Supplementary data for article: Kop, T.; Bjelaković, M.; Milić, D. Synthesis and Properties of Bis(Pyrrolidino)Fullerenes Bridged by a Flexible Alkyl-Tether. Tetrahedron 2015, 71 (29), 4801–4809. https://doi.org/10.1016/j.tet.2015.05.038

Supplementary material for: [https://doi.org/10.1016/j.tet.2015.05.038]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1729

An improved HPLC-DAD method for simultaneously measuring phenolics in the leaves of Tilia platyphyllos and Ailanthus altissima

Phenolic compounds are one of the most important groups of secondary metabolites in plants, with various physiological functions. Their diverse chemical structure and susceptibility to auto-oxidation, and their ability to act as both antioxidants and prooxidants in the presence of metal ions, are some of the main reasons why it is difficult to measure phenolic groups in plant tissues accurately. We present an optimized extraction and hydrolysis procedure which preserves the original chemical structure of phenolics. The presented HPLC method was improved to enable the simultaneous separation and quantification of 39 compounds from different phenolic subclasses (benzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanones, flavanols, isoflavones, anthocyanidins; aglycones and glycosides). Recovery after extraction and complete hydrolysis of glycosides was more than 95% and 84%, respectively. The method was applied to the analysis and comparison of phenolic profiles in the leaves of two species, Ailanthus altissima (Mill.) Swingle and Tilia platyphyllos Scop. from an urban forest park and busy traffic area. The presence of cyanidin glycosides in A. altissima leaves was reported for the first time. Results indicated higher accumulation of phenolics, with two hydroxyl groups in the ortho- position, than flavonoids, with a monohydroxy substitution in the leaves of both species from a busy traffic area

Changes in Crude Protein Content with Advancing Maturity in Lucerne

The main determinants of the quality of lucerne forage are digestibility and protein content (Julier et al., 2001) as well as crude fibre content. In the early vegetative phases, the crude protein content of the leaves and stems is the highest and crude fibre content the lowest (Katic et al., 2003). The aim of this study was to determine the rate of change in crude protein levels at different stages of growth and development

Efficacy and safety of lidocaine with clonidine for intraoral local anaesthesia in patients with diabetes mellitus type 2

Uvod Pacijenti sa dijabetes melitusom (DM), zbog pratećih makrovaskularnih i mikrovaskularnih komplikacija osnovnog oboljenja, koje zahvataju i periferne nerve (dijabetična neuropatija), predstavljaju pacijente rizika za primenu regionalne anestezije. Pokazano je u eksperimentalnim uslovima da lokalni anestetici, u klinički primenljivim koncentracijama, izazivaju veća oštećenja na nervima izmenjenim dijabetičnom neuropatijom, što se u kliničkim uslovima manifestuje promenjenim parametrima regionalne anestezije. Promene koje DM izaziva na krvnim sudovima i nervima su zapažene i u tkivima i organima orofacijalne regije. Za sada nema podataka o parametrima i bezbednosti primene intraoralne lokalne anestezije u uslovima DM. Ciljevi Ovo istraživanje je imalo za ciljeve da ispita i uporedi efikasnost i bezbednost intraoralne lokalne anestezije postignute primenom 2% lidokaina sa klonidinom (15 mcg / ml) (LC) i 2% lidokaina sa epinefrinom (1:80 000) (LE) kod zdravih i ispitanika sa DM tip 2, praćenjem kliničkih parametara anestezije zubne pulpe i mekih tkiva, protoka krvi kroz zubnu pulpu, parametara postoperativne analgezije, učestalosti lokalnih neželjenih efekata, kao i parametara kardiovaskularne funkcije u uslovima sa i bez oralnohirurške intervencije. Materijal i metod Ova prospektivna, dvostruko slepa, randomizovana, kontrolisana klinička studija je izvedena u dva dela. U jednom delu studije učestvovalo je 36 zdravih (ASA I) i 36 ispitanika sa DM tip 2 (ASA II i ASA III), koji su u ukrštenom dizajnu istraživanja bez oralnohirurške intervencije, u razmaku od 14 dana dobili ispitivane anestetičke rastvore (LC i LE) za maksilarnu infiltracionu anesteziju, pri čemu su ispitivani protok krvi kroz zubnu pulpu maksilarnih centralnih sekutića, kao i parametri anestezije zubne pulpe i kardiovaskularne funkcije. U drugom delu studije učestvovalo je 106 zdravih (ASA I) i 127 ispitanika sa DM tip 2 (ASA II i ASA III), koji su prema zdravstvenom statusu, tehnici anestezije (infiltraciona, sprovodna) i vrsti anestetičkog rastvora (LC, LE) podeljeni u 8 grupa...Introduction Patients with diabetes mellitus (DM) type 2 are at increased risk for performance of regional anaesthesia, due to macrovascular and microvascular complications of DM, including changes in small blood vessels of peripheral nerves (diabetic neuropathy). Results of experimental studies show that local anesthetics, even in clinically applicable concentrations, cause more pronounced damage to the nerves in the presence of DM, with clinically observable changes in parameters of regional anaesthesia. Neuropathic and microangiopathic changes caused by DM are also observed in the oral cavity tissues of DM patients. To date, there are no available data on efficacy and safety of intraoral local anaestehsia in patients with DM. Aims The aims of this doctoral dissertation were to test and compare efficacy and safety of intraoral local anaesthesia obtained with 2% lidocaine with clonidine (15 mcg / ml) (LC) and 2% lidocaine with epinephrine (1:80 000) (LE) in healthy and DM type 2 participants, by assessing the parameters of pulpal and soft tissues anaesthesia, pulpal blood flow, parameters of postoperative analgesia, presence of local side effects, as well as parameters of cardiovascular functions in the settings with and without oral surgical procedure. Material and method This prospective, double blind, randomized, controlled clinical study was performed in two parts. One part of the study included 36 healthy (ASA I) i 36 DM tip 2 participants (ASA II i ASA III), who received tested anaesthetic solutions (LC i LE) for maxillary infiltration anestehesia, in cross over-manner with washout period of 14 days, in the setting without oral surgical procedure. Tested parameters were: pulpal blood flow, parameters of pulpal anaesthesia and cardiovascular functions. Following part of the study involved 106 healthy (ASA I) i 127 DM tip 2 patients (ASA II i ASA III), divided into 8 groups according to health status, anaesthesia technique (infiltration, block) and tested anesthetic solution (LC, LE). In this study population the tested parameters were: soft tissue anaesthesia, postoperative analgesia, local side effects and cardiovascular parameters after infiltration and block application of either LC or LE, for extraction of single-rooted teeth in upper and lower jaw. Pulpal blood flow of maxillary central incisors was measured by laser Dopler flowmetry prior to, as well as 5, 10, 15, 30, 45 and 60 minutes after maxillary infiltration anaesthesia..

