ZEBRAFISH: AN EMERGING MODEL SYSTEM FOR DRUG DISCOVERY

ABSTRACTThe zebrafish is no doubt a powerful model organism with a combination of forward and reverse genetics, low cost, amenable high throughput, andrapid in vivo analysis. With these unique features, it can be expected that the zebrafish will become more frequently used for drug discovery. Thisreview outlines the potential of zebrafish to contribute to drug discovery through the identification of novel drug targets, validation of those targetsand screening for new therapeutic compounds and assay development.Keywords: Zebrafish, Drug screening, Drug target, Development

Finite element analysis of cracking and delamination of concrete beam due to steel corrosion

This paper presents the analytical results to investigate cracking and delamination of concrete beam due to steel corrosion. A series of concrete beams were idealised as two dimensional models via their cross section and analysed using the finite element software – LUSAS. The corrosion of steel bars was simulated using a radial expansion. The FE results show that cracking of beam section due to steel corrosion can be clarified into four types, i.e., Internal Cracking, Internal Penetration, External Cracking (HS) and External Cracking (VB). The amount of corrosion in term of radial expansion required to causes Internal Cracking, Internal Penetration, External Cracking (HS) and External Cracking (VB) varies almost linearly with bar diameter d, bar clear distance s and concrete cover c, respectively. If the ratio s/c was less than the critical value of about 2.2, the delamination of concrete cover could occur before the cracks can be visualised on the concrete surface, which does concern engineers

EXPERIMENTAL AND THEORETICAL VALIDATION OF DOUBLE COLUMN INTERNAL FIXATION THEORY FOR DISTAL FEMORAL FRACTURES

Purpose of the study – to experimentally compare efficiency of lateral and bilateral (lateral and medial) plate fixation of distal femoral fractures and to validate the appropriateness of double column theory of distal femur anatomy for internal fixation for these fractures.Material and methods. The authors performed a biomechanical study in two series on polyurethane models of right femur corresponding in dimensions to natural femur. After simulating a 33 C2 fracture type it was fixed by one lateral plate or two (lateral and medial) plates. After fixation the models were tested in six load ranges with maximal load from 20 to 120 kgf in cyclic mode.Results. The authors obtained a significant difference in absolute values of fragments displacement amplitude depending on fixation method. In the first series of the experiment – one plate fixed on the lateral surface of the femur – under minimal load the displacement value was reported as0.3 mm and under maximal load —1.9 mm; in the second series of experiment – two plates fixed on the lateral and medial surfaces of the femur – displacement values were reported as0.35 mm and0.95 mm respectively. Conclusion. The presence or absence of medial support after internal fixation has a profound impact on ensuring stability in cases of comminuted fractures of distal femoral fractures. In this context the use of double column theory of distal femur anatomy for internal fixation can significantly improve the treatment outcomes for such patients. After trials of minimally invasive fixation method on anatomical specimen the described theory can be implemented into the clinical practice