МЕТОДОЛОГІЯ ВПРОВАДЖЕННЯ СИСТЕМИ ГРОМАДСЬКОГО ЗДОРОВ’Я НА РІВНІ ОБ’ЄДНАНИХ ТЕРИТОРІАЛЬНИХ ГРОМАД

Purpose: to develop methodological approaches to the implementation of the public health system at the level of united territorial communities. Materials and Methods. The bibliographic method and the method of structural-logical analysis were used during the research. The system approach constituted the methodological basis of the study. The current legislation of Ukraine and data from scientific publications were used in the research. Results. The generalized tasks and functions of public health at the level of united territorial community are promoting preservation and strengthening of the health of the community population. Areas of this activity are: development of a comprehensive public health program, epidemiological surveillance of health risks, establishing the level of individual health of community members, rehabilitation for people with disabilities and people with chronic diseases, the formation of health-preserving profile of community settlements, communication and social mobilization in the interests of health, prevention of infectious and non-infectious diseases. An algorithm for the implementation and development of the public health system at the level of united territorial communities has been developed, which consists of 12 stages with the study and analysis of the public health situation, development and implementation of a comprehensive inter-sectoral public health program, monitoring and evaluation its implementation, annual reporting to the community on the implementation of a comprehensive inter-sectoral program for the development of the public health system in the community and its adjustment. A public health professional can work in the community in the following positions: public health professional or environmental and health professional. Conclusions. The use of the proposed algorithm will create an effective public health system at the community level to preserve and strengthen health of the community.Мета: розробити методичні підходи до впровадження системи громадського здоров’я на рівні об’єднаних територіальних громад. Матеріали і методи. У ході виконання дослідження використано бібліографічний метод та метод структурно-логічного аналізу. Методичною основою дослідження став системний підхід. Під час проведення дослідження використано чинні законодавчі акти України та дані наукових публікацій. Результати. Узагальненими завданнями та функціями громадського здоров’я на рівні об’єднаних територіальних громад є сприяння збереженню та зміцненню здоров’я населення громади. Напрямками даної діяльності є розробка комплексної програми громадського здоров’я, епіднагляд за ризиками для здоров’я, встановлення рівня індивідуального здоров’я членів громади, відновне лікування для осіб з інвалідністю та осіб з хронічними хворобами, формування здоров’язбережувального профілю населених пунктів громади, комунікація та соціальна мобілізація в інтересах здоров’я, профілактика інфекційних та неінфекційних хвороб. Розроблено алгоритм впровадження та розвитку системи громадського здоров’я на рівні об’єднаних територіальних громад, який складається з 12 етапів з проведенням вивчення та аналізу ситуації щодо громадського здоров’я, розробки та впровадження комплексної міжсекторальної програми громадського здоров’я, моніторингу та оцінки її виконання, щорічного звітування перед громадою про хід виконання комплексної міжсекторальної програми розвитку системи громадського здоров’я в громаді та її корегування. Спеціаліст громадського здоров’я може працювати в громаді на таких посадах: фахівець з громадського здоров’я або фахівець з довкілля та здоров’я. Висновки. Використання запропонованого алгоритму дозволить створити на рівні громад ефективну систему громадського здоров’я зі збереження та зміцнення здоров’я населення громади

Veillonella rogosae sp. nov., an anaerobic, Gram-negative coccus isolated from dental plaque

Strains of a novel anaerobic, Gram-negative coccus were isolated from the supra-gingival plaque of children. Independent strains from each of six subjects were shown, at a phenotypic level and based on 16S rRNA gene sequencing, to be members of the genus Veillonella. Analysis revealed that the six strains shared 99.7 % similarity in their 16S rRNA gene sequences and 99.0 % similarity in their rpoB gene sequences. The six novel strains formed a distinct group and could be clearly separated from recognized species of the genus Veillonella of human or animal origin. The novel strains exhibited 98 and 91 % similarity to partial 16S rRNA and rpoB gene sequences of Veillonella parvula ATCC 10790T, the most closely related member of the genus. The six novel strains could be differentiated from recognized species of the genus Veillonella based on partial 16S rRNA and rpoB gene sequencing. The six novel strains are thus considered to represent a single novel species of the genus Veillonella, for which the name Veillonella rogosae sp. nov. is proposed. The type strain is CF100T (=CCUG 54233T=DSM 18960T)

Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963

Actinomyces naeslundii is an important early colonizer in the oral biofilm and consists of three genospecies (1, 2 and WVA 963) which cannot be readily differentiated using conventional phenotypic testing or on the basis of 16S rRNA gene sequencing. We have investigated a representative collection of type and reference strains and clinical and oral isolates (n=115) and determined the partial gene sequences of six housekeeping genes (atpA, rpoB, pgi, metG, gltA and gyrA). These sequences identified the three genospecies and differentiated them from Actinomyces viscosus isolated from rodents. The partial sequences of atpA and metG gave best separation of the three genospecies. A. naeslundii genospecies 1 and 2 formed two distinct clusters, well separated from both genospecies WVA 963 and A. viscosus. Analysis of the same genes in other oral Actinomyces species (Actinomyces gerencseriae, A. israelii, A. meyeri, A. odontolyticus and A. georgiae) indicated that, when sequence data were obtained, these species each exhibited <90 % similarity with the A. naeslundii genospecies. Based on these data, we propose the name Actinomyces oris sp. nov. (type strain ATCC 27044T =CCUG 34288T) for A. naeslundii genospecies 2 and Actinomyces johnsonii sp. nov. (type strain ATCC 49338T =CCUG 34287T) for A. naeslundii genospecies WVA 963. A. naeslundii genospecies 1 should remain as A. naeslundii sensu stricto, with the type strain ATCC 12104T =NCTC 10301T =CCUG 2238T

Clinical and anatomic features of cleft lip and palate

Cleft lip and palate is the most common congenital anomaly, being the second in the structure of antenatal pathologies. It takes the first place among the anom alies of dento-facial development. The work presents the analysis of medical records of 2961 children with cleft lip and palate from their birth till 16 years old. In the structure of clinical forms more severe states prevail, these being cleft palates and com bined clefts, making up 72.8% .Врождённая расщелина нёба является наиболее часто встречающимся пороком развития человека и занимает в структуре антенатальной патологии второе место. Среди аномалий развития челюстно-лицевой области эта патология занимает первое место. В работе представлен анализ данных медицинской документации 2961 ребенка от рождения до 16 лет с врожденной расщелиной губы и неба. У детей с ВРГН в структуре клинических форм преобладали более тяжелые формы — врожденные расщелины нёба и комбинированные расщелины, что составило в сумме 72,8%

Citric acid wastewater as electron donor for biological sulfate reduction

Citrate-containing wastewater is used as electron donor for sulfate reduction in a biological treatment plant for the removal of sulfate. The pathway of citrate conversion coupled to sulfate reduction and the microorganisms involved were investigated. Citrate was not a direct electron donor for the sulfate-reducing bacteria. Instead, citrate was fermented to mainly acetate and formate. These fermentation products served as electron donors for the sulfate-reducing bacteria. Sulfate reduction activities of the reactor biomass with acetate and formate were sufficiently high to explain the sulfate reduction rates that are required for the process. Two citrate-fermenting bacteria were isolated. Strain R210 was closest related to Trichococcus pasteurii (99.5% ribosomal RNA (rRNA) gene sequence similarity). The closest relative of strain S101 was Veillonella montepellierensis with an rRNA gene sequence similarity of 96.7%. Both strains had a complementary substrate range

Lack of an Antibacterial Response Defect in Drosophila Toll-9 Mutant

Toll and Toll-like receptors represent families of receptors involved in mediating innate immunity response in insects and mammals. Although Drosophila proteome contains multiple Toll paralogs, Toll-1 is, so far, the only receptor to which an immune role has been attributed. In contrast, every single mammalian TLR is a key membrane receptor upstream of the vertebrate immune signaling cascades. The prevailing view is that TLR-mediated immunity is ancient. Structural analysis reveals that Drosophila Toll-9 is the most closely related to vertebrate TLRs and utilizes similar signaling components as Toll-1. This suggests that Toll-9 could be an ancestor of TLR-like receptors and could have immune function. Consistently, it has been reported that over-expression of Toll-9 in immune tissues is sufficient to induce the expression of some antimicrobial peptides in flies. These results have led to the idea that Toll-9 could be a constitutively active receptor that maintain significant levels of antimicrobial molecules and therefore provide constant basal protection against micro-organisms. To test theses hypotheses, we generated and analyzed phenotypes associated with a complete loss-of-function allele of Toll-9. Our results suggest that Toll-9 is neither required to maintain a basal anti-microbial response nor to mount an efficient immune response to bacterial infection

