Development of a rapid screen for the endodermal differentiation potential of human pluripotent stem cell lines.

A challenge facing the human pluripotent stem cell (hPSC) field is the variability observed in differentiation potential of hPSCs. Variability can lead to time consuming and costly optimisation to yield the cell type of interest. This is especially relevant for the differentiation of hPSCs towards the endodermal lineages. Endodermal cells have the potential to yield promising new knowledge and therapies for diseases affecting multiple organ systems, including lung, thymus, intestine, pancreas and liver, as well as applications in regenerative medicine and toxicology. Providing a means to rapidly, cheaply and efficiently assess the differentiation potential of multiple hPSCs is of great interest. To this end, we have developed a rapid small molecule based screen to assess the endodermal potential (EP) of hPSCs, based solely on definitive endoderm (DE) morphology. This drastically reduces the cost and time to identify lines suitable for use in deriving endodermal lineages. We demonstrate the efficacy of this screen using 10 different hPSCs, including 4 human embryonic stem cell lines (hESCs) and 6 human induced pluripotent stem cell lines (hiPSCs). The screen clearly revealed lines amenable to endodermal differentiation, and only lines that passed our morphological assessment were capable of further differentiation to hepatocyte like cells (HLCs)

A Review of management of Kamala w.s.r to Hepatitis through Shamanaushadi

Kamala which is caused by aggrevated Pitta, may occur with or without Pandu Roga (Swatantra and Paratantra Vyadhi). In this Vyadhi, the hunger and appetite is diminished, mala gets discoloured (Peeta, Haridra, Harithadi) In classical texts of Ayurveda, we come across the explanation of Rakta Pradoshaja vikaras. Kamala is a Pittaja Nanatmaja and Rakta Pradoshaja Vyadhi. Most of the people have become used to spicy fast-food day by day. This has taken human being far away from the nature. Nowadays alcohol consumption is also increasing day by day. General symptoms of Hepatitis include, intense abdominal pain, yellowing of eyes and skin, nausea, extreme tiredness and dark urine.  This article aims at discussing the about the Kamala (Hepatitis). This paper also discusses the Ayurvedic medicines like Triphala, Guduchi, Nimba, Vasa, Katuki. that can be helpful in curing this infection and their properties are discussed as well

A case study on Kitibha Kusta (Psoriasis) with Samshodana Chikitsa

All the skin diseases in Ayurveda have been discussed under the broad heading of “Kustha”. In general Twak Vikaras are mainly affecting due to altered lifestyle, lack of physical exercise, poor hygiene, mental stress and improper food habits. Kitibha Kushta is one of the Kshudra Kushta. Here Tridosha, Rasa, Rakta, Mamsa, Ambu are affected. Kapha-Vata is the Dosha predominant in the Kitibha Kustha and Rakta Dusti is the main cause for all Twak Vikaras. Vamana and Virechana is the Shodhana Karma for Twak Vikara. After Shodhana, Shamana Chikitsa plays major role for Alpadosha Nirharana. This paper highlights a case study of Kitibha Kushta as a Raktavahasrotodusti Vikara treated with the Ayurvedic principles in particular Shodhana Chikitsa

A scientific review on Dadrukushta with special reference to Dermatophytosis and its management through Ayurveda

Skin is the largest organ of the body and skin diseases has a high prevalence throughout the world and fungal infection of skin is one of them. In the high populated countries like India and China the incidence of skin diseases is considerably increased in recent years due to predisposing factors like warm, humid climate, crowded living conditions, hygiene and poor nutrition. In Ayurveda all the skin related diseases are explained under main heading of Kushta and skin fungal infections termed as Dadrukushta (Dermatophytosis). Ayurveda has a versatile approach towards skin disorders depending on the requirement of the patient. The present article is aimed at understanding the disease Dadrukushta with special reference to Dermatophytosis and its management through Ayurveda

An open labeled clinical trail on the effect of Vrikshamla in Sthaulya (obesity) w.s.r to hyperlipidaemia

