Implicit theories of emotion shape regulation of negative affect

Mental contrasting of a desired future with the present reality strengthens the link between expectations and goal pursuit: The higher expectations of success, the more people engage in goal pursuit; the lower expectations of success, the more people let go or disengage from goal pursuit. In three studies, we tested if mental contrasting increases the link between expectations and goal pursuit by affecting the strength of mental associations between future and reality. We used lexical decision tasks to measure the strength of associations between future and reality for different domains of goal pursuit (i.e., interpersonal relations, achievement), and compared results in the mental contrasting condition to relevant control conditions (i.e., reverse contrasting and content control). In the mental contrasting condition but not in the control conditions emerged a strong link between expectations of success and the strength of associations between future and reality (Study 1, 2). The strength of associations between future and reality in turn mediated the link between expectations and self-reported as well as other-rated goal pursuit in the mental contrasting condition (Study 1, 2). Finally, the link between expectations and the strength of associations between future and reality in the mental contrasting condition vanished when the goal was attained (Study 3). Taken together, these results suggest that strength of future–reality associations are a mechanism specific to mental contrasting effects on goal pursuit

Computational discovery of cis-regulatory modules in Drosophila without prior knowledge of motifs

Prediction of cis-regulatory modules ab initio, without any input of relevant motifs, is achieved with two novel methods

Social Networking and the Perception of Privacy within the Millennial Generation

Has technology caused a generational divide between current college age users (Millennial Generation) who have no problems posting intimate details of their personal life on the Web and more traditional older users who seem to value privacy? This paper presents the results of a survey of 251 university students and follow-up focus groups on the topic of the perceptions of social networking and privacy. We will use Facebook as an example of social networking, and review attitudes about privacy and control over personal information among traditional and non-traditional college age users and light and heavy users of social networking sites

Cleaved Caspase-3 Sebagai Uji Apoptosis Pada Kanker Serviks IIB Tipe Sel Skuamosa Yang Mendapat Kemoterapi Neoadjuvan Cisplatin

Tujuan: mencari peningkatan ekspresi cleaved caspase-3 pada pasien kanker serviks IIB tipe sel skuamosa sesudah pemberian kemoterapi neoadjuvan cisplatin dan mencari kapan waktu yang tepat untuk mendeteksi apoptosis menggunakan cleaved caspase-3. Bahan dan Metode: Jenis penelitian analitik observasional berpasangan. Dilakukan pada penderita kanker serviks IIB tipe sel skuamosa berdasarkan kriteria FIGO yang berobat di POSA RSUD Dr. Soetomo Surabaya. Penelitian dilakukan mulai bulan Mei 2014. Sebelum dilakukan terapi semua penderita diambil biopsi serviks. Kemudian penderita diberikan cisplatin 50mg/m2/minggu Diambil biopsi serviks setelah diberikan kemoterapi ke 1 dan ke 4 (maksimal 8 jam setelah kemoterapi, sampel kedua). Sampel diperiksakan imunohistokimia cleaved caspase 3. Dilakukan penghitungan ekspresi cleaved caspase 3 di bawah mikroskop dengan perbesaran 400x pada 10 lapang pandang. Pengujian statistik dilakukan dengan nilai kemaknaan p<0,05.Hasil: Rata-rata ekspresi cleaved caspase 3 sebelum kemoterapi adalah 1,46± 1,854. Setelah kemoterapi ke 1 didapatkan rata- rata 10,77± 3,655.Setelah kemoterapi ke 4 didapatkan rata- rata 12,77± 5,703. Pada pemeriksaan didapatkan ekspresi cleaved caspase 3 setelah pemberian kemoterapi lebih meningkat dibandingkan sebelum pemberian kemoterapi (p<0,01), sedangkan ekspresi cleaved caspase 3 setelah kemoterapi ke 1 dan ke 4 tidak didapat-kan perbedaan bermakna (p=0,882).Simpulan: Pemberian kemoterapi terbukti memberikan efek peningkatan ekspresi cleaved caspase-3. Sehingga cleaved caaspase-3 dapat dijadikan sebagai salah satu uji apoptosis pada efek kemoterapi terhadap sel kanker

