Evaluación Radiográfica de la Frecuencia y Distribución de los Nódulos Pulpares

Fil: Labarta, AB. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia; Argentina.Fil: Mancipe Leguizamón, S. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia; Argentina.Fil: Pineda Martínez, CJ. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia; Argentina.Fil: Sierra, LG. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia; Argentina.Objetivos: Estimar la prevalencia de nódulos pulpares y agujas cálcicas en pacientes atendidos en la Cátedra de Endodoncia, Facultad de Odontología, Universidad de Buenos Aires (CEFOUBA). Se analizaron radiografías de 1866 piezas dentarias, con ápices maduros, de pacientes de ambos géneros, mayores de 15 años, tratados en CEFOUBA, en el período comprendido entre junio-noviembre de 2018. Género, edad, grupo dentario, condición del diente, presencia de patología sistémica, y ubicación anatómica, fueron las variables registradas. Se calcularon intervalos de confianza 95%, método score de Wilson. Se utilizó test z para diferencias de proporciones

Glycerylphytate-crosslinked microgels as MSC-delivery platform improves cell survival and function

Mesenchymal stem cells (MSCs) exhibit multiple beneficial properties, most notably potentimmunomodulation via their secretory factors that render them as an attractive cell source for cell therapy. However, theirclinical application remains limited due to their low survival and persistence in vivo, highlighting the need for new cell-carriers1. Chitosan is a widely applied biomaterial, but its poor solubility has constrained its use as a cell carrier2. Weexamined the fabrication of chitosan-based MSCs-microcarriers using a water-soluble chitosan lactate (ChLA) derivativeand in situ crosslinking in a microfluidic device using tripolyphosphate (TPP) and glycerylphytate (G1Phy) as ioniccrosslinkers. G1Phy is a powerful antioxidant,3 whose crosslinking ability has been evinced4. Our microgel synthesisallows for cell encapsulation, and G1Phy provided beneficial features to microgels in terms of MSCs survival, persistenceand secretome modulation

Microfluidics generation of chitosan microgels containing glycerylphytate crosslinker for in situ human mesenchymal stem cells encapsulation

The authors thank the Spanish Ministry of Science and Innovation (project, MAT2017-84277-R) and U.S. National Institutes of Health (R01 AR062368) for financial support. B. Vázquez-Lasa and J. San Román are members of the SusPlast platform (Interdisciplinary Platform for Sustain-able Plastics towards a Circular Economy) from the Spanish National Research Council (CSIC). Ana Mora-Boza was supported by “La Caixa” Foundation (ID 100010434, scholarship code LCF/BQ/ES16/11570018) and CIBER-BBN (Health Institute Carlos III) Travel gran

Electrospun Nanofibers Loaded with <i>Plantago major</i> L. Extract for Potential Use in Cutaneous Wound Healing

Plantago major L. is a plant available worldwide that has been traditionally used for several medical applications due to its wound healing, anti-inflammatory, and antimicrobial properties. This work aimed to develop and evaluate a nanostructured PCL electrospun dressing with P. major extract encapsulated in nanofibers for applications in wound healing. The extract from leaves was obtained by extraction in a mixture of water:ethanol = 1:1. The freeze-dried extract presented a minimum inhibitory concentration (MIC) for Staphylococcus Aureus susceptible and resistant to methicillin of 5.3 mg/mL, a high antioxidant capacity, but a low content of total flavonoids. Electrospun mats without defects were successfully produced using two P. major extract concentrations based on the MIC value. The extract incorporation in PCL nanofibers was confirmed using FTIR and contact angle measurements. The PCL/P. major extract was evaluated using DSC and TGA demonstrating that the incorporation of the extract decreases the thermal stability of the mats as well as the degree of crystallinity of PCL-based fibers. The P. major extract incorporation on electrospun mats produced a significant swelling degree (more than 400%) and increased the capacity of adsorbing wound exudates and moisture, important characteristics for skin healing. The extract-controlled release evaluated using in vitro study in PBS (pH, 7.4) shows that the P. major extract delivery from the mats occurs in the first 24 h, demonstrating their potential capacity to be used in wound healing

Combined effect of the essential oil from Chenopodium ambrosioides and antileishmanial drugs on promastigotes of Leishmania amazonensis Efeito combinado do óleo de essência de Chenopodium ambrosioides e drogas anti-leishmaniose nos promastigotas de Leishmania amazonensis

To date, there are no vaccines against Leishmania, and chemotherapy remains the mainstay for the control of leishmaniasis. The drugs of choice used for leishmaniasis therapy are significantly toxic, expensive and with a growing frequency of refractory infections. Because of these limitations, a combination therapy is the better hope. This work demonstrates that the essential oil from Chenopodium ambrosioides shows a synergic activity after incubation in conjunction with pentamidine against promastigotes of Leishmania amazonensis. However, an indifferent effect has been found for combinations of meglumine antimoniate or amphotericin B and the essential oil.<br>Até hoje não temos vacina contra a Leishmania e a quimioterapia é a indicação para o controle desta doença. Os remédios que hoje utilizamos são tóxicos e muito caros e além disso o resultado não é sempre o desejado. Por isso, uma terapia de combinação é a melhor opção. Este trabalho mostra que o óleo de essência de C. ambrosioides tem atividade sinérgica junto com a pentamidina sobre os promastigotas de L. amazonensis, diferente do resultado da combinação de antimônio de meglumine e anfotericina B e o óleo de essência