A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant

Pentyl gallate (PG) is a gallic acid n-alkyl ester with a promising antiherpetic topical activity. No method for PG quantification is described in the literature. Therefore, the objective of this investigation is to develop and validate a simple method for determination of PG by High performance liquid chromatography (HPLC) considering its future application in therapy. A nanoemulsion was proposed as PG delivery system due to its low aqueous solubility that limits its delivery through the skin. Chromatographic separation was achieved on a C18 column with 50:49:1 (%, v/v) methanol:water:trifluoroacetic acid (TFA) as mobile phase at a flow rate of 1.2 mL/min; detection at 275 nm. The method was validated for linearity, precision, accuracy, robustness and specificity. The degradant detected under alkaline condition was structurally characterized by time-of-flight mass spectrometry (TOF-MS). All results indicate the method is adequate for PG analysis.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Preparation of a Nanoemulsion with Carapa guianensis

Andiroba (Carapa guianensis) seeds are the source of an oil with a wide range of biological activities and ethnopharmacological uses. However, few studies have devoted attention to innovative formulations, including nanoemulsions. The present study aimed to obtain a colloidal system with the andiroba oil using a low-energy and organic-solvent-free method. Moreover, the preliminary residual larvicidal activity of the nanoemulsion against Aedes aegypti was evaluated. Oleic and palmitic acids were the major fatty acids, in addition to the phytosterol β-sitosterol and limonoids (tetranortriterpenoids). The required hydrophile-lipophile was around 11.0 and the optimal nanoemulsion was obtained using polysorbate 85. The particle size distribution suggested the presence of small droplets (mean diameter around 150 nm) and low polydispersity index (around 0.150). The effect of temperature on particle size distribution revealed that no major droplet size increase occurred. The preliminary residual larvicidal assay suggested that the mortality increased as a function of time. The present study allowed achievement of a potential bioactive oil in water nanoemulsion that may be a promising controlled release system. Moreover, the ecofriendly approach involved in the preparation associated with the great bioactive potential of C. guianensis makes this nanoemulsion very promising for valorization of this Amazon raw material

Preparation of a nanoemulsion with Carapa guianensis Aublet (Meliaceae) oil by a low-energy/solvent-free method and evaluation of its preliminary residual larvicidal activity.

Andiroba (Carapa guianensis) seeds are the source of an oil with a wide range of biological activities and ethnopharmacological uses. However, few studies have devoted attention to innovative formulations, including nanoemulsions.The present study aimed to obtain a colloidal system with the andiroba oil using a low-energy and organic-solvent-free method. Moreover, the preliminary residual larvicidal activity of the nanoemulsion against Aedes aegypti was evaluated.Made available in DSpace on 2019-10-14T18:16:13Z (GMT). No. of bitstreams: 1 CPAFAP2017PreparationofananoemulsionwithCarapa.pdf: 2242910 bytes, checksum: ec164ab7c444265a84ee22d13657afe8 (MD5) Previous issue date: 2017bitstream/item/202848/1/CPAF-AP-2017-Preparation-of-a-nanoemulsion-with-Carapa.pd

Multicentric study on the diagnosis of Fabry's disease using angiokeratoma biopsy registries

Univ São Paulo, Inst Crianca, Fac Med, Genet Unit, São Paulo, SP, BrazilHosp Nove de Julho, Serv Reumatol, São Paulo, SP, BrazilUniv São Paulo, Serv Genet, Fac Med Ribeirao Preto, São Paulo, SP, BrazilUniversidade Federal de São Paulo, Dept Psicol, São Paulo, SP, BrazilHosp Santa Casa de Misericordia São Paulo, Serv Patol, São Paulo, SP, BrazilUniv São Paulo, Fac Med, Dept Dermatol, São Paulo, SP, BrazilUniversidade Federal de São Paulo, Dept Patol, São Paulo, SP, BrazilUnidade Genet ICr, Genet Unit, BR-05403000 São Paulo, SP, BrazilUniversidade Federal de São Paulo, Dept Psicol, São Paulo, SP, BrazilUniversidade Federal de São Paulo, Dept Patol, São Paulo, SP, BrazilWeb of Scienc

Preparation and crystallographic analysis of gliclazide polymorphs

Since the introduction of gliclazide in the pharmaceutical industry, a large number of research groups have been engaged in various investigations aiming to enhance its biomedical application. But, very limited efforts have been made to study polymorphism of gliclazide. Therefore, this study focuses on solvent-induced polymorphism of gliclazide and its characterization by thermal methods. Three polymorphs namely, Form-I, II and III and an amorphous powder were produced from different solvents and solvent mixtures. Crystals were analyzed using infrared spectroscopy, differential scanning calorimetry, X-ray powder diffraction and single crystal x-ray diffraction. Polymorph Form-I is found to exist in centro-symmetric triclinic P-1 space group and has endothermic peak at 162.93°. Form-II has endothermic peak from 171.2° to 172.35° and exists in centro-symmetric monoclinic P2 1 /a space group while Form-III has endothermic peak from 168.93° to 169.86° and exists in centro-symmetric monoclinic P2 1 /n space group. The equilibrium solubility values of Form-I, II, III and the amorphous form were 0.4825±0.025, 0.2341±0.042, 0.2581±0.038 and 0.5213±0.072 mg/ml, respectively. The Form-I has relatively higher solubility and similar to that of amorphous gliclazide. Form-II and Form-III are relatively most stable and least soluble. However, there was no remarkable difference in their aqueous solubility under the conditions in which study was conducted