Supplementary data for article: Kop, T. J.; Dordevic, J.; Bjelakovic, M. S.; Milić, D. Fullerene Bisadduct Regioisomers Containing an Asymmetric Diamide Tether. Tetrahedron 2017, 73 (50), 7073–7078. https://doi.org/10.1016/j.tet.2017.10.069

Supplementary material for: [https://doi.org/10.1016/j.tet.2017.10.069]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2565

Stanje tvrdog i mekog periimplantnog tkiva u estetskoj regiji gornje vilice posle prezervacije alveolarnog grebena beta-trikalcijum fosfatom sa kolagenom tip I - studija sa jednogodišnjim periodom praćenja

Background/Aim. Alveolar ridge dimensional alterations following tooth extraction in the anterior maxilla often result in an inadequate bone volume for a correct implant placement. In order to obtain optimal bone volume various bone graft substitutes have become commercially available and widely used for socket grafting. The aim of this study was to examine and compare long-term clinical outcomes of dental implant therapy in the maxillary esthetic zone, after socket grafting with beta-tricalcium phosphate (TCP) combined with collagen type I, either with or without barrier membrane and flap surgery, after a 12-month follow-up. Methods. Twenty healthy patients were allocated to either C group (beta-TCP and type I collagen without mucoperiosteal flap coverage) or C+M group (beta-TCP and type I collagen barrier membrane with mucoperiosteal flap coverage). Following clinical parameters were assessed: implant stability (evaluated by a resonance frequency analysis - RFA), periimplant soft tissue stability (sulcus bleeding index - SBI, Mombelli sulcus bleeding index - MBI, periimplant sulcus depth, keratinized gingiva width, gingival level) and marginal bone level at the retroalveolar radiograms. Results. Within C+M group, RFA values significantly increased 12 weeks after implant installation compared to primary RFA values. Comparison between investigated groups showed a significantly reduced keratinized gingiva width in the C+M group compared to the C group after 3, 6, 9 and 12 months. Comparison between groups revealed significantly lower gingival level values in the C+M group at 9th and 12th month when compared to the C group. Conclusion. Implant treatment in the anterior maxilla could be effective when using a 9 months alveolar ridge preservation healing with combined treatment with beta-tricalcium phosphate and type I collagen, with regard to the peri-implant soft and hard tissue stability.Uvod/Cilj. Posle ekstrakcije zuba, dimenzionalne promene alveolarnog grebena u estetskoj regiji gornje vilice za posledicu često imaju nedovoljnu količinu kosti za ugradnju zubnih implanata. U vezi sa tim, primenjuju se različiti koštani zamenici sa ciljem očuvanja dimenzija alveolarnog grebena posle ekstrakcije zuba. Cilj rada bio je da se, posle prezervacije alveolarnog grebena beta-trikalcijum fosfatom (TCP) sa kolagenom tip 1, sa barijernom membranom i mukoperiostalnim režnjem i bez nje, ispitaju i uporede klinički ishodi zarastanja posle ugradnje zubnih implanata u estetskoj regiji gornje vilice, tokom jednogodišnjeg perioda praćenja. Metode. Dvadeset zdravih bolesnika podeljeno je u dve grupe: C (beta TCP/kolagen tip 1 bez barijerne membrane i mukoperiostalnog režnja) i C+M (beta TCP/kolagen tip 1 sa barijernom membranom i mukoperiostalnim režnjem). Praćeni su uobičajeni klinički parametri ishoda terapije: implantna stabilnost (analiza rezonantne frekvence), stanje mekih tkiva (indeks krvarenja, plak indeks, širina pripojne mukoze, recesija gingive) i nivo periimplantnog koštanog tkiva na retroalveolarnom radiogramu. Rezultati. U C+M grupi, implantna stabilnost posle 12 nedelja bila je značajno veća u odnosu na primarnu stabilnost. U C+M grupi, širina keratinizovane gingive bila je značajno manja posle 3, 6, 9 i 12 meseci u odnosu na C grupu. Recesija gingive bila je značajno veća u C+M grupi u odnosu na C grupu posle 9 i 12 meseci. Zaključak. Razmatrajući stabilnost mekog i tvrdog periimplantnog tkiva, terapija zubnim implantima može biti uspešna prilikom ugradnje u estetskoj regiji gornje vilice

