Apparent digestibility of insect protein meals for rainbow trout

Insect meals are considered to be promising future ingredients for aquaculture feeds. In past feeding trials in rainbow trout, insect meals were included in diets only on the basis of their nutrients content and energy density without taking into account their biological availability due to the lack of their digestible values. Apparent digestibility (ADC) provides good indication of the bioavailability of nutrients and energy thus providing rational basis for the correct inclusion of feedstuffs. The aim of this research was to assess, in an in vivo trial on rainbow trout, the ADC of five full fat insect meals: one Tenebrio molitor (TM), two Hermetia illucens obtained through two different process (HI1 and HI2), one Musca domestica (MD), and one Alphitobius diaperinus (AD). Fish were fed a high-quality reference diet (R) and test diets obtained mixing the R diet with each of the test ingredients at a ratio of 70:30. Diets contained 1% celite as inert marker. Fish were fed to visual satiety twice a day and faecal samples collected using a continuous automatic device. Faeces were freeze dried and frozen (-20 \ub0C) until analyses. The ADC of dry matter, crude protein and ether extract of each insect meal diet were calculated. ADC for dry matter varied between 70.07 (HI1) and 80.85 (TM). ADC for protein was above 84% in all treatments and resulted the highest in MD, TM and AD treatments. Ether extract apparent digestibility significantly differed among diets with the highest value reported for TM treatment. All treatments reported values higher than 96%. Observed differences could be due to the insect species and meal treatment but in general, tested insect meals were highly digestible for rainbow trout. The results from this research could be useful to optimize the diet formulation

Effect of dietary supplementation with insect fats on growth performance, digestive efficiency and health of rabbits

Background: The present work aimed at evaluating the effect of the dietary replacement of soybean oil (S) by two types of insect fats extracted from black soldier fly larvae (H, Hermetia illucens L.) and yellow mealworm larvae (T, Tenebrio molitor L.) on growth performance, nutrient digestibility, blood parameters, intestinal morphology and health of growing rabbits. Methods: At weaning, 200 crossbred rabbits (36 days old) were allotted to five dietary treatments (40 rabbits/group): a control diet (C) containing 1.5% of soybean oil and four experimental diets where soybean oil was partially (50%) or totally (100%) substituted by H (H50 and H100) or T (T50 and T100) fats. Total tract digestibility was evaluated on 12 rabbits per treatment. The growth trial lasted 41 d and, at slaughtering (78 days old), blood samples were collected from 15 rabbits per treatment, morphometric analyses were performed on duodenum, jejunum and ileum mucosa, and samples of liver, spleen and kidney were submitted to histological evaluation. Results: No difference was observed between the control and the experimental groups fed insect fats in terms of performance, morbidity, mortality and blood variables. The addition of H and T fats did not influence apparent digestibility coefficients of dry matter, protein, ether extract, fibre fractions and gross energy. Gut morphometric indices and organ histopathology were not affected by dietary inclusion of H and T fats. Conclusions: H and T fats are suitable sources of lipid in rabbit diets to replace soybean oil without any detrimental effect on growth performance, apparent digestibility, gut mucosa traits and health

Evaluation of carcass and meat traits of Muscovy duck fed with black soldier fly partially defatted meal

The aim of this study was to evaluate the carcass characteristics and breast meat quality in Muscovy duck (Cairina moschata domestica) fed different inclusion levels of a partially defatted black soldier fly larva (BSF) meal. A total of 256 Muscovy ducklings (average live weight, LW: 71.32\ub12.70 g) were reared from day 3 to day 48 and randomly allotted in 32 pens (8 replicates/treatment). Four different diets were formulated with increasing substitution level of corn gluten meal with BSF larva meal (0, 3, 6 and 9%; BSF0, BSF3, BSF6 and BSF9, respectively) and divided in 3 feeding phases: starter (1-14 days), grower (14-35 days) and finisher (35-48 days). At day 48, 2 animals/replicate were slaughtered and dissected to determine their carcass yields. The weights of spleen, bursa of Fabricius, liver, heart and abdominal fat were recorded. Breast and thigh muscles were then excised from 16 ducks/treatment and weighted. Ultimate pH (pHu) and L*, a*, b* colour values were then measured on breast muscle. The collected data were tested by means of oneway ANOVA evaluating the effect of dietary BSF inclusion level by polynomial contrasts. Significance was declared at P<0.05. The inclusion of BSF did not affect final LW (2,515.68\ub192.42 g on average). Hot and cold carcass weights showed a quadratic response (P<0.05) to increasing BSF larva meal, with a minimum corresponding to BSF6; however, refrigeration losses were not affected by treatments. Weight of spleen, bursa of Fabricius, liver and heart did not differ among treatments. The weight of abdominal fat showed a quadratic response to increasing BSF meal with a minimum corresponding to BSF6 group (P<0.05). Breast and thigh yields, pHu and L*, a*, b* colour values did not differ among groups. With the exception of BSF6, the inclusion of BSF meal did not affect meat traits and carcass characteristics, confirming the potential use of BSF meal in Muscovy duck diets

