Molecular cloning and transcriptional regulation of ompT , a ToxR-repressed gene in Vibrio cholerae

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72189/1/j.1365-2958.2000.01699.x.pd

Spinodal breakdown and magnetic properties of Fe-Cr-Co alloys

28.00; Translated from Czech. (Kovove Mater. 1989 v. 27(5) p. 634-646)Available from British Library Document Supply Centre- DSC:9023.19(VR-Trans--4490)T / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

Dendritic growth of crystals in Aurix alloy

Translated from Czech. (Kovove Mater. 1988 v. 26(3) p. 276-293)Available from British Library Document Supply Centre- DSC:9023.19(VR-Trans--3934)T / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

Cassava Metabolomics and Starch Quality

Abstract Cassava plays an important role as a staple food for more than 800 million people in the world due to its ability to maintain relatively high productivity even in nutrient‐depleted soils. Even though cassava has been the focus of several breeding programs and has become a strong focus of research in the last few years, relatively little is currently known about its metabolism and metabolic composition in different tissues. In this article, the absolute content of sugars, organic acids, amino acids, phosphorylated intermediates, minerals, starch, carotenoids, chlorophylls, tocopherols, and total protein as well as starch quality is described based on multiple analytical techniques, with protocols specifically adjusted for material from different cassava tissues. Moreover, quantification of secondary metabolites relative to internal standards is presented using both non‐targeted and targeted metabolomics approaches. The protocols have also been adjusted to apply to freeze‐dried material in order to allow processing of field harvest samples that typically will require long‐distance transport. © 2019 The Authors. Basic Protocol 1: Metabolic profiling by gas chromatography–mass spectrometry (GC‐MS) Support Protocol 1: Preparation of freeze‐dried cassava material Support Protocol 2: Preparation of standard compound mixtures for absolute quantification of metabolites by GC‐MS Support Protocol 3: Preparation of retention‐time standard mixture Basic Protocol 2: Determination of organic acids and phosphorylated intermediates by ion chromatography–mass spectrometry (IC‐MS) Support Protocol 4: Preparation of standards and recovery experimental procedure Basic Protocol 3: Determination of soluble sugars, starch, and free amino acids Alternate Protocol: Determination of soluble sugars and starch Basic Protocol 4: Determination of anions Basic Protocol 5: Determination of elements Basic Protocol 6: Determination of total protein Basic Protocol 7: Determination of non‐targeted and targeted secondary metabolites Basic Protocol 8: Determination of carotenoids, chlorophylls, and tocopherol Basic Protocol 9: Determination of starch qualit

Dissection and engineering of the <i>Escherichia coli </i>formate hydrogenlyase complex

AbstractThe Escherichia coli formate hydrogenlyase (FHL) complex is produced under fermentative conditions and couples formate oxidation to hydrogen production. In this work, the architecture of FHL has been probed by analysing affinity-tagged complexes from various genetic backgrounds. In a successful attempt to stabilize the complex, a strain encoding a fusion between FdhF and HycB has been engineered and characterised. Finally, site-directed mutagenesis of the hycG gene was performed, which is predicted to encode a hydrogenase subunit important for regulating sensitivity to oxygen. This work helps to define the core components of FHL and provides solutions to improving the stability of the enzyme