Obesity is one of the leading cause for more than 53 diseases and in India around 30 million peoples are suffering with Sthaulya (obesity), individual habits with stressful life, wrong dietary habits and sedentary life is the main cause of disease and no satisfactory treatment is available in conventional system. Hence there is need explore alternative system of Indian medicine like Ayurveda for the benefits of sufferers Ayurveda dealt this disease as Sthaulya (obesity) under Medoroga (Adipose tissue disorder) and many treatment modalities have been explained number of drugs in Brahatrayi (Charaka, Sushruta & Vagbhat) were cited Bhavamishra was the author of Bhavaprakash Nighantu (Lexicon) which is one of the Laghutrayis (Sharanghadara, Madhavanidhana & Bhavaprakash) text is highlighted with Karmaoushadi (action based drug) such as Amradi Phala Varga explained Vrikshamla considered as drug index of Ayurveda, thus present study was designed to screen the drug with properties of Kapha Medohara (ability to reduce Kapha & Fat), Medorogahara (reduces Adipose tissue), Sthaulyanashaka (Anti obesity) and Karshyakara (ability to make lean) from Bhavaprakasha Nighantu in Ayurveda

GESTURE BASED TOUCHPAD SECURITY SYSTEM WITH DESIGN OF TOUCH SCREEN CONTROLLER

The purpose of the project is to present a new approach on the design of security systems by using a touch sensitive device. Security is a permanent concern in a variety of environments ranging from physical access restriction in home and industrial settings to information security in digital systems. Numeric passwords, fingerprint recognition, and many other techniques have been extensively implemented in the past but they present certain drawbacks. The proposed technique makes use of a touch device to recognize symbols as passwords and takes time into account to add a new dimension and prevent password theft. We implemented a prototype that demonstrates the capabilities of the proposed security approach by showing one direct application in physical access restriction systems

Ayurvedic understanding and management of Trigeminal Neuralgia w.s.r. to Anantavaata - A Case Study

Trigeminal Neuralgia is one of the painful disorders involving fifth cranial nerve, causes severe unilateral pain over regions supplied by the branches of trigeminal nerve associated with tempero mandibular joint stiffness. Trigeminal neuralgia nearly can be correlated to Anantavaata, one of the 11 types of Shirorogas explained by Acharya Sushruta and is Vaatapradhaana Tridoshaja Vyadhi. Present study is conducted to know the efficacy of ayurvedic line of management in TN. A female patient of age 46 years, diagnosed with Trigeminal Neuralgia was treated with allopathy medications. But did not get satisfactory results. Hence patient approached our hospital and got Vaatahara and Brihmana line of management involving Nasya with Karpasasthyaadi Taila for 7 days, Shiropichu and Karnapoorana with Ksheerabala Taila, Gandoosha with Murchita Tila Taila and oral medications. Patient’s complaints were assessed before and after treatment with VAS pain scale and verbal descriptor scale for TMJ stiffness After Follow up patient had got relieved with the complaints and got satisfactory results with ayurvedic line of management

Pregnancy with pancytopenia: an observational study

Background: Pancytopenia is the reduction in all three major cellular elements of blood; hence it is the simultaneous presence of anaemia, leukopenia and thrombocytopenia. Pancytopenia is associated with many maternal and foetal complications during pregnancy like maternal sepsis, postpartum haemorrhage, pre-eclampsia and preterm labour, IUGR and intrauterine foetal demise.Methods: The study was conducted at BLDE (Deemed to be) University, Shri B. M. Patil Medical College Hospital and Research Centre. It’s an observational study done from November 2019-April 2020. The participants enrolled in the study were subjected for further clinical and laboratory evaluation and followed for feto-maternal outcome.Results: The incidence of pancytopenia with pregnancy in this study was 3% and all the patients were vegetarian by diet along with vitamin B12 and folic acid deficiency. They were associated with different maternal and foetal complications.Conclusions: Proper dietary counselling and well-balanced dietary plans even with plant originate food can prevent the micronutrients deficiency and avoid the deleterious consequences like pancytopenia

Simultaneous estimation of Etodolac and Paracetamol in bulk drug dosage form by RP-HPLC

A simple, specific, rapid, economical and accurate reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Etodolac and Paracetamol in bulk drug and tablet dosage form. Sepration was achieved by capcell pack C-18 column having 250 mm4.6 mm i.d. in isocratic mode, with mobile phase containing 25 nM potassium dihydrogen phosphate buffer(adjusted to pH 6.1 using ortho phosphoric acid) : methanol (40:60). The flow rate was 1.0 ml/min and effluents were monitored at 235 nm. The retention time of Etodolac and Paracetamol were 3.19 min and 6.08 min respectively. The linearity for Etodolac and Paracetamol were in the range of 10-100 ?g/ml. The recoveries of Etodolac and Paracetamol were found in the range of 99.27-99.78 % and 99.74-99.88 % respectively. The proposed method was validated as per ICH and USP guidelines and successfully applied to the estimation of Etodolac and Paracetamol in bulk drug and tablet dosage form