Pigment epithelium-derived factor protects retinal ganglion cells

BACKGROUND: Retinal ganglion cells (RGCs) are responsible for the transmission of visual signals to the brain. Progressive death of RGCs occurs in glaucoma and several other retinal diseases, which can lead to visual impairment and blindness. Pigment epithelium-derived factor (PEDF) is a potent antiangiogenic, neurotrophic and neuroprotective protein that can protect neurons from a variety of pathologic insults. We tested the effects of PEDF on the survival of cultured adult rat RGCs in the presence of glaucoma-like insults, including cytotoxicity induced by glutamate or withdrawal of trophic factors. RESULTS: Cultured adult rat RGCs exposed to glutamate for 3 days showed signs of cytotoxicity and death. The toxic effect of glutamate was concentration-dependent (EC(50 )= 31 μM). In the presence of 100 μM glutamate, RGC number decreased to 55 ± 4% of control (mean ± SEM, n = 76; P < 0.001). The glutamate effect was completely eliminated by MK801, an NMDA receptor antagonist. Trophic factor withdrawal also caused a similar loss of RGCs (54 ± 4%, n = 60, P < 0.001). PEDF protected against both insults with EC(50 )values of 13.6 ng/mL (glutamate) and 3.4 ng/mL (trophic factor withdrawal), respectively. At 100 ng/mL, PEDF completely protected the cells from both insults. Inhibitors of the nuclear factor κB (NFκB) and extracellular signal-regulated kinases 1/2 (ERK1/2) significantly reduced the protective effects of PEDF. CONCLUSION: We demonstrated that PEDF potently and efficaciously protected adult rat RGCs from glutamate- and trophic factor withdrawal-mediated cytotoxicity, via the activation of the NFκB and ERK1/2 pathways. The neuroprotective effect of PEDF represents a novel approach for potential treatment of retinopathies, such as glaucoma

Chromosomal Rearrangements Formed by rrn Recombination Do Not Improve Replichore Balance in Host-Specific Salmonella enterica Serovars

operons. One hypothesis explaining these rearrangements suggests that replichore imbalance introduced from horizontal transfer of pathogenicity islands and prophages drives chromosomal rearrangements in an attempt to improve balance.This hypothesis was directly tested by comparing the naturally-occurring chromosomal arrangement types to the theoretically possible arrangement types, and estimating their replichore balance using a calculator. In addition to previously characterized strains belonging to host-specific serovars, the arrangement types of 22 serovar Gallinarum strains was also determined. Only 48 out of 1,440 possible arrangement types were identified in 212 host-specific strains. While the replichores of most naturally-occurring arrangement types were well-balanced, most theoretical arrangement types had imbalanced replichores. Furthermore, the most common types of rearrangements did not change replichore balance.The results did not support the hypothesis that replichore imbalance causes these rearrangements, and suggest that the rearrangements could be explained by aspects of a host-specific lifestyle

Codon Preference Optimization Increases Heterologous PEDF Expression

Pigment epithelium-derived factor (PEDF) is widely known for its neurotrophic and antiangiogenic functions. Efficacy studies of PEDF in animal models are limited because of poor heterologous protein yields. Here, we redesigned the human PEDF gene to preferentially match codon frequencies of E coli without altering the amino acid sequence. Following de novo synthesis, codon optimized PEDF (coPEDF) and the wtPEDF genes were cloned into pET32a containing a 5′ thioredoxin sequence (Trx) and the recombinant Trx-coPEDF or Trx-wtPEDF fusion constructs expressed in native and two tRNA augmented E coli hosts - BL21-CodonPlus(DE3)-RIL and BL21-CodonPlus(DE3)-RP, carrying extra copies of tRNAarg,ile,leu and tRNAarg,pro genes , respectively. Trx-PEDF fusion proteins were isolated using Ni-NTA metal affinity chromatography and PEDF purified after cleavage with factor Xα. Protein purity and identity were confirmed by western blot, MALDI-TOF, and UV/CD spectral analyses. Expression of the synthetic gene was ∼3.4 fold greater (212.7 mg/g; 62.1 mg/g wet cells) and purified yields ∼4 fold greater (41.1 mg/g; 11.3 mg/g wet cell) than wtPEDF in the native host. A small increase in expression of both genes was observed in hosts supplemented with rare tRNA genes compared to the native host but expression of coPEDF was ∼3 fold greater than wtPEDF in both native and codon-bias-adjusted E coli strains. ΔGs at −3 to +50 of the Trx site of both fusion genes were −3.9 kcal/mol. Functionally, coPEDF was equally as effective as wtPEDF in reducing oxidative stress, promoting neurite outgrowth, and blocking endothelial tube formation. These findings suggest that while rare tRNA augmentation and mRNA folding energies can significantly contribute to increased protein expression, preferred codon usage, in this case, is advantageous to translational efficiency of biologically active PEDF in E coli. This strategy will undoubtedly fast forward studies to validate therapeutic utility of PEDF in vivo