Insect-based feed in aquaculture: a consumer attitudes study

The aquaculture industry is currently faced with the major challenge of finding alternative protein sources for feeding aquatic species. The goal is to mitigate the environmental impact of conventional feed production in order to satisfy the demand of consumers for sustainable and environmentally friendly food. Fishmeal and fish oil have been the predominant substrates used in the fish farming industry to date, but insects are now emerging as promising feed substitutes. However, the feeding of insects to fish continues to be perceived as unconventional by consumers, although only few studies have actually explored European consumers' attitudes towards animal food products fed with insects. This study aimed to fill this gap by investigating consumer behaviors towards the consumption of fish fillets obtained from fish fed an insect-based feed. The overall goal was to understand the interconnection between sociodemographic variables, namely levels of knowledge, food neophobia, and food consumption sustainability, and attitudes towards feed quality, climate change, shopping sustainability, and the sustainability of insect-based feed. To this end, an online survey was conducted on 303 Italian consumers aged 18–78 years (52.4% men). The analysis of the dataset was conducted by modelling the independent categorical variables with their attitudes towards the four topics studied by Multiple Linear Regression, after having established their effects using the Pearson Chi-Square test and one-way ANOVA. Our results demonstrate that sociodemographic variables, such as gender and age, are strongly correlated with attitudes towards climate change, while diet is associated with attitudes towards shopping sustainability. The measured level of food consumption sustainability correlates with both attitudes. At the same time, a high level of knowledge correlates with a strong attitude towards the sustainability of insect-based feed. Overall, we conclude that providing specific groups of consumers with meaningful information related to the use of insect-based feed in aquaculture will increase the likelihood of their accepting this innovation. Our study also offers insights that can help identify categories of consumers who could be more interested in choosing products from insect-fed animal

Evaluation of extra-virgin olive oils shelf life using an electronic tongue-chemometric approach

Physicochemical quality parameters, olfactory and gustatoryretronasal positive sensations of extra-virgin olive oils vary during storage leading to a decrease in the overall quality. Olive oil quality decline may prevent the compliance of olive oil quality with labeling and significantly reduce shelf life, resulting in important economic losses and negatively condition the consumer confidence. The feasibility of applying an electronic tongue to assess olive oils usual commercial light storage conditions and storage time was evaluated and compared with the discrimination potential of physicochemical or positive olfactory/gustatory sensorial parameters. Linear discriminant models, based on subsets of 58 electronic tongue sensor signals, selected by the meta-heuristic simulated annealing variable selection algorithm, allowed the correct classification of olive oils according to the light exposition conditions and/or storage time (sensitivities and specificities for leave-one-out cross-validation: 8296 %). The predictive performance of the E-tongue approach was further evaluated using an external independent dataset selected using the KennardStone algorithm and, in general, better classification rates (sensitivities and specificities for external dataset: 67100 %) were obtained compared to those achieved using physicochemical or sensorial data. So, the work carried out is a proof-of-principle that the proposed electrochemical device could be a practical and versatile tool for, in a single and fast electrochemical assay, successfully discriminate olive oils with different storage times and/or exposed to different light conditions.The authors acknowledge the financial support from the strategic funding of UID/BIO/04469/2013 unit, from Project POCI-01-0145-FEDER-006984—Associate Laboratory LSRELCM funded by FEDER funds through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI)—and by national funds through FCT—Fundação para a Ciência e a Tecnologia and under the strategic funding of UID/BIO/04469/2013 unit. Nuno Rodrigues thanks FCT, POPH-QREN and FSE for the Ph.D. Grant (SFRH/BD/104038/2014).info:eu-repo/semantics/publishedVersio