Supplementary data for article: Bjelaković, M. S.; Kop, T. J.; Dordević, J.; Milić, D. R. Fulleropeptide Esters as Potential Self-Assembled Antioxidants. Beilstein Journal of Nanotechnology 2015, 6 (1), 1065–1071. https://doi.org/10.3762/bjnano.6.107

Supporting information for: [https://doi.org/10.3762/bjnano.6.107]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1695

Supplementary data for the article: Bjelaković, M.; Kop, T.; Maslak, V.; Milić, D. Synthesis and Characterization of Highly Ordered Self-Assembled Bioactive Fulleropeptides. Journal of Materials Science 2016, 51 (2), 739–747. https://doi.org/10.1007/s10853-015-9396-z

Supplementary material for: [https://doi.org/10.1007/s10853-015-9396-z]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1999

Histohemijska, imunohistohemijska i morfometrijska analiza promena na srčanom mišiću kod heroinskih zavisnika

In order to clarify the mechanism of heroin addicts death, besides changes in the lungs, which were most frequently studied, changes in the heart muscle were also observed. The weakening of the contractility of the myocardium and the binding of the connective tissue in the heart muscle are considered to be the basis for the development of possible pathogenetic mechanisms of dying. In order to define micro-morphological changes on the myocardium, histochemical, immunohistochemical and morphometric analysis determines the qualitative characteristics of cardiomyocytes and connective tissue, as well as the number of inflammatory cells in the myocardium (leucocytes, monocytes and macrophages, Tlymphocytes and mastocytes) of 42 cadavers whose death is in relation to the heroin abuse, and 10 cadavers from the control group. The quantification of these parameters was performed by measuring the thickness of the myocardiocyte and determining the percentage of connective tissue representation, and counting specific cell types in 30 visual fields in each of the 6 myocardial samples taken from the defined sites. The mean values were analyzed statistically. The connective tissue was statistically significantly present in the heroin addicts myocard in relation to the control group (p = 0.001). Cardiomyocytes were statistically significantly thicker in heroine addicts for p = 0.005. The number of leukocyte was significantly lower in the left ventricle compared to the right ventricle and the intraventricular septum (p = 0.02) for heroin addicts. Statistically significant differences between the sexes and between the groups defined by the length of heroin use were not recorded for the analyzed parameters. Cardiomyocyte hypertrophy, presence of connective tissue, and the presence of certain immune response cells indicate that prolonged use of the herion over time can lead to dysfunction of the heart muscle and heartbeat system, which may represent the cause of the death of heroin addicts

The Effect of a Compulsory Curriculum on Ethical Attitudes of Medical Students

The purpose of the present study was to assess the influence of a compulsory curriculum on first-year medical students. The ethical attitude study was performed at the School of Medicine, University of Rijeka, Croatia. The samples consisted of 171 medical students (68 males and 103 females) interviewed at the beginning of the first year of studies. Some of them, namely 143 (56 males and 87 females) were interviewed again at the end of the same academic year. Data were analysed by applying factor analysis under principal component model and varimax criterion as the rotation model. The results clearly show that the current compulsory curriculum without formal ethical education has a limited influence on first-year medical students. That points to the obvious necessity to implement the medical ethics in the course of medical education