Responsive glyco-poly(2-oxazoline)s: synthesis, cloud point tuning, and lectin binding

A new sugar-substituted 2-oxazoline monomer was prepared using the copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction. Its copolymerization with 2-ethyl-2-oxazoline as well as 2-(dec-9-enyl)-2-oxazoline, yielding well-defined copolymers with the possibility to tune the properties by thiol-ene "click" reactions, is described. Extensive solubility studies on the corresponding glycocopolymers demonstrated that the lower critical solution temperature behavior and pH-responsiveness of these copolymers can be adjusted in water and phosphate-buffered saline (PBS) depending on the choice of the thiol. By conjugation of 2,3,4,6-tetra-O-acetyl-1-thio-beta-D-glucopyranose and subsequent deprotection of the sugar moieties, the hydrophilicity of the copolymer could be increased significantly, allowing a cloud-point tuning in the physiological range. Furthermore, the binding capability of the glycosylated copoly(2-oxazoline) to concanavalin A was investigated

Coulomb and nuclear breakup of a halo nucleus 11Be

Breakup reactions of the one-neutron halo nucleus 11Be on Pb and C targets at about 70 MeV/u have been investigated by measuring the momentum vectors of the incident 11Be, outgoing 10Be, and neutron in coincidence. The relative energy spectra as well as the angular distributions of the 10Be+n center of mass have been extracted for both targets. For the breakup on Pb target, the selection of forward scattering angles is found to be effective to extract almost purely the first-order E1 Coulomb breakup component, and to exclude the nuclear contribution and higher-order Coulomb breakup components. This angle-selected energy spectrum is thus used to deduce the spectroscopic factor for the 10Be(0+) 2s_1/2 configuration in 11Be which is found to be 0.72+-0.04 with B(E1) up to Ex=4 MeV of 1.05+-0.06 e2fm2. The energy weighted E1 strength up to Ex=4 MeV explains 70+-10% of the cluster sum rule, consistent with the obtained spectroscopic factor. The non-energy weighted sum rule is used to extract the root mean square distance of the halo neutron to be 5.77(16) fm, consistent with previously known values. In the breakup with C target, we have observed the excitations to the known unbound states in 11Be at Ex=1.78 MeV and 3.41 MeV. Angular distributions for these states show the diffraction pattern characteristic of L=2 transitions, resulting in J^pi =(3/2,5/2)+ assignment for these states. We finally find that even for the C target the E1 Coulomb direct breakup mechanism becomes dominant at very forward angles.Comment: 14 pages, 7 figures, accepted for publication on Physical Review

Impaired Cerebral Autoregulation Is Associated with Brain Atrophy and Worse Functional Status in Chronic Ischemic Stroke

Dynamic cerebral autoregulation (dCA) is impaired following stroke. However, the relationship between dCA, brain atrophy, and functional outcomes following stroke remains unclear. In this study, we aimed to determine whether impairment of dCA is associated with atrophy in specific regions or globally, thereby affecting daily functions in stroke patients. We performed a retrospective analysis of 33 subjects with chronic infarctions in the middle cerebral artery territory, and 109 age-matched non-stroke subjects. dCA was assessed via the phase relationship between arterial blood pressure and cerebral blood flow velocity. Brain tissue volumes were quantified from MRI. Functional status was assessed by gait speed, instrumental activities of daily living (IADL), modified Rankin Scale, and NIH Stroke Score. Compared to the non-stroke group, stroke subjects showed degraded dCA bilaterally, and showed gray matter atrophy in the frontal, parietal and temporal lobes ipsilateral to infarct. In stroke subjects, better dCA was associated with less temporal lobe gray matter atrophy on the infracted side ( = 0.029), faster gait speed ( = 0.018) and lower IADL score (0.002). Our results indicate that better dynamic cerebral perfusion regulation is associated with less atrophy and better long-term functional status in older adults with chronic ischemic infarctions