecies. Our paper provides a spatial framework on which range countries and international agencies can establish transboundary cooperation for conserving endangered leopards in Asia

An adaptable but threatened big cat: density, diet and prey selection of the Indochinese leopard (Panthera pardus delacouri) in eastern Cambodia

We studied the Indochinese leopard (Panthera pardus delacouri) in eastern Cambodia, in one of the few potentially remaining viable populations in Southeast Asia. The aims were to determine the: (i) current leopard density in Srepok Wildlife Sanctuary (SWS) and (ii) diet, prey selection and predation impact of leopard in SWS. The density, estimated using spatially explicit capture–recapture models, was 1.0 leopard/100km2, 72% lower than an estimate from 2009 at the same site, and one of the lowest densities ever reported in Asia. Dietary analysis of 73 DNA confirmed scats showed leopard consumed 13 prey species, although ungulates comprised 87% of the biomass consumed (BC). The overall main prey (42% BC) was banteng (Bos javanicus), making this the only known leopard population whose main prey had adult weight greater than 500 kg. Consumption of wild pig (Sus scrofa) was also one of the highest ever reported (22% BC), indicating leopard consistently predated on ungulates with some of the largest adult weights in SWS. There were important differences in diet and prey selection between sexes, as males consumed mostly banteng (62% BC) in proportion to availability, but few muntjac (Muntiacus vaginalis; 7% BC), whereas females selectively consumed muntjac (56% BC) and avoided banteng (less than 1% BC). Predation impact was low (0.5–3.2% of populations) for the three ungulate species consumed. We conclude that the Indochinese leopard is an important apex predator in SWS, but this unique population is declining at an alarming rate and will soon be eradicated unless effective protection is provided

Density and occupancy of leopard cats across different forest types in Cambodia

The leopard cat (Prionailurus bengalensis) is the most common wild felid in Southeast Asia, yet little is known about the factors that affect their population density and occupancy in natural habitats. Although leopard cats are highly adaptable and reportedly can attain high densities in human-modified habitats, it is not clear which natural habitat is optimal for the species. Also, this felid has been preyed upon by large carnivores in Southeast Asia, yet the intra-guild effects of large carnivore presence on leopard cats are almost unknown. To shed light on these fundamental questions, we used data from camera trap surveys for felids to determine the leopard cat densities in three different forest types within Cambodia: continuous evergreen, mosaic dominated by evergreen (hereafter evergreen mosaic), and mosaic dominated by open dry deciduous forests (hereafter DDF mosaic). We also conducted occupancy analyses to evaluate the interactions of the leopard cats with three large carnivores: leopards (Panthera pardus), dholes (Cuon alpinus), and domestic dogs (Canis familiaris). The estimated density (individuals/100 km2 ± SE) was highest in the continuous evergreen (27.83 ± 7.68), followed by evergreen mosaic (22.06 ± 5.35) and DDF mosaic (13.53 ± 3.23). Densities in all three forest types were relatively high compared to previous studies. Domestic dogs were detected on all 3 sites, and leopards and dholes had sufficient records on only one site each. The occupancy probability of leopard cats was not affected by the presence or absence of any large carnivore, indicating that large carnivores and leopard cats occurred independently of each other. Our findings support the claim that leopard cats are habitat generalists, but we show that evergreen forest is the optimum natural habitat for this species in the region. The DDF mosaic appears to sustain lower densities of leopard cats, probably due to the harsh dry season and wildfires that led to reduced prey base, although this generalist felid was still able to occupy DDF in relatively moderate numbers. Overall, the adaptability of leopard cats to various forest types, and lack of negative interaction with large carnivores, helps to explain why this species is the most common and widespread felid in Southeast Asia

Leopard (Panthera pardus) status, distribution, and the research efforts across its range

The leopard’s (Panthera pardus) broad geographic range, remarkable adaptability, and secretive nature have contributed to a misconception that this species might not be severely threatened across its range. We find that not only are several subspecies and regional populations critically endangered but also the overall range loss is greater than the average for terrestrial large carnivores. To assess the leopard’s status, we compile 6,000 records at 2,500 locations from over 1,300 sources on its historic (post 1750) and current distribution. We map the species across Africa and Asia, delineating areas where the species is confirmed present, is possibly present, is possibly extinct or is almost certainly extinct. The leopard now occupies 25–37% of its historic range, but this obscures important differences between subspecies. Of the nine recognized subspecies, three (P. p. pardus, fusca, and saxicolor) account for 97% of the leopard’s extant range while another three (P. p. orientalis, nimr, and japonensis) have each lost as much as 98% of their historic range. Isolation, small patch sizes, and few remaining patches further threaten the six subspecies that each have less than 100,000 km2 of extant range. Approximately 17% of extant leopard range is protected, although some endangered subspecies have far less. We found that while leopard research was increasing, research effort was primarily on the subspecies with the most remaining range whereas subspecies that are most in need of urgent attention were neglected