Developing Strategic Partnerships to Impact Population Health: A Professional Development Module

Massive organizational changes occurring in our health care delivery system have provided opportunities for nurse leaders who think in terms of population health, to develop new skills needed to impact the populations they serve. Aspects of these changes include a focus on the delivery of primary and community-based care and the development of partnerships, alliances, and other linkages across settings to build integrated systems (Williams, 2020, p. 17). Turner (2020) asserts that population-focused nurse leaders are charged with creating a new future for healthier communities which often involves strategic partnership development. However, strategic partnership development is a complex task that requires much time, energy, and thoughtful attention (p. 881). The purpose of this Doctoral of Nursing (DNP) project was to develop, implement, and evaluate an online module for nurse leaders to increase knowledge and self-efficacy related to developing, growing, and sustaining strategic partnerships to impact population health. An online module was designed by this DNP student that includes five major content areas; 1) Understanding the importance of strategic partnership development to impact population health in the current health care environment, 2) The foundations of successful partnerships that can be used across multiple disciplines, 3) How to examine potential partnerships for fit, relevancy, and achieving established goals, 4) How to grow and sustain partnerships that have been successfully implemented, and 5) The importance of continuous evaluation of strategic partnerships that have been implemented. The module was delivered in an online format that offered 1.5 hours of continuing education for busy nurse leaders who work in various healthcare settings

Low pre-stimulus EEG alpha power amplifies visual awareness but not visual sensitivity

Pre‐stimulus oscillatory neural activity has been linked to the level of awareness of sensory stimuli. More specifically, the power of low frequency oscillations (primarily in the alpha‐band, i.e. 8‐14 Hz) prior to stimulus‐onset is inversely related to measures of subjective performance in visual tasks, such as confidence and visual awareness. Intriguingly, the same EEG‐signature does not seem to influence objective measures of task performance (i.e. accuracy). We here examined whether this dissociation holds when stringent accuracy measures are used. Previous EEG‐studies have employed 2‐alternative forced‐choice (2‐AFC) discrimination tasks to link pre‐stimulus oscillatory activity to correct/incorrect responses as an index of accuracy/objective performance at the single‐trial level. However, 2‐AFC tasks do not provide a good estimate of single‐trial accuracy, as many of the responses classified as correct will be contaminated by guesses (with the chance correct response rate being 50%). Here instead, we employed a 19‐AFC letter identification task to measure accuracy and the subjectively reported level of perceptual awareness on each trial. As the correct guess rate is negligible (~5%), this task provides a purer measure of accuracy. Our results replicate the inverse relationship between pre‐stimulus alpha/beta‐band power and perceptual awareness ratings in the absence of a link to discrimination accuracy. Pre‐stimulus oscillatory phase did not predict either subjective awareness or accuracy. Our results hence confirm a dissociation of the pre‐stimulus EEG power ‐ task performance link for subjective versus objective measures of performance, and further substantiate pre‐stimulus alpha power as a neural predictor of visual awareness

Deconvolution of complex G protein-coupled receptor signaling in live cells using dynamic mass redistribution measurements

Label-free biosensor technology based on dynamic mass redistribution (DMR) of cellular constituents promises to translate GPCR signaling into complex optical 'fingerprints' in real time in living cells. Here we present a strategy to map cellular mechanisms that define label-free responses, and we compare DMR technology with traditional second-messenger assays that are currently the state of the art in GPCR drug discovery. The holistic nature of DMR measurements enabled us to (i) probe GPCR functionality along all four G-protein signaling pathways, something presently beyond reach of most other assay platforms; (ii) dissect complex GPCR signaling patterns even in primary human cells with unprecedented accuracy; (iii) define heterotrimeric G proteins as triggers for the complex optical fingerprints; and (iv) disclose previously undetected features of GPCR behavior. Our results suggest that DMR technology will have a substantial impact on systems biology and systems pharmacology as well as for the discovery of drugs with novel mechanisms